Differences In Growth Rates Research Articles

Coral growth along a natural gradient of seawater temperature, pH, and oxygen in a nearshore seagrass bed on Dongsha Atoll, Taiwan.

Coral reefs are facing threats from a variety of global change stressors, including ocean warming, acidification, and deoxygenation. It has been hypothesized that growing corals near primary producers such as macroalgae or seagrass may help to ameliorate acidification and deoxygenation stress, however few studies have explored this effect in situ. Here, we investigated differences in coral growth rates across a natural gradient in seawater temperature, pH, and dissolved oxygen (DO) variability in a nearshore seagrass bed on Dongsha Atoll, Taiwan, South China Sea. We observed strong spatial gradients in temperature (5°C), pH (0.29 pH units), and DO (129 μmol O2 kg-1) across the 1-kilometer wide seagrass bed. Similarly, diel variability recorded by an autonomous sensor in the shallow seagrass measured diel ranges in temperature, pH, and DO of up to 2.6°C, 0.55, and 204 μmol O2 kg-1, respectively. Skeletal cores collected from 15 massive Porites corals growing in the seagrass bed at 4 sites revealed no significant differences in coral calcification rates between sites along the gradients. However, significant differences in skeletal extension rate and density suggest that the dynamic temperature, pH, and/or DO variability may have influenced these properties. The lack of differences in coral growth between sites may be because favorable calcification conditions during the day (high temperature, pH, and DO) were proportionally balanced by unfavorable conditions during the night (low temperature, pH, and DO). Alternatively, other factors were simply more important in controlling coral calcification and/or corals were acclimated to the prevailing conditions at each site.

Exploring reported population differences in Norway lobster (Nephrops norvegicus) in the Pomo Pits region of the Adriatic Sea using genome-wide markers.

The Norway lobster (Nephrops norvegicus) is one of the most important decapod crustacean seafood species in the Adriatic Sea. Previous research has identified significant differences in growth rates and maturation timing of Nephrops in the Pomo/Jabuka Pits area compared to other subpopulations in Adriatic fishing grounds. Here, we use 1,623 genome-wide single nucleotide polymorphisms (SNPs) to investigate whether the Pomo Pits subpopulation is genetically different from other sites in the Adriatic and neighbouring seas. We found no genetic differentiation among all sampled Adriatic sites, suggesting high gene flow between Pomo Pits Nephrops and those of surrounding areas. We also found genetic homogeneity between the Adriatic sites and single-site samples from the Aegean and Tyrrhenian Seas. However, we detected distinct genetic differentiation between all Mediterranean sites and an Atlantic site in western Scotland, which provides evidence for a phylogenetic break between the Atlantic and the Mediterranean. Our results indicate that Pomo Pits Nephrops are not genetically different from others sampled in the Adriatic and that key biological parameters in Pomo Pits Nephrops could be driven by spatial variation in fishing pressure and/or environmental factors rather than geographic isolation.

Comparative Transcriptomic Profiling of Brain and Liver in Phoenix Barbs (Spinibarbus denticulatus denticulatus) with Differential Growth Rates

Phoenix barb (Spinibarbus denticulatus denticulatus) is a notable fish species in South China and is valued for its ecological and economic importance. To elucidate the molecular basis underlying its growth, we collected transcriptome profiles from the brains and livers of individual fish with different growth rates and compared differentially expressed genes (DEGs) at 3 and 9 months after hatching (MAH). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the pathways associated with growth were predominantly enriched in fatty acid biosynthesis, AMPK signaling, PI3K-Akt signaling, estrogen signaling, and protein metabolism. Notably, a greater number of DEGs from the fast-growing group were associated with these pathways at the early growth stage (3 MAH) than at the later stage (9 MAH). Real-time quantitative PCR results further validated that the genes involved in these pathways exhibited higher expression levels in fast-growing samples. This study enhances our understanding of the genetic mechanisms underlying growth rate differences and provides valuable genetic resources for future growth-related molecular breeding programs of phoenix barbs.

