Ethylenediamine Research Articles

Ethylenediamine-modified activated carbon photocatalyst with the highest TiO2 attachment/dispersion for improved photodegradation of sulfamethazine

Due to the high pollution caused by several molecules, it is necessary to develop efficient materials to degrade these contaminants. Activated carbons (ACs) are exceptional and versatile adsorbents, and titanium dioxide (TiO2) has remarkable photocatalytic properties. The combination of activated carbon (AC) and TiO2 provides a system capable of simultaneously adsorbing and degrading several emerging contaminants. This study presents a new absorbent/photocatalyst material by modifying the AC with ethylenediamine (ET) and impregnated with TiO2 (AC-ET/TiO2). AC modified with amine groups keeps the TiO2 nanoparticles more bound onto its surface. A study exploring the dispersion onto the surface of unmodified AC revealed that the material lacked stability, releasing TiO2 particles into the solution. The characterization by nitrogen adsorption/desorption isotherms showed a mesoporous material that can have a synergic effect between the adsorption and photocatalysis. The photocatalyst was analyzed by field emission gun scanning electron microscopy (FEG-SEM) and demonstrated the presence of TiO2 dispersed on the surface. The AC-ET/TiO2 exhibited an improved photodegradation of sulfamethazine (SMT), superior to the commercial TiO2 – P25, by removing more than 90% of the SMT in 2 h of UV light irradiation. The reuse of the AC-ET/90TiO2 showed that this material is active after three photoreactions. In conclusion, amino groups insertion into AC is essential for the stability of the TiO2 on the surface. Besides, this material has increased photocatalytic activity with speedy degradation of SMT.

Comparative description of the extraordinary phenomenon "thermally activated isobaric partial structure compaction" of water as a solute in some alkanols and alkylamines

This review summarises the data available in the literature. It also includes the authors' published results of precision densimetric measurements. The research concerns with the physically unusual phenomenon of "thermally activated isobaric partial densification of the structure" (TIPCS) of dissolved water, or its so-called "negative partial molar expandability" (NPEA) in several organic solvents. They contain amphiproton hydroxyl-containing media of three alcohols: methyl alcohol (MA), tertiary butyl alcohol (TBAlcohol), and amyl or pentyl alcohol (TPA), so asprotophilic media of two amines: tert butylamine (TBAmine) and ethylenediamine (EDA). The discussed TIPCS phenomenon, associated with a decrease in the standard (partial at infinite dilution) volume of solvated water with increasing temperature, was discovered about half a century ago in alkanol solutions of H2O and recently - in water-containing media of alkylamines. However, nowadays this extraordinary effect has not yet found its physically based interpretation. It allows ones to predict the possibility of TIPCS occurrence in the binary liquid-phase system specifically selected for the study. Our comprehensive data analysis allowed us to make several inferences regarding the main characteristics of a standard solution of H2O in an organic solvent. They cause extraordinary changes in the volume of the formed solvatocomplex of water under the influence of increasing temperature. Firstly, the energy parameters of the intermolecular interaction (relative affinity) water solvent noticeably dominate over those of the solvent-solvent interaction. Those differences become more evident with increasing temperature. Secondly, a higher rate of thermal expansion of the organic solvent structure in volume (inbulk) is found than influence of temperature on structural packing of the resulting mixed molecular aggregate or water solvates complex. Thirdly, the difference in the parameters of water-solvent and solvent-solvent interactions depends not only on the proton-donor/acceptor properties of the molecules contacting in solution, but also on the configuration of the structural packing of the solvating medium. It determines the nature of steric hindrances to the formation of H-bonds. Therefore, the absolute values of the mentioned parameters of relative affinity at 298.15 K increase in the series: MA << EDA ≈ TBAmine < TPAlcohol < TBAmine. It can indicate a relative strengthening of the specific interaction (mainly through the formation of hydrogen bonds) between the molecules of water and amphiprotonic or protophilic solvent in the above sequence. Indeed, difference in the solvent-solvent and water-solvent hydrogen bonding energies in the discussed liquid media of alkylamines (TBAmine and EDA) and tertiary isomeric alkanols (TBAlcohol and TPA) - with the most evident basicity - turned out to be noticeably larger than in the structural packing of water methanol solution. The ability of the components to specific interactions is quite comparable in those compounds.

