Dietary Protein Source Research Articles

Utilization of lactobacillus fermented proteins from meat processing wastewaters as a dietary protein source in poultry feed.

The present study aimed to evaluate the impact of fermented meat processing wastewater protein hydrolysate (FWMPH) prepared by Pediococcus lolii fermentation as an alternative protein source on growth performance, serum biochemistry, and hematological parameters of broiler chicks. FWMPH exhibited antioxidant, anti-angiotensin converting enzyme (ACE), and antimicrobial properties. A total of 60 one-day-old Vencobb broiler chicks were divided into five dietary groups on day 8 and a feeding trial was performed for 48days. Diets were prepared by replacing soya protein concentrate (SPC) at different levels of FWMPH as the protein source. These diets were denoted as SPSD (100% SPC), FMP-25 (25% FWMPH + 75% SPC), FMP-50 (50% FWMPH + 50% SPC), FMP-75 (75% FWMPH + 25% SPC), and FMP-100 (100% FWMPH). FMP-25 and FMP-100 diet group broiler chicks showed the highest and lowest feed consumption (P < 0.05), respectively. However, the highest body weight gain (1506.08 ± 52.52g) was observed in FMP-75 diet-fed chicks (P < 0.05). Furthermore, FMP-75 chicks displayed better feed conversion ratio (1.96), feed efficiency ratio (0.51), and protein efficiency ratio (2.32) values than other diet group chicks (P < 0.05), implying that the FMP-75 diet had better effect on the broiler growth. Also, the FMP-75 diet chicks showed better hematological values, serum biochemical parameters revealed no negative effect upon FWMPH consumption, and no pathological signs were observed in the histopathological examination as compared to the SPSD diet group (P < 0.05). Hence, the results could be helpful in the application of FWMPH at 75% level in the poultry feed as a potential protein ingredient in the countries looking for local feed protein products as soya bean meal replacement.

Contribution of Proteins to the Latin American Diet: Results of the ELANS Study.

Dietary protein intake is vital to life. Here we sought to characterize dietary sources of protein in eight Latin American countries. Survey data were collected for Estudio Latinoamericano de Nutrición y Salud (ELANS); participants were from Argentina, Brazil, Chile, Colombia, Costa Rica, Ecuador, Peru, and Venezuela (n = 9218, 15-65 years old). The primary aim of this analysis was to quantify per-person daily protein consumption by country and sociodemographic factors. Secondary aims: to quantify proportional intake of proteins by source, amount and processing, and to determine the adequacy of protein/essential amino acid intake. Younger groups (adolescents 15-19 years, adults 20-33 years) had the highest intake of proteins; middle-aged adults (34-49 years) had a lower intake, and older adults (50-65 years) had a strikingly lower intake. Protein consumption was higher in men than women. Animal proteins comprised nearly 70% of total daily protein intake in Argentina and Venezuela, contrasting with <60% in Peru, Chile, and Costa Rica. Brazil and Venezuela showed the highest protein intake within the highest education level. The higher the socioeconomic level, the higher the protein intake, except for Argentina, Chile, and Peru. Proportional intake of animal- and plant-based protein generally reflected the food availability by country. This study presents a pre-pandemic regional baseline and offers a perspective for future studies of changes related to government policies, climate, and dietary practices.

Plant and animal protein intake and its association with depression, anxiety, and stress among Iranian women