Beyond the Surface: Interconnection of Viscosity, Crystal Growth, and Diffusion in Ge25Se75 Glass-Former.

The knowledge of viscosity behavior, crystal growth phenomenon, and diffusion is important in producing, processing, and practical applications of amorphous solids prepared in different forms (bulk glasses and thin films). This work uses microscopy to study volume crystal growth in Ge25Se75 bulk glasses and thermally evaporated thin films. The collected growth data measured over a wide temperature range show a significant increase in crystal growth rates in thin films. The crystal growth is analyzed using near-surface viscosities obtained in bulks and thin films using nanoindentation and melt viscosities measured by a pressure-assisted melt filling technique. The crystal growth analysis provides information on the size of the structural units incorporated into the growing crystals, essential for estimating the diffusion coefficients and explaining the difference in crystal growth rates in bulk and thin films. The crystal growth analysis also reveals the decoupling between diffusion and viscous flow described by the Stokes-Einstein-Eyring relation. Moreover, to the authors' best knowledge, the manuscript provides the first evaluation estimation of the effective self-diffusion coefficient directly from growth data in chalcogenide glass-formers. The present data show a similar relation between diffusion coefficients (D) and crystal growth rates (u): u ≈ D0.87, which is found in several molecular glasses.

Accounting for effects of growth rate when measuring ecological stability in response to pulse perturbations.

Ecological stability is a vital component of natural ecosystems that can inform effective conservation and ecosystem management. Furthermore, there is increasing interest in making comparisons of stability values across sites, systems and taxonomic groups, often using comparative synthetic approaches, such as meta-analysis. However, these synthetic approaches often compare/contrast systems where measures of stability mean very different things to the taxa involved. Here, we present results from theoretical models and empirical data to illustrate how differences in growth rates among taxa influence four widely used metrics of ecological stability of species abundances responding to pulse perturbations: resilience, recovery, resistance and temporal stability. We refer to these classic growth-rate-dependent metrics as 'realised' stability. We show that realised resilience and realised temporal stability vary as a function of organisms' growth rates; realised recovery depends on the relation between growth rate and sampling duration; and realised resistance depends on the relation between growth rate and sampling interval. To account for these influences, we introduce metrics intended to be more independent of growth rates, which we refer to as 'intrinsic' stability. Intrinsic stability can be used to summarise the overall effects of a disturbance, separately from internal recovery processes - thereby allowing more general comparisons of disturbances across organisms and contexts. We argue that joint consideration of both realised and intrinsic stability is important for future comparative studies.

Производительность труда в российской Арктике: структурная динамика и регионально-отраслевые тенденции

Labor productivity is a key indicator of economic development and public well-being. This study assesses the structural shifts, regional dynamics, and sectoral trends in the domain of labor productivity in the Russian Arctic from 2011 to 2021, identifying aggregated economic sectors. The findings reveal that labor productivity levels in the Russian Arctic exceed the national average, primarily due to the resource-based nature of regional economies. However, a major issue is the persistently high level of interregional differentiation in labor productivity within the Arctic. The study demonstrates that an initially high level of labor productivity does not necessarily ensure long-term growth. An analysis of structural shifts in labor productivity over the past decade indicates a shift towards the market sector of the Arctic economy, evidenced by an increased share in gross regional product (GRP) and a decrease in time worked. Within the market sector, several trends are observed: the leading role of the extractive industry is maintained due to its expanding share in GRP; the role of agriculture is growing, reflected in a decreased share of hours worked; and the importance of other market sector branches, particularly transport, communication, and electric power, is diminishing as their share of total hours worked increases. The observed decline in labor productivity within the non-market sector suggests the potential for negative social consequences for the Arctic population. An analysis of labor productivity growth rates indicates that the leading sectors in terms of growth are mining, finance and business services, construction, and trade. These sectors can be considered sources of economic growth in the Arctic. Interregional differences in labor productivity growth rates within the same sector of the Arctic economy suggest the influence of regional factors, highlighting the potential for discovering unique reserves of productivity growth in each region.