Сравнительная характеристика неординарного явления «термоактивируемого изобарического парциального уплотнения структуры» воды как растворенного вещества в некоторых алканолах и алкиламинах

This review summarises the data available in the literature. It also includes the authors' published results of precision densimetric measurements. The research concerns with the physically unusual phenomenon of "thermally activated isobaric partial densification of the structure" (TIPCS) of dissolved water, or its so-called "negative partial molar expandability" (NPEA) in several organic solvents. They contain amphiproton hydroxyl-containing media of three alcohols: methyl alcohol (MA), tertiary butyl alcohol (TBAlcohol), and amyl or pentyl alcohol (TPA), so asprotophilic media of two amines: tert butylamine (TBAmine) and ethylenediamine (EDA). The discussed TIPCS phenomenon, associated with a decrease in the standard (partial at infinite dilution) volume of solvated water with increasing temperature, was discovered about half a century ago in alkanol solutions of H2O and recently - in water-containing media of alkylamines. However, nowadays this extraordinary effect has not yet found its physically based interpretation. It allows ones to predict the possibility of TIPCS occurrence in the binary liquid-phase system specifically selected for the study. Our comprehensive data analysis allowed us to make several inferences regarding the main characteristics of a standard solution of H2O in an organic solvent. They cause extraordinary changes in the volume of the formed solvatocomplex of water under the influence of increasing temperature. Firstly, the energy parameters of the intermolecular interaction (relative affinity) water solvent noticeably dominate over those of the solvent-solvent interaction. Those differences become more evident with increasing temperature. Secondly, a higher rate of thermal expansion of the organic solvent structure in volume (inbulk) is found than influence of temperature on structural packing of the resulting mixed molecular aggregate or water solvates complex. Thirdly, the difference in the parameters of water-solvent and solvent-solvent interactions depends not only on the proton-donor/acceptor properties of the molecules contacting in solution, but also on the configuration of the structural packing of the solvating medium. It determines the nature of steric hindrances to the formation of H-bonds. Therefore, the absolute values of the mentioned parameters of relative affinity at 298.15 K increase in the series: MA << EDA ≈ TBAmine < TPAlcohol < TBAmine. It can indicate a relative strengthening of the specific interaction (mainly through the formation of hydrogen bonds) between the molecules of water and amphiprotonic or protophilic solvent in the above sequence. Indeed, difference in the solvent-solvent and water-solvent hydrogen bonding energies in the discussed liquid media of alkylamines (TBAmine and EDA) and tertiary isomeric alkanols (TBAlcohol and TPA) - with the most evident basicity - turned out to be noticeably larger than in the structural packing of water methanol solution. The ability of the components to specific interactions is quite comparable in those compounds.

A Novel Validated GC-MS/MS Method for the Estimation of N-Nitroso Dimethyl Amine and N-Nitroso Diethyl Amine in Zidovudine.

A novel method has been developed for the estimation of N-Nitroso dimethyl amine impurities (NDMA) and N-Nitroso diethyl amine (NDEA) in Zidovudine by using Gas chromatograph Triple Quadrupole Mass with Liquid autosampler (GC-MS/MS) and the method is validated as per International Conference on Harmonization recommendations. Sample analysis was executed for Zidovudine by developed method. Both NDMA and NDEA were detected in below quantitation limit for the Zidovudine batches. Efficient chromatographic separation was achieved on a DB-WAX 30m length × 0.25mm internal diameter, 0.5-μm film thickness, Triple quad-8040 GC-MS/MS. Quantification was carried out at Triple quad electron ionization source was at a column flow of 1.5mL/min at a column oven temperature 50°C. The precision was in the range of 0.9-2.5% for NDMA and 0.8-2.3% for NDEA, and regression analysis shows as r value (correlation coefficient) of is >0.99. This method is capable to detect the NDMA and NDEA impurities in Zidovudine at a level of 0.006ppm for limit of detection and 0.018ppm for limit of quantitation with respect to test concentration of 66.66mg/mL.

