The Dietary Supplement Ingredient Database (DSID) is a federal initiative to provide analytically‐derived estimates of ingredients in dietary supplements because the actual content may differ from the labeled amount. Release 2 provides data for children's multivitamins (MVMs), updates adult MVM estimates and is available at http://dsid.usda.nih.gov. Representative children's MVMs were identified and purchased from multiple market channels. These supplements were analyzed for their vitamin and mineral content with certified reference materials. Mean percent differences from label were calculated for each nutrient and compared to labeled levels using regression analysis. The relationship between label and analytical content for each nutrient was identified as either linear (n=12) or quadratic (n=5). Mean % differences from label averaged 1 to <10% above label for eight nutrients (zinc, phosphorus, iron, magnesium, copper, vitamin B‐6, niacin, riboflavin); 10 to <20% above label for 5 nutrients (thiamin, folic acid, vitamin B‐12, manganese, and calcium); 20 to <30% above label for 3 nutrients (iodine and vitamins A and E) and >30% above label for vitamin D. Data estimates were linked to children's MVM products reported in the National Health and Examination Survey and can be used by researchers to more accurately quantify nutrient intake from dietary supplements. Funding: ARS/USDA & ODS/NIH Y1CN501006