Verification of the kinetic electron role in the microinstabilities in a negative triangularity model equilibrium

Effect of kinetic electrons on negative triangularity plasmas has been investigated and compared against the corresponding positive triangularity plasmas, using the global gyrokinetic code X-point Gyrokinetic Code with scale-separated delta-f option without Coulomb collisions. Our model magnetic equilibria have strong positive and negative triangularities and weak magnetic shear. However, unusually large ρi/a and low density plasmas are chosen to maximize the nonlocal effect to investigate the finite ρi effect and to be clearly away from kinetic ballooning modes. Similar conclusions to previous flux tube and global simulations have been obtained in this highly nonlocal model plasma: it is essential to include kinetic electrons in the micro-instability study of negative triangularity plasmas. Most physics findings agree with existing reports, with some disagreement. We offer a new “effective trapping fraction” concept that can add to the explanation of the growth rate difference between NT and PT plasmas, pointing to the significant variation in trapped particle fractions that have turning points in the mode growth regions.

Larval Rearing of the Giant Trevally, Caranx ignobilis (Forsskål, 1775), Fed Live Food Combinations of Rotifers, Copepods and Artemia salina

The giant trevally, Caranx ignobilis, is a high value fish which has been studied for spawning in captivity and domestication in the Philippines in view of its aquaculture potential. Initial larval rearing of the species encountered mortality issues, and this study attempts to address the problem to improve their survival. Hatchery-bred C. ignobilis larvae were reared for 40 days in nine units of 100 L conical fiberglass tanks at a stocking density of 500 larvae.tank-1 under three feed treatments with different combinations of rotifers (Brachionus plicatilis), brine shrimp (Artemia) and copepods (Pseudodiaptomus spp.): T1- Brachionus plicatilis + Artemia, T2- Brachionus plicatilis + Pseudodiaptomus spp., and T3-Brachionus plicatilis + Pseudodiaptomus spp. + Artemia fed at 0800 h and 1600 h. The results showed that the inclusion of copepods in the feeding regimen had positive effects on survival. Survival rate was significantly higher (P < 0.05) in T2 (23.47 ± 3.18 %) than T1 (11.67 ± 1.90 %) but was not statistically different from T3 (14.37 ± 3.88 %). Length increment was significantly different (P < 0.05) among treatments but with no statistical difference in specific growth rate (P > 0.05). The erratic swimming behaviour leading to mortality associated with insufficient levels of highly unsaturated fatty acids in Artemia was not observed in T2, thus, validating the assumption that the feeding with copepods will increase survival during larval rearing. The use of copepods for C. ignobilis larviculture is seen to play an important role in the success of the seed production protocol for this species. However, a sustainable supply of copepods through appropriate mass propagation techniques must be ensured to achieve higher production of C. ignobilis seeds in the hatcheries.

Rapid Emergence of Resistance to Broad-Spectrum Direct Antimicrobial Activity of Avibactam.