Development of Highly Sensitive Fluorescent Sensors for Separation-Free Detection and Quantitation Systems of Pepsin Enzyme Applying a Structure-Guided Approach.

Two fluorescent molecularly imprinted polymers (MIPs) were developed for pepsin enzyme utilising fluorescein and rhodamine b. The main difference between both dyes is the presence of two (diethylamino) groups in the structure of rhodamine b. Consequently, we wanted to investigate the effect of these functional groups on the selectivity and sensitivity of the resulting MIPs. Therefore, two silica-based MIPs for pepsin enzyme were developed using 3-aminopropyltriethoxysilane as a functional monomer and tetraethyl orthosilicate as a crosslinker to achieve a one-pot synthesis. Results of our study revealed that rhodamine b dyed MIPs (RMIPs) showed stronger binding, indicated by a higher binding capacity value of 256 mg g-1 compared to 217 mg g-1 for fluorescein dyed MIPs (FMIPs). Moreover, RMIPs showed superior sensitivity in the detection and quantitation of pepsin with a linear range from 0.28 to 42.85 µmol L-1 and a limit of detection (LOD) as low as 0.11 µmol L-1. In contrast, FMIPs covered a narrower range from 0.71 to 35.71 µmol L-1, and the LOD value reached 0.34 µmol L-1, which is three times less sensitive than RMIPs. Finally, the developed FMIPs and RMIPs were applied to a separation-free quantification system for pepsin in saliva samples without interference from any cross-reactors.

Fundamental properties and intermolecular interaction of triethylene glycol + ethylenediamine aqueous solution for CO2 capture

Solvent absorption as a significant method for carbon dioxide capture, exploring the mechanism of CO2 capture and regeneration solvents is essential for industrial applications. In this work, water (H2O) + (ethylenediamine (EDA) + triethylene glycol (TEG)) system with the constant mole ratio (TEG: EDA = 1: 1) was chosen as the liquid absorbent. In order to gain a deeper comprehension of solution properties of H2O + (EDA + TEG) system, the density (ρ) and viscosity (η) of fundamental properties were measured at P = 1005 hPa and T = (298.15 K to 318.15 K) with the temperature step of 5 K. The excess properties, including excess molar volume (VmE), viscosity deviation (Δη), and excess Gibbs energy of activation of viscous flow (ΔG*E) of H2O + (EDA + TEG) system were calculated from the experimental ρ and η values, and fitted well with the Redlich-Kister polynomial equation. The above results combined with the spectra proved the existence of intermolecular interaction in H2O + (EDA + TEG) system, which favorably fixed CO2. Subsequently, the aqueous solution with mass fraction of 15 wt% EDA and molar ratio EDA: TEG = 1: 1 was used for absorbing CO2 (room temperature) and desorbing (378.15 K) for 5-time cycles, and the cycling absorption performance decreased. So as to explore the reason of degradation, Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, and nuclear magnetic resonance carbon spectroscopy (13C NMR) were used to analyze the circulating absorption mechanism of CO2 in H2O + (EDA + TEG) system, which revealed that alkyl carbonates and partially indecomposable substance 2-imidazolidinones generated in the cyclic process, and provided some implications for the industrial application.

Properties of Thermoplastic High-ortho Phenolic Epoxy Fibers

The thermoplastic high-ortho phenolic resins (HPRs) were synthesized by a one-step synthesis using phenol, formaldehyde, zinc acetate, sulfuric acid, and small amounts of ethanol. It was then blended with epoxy resins, and a small amount of ethylenediamine (EDA) was added as a curing agent for dry spinning to produce the as-spun fibers. Heat curing and microwave curing treatments were performed to obtain phenolic epoxy fibers. Using Fourier transform infrared spectroscopy to study the structural changes during the fiber curing process revealed that the amino groups in EDA opened the epoxy groups to generate the hydroxyl groups. The hydroxyl groups can further react with the epoxy groups, which accelerate the curing reaction. Thermogravimetric analysis (TGA) results showed a higher residual carbon degree in the fibers after curing. Mechanical property testing demonstrated a significant improvement in the mechanical properties of the fibers. By modifying the blending and optimizing the curing process, the heat resistance of the fibers was successfully improved, resolving the issue of poor mechanical properties in the high-ortho phenolic epoxy fibers (HPEFs).

