Differences In Adiponectin Levels Research Articles

Preliminary Report on the Influence of Acute Inflammation on Adiponectin Levels in Older Inpatients with Different Nutritional Status.

Inflammation can be triggered by a variety of factors, including pathogens, damaged cells, and toxic compounds. It is a biological response of the immune system, which can be successfully assessed in clinical practice using some molecular substances. Because adiponectin, a hormone released by adipose tissue, influences the development of inflammation, its evaluation as a potential measure of inflammation in clinical practice is justified. In the present contribution, statistical comparison of adiponectin concentration and selected molecular substances recognized in clinical practice as measures of inflammation were utilized to demonstrate whether adipose tissue hormones, as exemplified by adiponectin, have the potential to act as a measure of rapidly changing inflammation when monitoring older hospitalized patients in the course of bacterial infection. The study showed no statistically significant differences in adiponectin levels depending on the rapidly changing inflammatory response in its early stage. Interestingly, the concentration of adiponectin is statistically significantly higher in malnourished patients than in people with normal nutritional levels, assessed based on the MNA. According to the results obtained, adiponectin is not an effective measure of acute inflammation in clinical practice. However, it may serve as a biomarker of malnutrition in senile individuals.

Exploring the Relationship between Plasma Adiponectin, Gender, and Underlying Diseases in Severe Illness.

Adiponectin is low in obesity, plays a crucial role in metabolic health, and, moreover, possesses immunoregulatory properties. However, studies examining its levels in patients with systemic inflammatory response syndrome (SIRS) or sepsis have yielded conflicting results. While females typically have higher systemic adiponectin levels than males, research on sex-specific associations in this context is limited. In this study of 156 SIRS/sepsis patients, including those with liver cirrhosis, we aimed to explore the relationship between plasma adiponectin, body mass index (BMI), gender, disease severity, and underlying etiological conditions. Our findings revealed that patients with liver cirrhosis, who are susceptible to infections, exhibited elevated circulating adiponectin levels, irrespective of sex. When excluding cirrhosis patients, plasma adiponectin levels were similar between male SIRS/sepsis patients and controls but lower in female patients compared to female controls. Plasma adiponectin was inversely related to BMI in female but not male patients. Further analysis within the non-cirrhosis subgroup demonstrated no significant differences in adiponectin levels between sexes among SIRS, sepsis, and septic shock patients. Ventilation, dialysis, and vasopressor therapy had no discernible impact on adiponectin levels in either sex. A negative correlation between adiponectin and C-reactive protein (CRP) existed in males only. Notably, patients with pancreatitis showed the lowest plasma adiponectin concentrations, although sex-specific differences were not significant. Infection with Gram-negative or Gram-positive bacteria had minimal effects on plasma adiponectin levels in both sexes. However, infection with the severe acute respiratory syndrome coronavirus type 2 led to decreased adiponectin levels in females exclusively. Multivariate analysis considering all factors affecting plasma adiponectin levels in males or females identified BMI in females and CRP levels in males to predict plasma adiponectin levels in SIRS/sepsis patients. Additionally, our study observed a trend where the 25 patients who did not survive had higher plasma adiponectin levels, particularly among males. In summary, our investigation highlights the influence of underlying diseases and sex on plasma adiponectin levels in SIRS/sepsis patients, shedding light on potential implications for disease management and prognosis.

Comparison of Adiponectin Levels in Anorexia Nervosa, Bulimia Nervosa, Binge-Eating Disorder, Obesity, Constitutional Thinness, and Healthy Controls: A Network Meta-Analysis.

Adiponectin is a protein hormone that is produced and secreted primarily by adipose tissue. The levels of adiponectin in those with eating disorders, obesity, and healthy controls have been extensively studied. However, the general picture of the differences in adiponectin levels across the mentioned conditions is still unclear and fragmented. In this study, we pooled previous studies and performed a network meta-analysis to gain a global picture of comparisons of adiponectin levels across eating disorders, obesity, constitutional thinness, and healthy controls. Electronic databases were searched for anorexia nervosa, avoidant restrictive food intake disorder, binge-eating disorder, bulimia nervosa, healthy controls, night eating syndrome, obesity, and constitutional thinness in studies where adiponectin levels were measured. A total of 4262 participants from 50 published studies were included in the network meta-analysis. Adiponectin levels were significantly higher in participants with anorexia nervosa than in healthy controls (Hedges' g = 0.701, p < 0.001). However, adiponectin levels in constitutionally thin participants were not significantly different from those of healthy controls (Hedges' g = 0.470, p = 0.187). Obesity and binge-eating disorder were associated with significantly lower adiponectin levels compared to those of healthy controls (Hedges' g = -0.852, p < 0.001 and Hedges' g = -0.756, p = 0.024, respectively). The disorders characterized by excessive increases or decreases in BMI were associated with significant changes in adiponectin levels. These results suggest that adiponectin may be an important marker of severely disequilibrated homeostasis, especially in fat, glucose, and bone metabolisms. Nonetheless, an increase in adiponectin may not simply be associated with a decrease in BMI, as constitutional thinness is not associated with a significant increase in adiponectin.

