Dietary Se Concentration Research Articles

49 Crossing-talk between micronutrient and macronutrient metabolism: Implication in health and disease

Abstract Recent goals of swine ration formulation have evolved from preventing nutritional deficiency diseases and maximizing growth performance to optimizing immune function and disease resistance with least climate impact. Thus, certain micronutrients such as selenium (Se) are widely considered to be health beneficial at dietary concentrations above nutrient requirements. During the past decade, we have conducted a number of experiments to determine impacts and mechanisms of dietary Se concentrations (0.03 to 1 or 3 mg/kg) on metabolism of carbohydrate, lipid, and protein in pigs from growing to finishing. Strikingly, pigs fed high-Se diets developed hyperinsulinemia and insulin resistance. Those pigs also had increased lipid, protein, saturated fatty acids (myristic, palmitic, stearic, and behenic acids), and aspartic acid, glutamine, and alanine and decreased polyunsaturated fatty acids (dihomo-γ-linolenic, oleic, α-linolenic, and eicosatrienoic acids) in the liver and(or) adipose tissues. The high-Se diets increased and decreased amounts of 5 and 2 selenoproteins, respectively, in tissues of pigs. These effects were associated with down-regulated insulin signaling (INSR and AKT), glycolysis (glucokinase) and gluconeogenesis (PEPCK) and up-regulated lipogenesis (FOXO1, SREBP1, ACC and FAS), and protein synthesis (mTOR and RPS6/S6). In contrast, high dietary fat intakes up- or down-regulated expressions of all selenoproteins except for TXNRD2 and TXNRD3 and showed a strong effect on expressions of DIO2, GPX6, and SELENOK. These induced selenogenome expression changes were associated with increased serum malondialdehyde and plasma concentrations of tumor necrosis factor alpha and interleukin-6, along with risk of obesity and chronic inflammation. Altogether, metabolisms of micronutrients and macronutrients in pigs are coordinated through interdependent and mutually regulated crossing talks at tissue, cellular, biochemical, and signal levels.

Evaluation of growth, antioxidant status, hepatic enzymes and immunity of Nanoselenium-Fed Cirrhinus mrigala.

This study was conducted to investigate the effects of selenium nanoparticle (Se-NP) supplementation on the growth performance, carcass composition, antioxidant status, hepatic enzyme activities, and immunity of Cirrhinus mrigala. For this purpose, fish with an average initial weight of 7.44 ± 0.04 g were fed five experimental diets containing 0 (control), 0.25, 0.5, 1, and 2 mg kg-1 Se-NPs diets for 90 days. The analysed selenium (Se) contents of the diets were 0.35, 0.64, 0.92, 1.43, and 2.39 mg kg-1. Twenty five fish were randomly distributed in each of 5 aquarium (36 × 23.7 × 24.3 inches) in triplicate. The results showed that supplementation with Se up to 0.92 mg/kg significantly increased (p<0.05) weight gain, weight gain% (WG%), and specific growth rate (SGR) by 34%, 33%, and 16%, respectively, compared to the control diet. Dietary Se concentrations up to 0.92 mg/kg significantly increased the crude protein and crude fat and reduced (p<0.05) the moisture content as compared to the control group. Fish fed 0.92 mg kg-1 Se had significantly lower malondialdehyde (MDA) contents and higher activities of catalase, superoxide dismutase, and glutathione peroxidase in liver and serum as compared to other experimental diets. Moreover, a significant increase (p<0.05) in the level of serum immunoglobulin and lysozyme (LYZ) activity was recorded in fish fed 0.92 mg/kg Se diet. Moreover, the highest (p<0.05) values of aspartate transaminase (AST) and alanine transaminase (ALT) were recorded in fish fed 2.39 mg/kg Se level. However, serum alkaline phosphatase (ALP) activity remained unaffected by dietary treatment. Broken-line regression analysis indicated that 0.83 mg/kg Se is required for the optimum growth performance of C. mrigala.

Maternal Transfer and Effects of Selenium on Early Life Stage Development of Redside Shiner (Richardsonius balteatus).