The Effect of Gypsum/Bassanite on the Retardation of Ethylene Diamine Tetra(Methylene Phosphonic Acid) Sodium in Oil Well Cement Slurry

Summary Organophosphonates are commonly used retarders to prolong the thickening time of oilwell cement slurry at medium and high temperatures. In this paper, the impact of calcium sulfate in cement on the retarding effect of ethylene diamine tetra(methylene phosphonic acid) sodium (EDTMPS) was explored. First, the thickening properties of cements from four different factories were studied in detail with varying additions of EDTMPS. The study revealed diverse thickening phenomena, including retarding, accelerating, and increasing the initial consistency of cement slurries. The heat flow of cement hydration was detected, and the mineral changes of cement slurries at the early stage (1–3 hours) were analyzed. Additionally, the effect of EDTMPS on the hydration of tricalcium aluminate (C3A) and gypsum (GP)/bassanite (BS) slurry was investigated through X-ray diffraction (XRD) and ion concentration test. Finally, two clinkers from the same cement factory were mixed with GP/BS of different dosages to study the effect of calcium sulfate type on the thickening properties of cement slurry with EDTMPS. The results revealed that EDTMPS slowed down the dissolution of GP while promoting the dissolution of C3A. The rapid hydration of C3A increased the consistency of cement slurry without the retarding effect of GP. However, EDTMPS promoted the dissolution of BS, which can retard the hydration of C3A. Therefore, EDTMPS is appropriate for cements containing BS.

Smartphone-Based Colorimetric Microfluidic Paper-Based Analytical Device for On-Site Detection of Calcium Ions in Milk Samples

Milk is a major dietary source of calcium; thus, accurate and precise quantification of calcium in milk and other dairy products is necessary for quality control. Conventional instrumental methods are sensitive and accurate but require expensive instrumentation, sample pretreatment, skilled personnel, and a long detection time. Herein, a microfluidic paper-based analytical device (µPAD) was developed to quantify calcium ions (Ca2+) in milk using a smartphone as a detector. The method utilized a complexometric reaction between Ca2+ and ethylene diamine tetraacetic acid with murexide as an indicator. The response was the average color intensity at the detection zones measured using the Color Picker application. The proposed µPAD exhibited a good linear detection range (1 to 10 mM), a low detection limit of 0.42 mM, and a short analysis time of 2 min. The determination of Ca2+ in milk samples using the proposed µPAD was consistent with a potentiometric method. The proposed µPAD demonstrates many advantages, including rapid detection, acceptable accuracy, low cost, low sample volume, and suitability for on-site quantification of Ca2+ in real samples.

Self-healing properties of GO nanofiltration membranes based on dialdehyde cellulose nanocrystals and ethylenediamine facilitate efficient separation of dyes

Graphene oxide (GO) membranes can purify wastewater due to their unique two-dimensional channels, but they are susceptible to mechanical damage during use, which reduces membrane performance. In this paper, EGO nanosheets were prepared by grafting ethylenediamine (EDA) small molecules onto GO at high temperatures and introducing aldehyde groups on the molecular chain of double aldehyde cellulose nanocrystals (DACNC), which reacted with reactive amino groups on the surface of the EGO nanosheets via a Schiff base reaction to create interlayer nanochannels in the composite membrane. The best performing EGO-DACNC24 composite membranes were prepared by adding different aldehyde substitution degrees and different amounts of DACNC to mediate the crosslinked interlayer nanochannels and network structure of the composite membranes. The water flux is more than 10 times that of pure GO membrane, and the rejection rate of dye molecules larger than 400 Da is always more than 99%. Excellent separation results are achieved after 120 h of operation due to the structural stability of the membrane resulting from chemical cross-linking. Damaged in practical applications, small amounts of added DACNC can achieve self-healing through the synergistic effect of dynamic Schiff base and hydrogen bonding interactions.