Elevated baseline adiponectin level predicting an increased risk of disease activity in idiopathic nephrotic syndrome and chronic kidney disease in children

Adiponectin, an adipocyte-derived protein, is present abundantly in circulation and has anti-atherogenic and anti-inflammatory effects, so we aimed to assess the role of adiponectin in idiopathic nephrotic syndrome and chronic kidney disease in children. Overall of 290 children, 95 patients with the steroid-responsive idiopathic nephrotic syndrome (50 in remission and 45 in relapse). Another 95 patients with chronic kidney disease (50 on hemodialysis and 45 on conservative therapies) and 100 apparently healthy matched children were enrolled into the study. Serum adiponectin levels, blood chemistry (protein/albumin, serum creatinine, blood urea and electrolytes levels) and 24-hour proteinuria w studied as well. There was highly significant increasing differences in adiponectin level between the relapsed idiopathic nephrotic syndrome (26.93±6.79 µg/mL) compared to remission (10.96±3.33 µg/mL) and control group (4.89±1.01 µg/mL) and between remission in comparing to control group (P<0.001). Also there was significant negative correlation between adiponectin level and serum albumin level in the relapse group (r=-0.60, P≤0.05), and highly positive correlation between adiponectin level and 24-hour proteinuria in relapse (r=0.98, P≤0.001) and remission group (r=0.92, P≤0.001). Regarding children with chronic kidney disease, There were high significant increasing differences in adiponectin levels in conservative (13.88±4.27 µg/mL) and dialysis (15.09±6.14 µg/mL) groups compared to controls (6.05±5.83 µg/mL) with P<0.001. Furthermore, there was a significant positive correlation between adiponectin and serum creatinine and blood urea levels in both conservative and dialysis groups with P<0.05. Adiponectin can be considered as an important useful biomarker for chronic kidney diseases in children.

ADIPONECTIN LEVELS IN GRAVES' DISEASE - SYSTEMATIC REVIEW AND META-ANALYSIS.

Graves' disease is the most prevalent cause of hyperthyroidism worldwide. Adiponectin, the most abundant adipokine, plays a significant role in a cluster of prevalent diseases connected to metabolic disorders. Although the association between adiponectin and Graves' disease has been studied, the existing data is inconsistent. Therefore, we conducted this systematic review and meta-analysis to evaluate the relationship between adiponectin levels and Graves' disease. We performed a systematic electronic search on PubMed, EMBASE, Scopus and Cochrane Library using predefined keywords. We used the NHLBI quality assessment tools to assess the included studies. There were 11 studies involving 781 subjects included in our qualitative synthesis, while 6 studies were included in our quantitative synthesis. We observed significantly increased adiponectin levels in Graves' disease patients compared to controls (MD 2.983 [95% CI 0.138-5.828]) and hypothyroidism patients (MD 3.389 [95% CI 1.332-5.446]). Nevertheless, no significant MD was observed when comparing Graves' disease patients with and without Graves' ophthalmopathy (MD -27.124 [95% CI -88.893 - 34.645]). Adiponectin levels were significantly higher in patients with Graves' disease compared to controls and hypothyroidism patients. However, patients with and without Graves' ophthalmopathy did not present a significant mean difference in adiponectin levels.

Decreased Adiponectin Levels in Early Pregnancy Are Associated with High Risk of Prematurity for African American Women.

