Diet Of Common Carp Research Articles

Major diet of common carp (Cyprinus carpio L.) over different developmental stages in rice-field: agroecological interactions between fishes and rice in Sichuan, China, based on DNA metabarcoding approach

Major diet of common carp (Cyprinus carpio L.) over different developmental stages in rice-field: agroecological interactions between fishes and rice in Sichuan, China, based on DNA metabarcoding approach

Effect of Azolla meal-based diet on growth performance, haematological profile and proximate composition of common carp (Cyprinus carpio) fry

Optimising the cost of nutrients in fish diets is crucial for aquaculture production, as feed accounts for 50% of operational expenses in intensive aquaculture. The rising demand for mustard oil cake (MOC), a commonly used feed ingredient in Indian aquaculture, has led to a significant increase in its price due to its diverse applications. Azolla (Azolla pinnata), a free-floating freshwater plant, has attracted researchers in fish feed formulation due to its rich nutrient profile. The present study was conducted to evaluate the effects of replacement of MOC with Azolla meal in the diet of common carp (Cyprinus carpio) fry. Pelleted feed was formulated with Azolla meal replacing MOC at levels of 0, 10, 20, 30 and 40% and fed to fish. Growth performance, feed conversion ratio (FCR), haematological parameters and proximate composition in fish carcass were assessed after 90 days of experimental feeding. Simultaneously, a control feed without supplementing of Azolla meal was also prepared. Fish were fed daily with the experimental diets at 8, 6 and 4% of their body weight during the first, second and third months respectively, in the morning hours (10:00 hrs). Principal component analysis (PCA) was performed to assess the effect of formulated diets on fish carcasses composition and haematological parameters. The results showed that replacing 30% of MOC with Azolla meal in the diet, led to significantly (p≤0.05) higher specific growth rate (SGR) and percentage weight gain and significantly (p≤0.05) lower feed conversion ratio (FCR) at this concentration. Fish survival rate ranged from 80.00 to 93.33% among different treatments. Further, significant (p≤0.05) differences were observed in both haematological parameters of fish and the proximate composition of fish carcass. among the different treatments. These findings suggest that MOC can be effectively replaced with 30% Azolla meal in the diet for rearing common carp fry to fingerling stage, without compromising the growth performance. Keywords: Aquaculture, Feed conversion ratio, Health status, Mustard oil cake, Principal component analysis, Specific growth rate, Survival

Effect on growth, gonad development, health and disease resistance against Aeromonas hydrophila of Origanum minutiflorum essential oil in Cyprinus carpio

This study investigated the effects on growth performance, histopathology, intestinal histomorphology, antioxidant activity, serum biochemical parameters, and disease resistance against A. hydrophila of carp fed with oregano essential oil (EO). Fish (13.54±0.24 g) were fed with experimental diets containing 1.0 and 3.0 mL kg−1 oregano EO for 60 days. At the end of the experiment, no significant difference was found among the growth performances of fish fed with oregano EO supplemented diets (p>0.05). However, dietary administration of 3.0 mL kg−1 oregano EO had significantly increased survival rates of common carp post-challenged with A. hydrophila (p < 0.05). In addition, goblet cells number and villi length in the intestine were increased in fish fed with oregano EO diets (p < 0.001). Also, no pathological findings were observed in hepatopancreas, gonads and intestines in fish fed with oregano EO. Fish fed with a diet containing 3.0 mL kg−1 oregano EO showed higher catalase activity (CAT) than the other experimental groups (p < 0.05). Superoxide dismutase (SOD), melondialdehyde (MDA), total protein, albumin, AST, ALT and ALP levels were similar in oregano EO supplemented groups compared to the control (p > 0.05). Number of mature oocytes in female ovaries caused a slight increase in fish fed with oregano EO. Adding oregano EO to common carp diet showed improved intestinal histomorphology and increased the survival against the A. hydrophila. Therefore, O. minutiflorum EO can be used as a health promoter for carp.