Detoxification of copper and zinc from anaerobic digestate effluent by indigenous bacteria: Mechanisms, pathways and metagenomic analysis

The presence of organic-complexed copper and zinc in anaerobic digestate effluent (ADE) poses persistent ecological toxicity. This study investigated the detoxification performance and biotic responses of indigenous bacteria against ethylene diamine tetraacetic acid (EDTA)-complexed Cu(II) and Zn(II). Heavy metals (HMs) stress induced reactive oxygen species (ROS) generation and enhanced extracellular polymeric substances (EPS) secretion. At a Cu(II) influent concentration of 20.0 mg·L−1, indigenous bacteria removed 88.2% of Cu(II) within nine days. The majority of copper and zinc sequestered by bacteria were stored in the cell envelope, with over 50% of copper and 60% of zinc being immobilized. Transmission electron microscopy mapping (TEM-mapping) revealed significant mineralization of copper and zinc on the cell wall. Proteins abundant in EPS, alongside humic acid-like substances, effectively adsorbed HMs. Indigenous bacteria exhibited the capacity to reduce cupric to the cuprous state and cupric is preferentially reduced to cuprous before reaching reducing capacity saturation. Sulfur precipitation emerges as a crucial pathway for Zn(II) removal. Metagenomic analysis indicated that indigenous bacteria upregulated genes related to HMs homeostasis, efflux, and DNA repair, enhancing its resistance to high concentrations HMs. This study provided theoretical guidance for employing bacterial consortia to eliminate HMs in complex aquatic environments.

Synthesis of Raspberry-like Nanoparticles via Surface Grafting of Positively Charged Polyelectrolyte Brushes: Colloidal Stability and Surface Properties.

A method to synthesize stable, raspberry-like nanoparticles (NPs), using surface grafting of poly(glycidyl methacrylate) (PGMA) brushes on a polystyrene (PS) core with varying grafting densities, is reported. A two-step functionalization reaction of PGMA epoxide groups comprising an amination step first using ethylene diamine and then followed by a quaternization using glycidyltrimethylammonium chloride generates permanently and positively charged polyelectrolyte brushes, which result in both steric and electrostatic stabilization. The dispersion stability of the brush-bearing NPs is dramatically improved compared to that of the pristine PS core in salt solutions at ambient (25 °C) and elevated temperatures (60 °C). Additionally, the grafted polyelectrolyte chains undergo a reversible swelling in the presence of different ionic strength (IS) salts, which modulate the surface properties, including roughness, stiffness, and adhesion. An atomic force microscope under both dry and wet conditions was used to image conformational changes of the polyelectrolyte chains during the swelling and deswelling transitions as well as to probe the nanomechanical properties by analyzing the corresponding force-sample separation curves. The quaternized polyelectrolyte brushes undergo a conformational transition from a collapsed state to a swelled state in the osmotic brush (OB) regime triggered by the osmotic gradient of mobile ions to the interior of the polymer chain. At IS ∼ 1 M, the brushes contract and the globules reform (salted brush state) as evidenced by an increase in the surface roughness and a reduction in the adhesion of the brushes. Beyond IS ∼ 1 M, quartz crystal microbalance with dissipation monitoring measurements show that salt uptake continues to take place predominantly on the exterior surface of the brush since salt adsorption is not accompanied by a size increase as measured by dynamic light scattering. The study adds new insights into our understanding of the behavior of NPs bearing salt-responsive polyelectrolyte brushes with adaptive swelling thresholds that can ultimately modulate surface properties.

Structure-Activity Relationship and Voltage Dependence for the Drug-Drug Interaction between Amiodarone Analogs and MNI-1 at the L-type Cav Channel.