The relationship of low maternal serum adiponectin levels with preterm delivery among a multi-ethnic group has not been extensively investigated. We examined ethnic differences in cytokine/adipokine profiles and whether they contribute to several adverse pregnancy outcomes, particularly preterm delivery. Data and samples were from a large prospective observational cohort (n = 1776) of young, generally healthy pregnant women (African American 36.4%, Hispanic 48.0%, Caucasian 15.6%). Serum cytokine/adipokine concentrations were measured at entry (mean gestational age of 16.83 weeks) using the Liminex xMap Technology. Multivariable analyses were performed. A significant difference in adiponectin level was observed among ethnic groups. African Americans had a decreased adiponectin and increased resistin levels compared to Hispanics and Caucasians (p < 0.05 to p < 0.0001 for each). Decreased adiponectin (lowest quartile) was positively associated with preterm delivery independent of usual risk factors (adjusted odds ratio (AOR) 1.46, 95% confidence interval (CI) 1.05, 2.04 for all preterm and AOR 1.84, 95% CI 1.07, 3.17 for early preterm births). The results were unchanged when women with preeclampsia were excluded. Similar results were observed in African Americans. Decreased adiponectin levels were not related to preterm birth in either Hispanics or Caucasians. Lower adiponectin levels were also significantly associated with an increased risk of developing gestational diabetes (AOR 1.72, 95% CI 1.05, 2.84) and preeclampsia (AOR 1.45, 95% CI 1.00, 2.14) in the whole cohort and in Caucasians. We did not find any consistent relationships between the other markers with outcome variables. Dysregulation in maternal adiponectin at early gestation is associated with an increased risk of preterm delivery. An ethnic difference in adiponectin levels may contribute to a higher preterm delivery rate in African American women.

DDAH1/ADMA Regulates Adiponectin Resistance in Cerebral Ischemia via the ROS/FOXO1/APR1 Pathway.

Dimethylarginine dimethylaminohydrolase 1 (DDAH1) protects against cerebral ischemia injury via regulating the level of asymmetric dimethylarginine (ADMA). This study is aimed at exploring the effect of adiponectin resistance on ADMA-induced neuronal loss in ischemic stroke (IS) and the underlying mechanism. DDAH1 knockout (DDAH1−/−) and wild-type (DDAH1+/+) rats underwent middle cerebral artery occlusion/reperfusion (MCAO/R). Plasma and brain adiponectin levels and the expressions of adiponectin receptor 1 (APR1), adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1 (APPL1), adenosine monophosphate-activated protein kinase (AMPK), and phosphorylated AMPK were determined after 24 h, 3 days, and 7 days. Neurological behavior, infarct volume, and adiponectin signaling were evaluated using adiponectin peptide or AdipoRon. The levels of reactive oxygen species (ROS) and Forkhead box O1 (FOXO1) (a transcription factor for APR1) were also assessed. An oxygen-glucose deprivation/reoxygenation (OGD/R) model was established in primary neurons. DDAH1 was overexpressed in neurons, after which FOXO1 expression, ROS production, adiponectin resistance, and cell viability were detected. DDAH1−/− rats showed no significant difference in adiponectin level in either plasma or brain after MCAO/R in DDAH1+/+ rats, but downregulated APR1 expression and suppressed adiponectin signaling were observed. AdipoRon, but not adiponectin peptide, attenuated the neurological deficits and adiponectin resistance in DDAH1−/− rats. ROS accumulation and phosphorylated FOXO1 expression also increased with DDAH1 depletion. Following DDAH1 overexpression, decreased cell viability and inhibited adiponectin signaling induced by OGD/R were alleviated in primary neurons, accompanied by reduced ROS production and phosphorylated FOXO1 expression. Our study elucidated that in IS, DDAH1 protected against adiponectin resistance in IS via the ROS/FOXO1/APR1 pathway.

Puberty Status Modifies the Effects of Genetic Variants, Lifestyle Factors and Their Interactions on Adiponectin: The BCAMS Study.

BackgroundHypoadiponectinemia has been associated with various cardiometabolic disease states. Previous studies in adults have shown that adiponectin levels were regulated by specific genetic and behavioral or lifestyle factors. However, little is known about the influence of these factors on adiponectin levels in children, particularly as mitigated by pubertal development.MethodsWe performed a cross-sectional analysis of data from 3,402 children aged 6-18 years from the Beijing Child and Adolescent Metabolic Syndrome (BCAMS) study. Pubertal progress was classified as prepubertal, midpuberty, and postpuberty. Six relevant single nucleotide polymorphisms (SNPs) were selected from previous genome-wide association studies of adiponectin in East Asians. Individual SNPs and two weighted genetic predisposition scores, as well as their interactions with 14 lifestyle factors, were analyzed to investigate their influence on adiponectin levels across puberty. The effect of these factors on adiponectin was analyzed using general linear models adjusted for age, sex, and BMI.ResultsAfter adjustment for age, sex, and BMI, the associations between adiponectin levels and diet items, and diet score were significant at prepuberty or postpuberty, while the effect of exercise on adiponectin levels was more prominent at mid- and postpuberty. Walking to school was found to be associated with increased adiponectin levels throughout puberty. Meanwhile, the effect of WDR11-FGFR2-rs3943077 was stronger at midpuberty (P = 0.002), and ADIPOQ-rs6773957 was more effective at postpuberty (P = 0.005), while CDH13-rs4783244 showed the strongest association with adiponectin levels at all pubertal stages (all P < 3.24 × 10-15). We further found that effects of diet score (P interaction = 0.022) and exercise (P interaction = 0.049) were stronger in children with higher genetic risk of hypoadiponectinemia, while higher diet score and exercise frequency attenuated the differences in adiponectin levels among children with different genetic risks.ConclusionsOur study confirmed puberty modulates the associations between adiponectin, and genetic variants, lifestyle factors, and gene-by-lifestyle interactions. These findings provide new insight into puberty-specific lifestyle suggestions, especially in genetically susceptible individuals.