FATTY ACID PROFILE OF COMMON CARP (CYPRINUS CARPIO) AFTER ADDITION OF ASTAXANTHIN TO THE FEED MIXTURE

Astaxanthin is a very strong antioxidant typically fed to rainbow trout mainly to alter the color of its’ meat to red. In our study, we incorporate a relatively low concentration of astaxanthin into the diet of common carp to enhance the chemical parameters and oxidation stability of carp meat without altering the typical coloration of meat. Also, we observed the fatty acid profile of reared fish. We observed an increase in protein content in animals with astaxanthin dietary addition. It was not statistically significant, but we observed a lowering of fat content in experimental groups. Regarding lipid oxidation, we did not observe any significant effect of astaxanthin on malondialdehyde production in samples. Fatty acid profiles were determined by gas chromatography and then compared. Only significant differences were observed in stearic and arachidonic acid and in the experimental group with lower astaxanthin addition. Our results conclude that dietary astaxanthin has no adverse effect on fatty acid profile or oxidation stability during storage (at -18 °C). On the other hand, astaxanthin has the potential to enhance the protein and lower the fat content, even in low concentrations.

Effect of Same Nutritional Components on the Enzyme Activity of Some Fungal Isolate (Aspergillus niger) in Common Carp Diets (Syprinus carpio L.)

Effect of Same Nutritional Components on the Enzyme Activity of Some Fungal Isolate (Aspergillus niger) in Common Carp Diets (Syprinus carpio L.)

Combined Effects of Butyl Hydroxytoluene and Vitamin C on the Growth Performance, Blood Biochemistry, and Antioxidative Status of Common Carp (Cyprinus Carpio)

Abstract Common carp are proper finfish species for intensification, though their feeds should be reinforced with functional additives to ensure high growth performance and health status. Thus, this study tested the combined effects of butyl hydroxytoluene (BHT) and vitamin C (VC) on the growth performance, blood biomarkers, and antioxidative capacity of common carp. Fish were allotted into four groups and fed on four diets where the control diet was free of VC or/and BHT, while the second and third diets contained dietary VC or BHT at 500 or 150 mg/kg, respectively. The fourth diet was supplemented with both VC (500 mg/kg) and BHT (150 mg/kg) (BHT+VC). After 70 days, feeding VC or/and BHT markedly affected the growth performance of common carp. Carps fed VC, BHT, or BHT+VC had higher (P&lt;0.05) FBW, WG, and SGR than fish fed the control diet, while the FCR was lower (P&lt;0.05) in fish fed both VC and BHT than the control. In addition, feeding BHT or both BHT+VC markedly (P&lt;0.05) reduced the AST, ALT, and urea. The blood protein, albumin, and globulin were higher (P&lt;0.05) in fish fed VC or/and BHT than in the control. Fish fed dietary VC, or BHT+VC, had lower (P&lt;0.05) total cholesterol (T-CHO) and triglycerides (TG) than those fed on the control or BHT. The SOD, CAT, and GPX were significantly higher (P&lt;0.05) in carp fed on dietary VC or/and BHT than in fish fed on the control diet. Nevertheless, the malondialdehyde level was lower (P&lt;0.05) in carp fed on VC or BHT+VC diets than fish on the control or BHT diets. The lysozyme activity was significantly higher (P&lt;0.05) in carp fed on the BHT+VC diet than in the control or VC. In conclusion, the study suggests that dietary BHT or/and VC can be added to the diets of common carp to enhance the growth performance, feed digestion, blood biomarkers, and antioxidative capacity.

Effects of solid-state fermentation product of yeast supplementation on liver and intestinal health, and resistance of common carp (Cyprinus carpio) against spring viraemia carp virus