The drug-drug interaction (DDI) between amiodarone (AMIO) and sofosbuvir (SOF), a direct-acting hepatitis-C NS5B nucleotide polymerase inhibitor, has been associated with severe bradyarrhythmia in patients. Recent cryo-EM data has revealed that this DDI occurs at the α-subunit of L-type Cav channels, with AMIO binding at the fenestration site and SOF [or MSD nucleotide inhibitor #1 (MNI-1): analog of SOF] binding at the central cavity of the conductance pathway. In this study, we investigated the DDI between 21 AMIO analogs, including dronedarone (DRON) and MNI-1 (or SOF) in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and hCav1.2 models. Our findings indicate that among the tested AMIO analogs in hiPSC-CMs at clinically relevant concentrations, only three analogs (AA-9, AA-10, and AA-17) were able to effectively substitute for AMIO in this DDI with 1 µM MNI-1. This highlights the importance of the diethyl amino group of AMIO for interacting with MNI-1. In the hCav1.2 model, desethylamiodarone (AA-12) demonstrated synergy with 90 µM MNI-1, while three other analogs with modifications to the position of the diethyl amino group or removal of iodo groups showed weaker synergy with 90 µM MNI-1. Interestingly, DRON did not exhibit any interaction with 270 µM SOF or 90 µM MNI-1, suggesting that it could safely replace AMIO in patients requiring SOF treatment, other clinically relevant differences considered. Overall, our functional data align with the cryo-EM data, highlighting that this DDI is dependent on the structure of AMIO and cardiomyocyte resting membrane potential. SIGNIFICANCE STATEMENT: Our findings point to specific residues in the AMIO molecule playing a critical role in the DDI between AMIO and MNI-1 (SOF analog), confirming cryo-EM results. Applied at clinically relevant AMIO's concentrations or projected MNI-1's concentrations at the resting potentials mimicking the sinoatrial node, this DDI significantly slowed down or completely inhibited the beating of hiPSC-CMs. Finally, these in vitro results support the safe replacement of AMIO (Cordarone) with DRON (Multaq) for patients requiring SOF treatment, other clinical caveats considered.

Electrophilicity Modulation for Sub-ppm Visualization and Discrimination of EDA.

Precise and timely recognition of hazardous chemical substances is of great significance for safeguarding human health, ecological environment, public security, etc., especially crucial for adopting appropriate disposition measures. Up to now, there remains a practical challenge to sensitively detect and differentiate organic amines with similar chemical structures with intuitive analysis outcomes. Here, a unique optical probe with two electrophilic recognition sites for rapid and ultra-sensitive ratiometric fluorescence detection of ethylenediamine (EDA) is presented, while producing distinct fluorescence signals to its structural analog. The probe exhibits ppb/nmol level sensitivity to liquidous and gaseous EDA, specific recognition toward EDA without disturbance to up to 28 potential interferents, as well as rapid fluorescence response within 0.2 s. By further combining the portable sensing chip with the convolutional algorithm endowed with image processing, this work cracked the problem of precisely discriminating the target and non-targets at extremely low concentrations.

Ethylenediamine modulate bonding interaction of solvation structure for wide-temperature aqueous ammonium-ion capacitor

Aqueous ammonium-ion capacitors (AAICs) are promising for large-scale energy storage owing to low cost and inherent safety, while their practical applications are suffered from performance under extreme environment. Low ion conductivity and high viscosity, as well as freezing of the electrolyte, are the main issues for the electrochemical performance failure at low temperatures. In this work, the AAICs were assembled with commercial carbon electrodes and antifreeze electrolyte, where the electrolyte with a freezing point lower than −115 °C is developed by using Ethylenediamine (EDA) as an additive with a volume ratio of 50 % to an aqueous solution of 0.5 M NH4Cl. This antifreeze electrolyte displays a superior ionic conductivity of 8.58 mS cm−1 and a weaker viscosity of 8.16 mPa s at low temperatures. Furthermore, the spectroscopic investigations and molecular dynamics (MD) simulations demonstrate that the addition of EDA can break the hydrogen bonds of water molecules and modulate the solvation structure. Therefore, the assembled AAICs with electrolytes of 0.5 M NH4Cl (50 %-EDA) could be operated at wide-temperature conditions steadily, exhibiting excellent capacity, rate performance and good cycling stability. This work provides a simple and effective strategy for wide-temperature energy storage devices.