Plasma Lipocalin-2 and Adiponectin are Affected by Obesity Rather Than Type 2 Diabetes Mellitus per se.

PurposeChanges in plasma adipocytokines and inflammatory markers in type 2 DM remain controversial as to whether they are due to obesity or directly associated with the diabetic state. Our objective was to study the effect of obesity and diabetes on plasma lipocalin-2 (LCN2), adiponectin, and interleukin-1β (IL-1β) by comparing their levels in non-diabetic obese subjects and non-obese type 2 DM patients, as well as determining the association of these adipocytokines with metabolic syndrome factors and diabetic parameters.Patients and MethodsIn this study, 85 Yemeni male volunteers aged 30–60 years old were enrolled, 25 of whom were healthy subjects with BMI < 25 kg/m2 served as control; 30 non-diabetic obese subjects (BMI ≥ 30 kg/m2 and FBG < 6.1 mmol/l); and 30 non-obese type 2 DM patients (BMI < 25 kg/m2 and FBG > 7 mmol/l).ResultsLipocalin-2 and adiponectin were significantly (p = 0.043 and p = 0.034) lower in non-diabetic obese subjects by 16.2% and 29.7% with respect to control group, with no effect in the non-obese type 2 DM patients. Moreover, LCN2 was significantly (p = 0.04) lower in the non-diabetic obese subjects by 15.8% as compared with the non-obese type 2 DM patients, with no significant difference in adiponectin levels. In contrast, serum IL-1β was significantly (p = 0.001 and p = 0.003) higher in both non-diabetic obese subjects and the non-obese type 2 DM patients by 76.5% and 67.7% as compared to control group. The significant decrease in both LCN2 and adiponectin and the significant increase in IL-1β in the non-diabetic obese subjects disappeared upon adjustment for waist circumference (WC). In contrast, the significant increase in IL-1β in the non-obese Type 2 DM patients was not affected upon adjustment for WC.ConclusionPlasma LCN2 and adiponectin were not affected by diabetes per se, suggesting that the observed changes in LCN2 and adiponectin in type 2 DM may be due to obesity rather than the diabetic state, whereas IL-1β levels were affected by both obesity and diabetes.

Dietary Omega-6 to Omega-3 Fatty Acids Ratio is Correlated with High Molecular Weight Adiponectin Level in Indonesian Office Workers

Context: Adiponectin is an adipokine that is secreted by adipocytes and acts to prevent obesity and obesity-related disorders. The high ratio of omega-6 to omega-3 fatty acids in modern dietary habits in working-age populations, urban areas in particular, is known to play a role in adiponectin secretion. However, previous studies showed inconclusive results. Aim: The study aims to determine the association of the dietary omega-6 to omega-3 fatty acids ratio with adiponectin levels in office workers in Jakarta, Indonesia. Settings and Design: This cross-sectional study included 55 office workers in East Jakarta, Indonesia. Methods: Data were collected via questionnaire, 3-day food record, anthropometric measurement, and serum collection. Statistical analysis used: Independent t test was applied to assess the relationship between subjects characteristics and dietary intake with high molecular weight adiponectin levels. Association of dietary omega-6/omega-3 fatty acids ratio and adiponectin were evaluated using Pearson correlation test (P < 0.05). Results: There is no statistically significant difference in adiponectin levels based on waist circumference category, as well as categories of physical activity, household income levels, total energy, and total fat intake. Conversely, in female subjects (P = 0.000), subjects with normal body mass index (P = 0.000), higher education levels (P = 0.031), and nonsmoking subjects (P = 0.019), significantly higher adiponectin levels are obtained. The ratio of dietary omega-6/omega-3 fatty acids is negatively correlated with adiponectin (P = 0.004, r = –0.383). Conclusions: The decrease in the ratio of omega-6/omega-3 fatty acids is inversely related to higher level of adiponectin, indicating that dietary changes may potentially play a role in prevention strategies for obesity and obesity-related diseases.

Does adiponectin play a role in the pathogenesis of chronic spontaneous urticaria?

