Dietary Lipid Research Articles

Effects of dietary lipid levels on lipid accumulation and health status of adult Onychostomamacrolepis

The effects of different dietary lipid levels on lipid accumulation, inflammatory response, serum bio-chemical index and histological features of intestine and hepatopancreas of O.macrolepis was experimentally evaluated in an eight-weeks study. Fish (initial weight 50.11 ± 2.86 g) were fed with five iso-nitrogenous diets (around 390 g/kg protein) varying with lipid level (5%, 7%, 9%, 11%, 13%, being LL5, LL7, LL9, LL11, and LL13 respectively) in triplicates. Results showed that the content of crude lipid in carcass and hepatopancreas were not affected by dietary lipid levels (P > 0.05). Serum ALT, TP, HDL-c and MDA, etc., were not significantly affected by diets (P > 0.05), while serum total antioxidant capacity in LL9 and LL11 groups were significantly higher than the other groups (P < 0.05). Histological features of hepatopancreas and intestine showed no significant difference among the five diets (P > 0.05), while the height of intestine villus showed the higher trend in LL9 compared with other groups. The relative expression of lipid metabolism related genes (ppar α, cpt-1α, fas, and hsl) and immune response related genes (tlr 22, nrf 2, tnfα, and il-γ) in the hepatopancreas of fish fed diets differing in their crude lipid levels were not significantly different (P > 0.05). The results suggested that a proper dietary lipid level of 9%–11% could maintain higher antioxidant and health status of adult O.macrolepis.

Peroxisome Proliferator-Activated Receptor Signaling-Mediated 13-S-Hydroxyoctadecenoic Acid Is Involved in Lipid Metabolic Disorder and Oxidative Stress in the Liver of Freshwater Drum, Aplodinotus grunniens.

The appropriate level of dietary lipids is essential for the nutrient requirements, rapid growth, and health maintenance of aquatic animals, while excessive dietary lipid intake will lead to lipid deposition and affect fish health. However, the symptoms of excessive lipid deposition in the liver of freshwater drums (Aplodinotus grunniens) remain unclear. In this study, a 4-month rearing experiment feeding with high-fat diets and a 6-week starvation stress experiment were conducted to evaluate the physiological alteration and underlying mechanism associated with lipid deposition in the liver of A. grunniens. From the results, high-fat-diet-induced lipid deposition was associated with increased condition factor (CF), viscerosomatic index (VSI), and hepatosomatic index (HSI). Meanwhile, lipid deposition led to physiological and metabolic disorders, inhibited antioxidant capacity, and exacerbated the burden of lipid metabolism. Lipid deposition promoted fatty acid synthesis but suppressed catabolism. Specifically, the transcriptome and metabolome showed significant enrichment of lipid metabolism and antioxidant pathways. In addition, the interaction analysis suggested that peroxisome proliferator-activated receptor (PPAR)-mediated 13-S-hydroxyoctadecenoic acid (13 (s)-HODE) could serve as the key target in regulating lipid metabolism and oxidative stress during lipid deposition in A. grunniens. Inversely, with a lipid intake restriction experiment, PPARs were confirmed to regulate lipid expenditure and physiological homeostasis in A. grunniens. These results uncover the molecular basis of and provide specific molecular targets for fatty liver control and prevention, which are of great importance for the sustainable development of A. grunniens.

Physico-Chemical and Nutritional Properties of Chia Seeds from Latin American Countries.

In the last few decades, chia (Salvia hispanica L.) cultivation has expanded around the world, and the seeds have become well known due to their rich composition of nutrients and bioactive compounds. The aim of this work was to evaluate the physical, chemical, and nutritional profile of eight types of chia seeds grown in different Latin-American countries (Argentina, Bolivia, Chile, Ecuador, Mexico, Paraguay, and Peru). The results showed that several nutritional parameters of the seeds, such as the protein content and amino acid profile, dietary fiber content, lipid content, mineral composition, and presence of phytate, depend on the location in which they were grown. Other parameters, such as ash content, fatty acid profile, or various physical parameters, were uniform across locations (except for color parameters). The results support the notion that the nutritional characteristics of seeds are determined by the seeds' origin, and further analysis is needed to determine the exact mechanisms that control the changes in the seed nutritional properties of chia seeds.

