Dietary Excess Research Articles

Research Progress of Dietary Habits and the Hypertension

Chronic disease (include hypertension) is a kind of lifelong disease with serious bodily function damage, which can only be controlled, but cannot be cured. The morbidity of complications is high, and with high disability rate and mortality rate, the health management of chronic disease has become an important public health problem in China. At present, there are abundant drugs for the treatment of hypertension on the market, but they may only regulate a certain aspect, so that the blood pressure control effect is not ideal, and increasing the dose can hardly reduce the extra blood pressure but will increase the risk of adverse reactions. There are many factors that cause the high blood pressure, and they are closely related to dietary habits. In order to ensure that people can have a reasonable diet and good health, the article makes a detailed study on the dietary habits (such as the high salt and low potassium, high fat, excessive diet) and hypertension.

Analysis on Syndrome and Treatment of Shaoyin Cold Type Acute Tonsillitis

Acute tonsillitis belongs to the category of "milk moth" in traditional Chinese medicine. TCM clinical syndrome differentiation is often divided into two types: wind-heat pathogens affecting the lung meridian and dietary excess leading to heat accumulation in the lungs and stomach. The author's clinical practice found that some acute tonsillitis patients are often manifested as Shaoyin cold type, and the syndrome and treatment of general acute tonsillitis are totally different. "Throat Science Family Instructions" put forward the specific treatment of pharyngeal pain is Shaoyin typhoid type, and the prescription is Banxia Gangui Decoction, which is composed of eight kinds of drugs: Guizhi, Banxia, Fuling, Jiegeng, Yiyiren, Buguzhi, Ganjiang, and Zexie. It is traditionally used to treat symptoms such as sore throat, diarrhea, deep and thin pulse, and white tongue coating without thirst. Combining theory and clinical experience, the author reports successful clinical outcomes using this decoction to treat acute tonsillitis of the Shaoyin cold type. This paper aims to analyze the internal connection between Banxia Gangui Decoction and Shaoyin cold type acute tonsillitis. The paper outlines the symptom characteristics, etiology and pathogenesis, treatment based on syndrome differentiation, differential diagnosis of this condition, along with the therapeutic rationale for Banxia Gangui Decoction. A case study is included.

Impaired Upper Airway Muscle Function with Excessive or Deficient Dietary Intake of Selenium in Rats.

Obstructive sleep apnoea (OSA) involves impaired upper airway muscle function and is linked to several pathologies including systemic hypertension, daytime somnolence and cognitive decline. Selenium is an essential micronutrient that exerts many of its effects through selenoproteins. Evidence indicates that either deficient or excessive dietary selenium intake can result in impaired muscle function, termed nutritional myopathy. To investigate the effects of selenium on an upper airway muscle, the sternohyoid, rats were fed on diets containing deficient, normal (0.5 ppm sodium selenite) or excessive (5 ppm selenite) selenium for a period of two weeks. Sternohyoid contractile function was assessed ex vivo. Serum selenium levels and activity of the glutathione antioxidant system were determined by biochemical assays. The abundance of three key muscle selenoproteins (selenoproteins -N, -S and -W (SELENON, SELENOS and SELENOW)) in sternohyoid muscle were quantified by immunoblotting. Levels of these selenoproteins were also compared between rats exposed to chronic intermittent hypoxia, a model of OSA, and sham treated animals. Although having no detectable effect on selected organ masses and whole-body weight, either selenium-deficient or -excessive diets severely impaired sternohyoid contractile function. These changes did not involve altered fibre size distribution. These dietary interventions resulted in corresponding changes in serum selenium concentrations but did not alter the activity of glutathione-dependent antioxidant systems in sternohyoid muscle. Excess dietary selenium increased the abundance of SELENOW protein in sternohyoid muscles but had no effect on SELENON or SELENOS. In contrast, chronic intermittent hypoxia increased SELENON, decreased SELENOW and had no significant effect on SELENOS in sternohyoid muscle. These findings indicate that two-week exposure to selenium-deficient or -excessive diets drastically impaired upper airway muscle function. In the sternohyoid, SELENON, SELENOS and SELENOW proteins show distinct alterations in level following exposure to different dietary selenium intakes, or to chronic intermittent hypoxia. Understanding how alterations in Se and selenoproteins impact sternohyoid muscle function has the potential to be translated into new therapies for prevention or treatment of OSA.