Maternal transfer of selenium (Se) to developing fish eggs during vitellogenesis can cause larval deformity and mortality. Previous studies have shown wide variation among fish species in both the magnitude of maternal transfer (exposure) and the egg Se concentration causing effects (sensitivity). We studied maternal transfer and effects of Se on early life stage development, survival, and growth of redside shiner (Richardsonius balteatus), a small-bodied cyprinid that has been reported to have relatively high ovary:muscle Se concentration ratios. Gametes were collected from lentic areas in southeast British Columbia (Canada) with a range of dietary Se concentrations related to weathering of waste rock from coal mining. Eggs were fertilized and reared in the laboratory from hatch to the onset of exogenous feeding. Larvae were assessed for survival, length, weight, Se-characteristic deformities, and edema. Eggs from a total of 56 females were collected, with egg Se concentrations from 0.7 to 28 mg/kg dry weight. Maternal transfer varied among sites, with egg:muscle Se concentration ratios ranging from <1 to >4. We also found that sampling residual ovaries can overestimate Se concentrations in ripe eggs by up to a factor of 5.7. A correlation between larval weight and egg Se concentration was identified, although the relationship was weak (r2 < 0.1) and appeared to be a site effect. No other relationships were observed between larval endpoints and egg Se concentrations up to the highest concentration tested, indicating that the effects threshold for this species may be >28 mg/kg dry weight in eggs. These data indicate that redside shiner is less sensitive to maternally transferred Se than most other tested fish species. Environ Toxicol Chem 2023;42:2350-2357. © 2023 SETAC.

Effects of Selenium Supplementation on the Ion Homeostasis in the Reproductive Organs and Eggs of Laying Hens Fed With the Diet Contaminated With Cadmium, Lead, Mercury, and Chromium

The objective of this study was to explore the toxic effects of different heavy metals in combination with their deposition and ion homeostasis in the reproductive organs and eggs of laying hens, as well as the alleviating action of selenized yeast. A total of 160 Lohmann pink-shell laying hens (63-week-old) were randomly allocated into four treatments with 10 replicates of four hens each. The four dietary treatments were the corn-soybean meal basal dietary (control; CON); the CON dietary supplemented with 0.4 mg/kg selenium from selenized yeast (Se); the CON dietary supplemented with 5 mg/kg Cd + 50 mg/kg Pb +3 mg/kg Hg + 5 mg/kg Cr (HEM), and the HEM dietary supplemented with 0.4 mg/kg selenium from selenized yeast (HEM+Se). The dietary HEM significantly increased Cd, Pb, and Hg deposition in the egg yolk and ovary, and Cd and Hg deposition in the oviduct and in the follicular wall (p < 0.05). The HEM elevated Fe concentration in the egg yolk, ovary, and oviduct (p < 0.05). The HEM decreased Mn concentration in the egg yolk, Fe, Mn, and Zn concentrations in the egg white, Cu concentration in the ovary, Mg concentration in the oviduct, as well as Ca, Cu, Zn, and Mg concentrations in the follicular walls (p < 0.05). Dietary Se addition elevated Se concentration in the egg yolk, oviduct, and follicular walls and Mg concentration (p < 0.05) in the oviduct, whereas it reduced Fe concentration in the oviduct compared with the HEM-treated hens. Some positive or negative correlations among these elements were observed. Canonical Correlation Analysis showed that the concentrations of Pb and Hg in the egg yolk were positively correlated with those in the ovary. The concentration of Cd in the egg white was positively correlated with that in the oviduct. In summary, dietary Cd, Pb, Hg, and Cr in combination caused ion loss and deposition of HEM in reproductive organs of laying hens. Dietary Se addition at 0.4 mg/kg from selenized yeast alleviated the negative effects of HEM on Fe and Mg ion disorder in the oviduct and follicle wall of hens.

Gpx4, Selenov, and Txnrd3 Are Three Most Testis-Abundant Selenogenes Resistant to Dietary Selenium Concentrations and Actively Expressed During Reproductive Ages in Rats.

Almost all selenogenes are expressed in the testis, and those have the highest and constant expressions will be the primary candidates for functional analysis of selenium (Se) in male reproduction. This study aimed to profile the mRNA expressions of the testis-abundant selenogenes of rat models in responses to growth and dietary Se concentrations. Forty-eight weaning SD male rats were fed Se deficient basal diet (BD) for 5weeks and then randomly grouped (n = 12/group) for being fed BD or BD plus 0.25, 3, or 5mg Se/kg for 4 more weeks before sacrifice. Abundances of selenogenomic mRNAs in the liver and testis were determined with relative qPCR and those of the testis-abundant selenogenes in 13 kinds of tissues were assayed with a molecular beacon-based qPCR. Spatiotemporal expressions of rat selenogenome were also analyzed with the RNA-Seq transcriptomic data published by NCBI. mRNA abundances of glutathione peroxidase 4 (Gpx4), nuclear Gpx4 (nGpx4), selenoprotein V (Selenov), and thioredoxin reductase 3 (Txnrd3) in the testis were significantly higher than that in any other tissues (P < 0.05). Moreover, testicular mRNA abundances of Gpx4, Selenov, and Txnrd3 were not affected by levels of dietary Se supplementation (P > 0.05), and much higher at 6-21weeks old than at 2 and 104weeks old (P < 0.05). The result showed that Gpx4, Selenov, and Txnrd3 were most highly expressed in the testis of rats especially at reproductive ages and resistant to the impact of dietary Se levels, which suggested their specific importance in male reproduction.