Effects of Dietary Protein and Lipid Levels in Practical Formulation on Growth, Feed Utilization, Body Composition, and Serum Biochemical Parameters of Growing Rockfish Sebastes schlegeli.

A 3 × 2 factorial experiment (protein levels, 42%, 46%, 50%; lipid levels, 9%, 12%) with three replicates was conducted in a circulating water system to investigate the effects of dietary protein and lipid levels on growth, feed utilization, body composition, and serum biochemical parameters of growing rockfish Sebastes schlegeli (initial weight, 29.98 ± 0.10 g). After an 8 weeks feeding trial, growth performance in terms of final body weight, percent weight gain, and specific growth rate increased with the increase of dietary protein level when fish fed diets containing a consistent level of dietary lipid. The feed conversion rate and daily feed intake were significantly affected by dietary protein and lipid levels, and decreased as dietary protein level increased from 42% to 46% or dietary lipid level increased from 9% to 12% (P < 0.05). Survival rate, viscerosomatic index, and hepatosomatic index were unaffected by dietary protein level (P > 0.05), but significantly increased with the increase of dietary lipid level (P < 0.05). On the contrary, condition factor was unaffected by dietary lipid level (P > 0.05), but significantly increased with dietary protein level increasing up to 46% (P < 0.05). The moisture contents of muscle and liver significantly decreased, but the whole-body crude lipid content, the crude protein and lipid contents of muscle increased as dietary protein or lipid level increased (P < 0.05). The contents of isoleucine, leucine, histidine, glycine, alanine of muscle, as well as the proportions of C14 : 0, C20 : 1, and C22 : 1n-9 in total fatty acids were higher in fish fed diets containing 12% lipid than those fed 9% lipid (P < 0.05), while C18 : 1n-9 and C18 : 2n-6 followed an opposite trend. The contents of phenylalanine, lysine, and tyrosine as well as the proportions of C18 : 0, C18 : 2n-6, C22 : 1n-9, and C22 : 6n-3 in total fatty acids decreased with the increase of dietary protein level (P < 0.05). Serum cholesterol and low-density lipoproteins increased significantly with dietary protein or lipid levels increasing, but TG concentration was elevated significantly in fish fed diets containing 12% lipid. Considering the present results in terms of growth and feed utilization, the suitable protein and lipid levels in diet for growing rockfish were 46% and 12%, respectively.

Plasma Fatty Acid Composition, Oxidative and Inflammatory Status, and Adherence to the Mediterranean Diet of Patients with Non-Alcoholic Fatty Liver Disease.

Non-alcoholic fatty liver disease (NAFLD) is a complex and increasingly prevalent cardiometabolic disorder worldwide. As of today, NAFLD is a pathology without specific pharmacological treatment, with the Mediterranean diet (MedDiet) being the most widely used approach for its management. The objective of this study is to assess the effects of adherence to the Mediterranean diet on fatty acid plasma levels, as well as on the oxidative and inflammatory status of NAFLD patients. A total of 100 adult patients (40-60 years old) diagnosed with NAFLD and from the Balearic Islands, Spain, were classified into three groups according to their adherence to the MedDiet. Consumption was assessed using a validated 143-item semiquantitative Food Frequency Questionnaire. Food items (g/day) were categorised according to their processing using the NOVA system. Anthropometrics, blood pressure, aminotransferases, Dietary Inflammatory Index (DII), inflammatory biomarkers, and fatty acid levels were measured in the plasma of NAFLD patients. High adherence to the MedDiet is associated to a highly plant-based diet, low ultra-processed food (UPF) consumption, low intake of dietary lipids, low intake of animal fats, high intake of monounsaturated fatty acid (MUFA; mainly palmitoleic acid), low intake of saturated fatty acids (SFAs; practically all dietary SFAs), low intake of trans-fatty acids, high intake of omega-3 fatty acids (mainly eicosapentaenoic acid), a higher n-6:n-3 in ratio, low intake of omega-6 fatty acids, and a low level of interleukin-6 (IL-6). High adherence to the MedDiet is related to a better fatty acid profile in the plasma, fewer SFAs and more MUFA and polyunsaturated fatty acids (PUFAs), a plasma biochemical profile, better proinflammatory status, and decreased ultra-processed food consumption of NAFLD patients.