Copper exposure promotes ferroptosis of chicken (Gallus gallus) kidney cells and causes kidney injury

PurposeWhile copper (Cu) is essential for biological organisms, excessive Cu can be harmful. Ferroptosis is a programmed cell death pathway, but the role of ferroptosis in renal injury induced by Cu is limited. The aim of this study was to investigate the role of ferroptosis in kidney injury in chickens and the molecular mechanism by which Cu promotes renal ferroptosis. Materials and methodsChicken were subjected to Cu treatment by artificially adding excess Cu to the basal diet (the Cu concentration in the diet was supplemented to 110–330 mg/kg), and the impact on kidney fibrosis, tissue structure, and ferroptosis-related molecular markers was studied. Then, the expression levels of genes and proteins related to ferroptosis, iron metabolism and ferroautophagy were detected to explore the promoting effect of Cu on ferroptosis in chicken kidney. Main findingsCu treatment resulted in significant fibrosis and tissue structure damage in chicken kidneys. Molecular analysis revealed a significant upregulation of LC3Ⅱ, P62, ATG5, and NCOA4, along with a decrease in FTH1 and FTL protein levels. Additionally, crucial markers of ferroptosis, including the loss of GPX4, SLC7A11, and FSP1, and an increase in PTGS2 and ACSL4 protein levels, were observed in chicken kidneys after Cu exposure. ConclusionOur study showed that dietary Cu excess caused kidney injury in brochickens and exhibited ferroptosis-related features, including lipid peroxidation, reduction of ferritin, and downregulation of FSP1 and GPX4. These results indicate that excess Cu can induce renal ferroptosis and lead to kidney injury in chickens. This study highlights the complex interplay between Cu ions and ferroptosis in the context of renal injury and provides a new perspective for understanding the mechanism of Cu-induced renal injury.

The Metabolic and Endocrine Effects of a 12-Week Allulose-Rich Diet

The global rise in type 2 diabetes (T2D) and obesity necessitates innovative dietary interventions. This study investigates the effects of allulose, a rare sugar shown to reduce blood glucose, in a rat model of diet-induced obesity and T2D. Over 12 weeks, we hypothesized that allulose supplementation would improve body weight, insulin sensitivity, and glycemic control. Our results showed that allulose mitigated the adverse effects of high-fat, high-sugar diets, including reduced body weight gain and improved insulin resistance. The allulose group exhibited lower food consumption and increased levels of glucagon-like peptide-1 (GLP-1), enhancing glucose regulation and appetite control. Additionally, allulose prevented liver triglyceride accumulation and promoted mitochondrial uncoupling in adipose tissue. These findings suggest that allulose supplementation can improve metabolic health markers, making it a promising dietary component for managing obesity and T2D. Further research is needed to explore the long-term benefits and mechanisms of allulose in metabolic disease prevention and management. This study supports the potential of allulose as a safe and effective intervention for improving metabolic health in the context of dietary excess.

Zinc nutrition at first feeding imprints a programming effect on growth and hepatic lipid metabolism in juvenile rainbow trout