Comparison of selenomethionine and hydroxyselenomethionine on tissue selenium retention, and antioxidative capacity of giant grouper, Epinephelus lanceolatus , fed diet with soybean meal

The study aims to evaluate the effects of two organic selenium (Se) sources, selenomethionine (SeMet) and hydroxyselenomethionine (OH-SeMet), on tissue Se retention, and antioxidative capacity of giant grouper, Epinephelus lanceolatus, fed diets with soybean meal (SBM). The control diet containing 32.92% SBM (replaced 40% fish meal protein) was supplemented with 0.3, 0.6 and 1.0 mg Se/kg of SeMet and OH-SeMet. A reference diet with fish meal (FM) as main protein source was also included for comparison. The eight experimental diets were each fed to triplicate groups of grouper juveniles (initial weight: 25.99 ± 0.04 g) in a recirculation system with seawater for 8 weeks. The Se concentrations in whole body, muscle and liver were dependent on dietary Se concentrations, but not on its supplemental forms. Higher hepatic glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), but lower malondialdehyde (MDA) content, were observed in fish fed the reference diet than in fish fed the control diet. The results indicated that dietary FM protein replaced by SBM at 40% depressed growth performance, Se retention and antioxidative capacity of giant grouper. Both Se sources supplementation enhanced Se retention and antioxidative capacity, but not growth performance of the fish.

Dietary supplementation with increasing doses of an organic micromineral complex on juvenile Nile tilapia: Effects on the antioxidant defense system and tissue deposition

The effect of increasing amounts (0%, 25%, 50%, 75%, and 100%) of dietary supplementation with an organic micromineral complex (Fe, Zn, Cu, Mn, and Se) on antioxidant defenses and mineral deposition in tissues of Nile tilapia juveniles was evaluated, where 100% supplementation represented the average adopted by the feed industry in Brazil. Fish (initial weight 23.93 ± 0.80 g) were fed until apparent satiation twice a day for 56 days. The maximum deposition of Fe and Zn in the hepatopancreas occurred in fish given approximately 50% supplementation, whereas the deposition of Mn and Se increased linearly with the inclusion of the complex. The activity of catalase and superoxide dismutase in the hepatopancreas decreased in fish fed the 50% dose, when compared to those not receiving mineral supplementation or those receiving higher doses. Glutathione peroxidase (GPx) activity in the hepatopancreas increased as the dietary Se concentration increased. However, the concentration of metallothionein in the hepatopancreas showed an inverse relationship to the increase in dietary supplementation of the organic mineral complex. There was no relationship between the doses of organic micromineral supplementation and the activities of GPx, reduced glutathione, non-protein thiols, or protein carbonylation. However, diets supplemented with 50% to 100% promoted greater GPx activity when compared to the 0% supplemented diet. Supplementation with intermediate doses of organic microminerals, approximately 50% of that used in commercial tilapia diets, promoted the homeostasis of metal metabolism, especially for Fe and Zn.

Lethal impacts of selenium counterbalance the potential reduction in mercury bioaccumulation for freshwater organisms

Mercury (Hg), a potent neurotoxic element, can biomagnify through food webs once converted into methylmercury (MeHg). Some studies have found that selenium (Se) exposure may reduce MeHg bioaccumulation and toxicity, though this pattern is not universal. Se itself can also be toxic at elevated levels. We experimentally manipulated the relative concentrations of dietary MeHg and Se (as selenomethionine [SeMet]) for an aquatic grazer (the mayfly, Neocloeon triangulifer) and its food source (diatoms). Under low MeHg treatment (0.2 ng/L), diatoms exhibited a quadratic pattern, with decreasing diatom MeHg concentration up to 2.0 μg Se/L and increasing MeHg accumulation at higher SeMet concentrations. Under high MeHg treatment (2 ng/L), SeMet concentrations had no effect on diatom MeHg concentrations. Mayfly MeHg concentrations and biomagnification factors (concentration of MeHg in mayflies: concentration of MeHg in diatoms) declined with SeMet addition only in the high MeHg treatment. Mayfly MeHg biomagnification factors decreased from 5.3 to 3.3 in the high MeHg treatment, while the biomagnification factor was constant with an average of 4.9 in the low MeHg treatment. The benefit of reduced MeHg biomagnification was offset by non-lethal effects and high mortality associated with ‘protective’ levels of SeMet exposure. Mayfly larvae escape behavior (i.e., startle response) was greatly reduced at early exposure days. Larvae took nearly twice as long to metamorphose to adults at high Se concentrations. The minimum number of days to mayfly emergence did not differ by SeMet exposure, with an average of 13 days. We measured an LC50SeMet for mayflies of 3.9 μg Se/L, with complete mortality at concentrations ≥6.0 μg Se/L. High reproductive mortality occurred at elevated SeMet exposures, with only 0–18% emergence at ≥4.12 μg Se/L. Collectively, our results suggest that while there is some evidence that Se can reduce MeHg accumulation at the base of the food web at specific exposure levels of SeMet and MeHg, Se is also toxic to mayflies and could lead to negative effects that extend across ecosystem boundaries.