Reassessment of dietary protein and lipid requirements for large yellow croaker, Larimichthys crocea, reared in net pens

AbstractAt present, large yellow croaker, Larimichthys crocea, a carnivorous fish ranked fifth in aquaculture production among marine finfishes in the world, still relies on raw fish diet because formulated feed alone is not able to sustain rapid growth of the fish. The present study evaluated growth, feed utilization efficiency, body composition and antioxidant capacity of large yellow croaker fed diets containing four protein (430, 460, 490 and 520 g/kg) and three lipid (60, 90 and 120 g/kg) levels. Fish weighing 11.4 ± 2.8 g were fed with the test diets in net pens for 8 weeks. Final body weight and nitrogen retention efficiency (NRE) were dependent on dietary protein and lipid levels and their interaction. Weight gain, body protein content, serum catalase (CAT) activity and liver glutathione peroxidase (GSH‐Px) activity were dependent on dietary protein and lipid levels. Phosphorus retention efficiency was dependent on dietary protein level and interaction between protein and lipid levels. Hepatosomatic index, body lipid and energy contents, activities of superoxide dismutase (SOD) and GSH‐Px in serum, and CAT activity and malondialdehyde content in liver were dependent on dietary lipid level. The final body weight and weight gain of fish fed at 490 g/kg protein level did not significantly differ from those fed at 520 g/kg protein level. The final body weight, weight gain and NRE of fish fed at 90 g/kg lipid level were higher than those fed at 60 g/kg lipid level, but did not significantly differ from those fed at 120 g/kg lipid level. The maximum weight gain occurred in fish fed the diets either containing 520 g/kg protein or 90 g/kg lipid. The body lipid content, as well as SOD and GSH‐Px activities in serum, were higher in fish fed the diets with 90 and 120 g/kg lipid compared to fish fed the diets with 60 g/kg lipid. The present study reveals that the optimal dietary protein and lipid levels for large yellow croaker reared in net pens were 490–520 g/kg and 90 g/kg, respectively.

Oligofructose improves small intestinal lipid-sensing mechanisms via alterations to the small intestinal microbiota

BackgroundUpper small intestinal dietary lipids activate a gut-brain axis regulating energy homeostasis. The prebiotic, oligofructose (OFS) improves body weight and adiposity during metabolic dysregulation but the exact mechanisms remain unknown. This study examines whether alterations to the small intestinal microbiota following OFS treatment improve small intestinal lipid-sensing to regulate food intake in high fat (HF)-fed rats.ResultsIn rats fed a HF diet for 4 weeks, OFS supplementation decreased food intake and meal size within 2 days, and reduced body weight and adiposity after 6 weeks. Acute (3 day) OFS treatment restored small intestinal lipid-induced satiation during HF-feeding, and was associated with increased small intestinal CD36 expression, portal GLP-1 levels and hindbrain neuronal activation following a small intestinal lipid infusion. Transplant of the small intestinal microbiota from acute OFS treated donors into HF-fed rats also restored lipid-sensing mechanisms to lower food intake. 16S rRNA gene sequencing revealed that both long and short-term OFS altered the small intestinal microbiota, increasing Bifidobacterium relative abundance. Small intestinal administration of Bifidobacterium pseudolongum to HF-fed rats improved small intestinal lipid-sensing to decrease food intake.ConclusionOFS supplementation rapidly modulates the small intestinal gut microbiota, which mediates improvements in small intestinal lipid sensing mechanisms that control food intake to improve energy homeostasis.7qHK-ay1HXaqfvyHrZSPZbVideo

HPLC-CAD method to quantify lipolysis products from plant-based oils rich in unsaturated fatty acids