The objective of this study was to determine whether supplementation with deficient zinc (Zn) or/and excess copper (Cu) in the first-feeding diet of rainbow trout fry influenced the growth and physiological regulation of juvenile fish subjected to similar challenge feeds again. The feeding trial lasted for 24 weeks and had three phases. In phase I, rainbow trout fry was treated with one of four different plant-based feeds containing or exempting Zn or Cu supplementation (coded Zn-Cu-, Zn+Cu-, Zn-Cu+, Zn+Cu+) for 6 weeks. Thereafter, all groups were fed a common commercial feed for the next 12 weeks (phase II). In phase III, all groups were fed with the “challenge diet” for another 6 weeks which was the basal diet (Zn-Cu-) except that the vegetable oils were replaced by fish oil. The results demonstrated that fish fed a Zn+ diet at first feeding increased larval growth (phase-I), but it had no effect on growth at the end of phase II when fed commercial feeds. In phase III, when re-introduced to a Zn- challenge diet, the feed intake and growth of juveniles with Zn+ history significantly increased, with reduced feed efficiency. Fish growth was neither influenced by dietary Cu levels or by the dietary Cu history in any of the growth phases, but dietary Cu excess (Cu+) reduced body lipid and energy content in fry. In phase I, lower whole-body Zn concentration was observed in the fry of Zn- group compared to Zn+, while the contrary was observed in the juvenile fish as affected by dietary Zn history (phase III). In addition, Zn- dietary history showed increased levels of PUFA and higher mRNA expression of fatty acid biosynthesis genes in the liver. To conclude, early-stage dietary Zn+ history improved growth of juvenile rainbow trout, while dietary Zn- history exhibited signs of improved fatty acid biosynthesis capacity in the liver.

Nanopore sequencing demonstrates the roles of spermatozoal DNA N6-methyladenine in mediating transgenerational lipid metabolism disorder induced by excessive folate consumpton

Increased consumption of folic acid is prevalent due to its beneficial effects, but growing evidence emphasizes the side effects pointing to excessive dietary folate intake. The effects of excessive paternal folic acid consumption on offspring and its transgenerational inheritance mechanism have not been elucidated. We hypothesize that excessive folic acid consumption will alter sperm DNA N6-methyladenine (6mA) and 5-methylcytosine (5mC) methylation and heritably influence offspring metabolic homeostasis. Here, we fed roosters either folic acid-control or folic acid-excess diet throughout life. Paternal chronic folic acid excessive supplementation increased hepatic lipogenesis and lipid accumulation but reduced lipolysis both in the roosters and their offspring, which was further confirmed to be induced by one-carbon metabolism inhibition and gene expression alteration associated with the Peroxisome proliferator-activated receptor pathway. Based on the spermatozoal genome-wide DNA methylome identified by Nanopore sequencing, multi-omics association analysis of spermatozoal and hepatic DNA methylome, transcriptome, and metabolome suggested that differential spermatozoal DNA 6mA and 5mC methylation could be involved in regulating lipid metabolism-related gene expression in offspring chickens. This model suggests that sperm DNA N6-methyladenine and 5-methylcytosine methylation were involved in epigenetic transmission and that paternal dietary excess folic acid leads to hepatic lipid accumulation in offspring.

Excessive caffeine use and its consequence on mental illness: a cross-sectional study in Indonesia

Abstract Coffee is one of the most popular beverages globally. People often drink coffee for its caffeine content and its stimulating effects. However, excessive coffee consumption, like any other dietary or behavioral excess, can negatively affect physical and mental health. This study investigates the association between excessive coffee use and mental problems. A cross-sectional study was conducted among 392 respondents in Aceh, Indonesia. Excessive coffee use was measured using a questionnaire, while mental illness was screened using DASS-21. Other sociodemographic variables were also examined and treated as possible predictors. The prevalence of excessive coffee use (>400 mg per day) was 15.6%. The prevalence of depression was 23.2%, anxiety was 40.8%, and stress was 12.8%. The amount of caffeine consumption was correlated with depression (rs = 0.11, p = 0.01), anxiety (rs = 0.13, p = 0.001), and stress (rs = 0.14, p = 0.001), indicating that as the amount of coffee consumption increased, the score of mental problem also increased. The study established a noteworthy correlation between caffeine intake and mental health, suggesting that as coffee consumption increased, so did the scores for depression, anxiety, and stress. These findings underscore the importance of considering coffee consumption habits and their potential impact on mental well-being in clinical and public health contexts.

Electrophysiological Characteristics of Inhibitive Control for Adults with Different Physiological or Psychological Obesity.