This study aimed to investigate the effects of solid-state fermentation products of yeast (SFPY) on liver and intestinal health and disease resistance of common carp (Cyprinus carpio). A total of 200 common carp with an initial average weight of 2.55 ± 0.004 g were divided into 5 groups (4 replications per group and 10 fish per replication), and were fed with one of five diets, including a control diet and 4 diets supplemented with 2‰ (Y2), 3‰ (Y3), 4‰ (Y4), or 5‰ (Y5) SFPY, respectively, for 8 weeks. Results indicated that, the addition of SFPY to the diet of common carp did not affect the growth performance or survival rate of fish (P = 0.253). Interestingly, with the addition of SFPY, the triacylglycerol (TAG) content of the liver presented a linear decreasing tendency (P = 0.004), with significantly decreased in Y4 and Y5 groups (P = 0.035) compared with control. Serum lipopolysaccharide (LPS) content and diamine oxidase (DAO) activity presented a negative linear relationship with the addition of SFPY (P = 0.015, P = 0.030), while serum lipopolysaccharide binding protein (LBP) content first decreased and then increased (P < 0.001). The total antioxidant capacity (T-AOC) in the intestine of fish increased continuously with increasing SFPY supplementation (P = 0.026), reaching the highest level in Y5 group. The villus height in all experimental groups were significantly higher than that in the control group (P < 0.001). Furthermore, compared to the control, adding 3‰ SFPY to the control diet of common carp significantly increased the relative abundance of Fusobacteria (P = 0.018) and decreased that of Proteobacteria (P = 0.039) at phylum level, and increased the relative abundance of Cetobacterium (P= 0.018) and decreased that of Shewanella (P = 0.013) at genus level. Compared with the control, the relative mRNA expression level of spring viraemia of carp virus N protein (SVCV-n) in the kidney was lower than that of the control group without significance and bottomed out in Y4 group (P = 0.138). In conclusion, dietary SFPY enhanced the SVCV resistance capacity of common carp by improving liver and intestinal health and modulating the gut microbiota. Thus, SFPY is a potential feed additive to be used in aquaculture to reduce the huge economic loss of common carp due to SVCV disease. Based on liver TAG content and intestinal villus height, the optimal addition level of SFPY was 3.02‰ and 2.72‰, respectively.

Suitable Cottonseed Protein Concentrate Supplementation in Common Carp (Cyprinus carpio) Serves as an Effective Strategy for Fish Meal Sparing Based on Improvement in Intestinal Antioxidant Capacity, Barrier and Microbiota Composition.

The application of cottonseed protein concentrate (CPC) is an effective strategy to moderate the shortage of fish meal (FM) for the aquafeed industry. However, little attention has been paid to the effects of replacing fishmeal with CPC on cyprinid fish. This study used common carp (Cyprinus carpio) as the biological model and assessed the potential of applying CPC as a substitute for fishmeal in the diet of common carp. The proportion of fish meal substituted with CPC in the six diets was 0% (CPC0), 25% (CPC25), 50% (CPC50), 75% (CPC75), and 100% (CPC100). Each diet was fed to three replicate groups of common carp (4.17 ± 0.02 g) for 56 days. Results revealed that the CPC50 group significantly increased the growth indexes via up-regulating the genes of the GH/IGF axis and the TOR pathway. The intestinal digestive ability was also elevated in the CPC50 group via markedly increasing intestinal villus height, protease and lipase activities in the whole intestine, and the amylase activity of the foregut and midgut. The CPC50 group captured significantly higher activities and gene expressions of antioxidant enzymes and lower malonaldehyde contents via evoking the Nrf2/Keap1 signal pathway. The CPC50 group enhance the intestinal mechanical barrier via up-regulating the gene expressions of tight junction proteins and heighten the intestinal biological barrier by increasing the probiotics (Lactococcus) and decreasing the harmful bacteria (Enterococcus). But excessive substitution levels (75% and 100%) would compromise growth performance, intestinal antioxidant capacity, and immune function. The optimum substitution level was estimated to be 46.47%, 47.72%, and 46.43% using broken-line regression analyses based on mass gain rate, protein efficiency ratio, and feed conversion rate. Overall, the fishmeal in common carp feed could be substituted up to 50% by CPC without negative influence on growth, feed utilization, and or intestinal health.

Comparative evaluation of raw and fermented Eichhornia crassipes leaf meal in the diet of Cyprinus carpio fingerlings: Effects on growth performance, body composition and haemato-biochemical responses