IntroductionChronic spontaneous urticaria constitutes an interdisciplinary problem and its pathogenesis is still a subject of debate. Overweight and hyperlipidemia are supposed to be related to chronic spontaneous urticaria. Fatty tissue can be the source of adipokines.Aim of the studyTo assess the potential role of adiponectin in chronic spontaneous urticaria pathogenesis.Material and methodsThe study included 52 chronic spontaneous urticaria patients and 43 healthy controls. The patients were divided into two subgroups: patients with wheals only, and patients with urticaria and an accompanying angioedema. The adiponectin concentration was measured in all studied subjects.ResultsNo statistically significant difference in adiponectin level was determined between the studied groups and subgroups.ConclusionsWe are among the first to present the results of study to determine a possible role of adipokines in chronic spontaneous urticaria pathogenesis. We did not observe any difference in adiponectin level. In our opinion, it is necessary to conduct further analyses in this field.

Adipocytokines in early rheumatoid arthritis: relationship to pro- and anti-inflammatory markers

Objective:to clarify the relationship of adiponectin and leptin to the signs of disease activity and the levels of pro- and anti-inflammatory cytokines in patients with early rheumatoid arthritis (RA).Subjects and methods.The investigation enrolled 27 RA patients who met the 2010 ACR/EULAR classification criteria and had never received glucocorticoids (GCs) or disease-modifying antirheumatic drugs. The median age of the patients was 56 [46; 64] years; the duration of the disease was 8 [6; 15] months. All the patients had moderate or high RA activity according to DAS28. The majority of the patients were seropositive for rheumatoid factor (88.9%) or anticyclic citrullinated peptide antibodies (96.3%). A control group included 30 gender-, age-, and body mass index (BMI)-matched people without inflammatory arthritis. Enzyme immunoassay was used to estimate the concentrations of adiponectin and leptin; XMAP multiplex assay was applied to measure the levels of interleukin-1β (IL-1β), IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17 and macrophage inflammation proteins (MIP), such as MIP-1α and MIP-1β.Results and discussion.In RA patients, adiponectin concentrations were higher (p&lt;0.001) and leptin levels and leptin/adiponectin (L/A) ratios were lower than those in the controls (p=0.04 and p&lt;0.001, respectively). In RA, there were direct correlations of leptin levels with concentration of IL-17 (r=0.4; p=0.03), IL-4 (r=0.39; p=0.04) and erythrocyte sedimentation rate (ESR) (r=0.3; p=0.05), as well as relationships of L/A ratios to ESR (r=0.38; p=0.05) and the levels of CRP (r=0.4; p=0.04) and MIP-1β (r=0.55; p=0.03). An increase in adiponectin concentrations was associated with a decrease in MIP-1β levels (r=-0.63; p&lt;0.01). In patients with BMI ≥25 kg/m2, leptin concentrations were comparable in RA patients and controls (p=0.1); the differences in adiponectin levels and L/A ratios remained in both cases (p&lt;0.001). This subgroup of patients with RA showed significant correlations between leptin and IL-17 levels (r=0.52; p=0.03), adiponectin and MIP-1β concentrations (r=-0.59; p=0.01), L/A ratios and MIP-1β levels (r=0.55; p=0.02).Conclusion.In early RA, there was a lower leptin synthesis and a higher adiponectin production. The correlations between the levels of adipocytokines, IL-17, and MIP1β, on the one hand, suggest that adipose tissue has an impact on systemic inflammation and, on the other, confirm that proinflammatory cytokines are involved in the development of insulin resistance and obesity.

Serum adiponectin levels in patients with rheumatoid arthritis

Background and Objective: Rheumatoid arthritis (RA) is a chronic inflammatory polyarthritis of common occurrence. Adiponectin is an adipocytokine shown to exert anti-inflammatory as well as pro-inflammatory effects. Our objective was to study the serum adiponectin levels in RA and explore its association with RA disease activity. Materials and Methods: Forty RA patients (newly diagnosed n = 20, RA in clinical remission n = 20) and thirty age-, gender-, and body mass index (BMI)-matched apparently healthy controls were included. Serum adiponectin levels were measured by enzyme-linked immunosorbent assay. RA clinical remission was defined by a Disease Activity Score 28 of Results: Serum adiponectin levels were significantly lower in RA patients compared to controls. Both groups of RA patients had lower adiponectin levels than controls. There was no difference in adiponectin levels between the two groups of RA patients. There was no association of adiponectin with disease activity or BMI. Conclusions: Serum adiponectin concentration is decreased in RA patients and was not associated with disease activity.