Dietary lipids such as oils and fats are mainly composed of triglycerides. These macronutrients play diverse roles in food products from sensorial, over structural to nutritional. Hence, as part of nutrition and health, it is essential to understand biochemical processes occurring during lipid digestion. To this end, it is indispensable to rely on consistent analytical techniques that enable detection, identification, and quantification of lipolysis products. This work proposes a reversed-phase high performance liquid chromatography with a charged aerosol detector (RP-HPLC-CAD) method that permits quantification of the most abundant lipid species present in highly consumed plant-based oils rich in unsaturated fatty acids and the lipid digestion products derived thereof (e.g. oleic acid, linoleic acid, 1-monolinolein, 1/3-monoolein, 1-linolein, 2-olein, 1/3-diolein, triolein, and 1,2-diolein-3-linolein). The development included gradient program and detector settings optimisation along with validation. Additionally, the method was evaluated in terms of lipid digestion kinetics of oil-in-water emulsions. CAD exhibited high sensitivity and allowed the detection of analytes at nano levels (from 1.5 to 9.3 ng). Moreover, the resulting method demonstrated to be simple, quick, robust and able to detect all the analytes with high sensitivity and precision. Importantly, it allowed a better understanding of molecular changes occurring during lipid hydrolysis.

Metabolomic and transcriptomic profiling reveals the effect of dietary protein and lipid levels on growth performance in loach (Paramisgurnus dabryanus).

The subject of this study was to explore the optimum requirements of loach (Paramisgurnus dabryanus) regarding dietary proteins and lipids and discuss the underlying mechanism. We designed nine diets to determine the effects of different levels of dietary crude protein (CP: 30%, 35%, and 40%) and ether extract (EE: 6%, 10%, and 14%) on the growth performance and metabolism of P. dabryanus. In total, 2160 healthy P. dabryanus (5.19 ± 0.01g) were divided into nine groups with four replications at 60 fish per barrel stocking density. The trial lasted for eight weeks. Serum and liver samples were gathered for metabolomic and transcriptomic analyses. The results showed that the specific growth rate of P. dabryanus in the CP40EE10 group was the fastest and notably higher than that in other groups (P< 0.05). Analysis of the metabolome results found that the mTOR signaling pathway, glycerophospholipid metabolism, D-arginine and D-ornithine metabolism were significantly enriched pathways in the CP40EE10 group compared with the other groups (P< 0.05). Moreover, the transcriptomic analysis of differentially expressed genes (DEGs) showed that the expression of ARG (arginase) involved in protein synthesis was significantly upregulated in the CP40EE10 group compared to the slowest growing group (P< 0.05). Additionally, the expression of SPLA2 (secretory phospholipase A2) involved in lipid metabolism and FBP (fructose-1,6-bisphosphatase) involved in glucose metabolism were all significantly downregulated in the CP30EE6 group compared with the CP40EE10 group (P< 0.05). Furthermore, the analysis of differentially expressed metabolites (DEMs) and DEGs co-enriched in the KEGG pathway revealed that the significantly enriched pathways were arginine and proline metabolism, glycerophospholipid metabolism, and glycolysis/gluconeogenesis in CP40EE10 compared with other groups (P< 0.05). We conclude that including 40% CP and 10% EE in the P. dabryanus diet could result in a better growth rate. We hypothesized from metabolomic and transcriptomic analyses that the CP40EE10 diet might promote the growth of P. dabryanus by promoting protein synthesis, lipid metabolism, and energy production.

Lymphatic Regulation in Tissue Repair and Regeneration: Recent Advances and Future Perspective.

Lymphatic vasculature plays essential role in interstitial tissue uptake, immune cell transport and dietary lipid absorption. Increasing evidence has demonstrated the contribution of lymphangiogenesis to tissue repair and regeneration, which is associated with multiple factors such as improved tissue homeostasis, inflammation resolution, and immunomodulation effects. Meanwhile, lymphangiogenesis has the potential to regulate cell growth and proliferation through paracrine effects. Lymphatic vessels can also be important components of the stem cell niche and participate in regulating stem cell quiescency or activity. In perspective, the functions and mechanisms of lymphatic vessels in tissue repair and regeneration deserve further investigation. Novel strategies to stimulate lymphangiogenesis by using pharmacological, genetic, and lymphatic tissue engineering will be prospective to promote tissue repair and regeneration.