Eichhornia crassipes leaf meal (ELM) was fermented by solid-state fermentation using Chaetomium globosum MTCC 4179 that resulted in considerable changes in its nutrients and anti-nutrient profile. Both fermented ELM (FELM) and raw ELM were evaluated in the feed of Cyprinus carpio fingerlings through a feeding trial of 56 days. Five iso-nitrogenous and iso-caloric diets viz. C, Control (30% DORB and 0% ELM or FELM), ELM15 (15% ELM with replacement of 50% DORB), ELM30 (30% ELM with replacement of 100% DORB), FELM15 (15% FELM with replacement of 50% DORB), and FELM30 (30% FELM with replacement of 100% DORB) were prepared and fed on satiation basis to 180 acclimated fish (body weight 8.38–8.48 g) of five respective groups in triplicate with the stocking density of 12 fish /tank. Significantly (P<0.05) higher weight gain (%), specific growth rate, and protein efficiency ratio and lower feed conversion ratio (FCR) were observed in ELM15 and FELM15 groups compared to the control. However, the growth performance of ELM30 and FELM30 groups showed no significant (P>0.05) difference with control group. Furthermore, fermented diets (FELM15 and FELM30) could not improve the growth performance of fish as compared to their non-fermented counterparts (ELM15 and ELM30). Total red blood cell count, packed cell volume (%), and total serum protein (g/dl) were significantly (P<0.05) higher in ELM15 compared to other ELM and FELM-based groups. Better body composition was observed in fish of ELM15 group, however, fish of control, and ELM30 showed similar body protein, lipid, ash and total carbohydrate. Thus, it is concluded that raw ELM can be incorporated in the diet of common carp up to the level of 30% with complete replacement of DORB without compromising the growth performance of fish. However, the fermentation of ELM with Chaetomium could not impart any additional benefits in terms of growth performance of fish. In addition, dietary inclusion of 15% raw or fermented ELM, in replacement of 50% DORB, can result in higher growth performance with better health condition of common carp fingerlings.

Effects of dietary supplementation of different ratios of koumine and gelsemin on the liver immunity of common carp (Cyprinus carpio, FFRC)

Gelsemium elegans Benth. (GEB) exhibits immunoregulatory and growth-promoting effects in animals. However, owing to its complex composition, the specific mechanism remains to be explored. Koumine (KM) and gelsemine (GS) are the two alkaloids with the highest content and strongest biological activity in GEB. In this experiment, four ratios (0,0 mg/kg, 2,2 mg/kg, 2,1 mg/kg, and 2:0.67 mg/kg) of KM: GS from natural GEB were incorporated into the diet of common carp (Cyprinus carpio, FFRC). A 10-week breeding experiment was conducted to explore the immune and metabolic effects of these different KM and GS ratios on the liver of C. carpio, analyzing histology, antioxidant enzyme activity, immune gene expression, and transcriptomics. Hepatocyte edema and apoptosis were observed in the treatment groups. Acid phosphatase activity in the treatment group was significantly higher than that in the control group (P < 0.05), and malondialdehyde activity was lower (P < 0.05) in the treatment group, demonstrating a dose effect of GS. Superoxide dismutase and total antioxidant capacity activities increased gradually with a decreasing GS ratio (P < 0.05). Compared to the control group, changes in the GS ratio regulated the expression of immune genes. The relative expression of CYP1B1, TNF-β, and Myd-88 increased with decreasing GS ratio in the combination. Conversely, the relative expression of CYP1A1, TNF-α, GPX, IGF1, GST, and TLR-2 decreased with decreasing GS ratio in the combination. A total of 81.33 GB of clean data, 1370 DEGs, and 9258 co-expressed genes were obtained from the transcriptome data. These coexpressed genes were grouped into 244 KEGG pathways. Non-alcoholic fatty liver disease, Fatty acid degradation, Oxidative phosphorylation, and the PPAR signaling pathway were among the most abundant signaling pathways identified. This information contributes to a better understanding of the underlying mechanisms of KM and GS on liver immunity and lays a theoretical foundation for their application.

Chlorogenic acid improves common carp (Cyprinus carpio) liver and intestinal health through Keap-1/Nrf2 and NF-κB signaling pathways: Growth performance, immune response and antioxidant capacity