Effect of Aerobic and Anaerobic Exercises on Anthropometric Parameters, Chemerin and Adiponectin Levels in Non-Athletic Men

Background and Objectives: Several studies have been shown the beneficial effect of exercise on markers of health. This study was designed to evaluate the effect of two aerobic and anaerobic exercise protocols on triglyceride, cholesterol, anthropometric indices, chemerin, and adiponectin levels in non-athletic men. Methods: A total of 39 non-athletic men were recruited to the present study. According to protocol, participants in three categories including; aerobic, anaerobic, and control groups, which had endurance, speedy exercise, and without activity, respectively. The duration of interventional program was two months. Body weight, height, and waist circumference (WC) were determined. Body fat percent, waist-to-hip ratio (WHpR) and body mass index (BMI) were calculated. Dietary intake was recorded by 48-hours recall method. Briefly, Total cholesterol and triglyceride levels were determined by an automated analyzer. Serum levels chemerin and adiponectin were measured by enzyme-linked immune-sorbent assay (ELISA) by commercial kits. Data was analyzed using the SPSS statistical software version 16. P < 0.05 was considered as the level of significant. Results: No significant difference among groups was observed according to BMI, WC, and WHR. Significant difference was observed based on percent of body fat in the aerobic group. There was no significant difference in the cholesterol and triglyceride levels and daily calorie intake between groups. There was a significant difference in adiponectin level in three steps between two groups (P < 0.001). There was a significant difference on chemerin level between baseline training and detraining steps in aerobic groups as compared to control (P = 0.01). There was an increase in chemerin level between baseline, training, and detraining steps in anerobic groups (P = 0.01). A significant increase in adiponectin level was observed after training. Whilst, after detraining, it was decreased significantly (P < 0.001). Conclusions: Aerobic exercise caused chemerin levels to decrease significantly after training and detraining. Whereas it increased in anaerobic groups. Besides, adiponectin level significantly increased in aerobic and anaerobic groups. It seems that the difference in the type of activity between the two groups led to the changes in the above indicators that require more extensive studies.

Ethnic Variations in Adiponectin Levels and Its Association with Age, Gender, Body Composition and Diet: Differences Between Iranians, Indians and Europeans Living in Australia.

Adiponectin is an adipocyte-derived protein with anti-diabetic, anti-atherogenic and anti-inflammatory action, but there are few studies on its association with cardiovascular and metabolic risk factors in different ethnic groups in Australia. This cross-sectional study evaluated ethnic differences in adiponectin levels and its association with age, gender, body composition and diet in 89 adult Australians of European (n = 28), Indian (n = 28) and Iranian (n = 33) ancestries. Different measures of adiposity were assessed using the method of whole body dual energy X-ray absorptiometry(DEXA). Total adiponectin levels determined in Indians and Iranians were significantly lower than those in Europeans (p values < 0.001). There was no significant difference between the adiponectin levels in Indians and Iranians (p value > 0.05). There was no substantial change in the results after adjustment for potential confounders. Circulating levels of adiponectin was associated with age, truncal fat percentage, dietary glycemic index, glycemic load and carbohydrate intake, by correlation analysis (p values < 0.05). Using multiple linear regression analysis, a model including truncal fat percentage (p < 0.001), ethnicity (p = 0.001), age (p = 0.001) and dietary glycemic index (p = 0.04) could predict 50% of the variance in adiponectin levels (R2 = 0.504). Among different variables assessed, truncal fat percentage (in Indian and Iranian groups) and glycemic index (in European group) were the strongest predictors of serum adiponectin when data were analysed for three ethnic groups, separately. In conclusion, individuals with Iranian or Indian ancestries may have lower adiponectin levels compared to Europeans. Ethnicity was found as an independent factor affecting adiponectin levels. Our results also highlighted age, truncal adiposity and dietary glycemic index as other determinants of serum adiponectin, however the extent to which these factors influence adiponectin concentrations may vary across ethnicities.

ATHEROSCLEROSIS;