Impact of inner mitochondrial protein on dietary lipid metabolism and atherosclerosis progression

Impact of inner mitochondrial protein on dietary lipid metabolism and atherosclerosis progression

Effects of dietary lipid and air replenisher on the growth and lipid deposition of steelhead trout (Oncorhynchus mykiss) confined underwater

In this study, we investigated the effects of dietary lipids (DL) on the growth and lipid deposition of steelhead trout (Oncorhynchus mykiss) confined underwater using various equipment. Three diets formulated with 6%, 13%, and 20% DL (F6, F13, and F20) were fed to the fish. Fish (initial weight: 75.01 ± 1.36 g) in each dietary group were randomly cultured in three equipment groups: the control group; the submergence group, in which mesh and airstones were used to keep the experimental fish from the water surface; and the replenisher group, in which an air replenisher and airstones were utilized under the mesh. The results of this 70-day study showed that the growth and crude lipid content of the steelhead trout fish body increased significantly with increasing DL levels. No significant differences were observed in the liver among the different equipment groups, whereas the lipid contents in the muscle and the viscerosomatic index significantly decreased in the submerged group fed F20. The fitted curve analysis showed that the energy expenditure of continuous swimming and the success of replenishing the swim bladder may determine the strategy adopted by trout to cope with negative buoyancy. If the swim bladder was easily replenished, steelhead trout tended to convert excess energy into lipid stored in muscles, thereby reducing body density and maintaining neutral buoyancy. Otherwise, trout must burn lipid in their muscles to ensure energy supply to keep swimming for lift. We concluded that a high-lipid diet compensated for the loss of the energy used in resistance to negative buoyancy, and growth performance of steelhead trout improved.

Oxidized dietary lipids induce vascular inflammation and atherogenesis in post-menopausal rats: estradiol and selected antihyperlipidemic drugs restore vascular health in vivo

BackgroundThermoxidation of edible oil through deep fat frying results in the generation of several oxidized products that promote lipid peroxidation and ROS production when eaten. Consumption of thermoxidized oil in post-menopausal conditions where the estrogen level is low contributes to cardiovascular disease. This study evaluates the role of estradiol and antihyperlipidemic agents (AHD) in restoring the vascular health of ovariectomized (OVX) rats fed with thermoxidized palm oil (TPO) and thermoxidized soya oil (TSO) diets.MethodA total of 10 groups of rats (n = 6) were set up for the experiment. Group I (normal control) rats were sham handled while other groups were OVX to bring about estrogen deficient post-menopausal state. Group II (OVX only) was not treated and received normal rat chow. Groups III-X were fed with either TPO or TSO diet for 12 weeks and treated with estradiol (ETD) 0.2 mg/kg/day, atorvastatin (ATV) 10 mg/kg/day, and a fixed-dose combination of ezetimibe and ATV (EZE 3 mg/kg/day + ATV 10 mg/kg/day).ResultsPro-atherogenic lipids levels were significantly elevated in untreated TSO and TPO groups compared to OVX and sham, resulting in increased atherogenic and Coronary-risk indices. Treatment with Estradiol and AHDs significantly reduced the total cholesterol, triglycerides, low-density lipoprotein cholesterol as well as AI and CRI compared to untreated TSO and TPO groups, whereas TSO and TPO groups showed significant elevation in these parameters compared to Group I values. Moreover, aortic TNF-α levels were extremely elevated in the untreated TSO and TPO compared to Group I. TNF-α levels were significantly reduced in rats treated with AHDs and ETD. Localized oxidative stress was indicated in the aortic tissues of TSO and TPO-fed OVX rats by increased malondialdehyde and decreased glutathione, catalase, and superoxide dismutase levels. This contributed to a depletion in aortic nitric oxide. AHDs and ETD replenished the nitric oxide levels significantly. Histological evaluation of the aorta of TSO and TPO rats revealed increased peri-adventitia fat, aortic medial hypertrophy, and aortic recanalization. These pathologic changes were less seen in AHDs and ETD rats.ConclusionThis study suggests that ETD and AHDs profoundly attenuate oxidized lipid-induced vascular inflammation and atherogenesis through oxidative-stress reduction and inhibition of TNF-α signaling.