Avibactam (AVI) is a diazabicyclooctane (DBO) β-lactamase inhibitor used clinically in combination with ceftazidime. At concentrations higher than those typically achieved in vivo , it also has broad-spectrum direct antibacterial activity against Enterobacterales strains, including metallo-β-lactamase-producing isolates, mediated by inhibition of penicillin-binding protein 2 (PBP2). This activity is mechanistically similar to that of more potent novel DBOs (zidebactam, nacubactam) in late clinical development. We found that resistance to AVI emerged readily, with a mutation frequency of 2×10 -6 to 8×10 -5 . Whole genome sequencing of resistant isolates revealed a heterogeneous mutational target that permitted bacterial survival and replication despite PBP2 inhibition, in line with prior studies of PBP2-targeting drugs. While such mutations are believed to act by upregulating the bacterial stringent response, we found a similarly high mutation frequency in bacteria deficient in components of the stringent response, although we observed a different set of mutations in these strains. Although avibactam-resistant strains had increased lag time, suggesting a fitness cost that might render them less problematic in clinical infections, there was no statistically significant difference in growth rates between susceptible and resistant strains. The finding of rapid emergence of resistance to avibactam as the result of a large mutational target has important implications for novel DBOs with potent direct antibacterial activity, which are being developed with the goal of expanding cell wall-active treatment options for multidrug-resistant gram-negative infections but may be vulnerable to treatment-emergent resistance.

Sex differences in human pre-gastrulation embryos.

Human fetuses exhibit notable sex differences in growth rate and response to the intrauterine environment, yet their origins and underlying mechanisms remain uncertain. Here, we conduct a detailed investigation of sex differences in human pre-gastrulation embryos. The lower methylation and incomplete inactivation of the X chromosome in females, as well as the sex-specific cell-cell communication patterns, contribute to sex-differential transcription. Male trophectoderm is more inclined toward syncytiotrophoblast differentiation and exhibits a stronger hormone secretion capacity, while female trophectoderm tends to retain cytotrophoblast program with stronger mitochondrial function as well as higher vasculogenesis and immunotolerance signals. Male primitive endoderm initiates the anterior visceral endoderm transcriptional program earlier than females. The cell cycle activities of the epiblast and primitive endoderm are higher in males compared to females, while the situation is opposite in the trophectoderm. In conclusion, our study provides in-depth insights into the sex differences in human pre-gastrulation embryos and contributes to unraveling the origins of the sex differences in human fetal development.

423 Comparative post-weaning performance characteristics of four breeds of rabbit in hot humid climate of Nigeria

Abstract Rabbits are one of the most popular livestock breeds raised for their meat, fur, and companionship. Rabbit meat is fast gaining prominence as a healthier meat compared with beef, mutton, pork and chevon, which are mostly consumed in Nigeria. This study investigated breed differences in mortality, survival rates and post-weaning growth rate of rabbits reared under inimical environmental conditions. The animals were fed commercially compounded feed with 18% crude protein and 2300Kcal metabolizable energy with intermittent feeding of Panicum sp and Tridax sp. The study area, located at latitude 6.5867° N and longitude 3.9700° E, had an average daily temperature of 35°C and relative humidity of 64%, which negatively impacted the adaptation, survival rates and growth performance of the rabbits. A total of 48 weaner rabbits 8 wk of age, comprising 12 each (9 does and 3 bucks) of Checkered, Chestnut, Dutch and Hyla breeds were reared for 10 wk post-weaning. Mortality was recorded across the breeds and the body weights (BW) of the rabbits were also recorded on a weekly basis for the entire 10-wk period. The study evaluated survival rates across breeds and sex and BW gain. Statistical analyses were conducted using the Minitab software for Chi Squared test of independence for counts and descriptive statistics and general linear model analysis of variance for bodyweight gain. The statistical model describing the analysis was Yijk = µ + αi + (α:β)ij + eijk where αi is the ith fixed effect of breed and βj is the jth fixed effect of sex. The model accounted for 67.03% variation in total weight gain. Further mean separation was done using the Tukey’s multiple comparison procedure. Mortality rates across breeds on the average was 56.25%, with Checkered, Chestnut, Dutch and Hyla respectively recording 66.67%, 41.67%, 50.00% and 66.67% mortality, respectively. Mortality rate across sexes was 63.89% for does and 33.33% for bucks; however, mortality rates were independent (P > 0.05) of both breed and sex with a Chi-Squared value of 2.285 and 3.41, respectively, despite the numerical differences in mortality rates across breeds and sexes. Average total BW gain during the study period across the breeds and within breeds is as presented in Table 1. The nesting of sex within breed revealed that the only significant (P > 0.05) difference was noticed between the Hyla buck and Chestnut doe, while other means were not statistically different (P > 0.05). The Chestnut breed with the least BW gain adapted well in the environment with greatest survival rate, while the Hyla breed had the greatest BW gain and least survival rate throughout the study. This study indicated a negative relationship between survival rates and BW gain, that lighter breeds of rabbit tend to have greater survival rates than the heavier breeds.