In this experiment, we investigated the effects of adding chlorogenic acid (CGA) to the diet on growth performance, immune function, inflammation response, antioxidant capacity and its related mechanisms of common carp (Cyprinus carpio). A total of 600 fish were selected and randomly divided into five treatment groups and fed with CGA containing 0 mg/kg (CK), 100 mg/kg (L100), 200 mg/kg (L200), 400 mg/kg (L400) and 800 mg/kg (L800) for 56 days. The results of the experiment were as follows: addition of CGA significantly increased the WGR, SGR, FER, and PER of common carp (P < 0.05). The addition of 400–800 mg/kg of CGA significantly increased the serum levels of LZM, AKP activity, C3 and C4 concentration, and increased immune function of common carp (P < 0.05). Regarding antioxidant enzyme activities, adding CGA significantly increased SOD, CAT, and GsH-Px activities, while decreasing MDA content (P < 0.05). Compared with the CK group, the mRNA expression levels of NF-κB, TNF-α, and IL-1β were decreased. The IL-10 and TGF-β were increased in the liver and intestines of the CGA supplemented group. Meanwhile, the addition of CGA also significantly up-regulated the mRNA expression levels of Nrf2, HO-1, SOD, CAT, and GPX (P < 0.05). CGA also positively contributed to the development of the carp intestinal tract, as demonstrated by decreased serum levels of DAO, D-LA, and ET-1. And the mucosal fold height was increased significantly with increasing levels of CGA. In conclusion, the addition of CGA in the feed can enhance the growth performance, immune function and antioxidant capacity of common carp, and improve the health of the intestine and liver. According to the results of this experiment, the optimal addition amount in common carp diets was 400 mg/kg.

Protective Effects of Sanyar Prebiotic on Immunity and Tissue Changes of Common Carp (Cyprinus carpio) Exposed to CuSO4 Stress

Prebiotics, as feed additives, can activate the antioxidant and immune systems of fish and significantly improve the survival of aquaculture animals in stressful conditions. The exposure of fish to copper sulfate leads to oxidative stress, suppression of the immune system, and destruction of vital body tissues. This study aimed to investigate the protective effects of Sanyar prebiotic on the immunity and tissue changes of common carp (Cyprinus carpio) exposed to CuSO4 stress. Two hundred common carp fish with an average weight of 12.33 ± 0.93 g were stocked in four treatments (triplicates) for 60 days. The experimental treatments contained Sanyar commercial prebiotics in 100 g of food, which included P1 (1 ml prebiotic liquid), P2 (0.1 g prebiotic powder), P3 (0.5 ml liquid and 0.05 g powder), and treatment C (control treatment without any additives). At the end of the experimental period, the fish were exposed to acute toxicity of Cu (0.1 m/l) for 96 hours. Gill, kidney, and liver tissue samples were collected and evaluated after 96 hours. According to the results obtained, there were significant differences in specific growth rate (SGR) and final weight ( p &lt; 0.05). The lowest feed conversion ratio (FCR) was observed in the Sanyar treatments (1.56 ± 0.37), which had a significant difference from the control treatment (1.85 ± 0.35) ( p &lt; 0.05). The results showed that immunity indices such as lysozyme, cortisol, and ACH50 increased in the experimental treatments compared to the control treatment ( p &lt; 0.05). The highest and lowest lysozyme activities were observed in P2 (16.35 ± 0.57) and control treatments (13.15 ± 1.00), respectively ( p &lt; 0.05). Generally, no significant difference was observed between growth and nutrition indices in Sanyar treatments ( p &lt; 0.05). Symptoms of kidney, liver, and gill tissue damage were reported from mild (hyperemia and infiltration of inflammatory cells) to severe (hemorrhage and necrosis). The lowest severity of tissue damage was observed in the treatments fed with Sanyar food supplement. In general, the results of this study showed that the addition of Sanyar prebiotic to the diet of common carp improves the growth indicators and immune response and can also be beneficial in increasing fish resistance to copper toxicity.

Substituting Fishmeal and Fish by-Products (Internal Organs) for Imported Animal Protein

This study was conducted to investigate the effect of using different levels of dried carob powder combined with different proportions of dried fish offal (internal organs) as a substitute for animal protein in some growth parameters of common carp (Cyprinus carpio L.). The experiment took place at the fish hatchery of the Livestock Department in Swera, Wasit, Iraq. Eight circular containers were used in the experiment, consisting of 4 treatments with 2 replicates for each treatment. A total of 200 fingerlings weighing 52 grams each were randomly distributed among the containers, and the experiment lasted for 70 days. The results showed that treatment T2 outperformed the other treatments in terms of growth parameters. The total weight gain of fish in T2 exceeded that of the other treatments, recording the highest overall weight gain rate. Additionally, T2 had the highest relative and specific growth rates, indicating the fish’s utilization of the feed supplemented with 25% carob powder and 16.7% fish offal as a substitute for animal protein. Based on the study, it can be concluded that carob powder and fish offal (internal organs) can be used as alternatives to animal protein in the diets of common carp, serving as a source of animal protein. This contributes to improving the feed and protein quality, as evidenced by improvements in some growth parameters.