Tocopherol degradation and lipid oxidation during storage of Chenopodium quinoa

Chenopodium quinoa serves as an essential source of dietary lipids and tocopherols. The current study evaluated the stability of tocopherols and the occurrence of lipid oxidation in quinoa seeds, sprouts, and flakes during storage. Quinoa samples (seed flour, sprout flour, flakes) were stored at different temperatures (0 °C, 25 °C, 35 °C); changes in their tocopherol profile and the progress of lipid oxidation were evaluated for 180 days. Tocopherol concentration was measured using HPLC, and the degradation of major homologues was modeled using first and second order kinetics. γ- tocopherol (107–135 µg g-1 dwb) was present in highest concentration in fresh products, followed by α- tocopherol (19.3–32.4 µg g-1 dwb) and δ- tocopherol (1.6–2.3 µg g-1 dwb). Storage at 25 °C and 35 °C resulted in a drastic reduction of tocopherols; out of the three products, seed flour and flakes retained < 50 % of γ- and α- tocopherols after 180 days, while sprout flour showed better retention at both temperatures. The investigation of lipid oxidation revealed the presence of 13 volatile compounds that were formed due to oxidation of linoleic, linolenic, and oleic acids. Hexanal, a key indicator of oxidation, significantly increased at 35 °C, demonstrating that temperature during storage represents a critical factor in maintaining the quality of quinoa products.

Mitigation of cold stress in Nile tilapia (Oreochromis niloticus) through dietary lipids supplementation: a preliminary network meta-analysis.

There is a growing body of evidence suggesting that water temperature can significantly impact the dietary fatty acid requirements of Nile tilapia (Oreochromis niloticus). Therefore, this study assessed the effectiveness of different dietary lipid sources on the growth performance of Nile tilapia reared at suboptimal temperatures. A network meta-analysis was performed, including searches of PubMed and Scopus from inception to January 2022, for trials that evaluated the effects of lipid sources on cold-stressed Nile tilapia. The Bayesian hierarchical framework was used to pool and compare the effect sizes of growth parameters such as weight gain, feed intake, and feed conversion ratio (FCR). Furthermore, the surface under the cumulative ranking curve (SUCRA) was obtained to calculate the probability that each lipid source was the most effective against cold stress. All subsequent numbers refer to comparisons with diets containing only fish oil. Dietary Aurantiochytrium significantly increased weight gain (SMD = 2.00, CrI: 0.70 to 3.40). In contrast, diets containing coconut oil led to significantly lower weight gain (SMD = - 3.30, CrI: - 6.00 to - 0.63) and higher FCR (SMD = 17.0, CrI: 6.70 to 27.0). Additionally, dietary corn oil was associated with a decrease in feed intake (SMD = - 2.32, CrI: - 3.91 to - 0.80), while a combination of fish and corn oil reduced FCR (SMD = - 5.70, CrI: - 11.0 to - 0.81). In general, the analysis of SUCRA values revealed that in cold-stressed Nile tilapia, Aurantiochytrium, sunflower oil, and the combination of fish and corn oil were the most effective lipid sources for improving growth at suboptimal temperatures. The results of the current study can serve as a basis for future studies that focus on the use of dietary lipid sources to mitigate cold stress in Nile tilapia.

Physiology of lipid digestion and absorption in poultry: An updated review on the supplementation of exogenous emulsifiers in broiler diets.