An Empirical Analysis of Growth Trends and Correlations among Life Funds, Pension & General Annuity Funds, and ULIP Funds in the Indian Life Insurance Industry

Purpose: This study aims to analyze the growth trends and correlations among three key fund types in the Indian life insurance industry: Life Funds, Pension & General Annuity Funds, and Unit-Linked Insurance Plans (ULIP) Funds. Explicitly, this study intends to answer the resulting research questions: • Which fund type has exhibited the highest AUM growth rate? • What are the key factors contributing to the growth of each fund type? • What are the implications of the findings for the Indian life insurance industry? Design/methodology/approach: The study employs a quantitative research methodology, focusing on historical data spanning from 2014 to 2023. By using statistical techniques such as Analysis of Variance (ANOVA) and correlation analysis, the research identifies and evaluates the significant differences and relationships among the various fund types. ANOVA is used to determine if there are statistically significant differences in growth rates among the funds, while correlation analysis helps in understanding the strength and direction of relationships between the funds. Findings: The analysis uncovers significant disparities in the growth rates of the different fund types. Specifically, Pension & General Annuity Funds demonstrate the highest growth rate among the three. Additionally, the study finds a weak positive correlation between Life Funds and ULIP Funds, indicating a slight tendency for these two fund types to grow together, although the relationship is not strong. Originality/value: This research contributes novel insights into the Indian life insurance industry by showcasing the distinct growth patterns and interrelationships among Life Funds, Pension & General Annuity Funds, and ULIP Funds. The findings are valuable for stakeholders in the industry, as they can inform strategic decisions related to investment, product development, and marketing. By understanding these trends and correlations, life insurance companies can better tailor their offerings to meet market demands and optimize their growth strategies. Key words: Life insurance industry, growth trends, correlations, fund types, investment decisions, product development, marketing strategies.

Effects of Harvest Time on Medicinal Qualities of Hemp

Harvest time impacts the physicochemical properties of hemp. This study investigated the relationship between harvest time on growth parameters, physiological parameter, and color, in addition to Cannabidiol (CBD), Tetrahydrocannabinol (THC) and Cannabigerol (CBG) concentrations. Siskiyou cannabis was harvested at 5 different stages after flowering 50% at week five to week nine. The rational explanation for stages on growth parameters, physiological parameters, color, and total physiochemical properties was found, while total color changes ranged from brightness (L*) decreased gradually from 66.06 at 5 weeks to a minimum of 16.14 at 9 weeks of flowering. On the contrary, the redness (a*) and yellowness (b*) increased from –0.65 and 8.95 at 5 weeks to their peak values of 19.99 and 34.24 at 9 weeks, respectively. The hue also decreased from 110.03 at 5 weeks to a minimum of 80.14 at 9 weeks, with samples being significantly (p <0.05) different. Tetrahydrocannabinol (THC) was higher than 1 % at week 5, which was lower than their Cannabidiol (CBD) concentrations, reaching 14.35% at week 8. Cannabigerol (CBG) in dried samples reached 2.01% at week 7. The average dry weight of inflorescence per plant peaked at 36.75 g in week 8 and week 9 respectively. Significant differences in Crop Growth Rate (CGR) were noted across the harvesting periods, notably 17.13 and 16.70 g cm⁻² day⁻¹ at weeks 8 and 9, respectively, representing the highest dry weight accumulation per unit area. This increase in dry weight accumulation indicates higher efficiency. Finally, the Harvest Index (HI) showed notable discrepancies among the post-flowering harvesting times, with the greatest total dry weight observed at 0.357 and 0.345 at weeks 8 and 9, respectively. These findings could be of industrial relevance for improving post-harvest processes while maintaining the quality of this regulated crop.