Haematological parameters of sheep in the semi-arid zone of North Eastern Nigeria

Haematological parameters of sheep in the semi-arid zone of North-Eastern Nigeria were studied between August 2022 and April, 2023. Blood was sampled from one hundred and sixty-eight sheep comprising of Yankasa, Balami and Uda (56 per breed) at the Federal University Gashua Teaching and Research Farm, Gashua abattoir and Garin Alkali goats and sheep markets. The study was carried out to determine the blood profile of prominent indigenous sheep breeds in the study area. Blood samples were carefully collected from the animals into labeled sterile bottles containing Ethylene diamine tetra-acetic acid for haematological examination. Data analyzed for the effect of breed, sex and age were not significantly affected (p>0.05) for packed cell volume (PCV) and haemaglobin (Hb) concentration. Consequently, values of PCV and Hb differed significantly (p<0.05) with season in favour of wet season, (40.87%) and (13.63g/dl) for PCV and Hb respectively. Values for red blood cell (10.18 x103/µL), mean corpuscular hemoglobin concentration (35.11g/dl), mean corpuscular hemoglobin (12.71 Pg), mean corpuscular volume (45.86 Fl) and white blood cell (10.72 x103/µL) were significantly higher (p<0.05) for Uda sheep. Adult sheep generally had significantly (p<0.05) higher values for haematologic parameters considered. It was concluded that there were breed, sex, age and seasonal differences in haematological parameters of the sheep breeds studied. The blood parameters needed to be monitored frequently and appropriately controlled to ensure stability and adequate health and nutritional status of sheep.

Modulating surface functional groups in carbon dots for enhanced inhibition of β‐amyloid aggregation

AbstractGaining a profound understanding of carbon dots (CDs) structure and their potential to inhibit β‐amyloid (Aβ) aggregation holds significant importance in the quest for Alzheimer's disease (AD) prevention and treatment. Herein, CDs with varying structures were synthesized by adjusting the molar ratio of citric acid (CA) and ethylenediamine (EDA). Transmission electron microscopy (TEM) was used to characterize their size and morphology. Fourier transform infrared spectroscopy (FTIR) and X‐ray photoelectron spectroscopy (XPS) were applied to study their structure. Optical properties were analyzed through Ultraviolet (UV) spectroscopy absorption and fluorescence spectroscopy. The inhibitory effect of CDs on Aβ1–42 aggregation was explored using circular dichroism and atomic force microscopy (AFM). The results highlighted the substantial influence of the CA to EDA molar ratio on the surface structure of CDs, subsequently impacting their optical properties and efficacy in inhibiting Aβ1–42 aggregation. With increasing EDA content, CDs exhibited a rise in the number of amino functional groups while experiencing a reduction in carboxyl groups. CDs possessing more amino functional groups effectively induced alterations in the initial conformation of Aβ1–42, leading to a significant inhibition of Aβ1–42 aggregation.

Effective removal of Congo red from aqueous solutions using ethylenediamine-modified wheat straw as adsorbent