Lipids are a concentrated source of energy with at least twice as much energy as the same amount of carbohydrates and protein. Dietary lipids provide a practical alternative towardincreasing the dietary energy density of feeds for high-performing modern broilers. However, the digestion and absorption of dietary lipids are much more complex than that of the other macronutrients. In addition, young birds are physiologically limited in their capacity to utilise dietary fats and oils effectively. The use of dietary emulsifiers as one of the strategies aimed at improving fat utilisation has been reported to elicit several physiological responses including improved fat digestibility and growth performance. In practical terms, this allows for the incorporation of lipids into lower-energy diets without compromising broiler performance. Such an approach may potentially lower feed costs and raise revenue gains. The current review revisits lipids and the different roles that they perform in diets and whole-body metabolism. Additional information on the process of dietary lipid digestion and absorption in poultry; and the physiological limitation brought about by age on lipid utilisation in the avian gastrointestinal tract have been discussed. Subsequently, the physiological responses resulting from the dietary supplementation of exogenous emulsifiers as a strategy for improved lipid utilisation in broiler nutrition are appraised. Suggestions of nascent areas for a better understanding of exogenous emulsifiers have been highlighted.

Nutritional and Functional New Perspectives and Potential Health Benefits of Quinoa and Chia Seeds.

Quinoa (Chenopodium quinoa Willd) and chia (Salvia hispanica) are essential traditional crops with excellent nutritional properties. Quinoa is known for its high and good quality protein content and nine essential amino acids vital for an individual's development and growth, whereas chia seeds contain high dietary fiber content, calories, lipids, minerals (calcium, magnesium, iron, phosphorus, and zinc), and vitamins (A and B complex). Chia seeds are also known for their presence of a high amount of omega-3 fatty acids. Both quinoa and chia seeds are gluten-free and provide medicinal properties due to bioactive compounds, which help combat various chronic diseases such as diabetes, obesity, cardiovascular diseases, and metabolic diseases such as cancer. Quinoa seeds possess phenolic compounds, particularly kaempferol, which can help prevent cancer. Many food products can be developed by fortifying quinoa and chia seeds in different concentrations to enhance their nutritional profile, such as extruded snacks, meat products, etc. Furthermore, it highlights the value-added products that can be developed by including quinoa and chia seeds, alone and in combination. This review focused on the recent development in quinoa and chia seeds nutritional, bioactive properties, and processing for potential human health and therapeutic applications.

George Alan Garton. 4 June 1922—13 May 2010

Alan Garton and his group at The Rowett Research Institute in Aberdeen made very significant and unique contributions to the understanding of differences in digestion, absorption and metabolism of dietary lipids (fats) and their importance to health in mammalian species, particularly farm animals, but also various aspects of lipid metabolism and health in humans. The group were the first to use commercially available gas chromatography and first, this side of the Atlantic, to employ thin-layer chromatography for lipid analyses. A special adaptation of radio-gas chromatography, developed in-house, also led to the definitive evidence that radio-labelled methylmalonyl-CoA, derived from propionic acid, particularly from grain-fed sheep, can be utilized for fatty acid synthesis. Alan always highlighted the relevance of the group's lipid research to human health and wellbeing, as seen by the role of branched-chain fatty acids in Refsum's disease and the later development of studies of the role of saturated and essential omega-3, -6 and -9 fatty acids, including conjugated linoleic acids and trans fatty acids, in human health and disease by the group.

Impacts of replacing dietary fish meal with poultry by-product meal on growth, digestive enzymes and gut microbiota, biomarkers of metabolic and immune response, and resistance to Vibrio challenge in abalone (Haliotis discus hannai)