BackgroundMental disorders are conditions that affect the usual function of the brain, causing a huge burden on societies. The causes are often unclear, but previous research has pointed out, as is the case with many other diseases, that nutrition could have a major role in it. Amino acids, the building blocks of proteins, are the main precursor of neurotransmitters (the chemical messengers in the brain) malfunction of which is heavily associated with a wide range of brain disorders.MethodsWe assumed different sources of dietary protein could have different impacts on mental well-being. Hence, we decided to collect the nutritional data (with a validated and reliable semi-quantitative food-frequency questionnaire) from a sample of 489 Iranian women and investigate the association between animal and plant protein sources and the risk of depression, anxiety, and stress. Symptoms of these mental disorders were assessed using a validated Depression, Anxiety, and Stress Scales (DASS) questionnaire with 21 items.ResultsAfter multivariable adjustment, it was shown that women in the highest tertile of animal protein intake were more likely to show symptoms of depression (OR: 2.63; 95% CI: 1.45, 4.71; P = 0.001), anxiety (OR: 1.83; 95% CI: 1.04, 3.22; P = 0.03), and stress (OR: 3.66; 95% CI: 2.06, 6.50; p < 0.001). While no significant association was seen between plant protein and any of the studied mental disorders.ConclusionOverall, our findings suggest that a diet high in animal protein could predispose individuals to mental illnesses.

DUS Characterization and Evaluation of Untapped French Bean (Phaseolus vulgaris L.) Genotypes

French bean is being considered as an important legume for human diet having rich source of dietary proteins, minerals and vitamins. Germplasm characterization and evaluation is having great contribution in breeding and crop improvement. Substantial phenotypic and genetic characterization of untapped French bean germplasm are still needed to unlock its breeding potential. Purposefully, 27 diverse French bean genotypes comprising primitive, cultivars, commercial cultivars, farmers grown varieties were collected from different parts of India, characterized as per Distinct, Uniformity and Stability (DUS) and evaluated for associated morpho-phenological traits in randomized complete block design. The screened panel revealed highly significant differences among French bean genotypes for all the traits under study which witnessed the existence of sufficient variability in the French bean genotypes in which first two Principal Component Analysis (PCA) explained 20.03% and 13.32% of the total morphological variability. The genotypes were well scattered in PCA axis forming groups with various traits as per contribution towards the variability. The genotype IC 632961 having desirable traits which could be an elite germplasm for future crop improvement.

Effect of dietary Marula (Sclerocarya birrea caffra) nut meal on the growth performance, carcass traits and meat quality of Dorper lambs

Use of Marula nut meal (MNM) to replace Soyabean meal (SBM) as a dietary protein source in lamb fattening diets by determining effects on growth performance, carcass yield and meat quality was evaluated. MNM replaced SBM on a crude protein (CP) basis, in five diets, at 0%, 25%, 50%, 75% and 100%, respectively. Forty 112-day old entire male Dorper lambs (average body weight: 22.1 ± 3.3 kg) were randomly allocated to the diets and fed ad libitum for 63 days. Weekly and final body weight (FBW), body weight gain (BWG), average daily gain (ADG), daily feed intake (FI) and feed conversion ratio (FCR), warm and cold carcass weight, dressing percentage, meat (physical and chemical attributes) quality were determined. Dietary MNM caused no significant difference in FBW, BWG, ADG, FI, FCR, carcass weight and the meat’s physical attributes compared to SBM. At 25% and 100% dietary substitution of SBM (CP basis), MNM significantly (P < 0.05) reduced meat protein content. Feeding MNM-based diets produced meat with higher fat content compared to the control SBM diet (P < 0.05). The meat’s total monounsaturated fatty acid content, dominated by oleic acid, increased with increasing dietary MNM. At 100% substitution of SBM, dietary MNM produced meat with the highest (P < 0.05) oleic acid content. The results obtained in the study showed that MNM can replace SBM in lamb fattening diets without compromising growth performance, feed utilisation efficiency, carcass yield and physical attributes of meat. MNM can potentially be utilised to improve lamb quality by increasing its oleic acid content.

Evaluation of Cottonseed Meal as an Alternative to Fish Meal in Diet for Juvenile Asian Red-Tailed Catfish Hemibagrus wyckioides.