In this study, ethylenediamine (EDA)-modified wheat straw (EMWS) was prepared and used as an efficient adsorbent for removing Congo red (CR) from aqueous solutions. Adsorption of CR by EMWS was examined through static adsorption experiments to determine the effect of contact time, initial pH values, particle size of the adsorbent, solution concentration, and temperature. As pH ranged from 4.0 to 10.0, CR adsorption by the EMWS remained considerably elevated. The equilibrium adsorption capacity was determined to be 97.92 mg·g−1 for a CR concentration of 400 mg·L−1, pH of 4.0, temperature of 318 K, and EMWS dose of 2 g·L−1, which was found to be comparable to other materials used for CR adsorption. Following that, optimal conditions were used to conduct isothermal, kinetic, and thermodynamic studies. In the adsorption process of CR, the Freundlich isotherm model and the pseudo-second-order (PSO) kinetics provided the best fit, indicating the presence of both physisorption and chemisorption mechanisms. Electrostatic attraction and hydrogen bonding were primarily responsible for CR adsorption onto the EMWS. Based on thermodynamic investigations, the adsorption of CR on EMWS is determined to be spontaneous (ΔG < 0) and endothermic (ΔH = 60.83 kJ·mol−1), while also exhibiting an increase in disorder (ΔS = 289.9 J·mol−1·K−1). The elution was carried out using 0.20 M HCl, followed by washing the EMWS with water, resulting in no loss of functionality. This study suggests that EMWS possesses the potential to serve as a feasible and efficient adsorbent for CR removal in wastewater.

Covalently Immobilized EDTA onto Graphene Oxide Using Ethylene Diamine as a Crosslinker for Cu(II), Pb(II), and Zn(II) Sorption

ABSTRACT This study introduces a novel method of EDTA immobilization onto graphene oxide (GO) using ethylene diamine as a crosslinker for heavy metal removal. GO was prepared from graphite via a modified Hummer method. Ethylene diamine was used as a novel crosslinker to immobilize EDTA onto GO via amide coupling. GO was functionalized with ethylene diamine to produce GO-NH2 onto which EDTA (H-form) was covalently immobilized producing GO-EDTA. FTIR and TGA show successful covalent immobilization of EDTA. Pb(II), Cu(II), and Zn(II) were tested for sorption from aqueous solutions onto GO-EDTA (Na-form). Initial pH 6.0 was found optimum for metal sorption. The kinetic sorption data were found to fit the pseudo-second-order model well with better performance as the temperature rises. The activation energy (E a ) of metal sorption was between 10.6 and 32 kJ/mol indicating metal physisorption. Equilibrium sorption data were found to fit well the Langmuir model with higher uptake as the temperature rises. The sorption capacity was 72.4, 46.5, and 28.0 mg/g at 25°C while it was 86.2, 56.8, and 31.95 mg/g at 35°C for Pb(II), Cu(II), and Zn(II), respectively. Metal sorption follows the order of GO-EDTA > GO-NH2 > GO and follows the order of Pb(II) > Cu(II) > Zn(II) from the metal mixture. Metal sorption is endothermic and spontaneous. GO-EDTA shows efficient recycle properties for metal sorption.

Coupling interface constructions of ZnO@ZnS@FeOOH for photocatalytic hydrogen production performance

Photocatalysis is a reliable method to solve energy crisis and generate green energy hydrogen. ZnO@ZnS (ZOS) formed heterojunction repressed carrier complexation and thus enhanced photocatalytic performance, but its low photoresponse efficiency limited its photocatalytic performance. Therefore, in this paper, ZnO@ZnS was modified with ethylene diamine tetraacetic acid (EDTA) to form ZnO@ZnS@FeOOH composites with coupling interface. The modification of EDTA could inhibite the oxidation of FeOOH during the synthesis process, and what’s more it could enhance the charge transfer at the heterojunction interface to promote the separation of electron-hole pairs and photoresponsive ability to further enhanced the hydrogen precipitation performance. The concentration of EDTA and Fe sources were regulated to investigate the photocatalytic hydrogen production capacity of the composites. The results indicated that the ternary composite ZnO@ZnS@FeOOH had the superior hydrogen evolution performance with the hydrogen evolution rate of 529.17 umol g−1 h−1, which was 2.4 times higher than that of ZOS. EDTA acts as an electron channel to enhance the inter-interfacial electron transfer capability, and the loading of FeOOH formed a heterojunction structure, which promoted carrier separation and enhanced the photoresponse. It’s the synergistic effect to enhanced the photocatalytic hydrogen production performance. This paper provided a method for the construction of ternary heterojunctions and interfacial charge transport channels.