The effects of dietary Se on productive and reproductive performance, tibial quality, and antioxidant capacity in laying duck breeders

This study evaluated the optimal concentrations of dietary Se for the productive and reproductive performance, tibial quality, and antioxidant status in duck breeders aged 23 to 49 wk. In total, 432 Longyan duck breeders aged 22 wk were allotted randomly to 6 treatments, each with 6 replicates of 12 individually caged birds. The experiment lasted for 27 wk, and birds were fed corn-soybean meal-based diets containing 0.11, 0.19, 0.27, 0.35, 0.43, or 0.51 mg Se/kg, respectively. The tested dietary Se levels did not affect egg production and tibial quality of duck breeders. The Se contents of the shell, yolk or albumin, whole egg, and the fertility of set eggs increased in a linear and quadratic manner (P < 0.05) in response to the increased dietary Se level, whereas the yolk malondialdehyde (MDA) and embryonic mortality decreased. The activities of glutathione peroxidase 3 (Gpx3) in plasma and Gpx1 in the erythrocytes and livers of breeder ducks increased in a linear and quadratic manner (P < 0.05) in response to increased dietary Se levels, whereas the total superoxide dismutase (T-SOD) activity increased and the MDA concentration decreased in the liver. The activity of Gpx3 in the plasma and Gpx1 in the erythrocytes and livers of newly hatched ducklings increased linearly (P < 0.01) with the increase in Se level, whereas the T-SOD activity and MDA concentration did not change. In conclusion, diets containing 0.27 mg Se/kg led to the highest egg fertility and hatchability in Longyan duck breeders, and using levels >0.19 mg Se/kg diet enhanced the antioxidant capacity in breeders and their offspring. The regression model indicated that dietary Se levels 0.19, 0.27, 0.28, 0.24, and 0.30 mg/kg are optimal levels to obtain maximum Se deposition efficiency in eggs, egg fertility, Gpx1 activity in erythrocytes and liver in duck breeders, and plasma activity of Gpx3 in newly hatched ducklings, respectively.

Dietary selenium required to achieve body homeostasis and attenuate pro-inflammatory responses in Atlantic salmon post-smolt exceeds the present EU legal limit