Synergistic Effect of Butyric and Propionic Supplementation in Common Carp Diets on Growth and Immunological Parameters

The experiment was conducted for the period from 1/9/2021 to 3/6/2022, at the first agricultural research and experiment station of the College of Agriculture, Al-Muthanna University, which is located north of the city of Samawah near the end of the Alatshan river, about 4 km, whose water was relied upon as a source in this study. The butyric and propionic acids were mixed in the following proportions, and were fed to the experimental fish by ten treatments, which was (P0.00%, B0.00%), the control treatment, (P0.05%B0.05%) the first treatment T1, (%P 0.05%, B 0.15%) second treatment T2, (%P 0.05 0.20 B%) the third treatment T3, (%P 0.15% B0.05%) the fourth treatment T4, ( %P 0.15 %B 0.15) the fifth transaction T5, (P 0.15%B 0.20%) was the sixth treatment T6, (%P 0.20%B 0.05) was the seventh treatment T7, (%P 0.20 %B 0.15) was the eighth treatment T8, (%P 0.20 %B 0.20) was The ninth treatment T9, and they were added to diets with a protein content of 29.14% and a total energy of 396.726 (Kcal/g), 180 common carp (Cyprinus carpio L) fish were distributed among the ten treatments, and each treatment had three replications in 30 plastic cages with diameters of 50 cm and a depth of 65 cm in a cylindrical shape, each cage has 6 fish. The results showed the superiority of T2 in all growth parameters FW, WG, DGR, RGR, SGR, MGR, FI, FCR, FCE, and PER, followed significantly by T1, then T3, then T4 in almost all growth parameters. From the foregoing, it can be said that butyric and propionic acids can be used as functional additives in common carp fish diets, as they improve metabolic growth coefficient, feed conversion efficiency, and increase food intake efficiency.

The Effect of Gum Arabic and Technomus as a Prebiotics on the Growth Parameters of Common Carp Cyprinus carpio L.

This study was conducted at the first agricultural research and experiment station, Agriculture college, Al-Muthanna university, for 85 days, including the localization period (for the period from 25/9/2022 to 20/12/2022), to determine the effect of adding gum Arabic and technomus as a prebiotic to the diet of common carp (Cyprinus carpio L.) on growth parameters. 162 common carp were used, with an average weight of 75±5 gm per fish, in 27 small tanks (baskets) of 0.107 cubic meters, they were randomly distributed to nine treatments with three replicates (6 fish for each replicate), were as followed: T1: (control treatment; without adding). T2: add 0% technomus with 0.5% gum Arabic to the diet. T3: add 0% technomus with 1.0% gum Arabic to the diet. T4: add 0.5% technomus with 0% gum Arabic to the diet. T5: add 0.5% technomus with 0.5% gum Arabic to the diet. T6: add 0.5% technomus with 1.0% gum Arabic to the diet. T7: add 1.0% technomus with 0% gum Arabic to the diet. T8: add 1.0% technomus with 0.5% gum Arabic to the diet. T9: add 1.0% technomus with 1.0% gum Arabic to the diet. The results showed a significant (P≤0.05) superiority of T8 compare with T1 and T2 treatments on weight gain, final weight, daily weight gain, relative growth rate, and specific growth rate. T7 was significantly superior to control treatment at the same traits.

Effect of varied amounts of ginger oil in the feed on common carp growth metrics L. Cyprinus Carpio

The current study was conducted in experimental cages in a mud pond, First Agricultural Research and Experiment Station, Agriculture College, Al-Muthanna University, to determine the effect of different levels of ginger oil on the diets of common carp Cyprinus carpio L., the experiment lasted for 82 days, including the 10-day acclimatization period. 80 common carp fish with an average weight of 77±0.56 g was used. It was randomly distributed to 5 treatments with 3 replicates (5 fish each replicate). The fish were fed on experimental diets, divided into four equal treatments in terms of protein percentages and different levels of ginger oil, and the percentage of seeds added to the treatments was 0, 0.25, 0.50 and 1.00%, respectively. The fish were fed on the experimental diets at 5% of the live weight, divided into 4 meals daily. The results indicated a significant superiority of all ginger oil treatments in the growth parameters of carp fish, represented by body weight, weight gain, and daily, specific and relative growth rate. Adding 0.50% ginger oil gave the best results, compared with all ginger oil treatments in growth parameters of carp fish. Keywords: ginger oil, growth parameters, common carp Cyprinus Carpio L.