A 110-day feeding trial was carried out to investigate the impacts of replacing dietary fish meal (FM) with poultry by-product meal (PBM) on growth performance, digestion, gut microbiota, immunity, and disease resistance of abalone Haliotis discus hannai (initial weight: 22.53 ± 0.21 g). The control diet (PBM0) without PBM contained 20% of FM. Based on PBM0, the other four isonitrogenous (about 30% of crude protein) and isolipidic (about 4% of crude lipid) diets were formulated replacing 25, 50, 75 and 100% of FM by PBM (designated as PBM25, PBM50, PBM75 and PBM100), respectively. Every diet was fed to triplicate cages (40 × 35 × 70 cm) with 60 abalones per cage. At the end of the feeding trial, abalones were starved for 3 days and anesthetized with 5% ethanol before sampling. Results indicated that biomass gain of the abalone fed PBM75 (916.71 g) and PBM100 (659.93 g) was significantly lower than that of the abalone fed PBM0 (1222.51 g). Feed conversion ratio of the abalone fed PBM50 (2.93), PBM75 (2.89) and PBM100 (4.04) were significantly higher than that of the abalone fed PBM0 (2.37). The muscle crude protein content of the abalone fed PBM100 (16.01% wet weight) was significantly lower than that of the abalone fed PBM0 (16.67% wet weight). Activities of trypsin and α-amylase in the digestive gland in the PBM100 group (309.21 and 0.064 U/mg prot., respectively) were significantly lower than the PBM0 group (463.57 and 0.078 U/mg prot., respectively). When the substitution levels rose over 50%, expressions of phosphatidylinositol 3 kinase, threonine kinase/protein B, mammalian target of rapamycin and eukaryotic translation initiation factor 4E in muscle were significantly reduced, while 4E binding protein was significantly increased. The most abundant phyla across the abalones' gut were Proteobacteria and Firmicutes. Catalase (from 2.90 to 1.69 U/mL), superoxide dismutase (from 98.93 to 74.39 U/mL), lysozyme (from 345.86 to 247.81 U/mL) and acid phosphatase (from 21.68 to 15.71 U/100 mL) activities in cell-free hemolymph were markedly decreased with substitution level increasing. When the replacement levels rose over 50%, myeloid differentiation primary response gene 88 and tumor necrosis factor α expressions in the digestive gland were markedly upregulated, while β-defensin and mytimacin 6 expressions were markedly downregulated. The cumulative mortality of abalone challenged with Vibrio parahaemolyticus (1.0 × 107 CFU/mL) for 7 days was remarkably increased in the PBM50 (83.49%), PBM75 (89.56%) and PBM100 (100%) groups compared with the PBM0 group (58.82%). In conclusion, 50% of dietary FM could be replaced by PBM without influencing abalone growth. When the replacement level was over 50%, the growth and immunity of abalone were decreased, and the mTOR pathway was negatively affected.

Dietary protein, lipid and insect meal on growth, plasma biochemistry and hepatic immune expression of lake whitefish (Coregonus clupeaformis)

Studies are lacking that investigate the dietary nutrient requirements of lake whitefish (Coregonus clupeaformis), a newly farmed fish species in Ontario, Canada. Dietary levels of protein and lipid must be optimized to ensure high growth performance for the commercial success of this species. Additionally, the inclusion of insect meal in the diet may improve growth and immune response. The objective of this study was to evaluate the effects of dietary protein:lipid ratios and insect meal as a feed additive on the growth performance and hepatic immune function of juvenile lake whitefish (301 ± 10 g). A 16-week (112 day) trial was performed with five diets including a commercial control diet (BCC), and four experimental diets with high or low levels of protein (54 and 48%, respectively) and lipid (18 and 12%, respectively). The high protein dietary groups contained 5% of full-fat black soldier fly larvae (Hermetia illucens). Fish weights, viscera, liver, and blood were collected for further analysis. Specific growth rate, thermal growth coefficient and weight gain were significantly higher in fish fed with the BCC and high protein high lipid (HPHL) diets. However, viscerosomatic index was found to be significantly higher in fish fed the BCC diet, thus HPHL is more optimal for non-visceral weight gain. Higher levels of plasma phosphorus, aspartate aminotransferase and potassium indicated poor growth and stress in fish fed low lipid diets. Relative expression of HSP70, involved in cellular repair, was significantly downregulated in fish fed high lipid diets, and no effects were found on the expression of innate immune and oxidative stress genes. Also, IL8 (CXCL8) and catalase were upregulated (non-significant) in fish fed the HPHL diet with the largest weight gain. No effects of insects were found on growth, plasma biochemistry or gene expression, which suggests 5% dietary inclusion was too low. Overall, we recommend a HPHL diet for the cultivation of lake whitefish based on improved growth performance, low viscera weight, improved plasma biochemistry and downregulation of cellular repair genes.