Selenium (Se) supplementation either as inorganic or organic form was evaluated in Atlantic salmon post-smolt in vivo and in vitro. The basal diet was formulated to be low in fish meal and contain 0.24 mg Se kg−1; six other diets with Se inclusion of 0.15, 0.4, 0.7 or 1.1 mg kg−1 as sodium selenite (SS) and 0.15 or 0.4 mg kg−1 as L-selenomethionine (SM) were formulated from the basal diet. The diets were fed to Atlantic salmon post-smolt (mean initial weight, 216 ± 27 g) in triplicate groups (35 fish tank−1) and reared in flow-through seawater (33 ppt) at 10–12 °C for 9 weeks. At the end of the feeding trial, whole fish and tissues were sampled for in vivo assessment; whereas, liver cells and head kidney leukocytes (HKL) were isolated and their primary cultures used for in vitro assessment following exposure to hydrogen peroxide (H2O2), lipopolysaccharide (LPS) or poly I:C (PIC). Growth, feed intake, feed conversion ratio, specific growth rate, hepato-somatic index, proximate composition and mineral concentration of the whole fish (except for Se) were unaffected by dietary Se (p > .05). Hematocrit was significantly higher in fish fed the 0.4 mg Se supplemented feeds, irrespective of the Se source (p = .02). The Se concentration in whole body, liver, muscle, plasma, kidney and liver/kidney Se ratio increased with increasing dietary Se concentration (p < .0001). Level of oxidised glutathione (GSSG) in liver and head kidney followed a quadratic function (p < .05) indicating that the concentrations were lower at intermediate SS supplementation of 0.4 and 0.65 mg Se kg−1 (total Se, 0.65 and 0.87 mg Se kg−1). Impact of Se sources on glutathione redox status was similar. Slope-ratio analysis revealed SM to be more efficient than SS in improving apparent availability, whole body or tissues Se status, Se retention and reducing Se loss to the environment. In vitro, the mRNA expression of p38mapk and aif, in liver cells were affected by the impact of dietary Se, but not by the treatment of H2O2 (p < .05). In the HKL, the LPS and PIC induced pro-inflamatory action of il-1β, cox2, nfkβ and viperin were attenuated by SM supplementation, but not by SS (p < .001). Dietary Se supplementation required to the basal diet containing 0.24 mg Se kg−1 was 0.41 mg Se as SS (total 0.65 mg kg−1) or 0.17 mg Se as SM (total 0.41 mg kg−1) based on body Se homeostasis or tissue Se status. SM inclusion at 0.4 mg kg−1 diet (total, 0.65 mg kg−1) attenuated LPS or PIC induced pro-inflammatory responses in vitro. Overall, Se requirement of Atlantic salmon post smolt was 0.27 mg kg−1 diet, on availale basis. Dietary Se level required to maintain body Se homeostasis and improved health status of Atlantic salmon fed plant-based diets (0.65 mg kg−1 diet) exceed the existing EU maximum limit of 0.5 mg Se kg−1 diet. SM as the Se source in salmon feeds has the potential to improve salmon health and reduce Se emissions from Norwegan salmon farming by 60 to 70%.

Effects of sodium selenite and coated sodium selenite on lactation performance, total tract nutrient digestion and rumen fermentation in Holstein dairy cows

Se can enhance lactation performance by improving nutrient utilization and antioxidant status. However, sodium selenite (SS) can be reduced to non-absorbable elemental Se in the rumen, thereby reducing the intestinal availability of Se. The study investigated the impacts of SS and coated SS (CSS) supplementation on lactation performance, nutrient digestibility, ruminal fermentation and microbiota in dairy cows. Sixty multiparous Holstein dairy cows were blocked by parity, daily milk yield and days in milk and randomly assigned to five treatments: control, SS addition (0.3 mg Se/kg DM as SS addition) or CSS addition (0.1, 0.2 and 0.3 mg Se/kg DM as CSS addition for low CSS (LCSS), medium CSS (MCSS) and high CSS (HCSS), respectively). Experiment period was 110 days with 20 days of adaptation and 90 days of sample collection. Dry matter intake was higher for MCSS and HCSS compared with control. Yields of milk, milk fat and milk protein and feed efficiency were higher for MCSS and HCSS than for control, SS and LCSS. Digestibility of DM and organic matter was highest for CSS addition, followed by SS addition and then control. Digestibility of CP was higher for MCSS and HCSS than for control, SS and LCSS. Higher digestibility of ether extract, NDF and ADF was observed for SS or CSS addition. Ruminal pH decreased with dietary Se addition. Acetate to propionate ratio and ammonia N were lower, and total volatile fatty acids (VFAs) concentration was greater for SS, MCSS and HCSS than control. Ruminal H ion concentration was highest for MCSS and HCSS and lowest for control. Activities of cellobiase, carboxymethyl-cellulase, xylanase and protease and copies of total bacteria, fungi, Ruminococcus flavefaciens, Fibrobacter succinogenes and Ruminococcus amylophilus increased with SS or CSS addition. Activity of α-amylase, copies of protozoa, Ruminococcus albus and Butyrivibrio fibrisolvens and serum glucose, total protein, albumin and glutathione peroxidase were higher for SS, MCSS and HCSS than for control and LCSS. Dietary SS or CSS supplementation elevated blood Se concentration and total antioxidant capacity activity. The data implied that milk yield was elevated due to the increase in total tract nutrient digestibility, total VFA concentration and microorganism population with 0.2 or 0.3 mg Se/kg DM from CSS supplementation in dairy cows. Compared with SS, HCSS addition was more efficient in promoting lactation performance of dairy cows.