Alleviative effect of poly-β-hydroxybutyrate on lipopolysaccharide-induced oxidative stress, inflammation and cell apoptosis in Cyprinus carpio

In this study, the alleviative effects of poly-β-hydroxybutyrate (PHB) in bioflocs on oxidative stress, inflammation and apoptosis of common carp (Cyprinus carpio) induced by lipopolysaccharide (LPS) were evaluated. Common carp were irregularity divided into 5 groups and fed five diets with 0 % (CK), 2 %, 4 %, 6 % and 8 % PHB. After 8-week feeding trial, LPS challenge was executed. Results showed that appropriate level of PHB enhanced serum immune function by reversing LPS-induced the decrease of C3, C4, IgM, AKP, ACP and LZM in serum, alleviated LPS-induced intestinal barrier dysfunction by decreasing the levels of 5-HT, D-LA, ET-1 and DAO in serum, increasing ZO-1, Occludin, Claudin-3 and Claudin-7 mRNA, improving intestinal morphology. Moreover, dietary PHB reversed LPS-induced the decrease of AST and ALT in hepatopancreas, while in serum exhibited the opposite trend. Suitable level of PHB reversed LPS-induced the reduction of GSH-PX, CAT, T-SOD and T-AOC in intestines and hepatopancreas, whereas MDA showed the opposite result. PHB alleviated LPS-induced the decrease of Nrf2, HO-1, CAT, SOD and GSH-PX mRNA, the increase of Keap1 mRNA. Appropriate level of PHB alleviated LPS-induced inflammation and apoptosis by up-regulating TGF-β, IL-10 and Bcl-2 mRNA, down-regulating NF-κB, TNF-α, IL-6, Bax, Caspase-3, Caspase-8 and Caspase-9 mRNA. Furthermore, PHB inhibited activation of NLRP3 inflammasomes by reducing the levels of NLRP3, Caspase-1, ASC, IL-1β and IL-18 mRNA and protein. In addition, the increases of dietary PHB linearly and quadratically affected LPS-induced adverse effects on common carp. Summary, this study suggested that appropriate level of dietary PHB alleviated LPS-induced oxidative stress, inflammation, apoptosis and the activation of NLRP3 inflammasome in common carp. And the appropriate level of PHB in common carp diets was 4 %.

Momordica charantia saponins administration in low-protein-high-carbohydrate diet improves growth, blood biochemical, intestinal health and microflora composition of juvenile common carp (Cyprinus carpio).

An 8-week feeding trial was conducted to explore the feasibility of Momordica charantia saponins (MCS) administration to facilitate the protein-sparing action of high carbohydrate in diets for juvenile common carp (Cyprinus carpio) with initial mass of 5.41±0.02g. Based on our previous study, four diets with different the ratio of protein and carbohydrate (P/C ratio) were designed: 32%P/40%C, 30%P/43%C, 28%P/46%C, 28%P/46%C supplemented with 0.16% MCS (28%P/46%C+MCS). Each diet treatment was divided into 3 replicates. Results revealed that 30%P/43%C group increased growth performance and intestinal digestion, decreased intestinal inflammation, and optimized the intestinal microbiota compared to 32%P/40%C group, which presented the stronger protein-sparing action of high carbohydrate. But if the P/C ratio reduced to 28%P/46%C or less, the saving action would be restrained. However, compared to the 30%P/43%C and 28%P/46%C groups, 28%P/46%C+MCS group significantly elevated growth performance and activities of digestive enzymes and antioxidative enzymes, whilst the opposite trend occurred in the contents of glucose, triglyceride, total cholesterol, low density lipoprotein cholesterol, blood urea nitrogen, glutamic oxalacetic transaminase, glutamic-pyruvic transaminase and malondialdehyde. In addition, 28%P/46%C+MCS group markedly upregulated the expressions of GH/IGF axis genes, genes involved in protein synthesis, antioxidant genes and anti-inflammatory cytokine, whilst the opposite trend occurred in the expressions of pro-inflammatory cytokines. Moreover, 28%P/46%C+MCS group obtained the remarkably higher Enterococcus proportion and lower Lactococcus proportion compared to the 30%P/43%C and 28%P/46%C groups, whereas the opposite occurred in 30%P/43%C group, which indicated that there existed differences in the improvement mechanism on intestinal microflora composition between MCS and appropriate P/C ratio. Combined with the above mentioned changes in our research, we concluded that 0.16% MCS administration in a 28%P/46%C diet could facilitate the protein-sparing action of high carbohydrate in diets for common carp, which could decrease the 5% dosage of soybean meal and synchronously reduce the 4% crude protein of diets without affecting the growth and immune ability for common carp.