Genetic Sex and Origin Identification Suggests Differential Migration of Male and Female Atlantic Bluefin Tuna (Thunnus thynnus) in the Northeast Atlantic

ABSTRACTKnowledge about sex‐specific difference in life‐history traits—like growth, mortality, or behavior—is of key importance for management and conservation as these parameters are essential for predictive modeling of population sustainability. We applied a newly developed molecular sex identification method, in combination with a SNP (single nucleotide polymorphism) panel for inferring the population of origin, for more than 300 large Atlantic bluefin tuna (ABFT) collected over several years from newly reclaimed feeding grounds in the Northeast Atlantic. The vast majority (95%) of individuals were genetically assigned to the eastern Atlantic population, which migrates between spawning grounds in the Mediterranean and feeding grounds in the Northeast Atlantic. We found a consistent pattern of a male bias among the eastern Atlantic individuals, with a 4‐year mean of 63% males (59%–65%). Males were most prominent within the smallest (< 230 cm) and largest (> 250 cm) length classes, while the sex ratio was close to 1:1 for intermediate sizes (230–250 cm). The results from this new, widely applicable, and noninvasive approach suggests differential occupancy or migration timing of ABFT males and females, which cannot be explained alone by sex‐specific differences in growth. Our findings are corroborated by previous traditional studies of sex ratios in dead ABFT from the Atlantic, the Mediterranean, and the Gulf of Mexico. In concert with observed differences in growth and mortality rates between the sexes, these findings should be recognized in order to sustainably manage the resource, maintain productivity, and conserve diversity within the species.

Single-Molecule Fingerprinting Reveals Different Growth Mechanisms in Seed Amplification Assays for Different Polymorphs of α-Synuclein Fibrils.

α-Synuclein (αSyn) aggregates, detected in the biofluids of patients with Parkinson's disease (PD), have the ability to catalyze their own aggregation, leading to an increase in the number and size of aggregates. This self-templated amplification is used by newly developed assays to diagnose Parkinson's disease and turns the presence of αSyn aggregates into a biomarker of the disease. It has become evident that αSyn can form fibrils with slightly different structures, called "strains" or polymorphs, but little is known about their differential reactivity in diagnostic assays. Here, we compared the properties of two well-described αSyn polymorphs. Using single-molecule techniques, we observed that one of the polymorphs had an increased tendency to undergo secondary nucleation and we showed that this could explain the differences in reactivity observed in in vitro seed amplification assay and cellular assays. Simulations and high-resolution microscopy suggest that a 100-fold difference in the apparent rate of growth can be generated by a surprisingly low number of secondary nucleation "points" (1 every 2000 monomers added by elongation). When both strains are present in the same seeded reaction, secondary nucleation displaces proportions dramatically and causes a single strain to dominate the reaction as the major end product.

A preliminary study on the effects of substituting fishmeal with defatted black soldier fly (Hermetia illucens) larval meal on Asian seabass (Lates calcarifer) juveniles: Growth performance, feed efficiency, nutrient composition, disease resistance, and economic returns.