Effect of sub-chronic dietary L-selenomethionine exposure on reproductive performance of Red Swamp Crayfish, (Procambarus clarkii)

The effect of selenium (Se) on the reproductive system has been investigated in both humans and vertebrates, but few studies of female fertility and reproduction in invertebrate have been reported. This study is aimed to investigate the effect of SeMet on growth performance and reproductive system after crayfish were fed with graded levels of dietary SeMet (0, 1.49, 3.29, 10.02, 30.27 or 59.8 μg Se/g dry weight) for 60 days. Crayfish treated with the high levels of SeMet (10.02, 30.27 and 59.76 μg Se/g) exhibited decreasing FW and CL in both male and female. Interestingly, Se accumulation was higher in ovary than in other tissues, suggesting that ovary may serve as a target organ for Se accumulation. We found that dietary Se concentration of 10.02 μg Se/g significantly improved the spawning rate, promoted the synchronized spawning, and up-regulated the expressions of mRNA of cdc2 and vitellogenin, with significantly increased E2 and VTG concentrations in hemolymph of female crayfish. However, a marked decrease of the E2 contents and spawning rate was observed in the groups treated with 30.27 and 59.76 μg Se/g diets. In conclusion, the results of this study indicated that the Se had maximum accumulation in ovary, affecting the reproductive capacity by intervening the expression of cdc2 and vitellogenin in the reproductive system. The LOAEL to induce FW was observed in crayfish fed with 10.02 μg Se/g diet, and its value can cause toxicity within the range of natural concentration, so the addition of Se in the feed should be within 10.02 μg Se/g.

Effect of selenium yeast supplementation on growth performance, feed utilization, lipid profile, liver and intestine histological changes, and economic benefit in meagre, Argyrosomus regius, fingerlings

This study was conducted to determine the effects of selenium (Se)-yeast supplementation on the growth, survival, nutrient utilization, plasma lipid profile, economic benefit, and histological alterations of liver and intestine of meagre, Argyrosomus regius, fingerlings (3.20 ± 0.17 g). Four treatments (three replicates each in 100 × 40 × 30 cm aquaria) included a control group (not supplemented with Se) and three groups fed Se-supplemented diets at doses of 1, 2 and 3 mg Se-yeast kg−1 diet for nine weeks. The final estimated Se concentrations were 0.77, 1.51, 2.97 and 3.98 mg Se kg−1 diet. The results indicated that there is a significant polynomial 2nd order regression improvement in growth performance with a strong correlation coefficient with increasing dietary Se concentrations. The estimated curves beak of WG and SGR values ranged between 4.15 and 3.62 mg Se kg−1, respectively. The survival (%), feed intake, feed and nutrient utilization and whole-body protein content increased significantly with the highest two Se doses (2.97 and 3.98 mg Se kg−1 diet). There was a significant increase in muscle Se accumulation with dietary Se supplementation in a linear dose-dependent manner. The total lipids, cholesterol and low-density lipoprotein cholesterol decreased significantly with increasing dietary Se levels. Moreover, the liver histological investigation showed an improvement in liver histostructure and the absence of pathological signs at high Se levels. Intestinal sections revealed an increase in the area of absorption in the Se-yeast-supplemented groups. The economic evaluation of feed cost kg−1 gain and kg−1protein production decreased with increasing Se-yeast supplementation levels, reaching a 41.63 and 39.57% cost reduction, respectively, with a 3 mg Se-yeast supplementation kg−1 diet. Dietary Se at a dose of 3.98 mg Se kg−1 diet (3 mg Se-yeast) is recommended to improve growth performance, feed utilization, liver and kidney histology, and the economic benefit of meagre juveniles. Additionally, the estimated Se requirement of meagre is around 4 mg kg−1 diet according to the polynomial 2nd order regression.

Effects of graded concentrations of supplemental selenium on selenium concentrations in tissues and prediction equations for estimating dietary selenium intake in pigs.

The experiment was conducted to determine the effects of graded dietary selenium (Se) on organ weight and Se concentrations in tissues and to develop equations for estimating dietary Se intake in pigs. Sixteen barrows (initial body weight = 30.0 ± 2.6) were allotted to four dietary treatments including graded Se supplementations with 0, 1, 5, and 50 mg/kg of diet. The experimental diets fed to the pigs for 30 d, and then the pigs were euthanized, and the organs, muscle, and urine samples were collected. The hair and blood samples of pigs were collected on d 15 and 30. Equations were developed for predicting daily Se intake using the Se concentration in plasma, hair, liver, kidneys, muscle, or urine. For graded dietary Se concentrations, linear and quadratic effects on the final body weight, weight and relative weight of liver and kidneys were not observed. The Se concentration in plasma, hair, liver, kidneys, muscle, and urine were linearly and quadratically increased as dietary Se concentration increased (P < 0.001). The dietary Se concentration was positively correlated with the Se concentrations in the plasma, organs, muscle, and urine (r > 0.81, P < 0.001). The equations for estimating dietary Se intake using the Se concentration in the plasma, hair, or organ as an independent variable were significant (P < 0.05). In conclusion, the dietary Se concentration was well reflected in the Se concentration in the plasma, hair, liver, kidneys, and urine. The Se concentration in the plasma, hair, liver, and kidneys can be used as an independent variable for estimating the Se intake.