Feasibility evaluation of fermented peanut meal to replace soybean meal in the diet of common carp (Cyprinus carpio): Growth performance, serum biochemistry, intestinal health and microflora composition

The effectiveness of soybean meal (SBM) substitution with fermented peanut meal (FPM) were appraised in common carp juveniles. A diet containing 30 % SBM was perceived as the control diet, then 25 %, 50 %, 75 % and 100 % of SBM was replaced by the FPM (FPM0, FPM25, FPM50, FPM75, FPM100 groups). Each group was fed to three replicate groups of common carp (4.83 ± 0.02 g) for 56 days. Results exhibited that FPM50 group attained the significantly higher mass gain rate and specific growth rate than those of the other groups except for FPM25, higher protein efficiency ratio and lower feed conversion rate than those of the FPM75 and FPM100 groups. The FPM50 group significantly increased the contents of serum total protein and albumin and decreased the content of serum urea nitrogen. The activity of hindgut protease was significantly enhanced in the FPM50 group. Significantly higher and lower intestinal villi height was, respectively, observed in the FPM50 and FPM100 group. The FPM50 group significantly enhanced the gene expression and activities of antioxidant enzymes in the intestine, reversely decreased the content of malonaldehyde. Significant increments in the occludin, myosin light chain kinase and claudin3 gene expressions in the FPM50 group improved the increase of intestinal permeability induced by pro-inflammatory factor related genes interleukin 1β, interleukin 6 and tumor necrosis factor α. Staphylococcus and Enterococcus in the intestine abundances were effectively suppressed in the FPM50 group, and Lactococcus became the dominant intestinal bacteria genera. The optimum substitution level was estimated to be 50.93 % and 49.89 % using broken-line regression analyses based on MGR and FCR. Overall, the soybean meal in common carp feed could be substituted up to 50 % by FBM without negative influence on growth, feed utilization and intestinal health.

The Effect of Phytase Addition in Promoting Partial Replacement of Soybean Meal with Sesame Seed Meal on the Hematological and Biochemical Criteria of Common Carp Fish Cyprinus carpio L.

The goal of this study is to study the effects of substitution of soybean meal by sesame seed meal in the diet of common carp, Cyprinus carpio L. Fish were fed seven experimental diets that included soybean meal substituted with sesame seed at 25, 35 and 45 percent, respectively, in the second, fourth and sixth diets and phytase (1200 IU\\ Kg) added with the same replacement in the third, fifth and seventh diets, as well as the control diet (first diet). The data analysis of the edible portion of the body demonstrated a significant superiority (P ≤ 0.05) in the dry matter and crude protein criterion for fish fed the phytase-containing ration over the control ration. The high ether extract and proportion of phosphorus deposited in the bodies of fish fed the fourth and seventh diets, but the percentage of ash did not differ significantly from the control diet. Fish fed the third diet had a significant increase in PCV, Hb and TP, whereas fish fed the seven diets had such a significant increase in the value of globulin when compared to the other experimental diets. When fish were fed the seventh diet compared to the control diet, albumin levels were significantly higher, while glucose levels were lower significantly in control compared with the rest of the experimental rations. However, there was no significant difference in cholesterol levels between the different experimental treatments. As fish were given the seventh diet, the percentage of triglycerides increased significantly and the ALT and AST criteria decreased significantly when compared to the control diet.