A 10-week trial was performed to investigate the effects of replacing fishmeal with cottonseed meal (CSM) on the growth rate, protein metabolism, and antioxidant response of Asian red-tailed catfish Hemibagrus wyckioides. Five isonitrogenous and isocaloric diets (C0, C8.5, C17.2, C25.7, and C34.4) were prepared to contain 0%, 8.5%, 17.2%, 25.7%, and 34.4% CSM replacing fishmeal, respectively. The weight gain, daily growth coefficient, pepsin, and intestinal amylase activities initially increased and then decreased with the raising dietary CSM levels; the highest values were observed in the C17.2 group (P < 0.05). However, feed cost exhibited the opposite trend. With the increasing dietary CSM levels, the protein efficiency ratio and intestinal trypsin activity decreased but feed conversion rate increased gradually; while no differences were observed among the C0, C8.5, and C17.2 groups (P > 0.05). Dietary CSM inclusion regardless of levels increased the plasma growth hormone level as well as hepatic aspartate aminotransferase (AST) and γ-glutamyl transpeptidase activities but decreased the plasma glutamate dehydrogenase and AST activities (P < 0.05). With the increasing dietary CSM levels, the plasma alkaline phosphatase (AKP) and hepatic superoxide dismutase activities decreased but malondialdehyde content increased gradually, while no differences were observed among the C0, C8.5, and C17.2 groups (P > 0.05). The plasma immunoglobulin M content and hepatic glutathione reductase activity initially increased but then decreased with the raising dietary CSM levels; the highest values were found in the C17.2 group. These results indicated that dietary CSM inclusion level up to 17.2% improved the growth rate, feed cost, digestive enzyme activity, and protein metabolism without compromising antioxidant capacity of H. wyckioide, whereas these parameters were depressed by further inclusion of CSM. CSM is a potentially cost-effective alternative plant protein source in diet of H. wyckioide.

Effects of dietary plant protein sources intake on growth, digestive enzyme activity, edible tissue nutritional status and intestinal health of the omnivorous Redclaw crayfish, Cherax quadricarinatus.

For the omnivorous Cherax quadricarinatus crayfish, plant raw materials can be good alternatives to dietary fish meal (FM). A 56-d feeding trial was conducted in C. quadricarinatus (11·70 (se 0·13) g). Diet with 100 % FM as the protein source was the control. Seven experimental diets were formulated by replacing 75 or 100 % of FM with soyabean meal (SM75, SM100) or cottonseed meal (CM75 and CM100), and a mixture of SM and CM (protein content is 1:1) replacing 50, 75 or 100 % of FM (SC50, SC75 and SC100). Crayfish fed the CM100 and SC100 showed significantly lower weight gain (WG), specific growth rate, trypsin and pepsin activities compared with the control diet. Crayfish in CM100 group showed significantly higher GPx, alanine aminotransferase, aspartate aminotransferase activities and malondialdehyde content than the control. SM100 and CM100 diets can cause slight separation of the peritrophic membrane from the intestinal folds. The pepsin activity of crayfish in SC50 was significantly higher than those in other experimental diets. The highest WG and muscle arginine content were also found in crayfish fed SC50. The relative abundance of Proteobacteria, Unclassified Enterobacteriaceae and Candidatus Bacilloplasma was significantly higher, but Actinobacteriota was significantly lower in SM100, CM100 and SC100 than in control. Microbiota functional prediction indicated that the relative abundance of 'cell motility' pathway in crayfish fed CM100 was significantly decreased compared with the control. In conclusion, only half of the FM can be effectively substituted with a mixture of SM and CM (protein content is 1:1) for C. quadricarinatus.

Evaluation of high-protein diets differing in protein source in healthy adult dogs.