Maternal Exposure to Dietary Selenium Causes Dopaminergic Hyperfunction and Cognitive Impairment in Zebrafish Offspring.

Maternal exposure to environmental contaminants is a predisposing factor for neurodevelopmental disorders with associated cognitive and social deficits in offspring. In this study, we investigated the effects of maternal exposure to selenium (Se), a contaminant of potential environmental concern in aquatic ecosystems, on cognitive performance and the underlying mechanisms in F1-generation adult zebrafish. Adult female zebrafish were exposed to different concentrations of dietary Se (3.5, 11.1, or 27.4 μg Se/g dry weight) for a period of 60 days. Fish were subsequently bred, and their offspring were collected and raised for 6 months on a normal diet. Maternal exposure to all concentrations of dietary Se induced learning impairment in F1-zebrafish tested in a latent learning task. The results also showed a hyperfunctioning dopaminergic system in fish exhibiting the learning deficit. The hyperfunction of the dopaminergic system was associated with enhanced oxidative stress and alterations in the mRNA abundance of several immediate early and late response genes in the zebrafish brain. Taken together, these results suggest that maternal exposure to dietary Se via alterations in the dopaminergic system leads to persistent neurobehavioral deficits in F1-generation adult zebrafish.

Dopaminergic dysregulation and impaired associative learning behavior in zebrafish during chronic dietary exposure to selenium

A growing body of evidence indicates that exposure to selenium (Se) can cause neurotoxicity, and this can occur because of its interference with several neurotransmitter systems in humans and animals. Dopamine is a critical modulator of a variety of brain functions and a prime target for environmental neurotoxicants. However, effects of environmentally relevant concentrations of Se on dopaminergic system and its neurobehavioral effects are still largely unknown. For this purpose, we exposed zebrafish, a model organism, to different concentrations of dietary l-selenomethionine (control, 3.5, 11.1, 27.4, and 63.4 μg Se/g dry weight) for a period of 60 days. Cognitive performance of fish was evaluated using a plus maze associative learning paradigm. Oxidative stress, as the main driver of Se neurotoxicity, was assessed by measuring the ratio of reduced to oxidized glutathione (GSH:GSSG), lipid peroxidation (LPO) levels, and mRNA expression of several antioxidant enzymes in the zebrafish brain. Dopamine levels in the brain and the expression of genes involved in dopamine synthesis, storage, reuptake, metabolism, and receptor activation were examined. Moreover, transcription of several synaptic plasticity-related immediate-early and late response genes was determined. Overall, fish fed with the two highest concentrations of dietary Se displayed impaired associative learning. Se exposure also induced oxidative stress in the zebrafish brain, as indicated by a reduction in GSH:GSSG ratio, increased LPO levels, and up-regulation of antioxidant genes in fish treated with the two highest concentrations of Se. An increase in brain dopamine levels associated with altered expression of dopaminergic cell markers was evident in different treatment groups. Moreover, Se exposure led to the down-regulation of immediate-early and late response genes in fish that exhibiting learning impairment. Taken together, the results of this study imply that the induction of oxidative stress and dysregulation of dopaminergic neurotransmission may underlie Se-induced impairment of associative learning in zebrafish.

Supplemented Organic and Inorganic Selenium Affects Milk Performance and Selenium Concentration in Milk and Tissues in the Guanzhong Dairy Goat.

Trace amounts of selenium (Se) are essential for several organisms, and deficiencies therein have adverse effects on growth, development, and reproduction; this is particularly significant in animals raised for milk and livestock production. To study the effect of Se on Guanzhong dairy goats, their diets were supplemented with different sources (inorganic or organic) and Se concentrations (0.2 or 0.4mgSe/kg). A non-Se-fortified basal diet served as a negative control, and a sixth treatment group received both inorganic and organic Se sources (0.2mgSe/kg diet each). Dietary Se supplementation increased milk production, with organic Se being more effective than inorganic Se. Selenium supplementation also increased Se concentration and glutathione peroxidase activity in whole blood, with organic Se more effective than inorganic Se at the same Se concentration. With increasing Se in diets, the Se content in milk increased markedly, reaching a plateau value at day 30 in all groups, and organic Se (0.4mg/kg diet) had the best effect. In addition, dietary Se sources and concentrations markedly affected Se concentrations in different tissues and organs. Thus, organic Se supplementation of a basal diet at 0.4mg/kg is practically applicable for Se-enriched milk and meat production in Guanzhong dairy goats.