This study aims to develop an alternative aquafeed derived from insect meal for Lates calcarifer juveniles, with the objectives of exploring the physiological performance, biological parameters, and economic analysis of substituting fishmeal (FM) with defatted black soldier fly (Hermetia illucens) larvae (BSFL) as part of the diet of L. calcarifer juveniles. Five practical diets were formulated to include 0% (BSFL0, serves as control group), 5% (BSFL5), 10% (BSFL10), 15% (BSFL15), and 20% (BSFL20) of BSFL meal, partially or fully replacing FM, respectively. Each diet was randomly assigned to triplicate groups of 30 fish (10.70 ± 0.07 g) per tank (300 L). The fish were fed twice daily to apparent satiation. A 56-day feeding trial was conducted to evaluate the impacts of defatted BSFL meal replacing FM on the growth performance, feed efficiency, composition analysis of fish muscle, cumulative mortality rate challenged with Vibrio parahaemolyticus, and economic returns of L. calcarifer. These results show that differences in weight gain and specific growth rate among the different treatments were statistically significant (p < 0.05), except for the absence of significant variation (p < 0.05) between BSFL0 and BSFL5, and followed by BSFL10 > BSFL0 > BSFL5 > BSFL15 > BSFL20. However, the feed conversion ratio and protein efficiency ratio showed the opposite trend as above. Although the diets experienced a decline in crude protein content and an increase in crude fat content with the increasing proportion of BSFL substituting FM, the crude protein and fat content of fish muscle were only slightly influenced. It is worth mentioning that levels of nonessential amino acids, delicious amino acids, saturated fatty acids, omega-6, omega-9 in BSFL10 group all showed an increase compared with the control group. After a 7-day challenge test with V. parahaemolyticus, the cumulative mortality rates of the BSFL5 and BSFL10 groups, respectively, dropped to 5.20%, 5.28% compared to the control group's 16.88%; however, the mortality rates of BSFL15 (34.67%) and BSFL20 (41.77%) groups were found to be significantly (p < 0.05) increased. From an economic perspective, the incidence cost for each experimental group showed a trend as BSFL10 < BSFL0 < BSFL5 < BSFL15 < BSFL20, whereas the profit index in each treatment exhibited the opposite trend as above. It was concluded that low (5%) or moderate (10%) levels of BSFL substituting FM in aquaculture feed could improve the physiological performances, disease resistance, and economic returns of L. calcarifer. However, excessive substitution (>15%) leads to a negative effect. From an economic point of view, 10% inclusion of BSFL in practical diets is recommended for L. calcarifer juveniles.

Diverse polysaccharide production and biofilm formation abilities of clinical Klebsiella pneumoniae.

Klebsiella pneumoniae infections have become a growing threat for human health. The lack of understanding of the relationship between antibiotic resistance, mucoviscosity, and biofilm formation in clinical isolates impedes our abilities to effectively predict K. pneumoniae infection outcomes. These traits are also associated with fitness in natural populations and more specifically within a host. The Multidrug-Resistant Organism Repository and Surveillance Network offers a unique opportunity into the genetic and phenotypic variabilities in the K. pneumoniae isolates encountered in the clinics today. To this end, we compared the genetic profiles of these isolates with the phenotypic biofilm formation abilities, percent mucoviscosity, and growth rates. We found most isolates formed limited biofilm, although a select group of isolates could form extremely robust biofilms. Variation in biofilm formation could not be explained by difference in growth rate, suggesting specific genetic and physical determinants. Interestingly, the most mucoid strains in the populations were lacking the genetic element regulating the mucoid phenotype and three of these isolates were able to form robust biofilms. There was a significant phenotype-genotype correlation with decreased biofilm formation and an insertion sequence in the transcriptional activator of the type III fimbrial system. Finally, confocal microscopy highlighted the structural and spatial heterogeneity of biofilm among the most robust biofilm formers not detected by traditional methods. The combination of phenotypic, genomic and image analyses allowed us to reveal an unexpected phenotypic diversity and an intricate relation between growth, mucoviscosity and specific virulence-associated genetic determinants.

Developmental Changes of the Coronary Sinus Between the First and Second Trimesters: A Pilot Study.