Given the dynamic market for protein-based ingredients in the pet food industry, demand continues to increase for both plant- and animal-based options. Protein sources contain different amino acid (AA) profiles and vary in digestibility, affecting protein quality. The objective of this study was to evaluate the apparent total tract digestibility (ATTD) of canine diets differing in protein source and test their effects on serum metabolites and fecal characteristics, metabolites, and microbiota of healthy adult dogs consuming them. Four extruded diets were formulated to be isonitrogenous and meet the nutrient needs for adult dogs at maintenance, with the primary difference being protein source: 1) fresh deboned, dried, and spray-dried chicken (DC), 2) chicken by-product meal (CBPM), 3) wheat gluten meal (WGM), and 4) corn gluten meal (CGM). Twelve adult spayed female beagles (body weight [BW] = 9.9 ± 1.0 kg; age = 6.3 ± 1.1 yr) were used in a replicated 4 × 4 Latin square design (n = 12/treatment). Each period consisted of a 22-d adaptation phase, 5 d for fecal collection, and 1 d for blood collection. Fecal microbiota data were analyzed using QIIME 2.2020.8. All other data were analyzed using the Mixed Models procedure of SAS version 9.4. Fecal scores were higher (P < 0.05; looser stools) in dogs fed DC or CBPM than those fed WGM or CGM, but all remained within an appropriate range. Dry matter ATTD was lower (P < 0.05) in dogs fed CBPM or CGM than those fed DC or WGM. Crude protein ATTD was lower (P < 0.05) in dogs fed DC or CGM than those fed WGM. Dogs fed CBPM had lower (P < 0.05) organic matter, crude protein, and energy ATTD than those fed the other diets. Fecal indole was higher (P < 0.05) in dogs fed CBPM than those fed WGM. Fecal short-chain fatty acids were higher (P < 0.05) in dogs fed DC than those fed CGM. Fecal branched-chain fatty acids were higher (P < 0.05) in dogs fed DC or CBPM than those fed WGM. Fecal ammonia was higher (P < 0.05) in dogs fed DC or CBPM than those fed WGM or CGM. The relative abundances of three bacterial phyla and nine bacterial genera were shifted among treatment groups (P < 0.05). Considering AA profiles and digestibility data, the DC diet protein sources provided the highest quality protein without additional AA supplementation, but the animal-based protein diets resulted in higher fecal proteolytic metabolites. Further studies evaluating moderate dietary protein concentrations are needed to better compare plant- and animal-based protein sources.

Primary source of dietary protein is correlated with differences in the intestinal microbiome diversity

In spite of the recent popularization of the vegan diet, there is currently a deficit of studies that compare the impact and associated health benefits of dietary protein source, one of the main differentiators between vegan and non-vegan diets, on the intestinal microbiome. The objective of this study was therefore to correlate observed changes in the intestinal microbiome to changes in dietary protein sources. We hypothesized that changes in dietary protein source are associated with changes in the composition of the intestinal microbiome. The participant changed their diet over four separate one-week periods to include plant proteins only, plant proteins and dairy proteins, animal proteins only, and finally animal proteins and dairy proteins. The participant followed their respective dietary treatments for one week. Bacterial 16S dual-index sequencing and bioinformatic analysis identified seven genera and two families of bacteria. Of these, Bifidobacterium, Bacteroides, Lactobacillum, and Clostridium exhibited population trends correlated with changes in diet. Moreover, a comparison of α-diversity indicated a statistically significant change over the course of the experiment. Although the results of this experiment cannot be generalized due to the sample size, the hypothesis of this experiment was validated: changes in dietary protein source are correlated with changes in the diversity of the intestinal microbiome. However, we recommend further research into the impacts of variance in dietary protein source on the microbiome, especially by implementing longer-term dietary treatments.