Objectives: To compare the adiponectin/leptin ratio and intima media thicknessbetween normal and atherosclerotic individuals. To find out the association of A/L ratio withIMT in atherosclerotic patients. Study Design: Comparative cross sectional study. Placeand Duration of Study: This study was conducted at department of Physiology, BMSI incollaboration with Radiology department, Jinnah Postgraduate Medical Centre Karachi, from16th December 2014 to 15th December 2015. Methodology: 160 subjects having Dopplerultrasound of neck were selected on the basis of pre-determined criteria. They were grouped incases i.e. atherosclerotic group (80 subjects) and control i.e. healthy group (80 subjects). Age&gt; 20 years, both genders, patients for ultrasound Doppler for neck region irrespective of theirprovisional diagnosis, patients suffering from co-morbid conditions like diabetes, hypertension,chronic kidney disease, chronic liver disease, asthma and COPD and Healthy individuals(Controls) were included in this study. Patients who fail to gave consent and patients whoseultrasound findings were not up to the mark required for diagnosis were excluded from thisstudy. Results: Out of 160 patients in this study in control Male: Female ratio was 1:1.1 whilein cases, Male: Female ratio = 1:1. Majority (53.1%) of cases had age &gt;50 years {41(51.25%)in controls and 44(55%) in cases}. Mean ages in two groups (controls and cases) were49.39+12.30 years and 53.31+10.09 years respectively (p = 0.029).44 (22.5%) patients weresmoker and 116 (72.5%) were non-smoker. In control group, 13 (16.3%) were smokers whereasin cases 31 (38.8%) were smokers (p = 0.002). Mean triglyceride levels were statistically highin atherosclerotic subjects as compared to healthy individuals (138.31+62.65 mg/dl and125.73+46.17 mg/dl respectively; p=0.024). Mean IMT of both right and left carotid arteriesexamined via carotid Doppler ultrasound were statistically high in atherosclerotic individuals ascompared to healthy subjects {(Right carotid; 0.83+0.23 mm and 0.63+0.16 mm respectively;p=0.022) (Left carotid; 0.85+0.25 mm and 0.64+0.16 mm respectively; p=0.004)}. MeanLeptin levels were statistically high in atherosclerotic individuals as compared to healthysubjects (332.32+555.31 ng/ml and 254.55+224.66 ng/ml respectively; p=0.001). However,the difference in Adiponectin levels was insignificant (7.93+4.80 μg/ml and 9.45+4.73 μg/mlrespectively; p=0.343). Conclusion: We conclude that plasma levels of Adiponectin and Leptinare significantly higher in atherosclerotic than normal individuals.

English

Objectives: To compare the adiponectin/leptin ratio and intima media thicknessbetween normal and atherosclerotic individuals. To find out the association of A/L ratio withIMT in atherosclerotic patients. Study Design: Comparative cross sectional study. Placeand Duration of Study: This study was conducted at department of Physiology, BMSI incollaboration with Radiology department, Jinnah Postgraduate Medical Centre Karachi, from16th December 2014 to 15th December 2015. Methodology: 160 subjects having Dopplerultrasound of neck were selected on the basis of pre-determined criteria. They were grouped incases i.e. atherosclerotic group (80 subjects) and control i.e. healthy group (80 subjects). Age&gt; 20 years, both genders, patients for ultrasound Doppler for neck region irrespective of theirprovisional diagnosis, patients suffering from co-morbid conditions like diabetes, hypertension,chronic kidney disease, chronic liver disease, asthma and COPD and Healthy individuals(Controls) were included in this study. Patients who fail to gave consent and patients whoseultrasound findings were not up to the mark required for diagnosis were excluded from thisstudy. Results: Out of 160 patients in this study in control Male: Female ratio was 1:1.1 whilein cases, Male: Female ratio = 1:1. Majority (53.1%) of cases had age &gt;50 years {41(51.25%)in controls and 44(55%) in cases}. Mean ages in two groups (controls and cases) were49.39+12.30 years and 53.31+10.09 years respectively (p = 0.029).44 (22.5%) patients weresmoker and 116 (72.5%) were non-smoker. In control group, 13 (16.3%) were smokers whereasin cases 31 (38.8%) were smokers (p = 0.002). Mean triglyceride levels were statistically highin atherosclerotic subjects as compared to healthy individuals (138.31+62.65 mg/dl and125.73+46.17 mg/dl respectively; p=0.024). Mean IMT of both right and left carotid arteriesexamined via carotid Doppler ultrasound were statistically high in atherosclerotic individuals ascompared to healthy subjects {(Right carotid; 0.83+0.23 mm and 0.63+0.16 mm respectively;p=0.022) (Left carotid; 0.85+0.25 mm and 0.64+0.16 mm respectively; p=0.004)}. MeanLeptin levels were statistically high in atherosclerotic individuals as compared to healthysubjects (332.32+555.31 ng/ml and 254.55+224.66 ng/ml respectively; p=0.001). However,the difference in Adiponectin levels was insignificant (7.93+4.80 μg/ml and 9.45+4.73 μg/mlrespectively; p=0.343). Conclusion: We conclude that plasma levels of Adiponectin and Leptinare significantly higher in atherosclerotic than normal individuals.

The Relationship Between Glycemic Variability and Inflammatory Markers in Obese Children with Insulin Resistance and Metabolic Syndrome.