To assess the coronary sinus (CS) presence, size, and CS to atrial ratio (CS/A) in the first trimester (FT) compared with the second trimester (ST). In this IRB-approved retrospective study, fetuses with adequate FT cardiac sweeps and normal ST hearts were included. Maternal and fetal characteristics were obtained. CS and atrial diameters were measured by a single sonologist. The CS/A ratio was compared between FT and ST. Linear regression assessed the relationship between biparietal diameter (BPD) and CS and atrial diameters. Statistical significance was set at P < .05. Among 99 fetuses, the CS was seen in 42/53 (79.2%) in the FT and 14/32 (43.8%) in the ST. No significant associations were found between CS visualization and the factors analyzed. The CS/A ratio was significantly higher in the FT versus ST (0.43 vs 0.25; P < .0001). Combined FT and ST data revealed positive correlations between BPD and both CS (slope = 0.018, P < .0001) and atrial diameters (slope = 0.135, P < .0001), suggesting differential growth rates, with the atrium exhibiting a faster growth rate as BPD increased. The CS appears prominent in the FT compared with the ST, likely due to differential growth rates between the CS and atrium. Remnants of embryonic structures, differences in myocardial drainage, and hemodynamics may also be contributing factors. Larger prospective studies are needed to confirm these findings and assess the value of the FT CS/A ratio.

Differential growth rate, water use efficiency and climate sensitivity between males and females of Ilex aquifolium in north-western Spain.

Dioecious plant species, i.e., those in which male and female functions are housed in different individuals, are particularly vulnerable to global environmental changes. For long-lived plant species, such as trees, long-term studies are imperative to understand how growth patterns and their sensitivity to climate variability differentially affect the sexes. Here, we explore long-term intersexual differences in wood traits, namely radial growth rates, water use efficiency quantified as stable carbon isotope abundance of wood cellulose, and their climate sensitivity in Ilex aquifolium trees growing in a natural population in NW Spain. We found that sex differences in secondary growth rates were variable over time, with males outperforming females in both radial growth rates and water use efficiency in recent decades. Summer water stress significantly reduced the growth of female trees in the following growing season, while the growth of male trees was primarily favoured by cloudy and rainy conditions the previous fall and winter combined with low cloud cover and warm conditions in summer. Sex-dependent lagged correlations between radial growth and water availability were found, with a strong association between tree growth and cumulative water availability in females at 30 months and in males at 10 months. Overall, our results point to greater vulnerability of female tress to increasing drought, which could lead to sex-ratio biases threatening population viability in the future.

Growth and allometric curves of southern brown howler monkey (Alouatta guariba): Insights on its ontogeny and conservation.

Ontogenetic sexual dimorphism is observed in different primate species, with ecological and evolutionary relationships explaining this pattern. Understanding the growth of the southern brown howler monkey elucidates not only the ecology and evolution but also contributes to conservation projects for this species. Throughout 20 years of the Centro de Pesquisas Biológicas de Indaial-Projeto Bugio, Brazil, we collected morphological data on 105 howlers of the Alouatta guariba species to identify the growth differences between ontogenetic categories and sexes and generate a growth curve to estimate the age of rescued individuals. Linear measurements were employed to obtain body length as well as the dimensions of the head and limbs. All individuals were also weighed to obtain body mass. We assessed growth rate and duration using allometric analysis based on the individuals' ages. We compared growth rate and duration among infant, juvenile, and adult howlers and between sexes. We provide growth curves for body size for both sexes using the Von Bertalanffy model. Infants have accelerated growth rate in comparison to the juveniles and adults, with no differences between sexes in establishing body length at this ontogenetic stage. Males have a prolonged development duration from the juvenile stage, reaching adulthood later than females, which explains the body length differences found in this species. Variables of head and limbs analyzed also showed differences in growth rate and duration, but not so consistently among ontogenetic stages. Mass was not a good variable to understand the growth differences of the animals, since many arrived feeble in the project and may have lost mass due to different circumstances in old age. Therefore, growth curves were obtained only for body length, allowing the estimation of the age of these animals when rescued from the wild to more effectively provide needed care in captivity.