Effect of Incorporating Cowpea (&amp;lt;i&amp;gt;Vigna unguiculata&amp;lt;/i&amp;gt;) Meal into Broiler Diets on Environmental Pollution by Nitrogen from Poultry Excreta: A Review

Poultry feeds are formulated using soybean meal and animal by-products as sources of protein. Animal proteins like fish meal, blood meal, meat and bone meal are being shunned in formulating poultry feeds nowadays because they are associated with zoonotic diseases like salmonellosis and this has left soybean meal as the only source of dietary protein in broiler feeds. Soybean meal is in short supply in Zimbabwe and this country is relying on some soybean meal imports from South Africa and Zambia resulting in prices of poultry feed and broiler meat going upwards. Cheap and locally available alternative sources of protein to soybean meal must be found in order to reduce the cost of making poultry feed. The selected alternative source of protein must have protein which is highly digestible such that the bulk of this protein can be metabolized and utilized by broilers to synthesize meat leaving a little of it to be excreted through faeces and urine. Highly digestible protein is very important in broiler feed making because this reduces the amount of nitrogen lost through poultry excreta into the environment. Cowpea has been chosen to entirely or partially replace soybean meal in this review paper because it is locally grown, drought tolerant, cheap and its true protein digestibility (TPD) of 71% to 76% is generally comparable to 81% to 83% of soybean meal. Nowadays, people are concerned about protecting the environment from being polluted by wastes from industrial and agricultural activities. Poultry farming pollutes the environment with ammonia emitted from poultry excreta. The grain legumes used in formulating broiler feed such as soybean meal contain anti-nutritional factors which reduce protein digestibility and increase nitrogen excretion through poultry faeces. The nitrogen in faeces is volatilized into ammonia, emitted into the atmosphere and cause eutrophication of surface waters. Therefore, the effect of incorporating cowpea meal in broiler diets on environmental pollution by nitrogen excretion from broiler faeces needs to be explored.

Cross-Sectional Study for Investigation of the Association Between Modifiable Risk Factors and Gastrointestinal Cancers at a Tertiary Hospital in Ghana.

Malignancies affecting the gastrointestinal tract are among the principal threats to global public health. In Ghana, these cancers are responsible for a significant number of hospitalizations and mortalities at major health facilities across the country. The increasing incidence of these malignancies necessitates an investigation of the association between lifestyle (modifiable risk factors) and these disorders. To determine the association between lifestyle and gastrointestinal cancers of patients attending the Korle Bu Teaching Hospital (KBTH). This was a cross-sectional prospective study where demographic data were obtained from consenting patients diagnosed with gastrointestinal cancer at the oncology and surgical clinics of the KBTH. Diagnostic investigations, gastrointestinal cancer phenotype, year of diagnosis and treatment(s) received were also obtained from the participants. Information on smoking status, alcohol consumption, sources of dietary proteins, daily intake of water, and frequency of fruit intake were also obtained from the participants. Odds ratio and P-values were determined to ascertain whether there might be a significant association between gastrointestinal cancers and specified lifestyle. Colorectal cancers were the most prevalent form of gastrointestinal cancers among the participants. Alcohol consumption or smoking habits were not significantly associated with onset of gastrointestinal cancers among the study participants. There was a significant association but weak correlation between red meat consumption and the colorectal cancer. This study shows consumption of red meat to be a modifiable risk factor that is associated with lower gastrointestinal cancers in the study participants. Further longitudinal studies using large number of participants is needed for confirming the observations from this current study.

PS-BPP06-6: THE ASSOCIATION BETWEEN CARBON AND NITROGEN STABLE ISOTOPE RATIOS OF HUMAN HAIR AND HYPERTENSION

Background: The relationship between stable isotope ratios and dietary protein sources has been reported. However, few studies have examined the effect of stable isotope ratios on metabolic risk in humans. We investigated whether the stable isotope ratios of carbon and nitrogen in human hair are associated with blood pressure and hypertension. Methods: We conducted a cross-sectional study of 392 subjects (228 men and 164 women). Hair samples of the subjects were used for the measurement of stable isotope ratios of carbon (Δ13C) and nitrogen (Δ15N). Results: The Δ13C and Δ15N values showed positive correlations with diastolic blood pressure in the subjects without antihypertensive medication. In the subjects without antihypertensive medication, the multivariable-adjusted odds ratio (95% confidence interval) for hypertension was 1.55 (1.04 - 2.30) per 1‰ increase in Δ15N and 1.22 (0.86 - 1.73) per 1‰ increase in Δ13C, respectively. However, in the subjects with antihypertensive medication, neither Δ13C nor Δ15N values showed a significant association with hypertension. Conclusions: The stable isotopic ratio of nitrogen in scalp hair is independently associated with hypertension in subjects without antihypertensive medication. The hair Δ15N value might be used as a surrogate marker to screen a high-risk population for hypertension.