Objective:Increased glycemic variability (GV) is associated with increased oxidative stress, vascular complications, and mortality in metabolic syndrome (MS) and diabetes mellitus patients. To investigate the relationship between GV and inflammatory parameters in obese children with insulin resistance (IR) and to elucidate their effects on the development of MS.Methods:Fifty obese adolescents with IR were included in the study. All patients underwent anthropometric measurements, body fat analysis, and continuous glucose monitoring system (CGMS) for 24 hours. Serum lipids, adiponectin, and interleukin-6 (IL-6) levels were measured. GV coefficient (GVC) was calculated using the standard deviation and the average glucose value obtained by CGMS. IR was diagnosed according to the results of oral glucose tolerance test (OGTT). MS was diagnosed according to the modified World Health Organization and the International Diabetes Federation criteria.Results:Twenty-seven of the patients had MS and the remaining had only IR. Body fat mass, HbA1c, IL-6 levels, and peak insulin levels in the OGTT were significantly higher in the group with MS, but there was no difference in adiponectin levels. GVC was not different between the groups, but GVC significantly positively correlated with homeostasis model of assessment for IR, as well as with fasting, peak, and total insulin levels when all the patients were analyzed, while no significant relation was detected with adiponectin and IL-6 levels.Conclusion:This study suggests that GV is not different among obese adolescents with IR and MS. There seems to be a significant association between GV and IR parameters. However, other diagnostic criteria of MS (hypertension and/or dyslipidemia) or elevated IL-6 levels does not cause further increase in GV.

Circulating Adiponectin Levels Differ Between Patients with Multiple Myeloma and its Precursor Disease.

ObjectiveAn increased risk of multiple myeloma (MM) has been observed among individuals with low pre-diagnostic circulating levels of adiponectin, a metabolic hormone that is typically underexpressed among those who are overweight or obese. To assess whether adiponectin may influence myeloma development or progression to frank MM, we compared circulating adiponectin levels across patients with different stages of MM and its precursor, monoclonal gammopathy of undetermined significance (MGUS).MethodsWe measured adiponectin in 213 patients with MGUS, smoldering MM, or fully developed MM. Differences in adiponectin levels across patient groups were assessed using multivariate linear regression.ResultsRelative to MGUS patients, adiponectin levels were statistically significantly lower among those with smoldering and fully developed MM, both overall (16–20% decrease; P=0.048) and among those with IgG/IgA isotypes (26–28% decrease; P=0.004). Among MGUS patients, adiponectin levels were significantly lower for those with the higher risk IgM isotype compared with those who had IgG/IgA isotypes (42% decrease; P=0.036).ConclusionsThe findings of this study, the largest to investigate adiponectin levels in patients with different stages of MM and the first to evaluate associations with clinical characteristics, suggest that reduced expression of adiponectin may be associated with progression from MGUS to MM.

Association of adiponectin, leptin and resistin with inflammatory markers and obesity in dementia

The aim of the study was to determine the role of adiponectin, leptin and resistin in various types of dementia and to investigate their association with inflammatory markers, insulin resistance and abdominal obesity. In 205 patients with dementia [89 with Alzheimer’s disease (AD), 47 with vascular dementia (VaD), 69 with mixed dementia (MD)], 113 persons with mild cognitive impairment and in 107 controls serum adiponectin, leptin and resistin levels, pro-inflammatory [interleukin-6 (IL-6), C-reactive protein (hsCRP) and chitotriosidase] and anti-inflammatory (25-OH vitamin D, HDL-cholesterol and paraoxonase 1) markers, as well as glucose metabolism parameters (glucose, insulin and HOMA-IR) were determined. In all-cause dementia adiponectin and resistin levels were significantly higher as compared to the controls; leptin levels did not show differences. Higher adiponectin levels concerned AD and MD, whereas higher resistin-VaD and MD. After stratification by abdominal obesity the differences in adiponectin levels remained significant in subjects without obesity. In all-cause dementia negative correlation of adiponectin with obesity, glucose metabolism parameters, IL-6 and hsCRP and positive correlation with HDL-cholesterol were found. Positive correlation of resistin with age, IL-6, hsCRP and chitotriosidase and negative correlation with HDL-cholesterol and paraoxonase 1 were stated. We conclude that dementia of neurodegenerative origin is characterized by elevated adiponectin levels, whereas dementia with vascular changes by increase of resistin. Association with inflammatory indicators may suggest the pro-inflammatory role of resistin in the development of dementia, especially in dementia of vascular mechanism. Identification of this novel biomarker may be important in preventing dementia.