Growth, antioxidant capacity and muscle histochemistry of yellowtail kingfish (Seriola lalandi Valenciennes 1883): Selenium and temperature interaction

The aim of this study was to investigate the interactive effects of temperature and dietary selenium concentrations on antioxidant capacity, muscle histochemistry and the growth, of juvenile yellowtail kingfish (Seriola lalandi). The yellowtail kingfish were exposed to two temperatures (21°C or 26°C) and three selenium levels (0.0, 2.0 or 4.0mgSekg−1 of feed) for 30 days. Final weight and specific growth rate (SGR) were significantly affected by water temperature (ρ<0.001) and dietary Se (ρ<0.001) supplementation, and there were significant differences in the interaction between these two factors. Juvenile yellowtail kingfish fed Se-supplemented diets, attained a higher final weight and SGR than those without Se supplementation at 21°C, but not at 26°C. Regardless of the temperature, the red blood cell (RBC) glutathione peroxidase (GPx) activity of yellowtail kingfish fed Se-supplemented diets was significantly higher (ρ<0.05) than with the control diet. However, GPx activity of yellowtail kingfish when fed either 2.0mgSekg−1 or 4.0mgSekg−1 showed no significant difference (ρ>0.05). Se concentration in the muscles of juvenile yellowtail kingfish fed Se-supplemented diets was higher than that of the yellowtail kingfish that were fed the control diet. A histopathological test confirmed that 20.3% of fish muscles exhibited lesions, which occurred in the absence of dietary Se. The outcome of the present study helps in understanding the interactive effects of dietary Se concentrations and the temperature in the farming of yellowtail kingfish.

Effect of Dietary Selenomethionine Supplementation on Growth Performance, Tissue Se Concentration, and Blood Glutathione Peroxidase Activity in Kid Boer Goats.

We used 240 kid Boer goats that were divided into six groups. The control group was fed a basal diet containing 0.05 mg of selenium (Se)/kg dry matter (DM). Trial groups received the basal diet supplemented with 0.1, 0.2, 0.3, 0.4, or 0.5 mg Se/kg DM (using a commercial selenomethionine product). Trial groups showed an improvement in growth performance (P < 0.05) despite no change in average daily feed intakes (ADFIs) (P > 0.05) compared to the control group A, quadratic model showed a correlation between glutathione peroxidase activity level in whole blood and dietary Se concentration (R(2) = 0.883, P < 0.04). The best linear model showed that increasing concentrations of Se in the blood (R(2) = 0.968, P < 0.001) and muscle (R(2) = 0.942, P < 0.001) corresponded to increasing Se concentrations in feed. Accumulation of Se in different tissues and organs corresponded to increasing Se concentrations in the diet as well as to the total time goats spent feeding on supplemented diet. Kidney and muscle tissues showed the highest and lowest accumulation of Se, respectively. Thus, Se in goat meat can be increased by adding between 0.1 and 0.5 mg/kg of selenomethionine to the diet of goats.

Characterization and Expression of Chicken Selenoprotein U.

Selenoprotein U (SelU) may regulate a myriad of biological processes through its redox function. In chicks, neither the nucleotide sequence nor the amino acid sequence is known. The main objectives of this study were to clone and characterize the chicken Selu gene and investigate Selu messenger RNA (mRNA) and protein expression in chicken tissues. The coding sequence (CDS) of Selu contained 387 bases with a typical mammalian selenocysteine insertion sequence (SECIS) located in the 3'-untranslated region. The deduced amino acid sequence of chicken SelU contains 224 amino acids with UAA as the stop codon. Like all SelU genes identified in different species, chicken SelU contains one well-conserved selenocysteine (Sec) at the 85th position encoded by the UGA codon. The SECIS element was with the conserved denosine (--AAA--) rather than the motif cytidine (--CC--) motif. Moreover, the expression pattern of Selu mRNA in muscle, liver, kidney, heart, spleen, lung, testis, and brain was analyzed with real-time quantitative PCR in young male chickens fed a Se-deficient corn-soybean meal basal diet supplemented with 0.0 and 0.3mg Se/kg in the form of sodium selenite. We found that the abundance of Selu mRNA in muscle, liver, kidney, heart, spleen, and lung was downregulated (P < 0.05) by Se deficiency. However, it was not affected by dietary Se concentrations in testis and brain. Furthermore, protein abundance of SelU in these seven tissues was consistent with the mRNA abundance. Hence, we suggest that Selu might play an important role in the biochemical function of Se in birds.