Yeast protein as a novel dietary protein source: Comparison with four common plant proteins in physicochemical properties

Currently, with the preference for a healthy diet and increased awareness of reducing the carbon footprint, the demand for protein is becoming more and more diversified. In this study, the physicochemical properties of yeast protein (YP) and four common plant proteins (soy protein isolate, pea protein isolate, wheat gluten, and peanut protein) were compared. The most prevalent secondary structure in YP is the β-sheet. Furthermore, YP is in an aggregated state, and it has a high surface hydrophobicity. The tryptophan residues are primarily exposed on the polar surface of YP. The results of in vitro digestibility indicated that YP (84.91 ± 0.52%) was a high-quality protein. Moreover, YP has a higher thermal stability and relatively stable low apparent viscosity, which provides ample possibility for its application in food processing and in foods for people with swallowing difficulties. This study provides theoretical basis in the potential of YP as an alternative protein source.

In vivo study of the biological value of amaranth protein concentrate and its module with chicken egg protein

Amaranth (Amaranthus L.), like other pseudocereals as quinoa (Chenopodium quinoa Willd.), chia (Salvia hispanica L.) and buckwheat (Fagopyrum sp.), is a promising source of dietary protein. Depending on the subspecies and breeds of amaranth, the protein content in its grain is estimated from 13.1 to 21.5%, and its amino acid score varies over a significant range and can be limited. The aim of this study was to obtain a protein concentrate from amaranth (Amaranthus L.) grain of the Voronezh breed, enrich it with chicken egg protein, determine the amino acid score of the obtained protein module, and experimentally evaluate in vivo its true digestibility and biological value. Material and methods. The amaranth protein concentrate was obtained from grain according to the technological scheme, including its enzymatic treatment, alkaline extraction, acid precipitation of proteins, microfiltration and lyophilization. The amino acid composition and amino acid score of the concentrate were determined. The protein module was obtained by mixing amaranth protein concentrate and chicken egg protein in a weight ratio of 58:42. The true digestibility and biological value of the protein module has been determined in vivo. The experiment was carried out on 32 Wistar male rats divided into 2 groups (n=16 rats): control group 1 with a body weight of 118.7±3.1 g and experimental group 2 with a body weight of 119.5±3.0 g. Animals of groups 1 and 2 received diets in which egg protein and a protein module were used as a protein source, respectively. Within 15 days of the experiment, individual indicators of food intake and body weight gain of each animal were determined. From the 14th to the 15th day food intake was determined and feces were collected. The amount of nitrogen in the food and feces was determined for each rat using the Kjeldahl method. The true digestibility of the protein was determined according to obtained data. Results. The resulting amaranth protein concentrate contained 70.4±0.6% of protein, 17.0±1.0% fat, 9.8±0.8% carbohydrates, 1.8±0.2% ash, its moisture content was 1.4±0.1%. There were no significant differences in food intake and body weight gain between animals of both groups. The calculated value of the true digestibility of chicken egg protein was 98.8±0.1% for the control group 1, of the protein module was 99.0±0.1% for the experimental group 2, the differences between the groups were not significant. Conclusion. The results of amino acid analysis and the in vivo study of the true digestibility of the protein module (composition amaranth protein/chicken egg protein) indicate the absence of limitation relative to the amino acid scale of the "ideal" protein (FAO/WHO, 2007) and high true digestibility. The biological value of the protein module, calculated according to PDCAAS, is 99.0±0.1%, which confirms the prospects for its inclusion in specialized foods.

