Understanding the differences in respiratory functions and isometric strength between recreational athletes and non-athletes has a significant importance in the fields of Sports science, exercise physiology, and performance enhancement. The purpose of this study was to determine the respiratory functions and isometric strength differences among recreational athletes and non-athletes. A cross-sectional design was selected for this research with 16 recreational athletes and 16 non-athletes. Respiratory functions were assessed using spirometry tests, measuring parameters such as forced vital capacity (FVC), peak expiratory flow rate (PEFR), and forced expiratory volume in one second (FEV1). Isometric strength was measured by a handheld dynamometer for dominant and non-dominant hands. The results of the study showed a significant difference between recreational athletes and non-athletes for respiratory function parameters (FVC = t-3.00, p-.006 & FEV1= t-2.221, p-.011), whereas PEFR is insignificant (t-218, p-.219). The isometric strength is also significant between recreational athletes and non-athletes (Dominant arm = t—3.039, p-.005 & non-dominant arm= t-.255, p-.001). The study findings revealed significant differences in respiratory functions and isometric strength between recreational athletes and non-athletes, and there was a significant relationship between parameters. Athletes typically exhibit remarkable isometric strength due to their consistent engagement in activities designed to enhance muscle function. Future research should give priority to conducting long term studies that directly compare the effects of different sports disciplines on respiratory functions and isometric strength. Keywords: Isometric Strength, Pulmonary Functions, Forced Vital Capacity, Peak Expiratory Flow Rate, FEV1, Recreational Athletes.