Effect of high-intensity ultrasound on the physicochemical properties of Tenebrio Molitor Protein

Insect is an important source of dietary protein, and its application in the food industry has attracted attention. Tenebrio Molitor is rich in water-soluble protein, and the over-hydrophilic nature of water-soluble protein generally limits its industrial application. For the above reasons, this study systematically investigated the effect of high-intensity ultrasound (HIUS) on the physicochemical and physical characteristics of Tenebrio Molitor Protein (TMP). The results show that HIUS treatment might break the non-covalent bonds through the cavitation effect generated by the ultrasound probe, inducing the unfolding and disintegration of TMP soluble aggregates, and resulting in hydrophobic exposure and particle size reduction. HIUS optimize the physical characteristics of TMP (solubility, foamability, foam stability, emulsification, etc.) by changing its secondary/tertiary structure. Furthermore, emulsions stabilized by HIUS-treated TMP (UTMPEs) present smaller particle size and denser structure due to the changes in secondary structure, which plays a dominant role in the protein interface adsorption stabilization. The results of this study are expected to provide new guidance for better application of HIUS-treated TMP in the future food industry. • Modification of Tenebrio Molitor Protein (TMP) using high-intensity ultrasound (HIUS). • HIUS changes the secondary and tertiary structure of TMP. • HIUS enhances the emulsifying ability of TMP.

Evaluation of modified dried vinasse as an alternative dietary protein source for broilers

AbstractThe increase in poultry production and the high cost of soybean led to the search for alternative protein sources. One of these sources is vinasse, a by‐product of the baker's yeast industry. Modified dried vinasse (MDV) can be produced for use in poultry nutrition by making some improvements in vinasse. Therefore, the present study aimed to examine the effect of the usage of MDV in broiler diets. A total of 192 daily male Ross 308 chicks were randomly assigned to four groups. MDV was included at the levels of 0%, 2%, 4%, and 6% in the diets for 42‐day trial. Linear significant improvements in the final weight, body weight gain, feed efficiency, and digestibility were seen with increasing MDV levels. The use of MDV caused a significant reduction in feed consumption. The relative weight percentages of abdominal fat and serum cholesterol concentration were reduced linearly with increases in MDV levels. MDV inclusion linearly decreased the malondialdehyde concentration, but increased 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging activity in breast meat significantly. The protein content in breast meat was increased with MDV. Cecal beneficial microorganisms and serum IgG levels were increased linearly with MDV. In conclusion, results suggested that MDV could be a feasible option for alternative protein sources for broilers.

Effect of soaking and extrusion on functional and pasting properties of cowpeas flour

Cowpea (Vigna anguiculata) is a leguminous crop that is widely grown and consumed in Asia and Africa as a major source of dietary protein. This study investigated the effect of varying extrusion conditions (temperature and moisture content) on the functional and pasting properties of cowpea flour. Particle size distribution, functional and pasting properties of cowpeas flour were determined. Moisture content had a significant effect on the functional and pasting properties of cowpeas flour followed by temperature. Increasing moisture content from 10% to 15%, increased the bulk density from 0.4 to 0.5, Oil Absorption Capacity 149 to 238, Water Absorption Capacity 407 to 422, Swelling Power 4.6 to 4.9, Water Absorption Index 3.9 to 4.2, as well as Peak & Breakdown viscosities. On the other hand, reducing the moisture content decreased Water Solubility Index (WSI) from 31 to 21. Lower extrusion temperature increased all the functional properties except WSI along with pasting properties namely Peak, Final and setback viscosities. Higher extrusion temperature increased WSI, breakdown, peak time, and pasting temperature. The results suggest that variation of extrusion conditions can be used to modify the functional and pasting properties of cowpea flour to suit different applications.

Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats

BackgroundPlant proteins (PPs) have been associated with better cardiovascular health than animal proteins (APs) in epidemiological studies. However, the underlying metabolic mechanisms remain mostly unknown. ObjectivesUsing a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context. MethodsMale Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively. We measured body weight and composition, hepatic enzyme activities and lipid content, and plasma metabolites. In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method. Data were analyzed using ANOVA and Mixed models. ResultsAt the whole-body level, PP limited HFS-induced insulin resistance (–27% in HOMA-IR between HFS groups, P < 0.05). In the liver, PP induced lower lipid content (–17%, P < 0.01) and de novo lipogenesis (–24%, P < 0.05). In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass. ConclusionsIn growing rats, compared with AP, a balanced blend of PP similarly supports protein anabolism while better limiting whole-body and tissue metabolic dysregulations through mechanisms related to their less optimal AA profile for direct channeling to PS.

Comparison of the Nutrient Compositions in Red and Green Amaranthus (Amaranthus hypochondriacus)

Amaranth has two morphological types, one is red and another is green. For this study, we collected five red and five green morph samples to analysis in terms of proximate, minerals, antioxidant pigments and phytochemicals and antioxidant activity in three replicates. The leaves of red amaranth are an outstanding source of dietary fiber, carbohydrates, moisture, and protein. We found remarkable potassium (6.55 mg/g), calcium (2.63 mg/g), magnesium (3.01 mg/g), iron (10.94 µg/g), manganese (13.16 µg/g), copper (2.01 µg/g), zinc (11.57 µg/g), carotenoids (47.13 mg/100g), total phenolics (14.36 GAEµg/g), vitamin C (50.74 mg/100g) and antioxidant activity (ABTS+) (25.27 TEACµg/g) in the red amaranth leaves. These data indicated that red and green could be considered as enriched in their antioxidant profiles. Carotenoids exhibited significant positive associations with TPC, TFC and TAC (ABTS+). Vitamin C had a minor and positive interrelationship with TAC, TFC, and TPC, although it exhibited negative and insignificant associations with carotenoids. TPC, TFC and TAC (ABTS+) showed a significant positive association. Red amaranth is an excellent source of nutrients, antioxidant pigments, minerals, and phytochemicals compared to green amaranth. In this investigation, it was revealed that flavonoids, phenolic compounds and carotenoids had strong antioxidant activity and significantly contributed to the antioxidant activity of the green and red of amaranth. Red amaranth could be a potential source of nutritional components and antioxidant phytochemicals in the human diet providing opportunities to address mineral nutrient deficiencies and provide an antioxidant rich food.

Analysis of Cr (VI) accumulation from feed to broiler chicken by using spectroscopy

Broiler chicken is one of the major dietary protein sources in Bangladesh. This study is aimed to investigate the status of chromium (VI) content in broiler feeds and water, as well as the risk of chromium (VI) in broiler meat, liver, and brain. For this investigation, a total of five broiler-rearing farms were chosen, each with a different type of broiler feed. A total of fifteen broiler chickens along with the water and feed samples were collected from those broiler farms. The concentration of chromium (VI) in feed, water, broiler meat, liver, and brain samples was determined using an Atomic Absorption Spectrophotometer (AAS). Two feed samples (Farm B and Farm D) were found to be chromium (VI) polluted (3.157±0.050 mg/Kg and 3.510±0.095 mg/Kg respectively), while water samples were found to be devoid of contamination. Only meat, liver, and brain samples from several farms were found to have chromium (VI). Brain samples exhibited the highest concentration of chromium (3.550±1.281 mg/Kg) compared to the liver (2.700±1.084 mg/Kg) and muscle meat (0.857±0.076 mg/Kg) samples. The amounts of chromium (VI) in the broiler brain and liver were found to be substantially higher than FAO and WHO acceptable standards. The rate of chromium (VI) concentration in meat samples was below the allowed level. It is proposed that precautions should be taken to detect chromium in chicken feed to assess health hazards and protect humans from food that could have serious negative consequences for their health. Asian J. Med. Biol. Res. 2022, 8 (4), 225-229