Individuals exhibiting high scores on the fatness subscale of the negative-physical-self scale (NPSS-F) are characterized by heightened preoccupation with body fat accompanied by negative body image perceptions, often leading to excessive dieting behaviors. This demographic constitutes a considerable segment of the populace in China, even among those who are not obese. Nonetheless, scant empirical inquiries have delved into the behavioral and neurophysiological profiles of individuals possessing a healthy body mass index (BMI) alongside elevated NPSS-F scores. This study employed an experimental paradigm integrating go/no-go and one-back tasks to assess inhibitory control and working memory capacities concerning food-related stimuli across three adult cohorts: those with normal weight and low NPSS-F scores, those with normal weight and high NPSS-F scores, and individuals classified as obese. Experimental stimuli comprised high- and low-caloric-food pictures with concurrent electroencephalogram (EEG) and photoplethysmogram (PPG) recordings. Individuals characterized by high NPSS-F scores and normal weight exhibited distinctive electrophysiological responses compared to the other two cohorts, evident in event-related potential (ERP) components, theta and alpha band oscillations, and heart rate variability (HRV) patterns. In essence, the findings underscore alterations in electrophysiological reactivity among individuals possessing high NPSS-F scores and a healthy BMI in the context of food-related stimuli, underscoring the necessity for increased attention to this demographic alongside individuals affected by obesity.

Personalized Health-Centric Food Recommendation System

People nowadays suffer from a wide range of acute and chronic conditions. It is challenging to properly prescribe a diet in modern world. When a body does not obtain enough nutrients, it might develop nutritional disorders, which can cause a number of different health issues. Chronic conditions including hypertension, cardiovascular disease, diabetes, and others can be caused on by dietary deficiencies or excesses of particular nutrients as well as eating disorders. Primary root cause of these conditions is typically an inadequate or improper diet. We propose a diet suggestion system in this study that takes the user’s physics details and symptoms into account. Machine learning methods (KNN) are used in this study. The system predicts the proposed meal plan for a user by leveraging its training data, which comprises daily calorie requirements and food consumption patterns. According to the evaluation of the dataset, the suggested model outperforms existing diet recommendation systems. Analyzing the user’s dietary and nutritional requirements, the suggested system generates a prediction about the meal plan. It could result in a customized food plan for each person and promote better health

THE ROLE OF THE MEDITERRANEAN DIET IN PREVENTING MALE INFERTILITY: AN INTERVIEW WITH DR. MIGUEL FORNES (PT-BR VERSION)

Introduction: The interview with Dr. Miguel Fornes, a researcher at CONICET and director of the Andrological Research Laboratory of Mendoza, Argentina, discusses his research on the effects of a high-fat diet on male reproductive health and infertility related to hypercholesterolemia. Objectives: Dr. Fornes' primary research objective is to investigate how dietary fat excess and the consequent increase in cholesterol affect sperm physiology and function, specifically concerning capacitation, acrosomal reaction, and egg fertilization. Results: Studies conducted by Dr. Fornes' laboratory have revealed that a high-fat diet promotes hypercholesterolemia, which in turn increases cholesterol levels in sperm. This cholesterol increase interferes with sperm's normal physiology, hindering proper acrosomal reaction, a crucial step for fertilization. Discussion: The importance of adopting a healthy lifestyle, including a balanced diet such as the Mediterranean diet rich in olive oil, fruits, and vegetables, was emphasized. He highlights the benefits of olive oil, which contains antioxidant molecules that help protect cells from oxidative stress caused by excess fat. Conclusion: Dr. Fornes' research and his team have significant implications for clinical interventions and dietary guidelines to improve male reproductive health. Their results reinforce the importance of a healthy and balanced diet, especially rich in olive oil, to prevent hypercholesterolemia and maintain normal sperm function, thereby contributing to male fertility.

Association between dietary phosphate intake and skeletal muscle energetics in adults without cardiovascular disease.

Highly bioavailable inorganic phosphate (Pi) is present in large quantities in the typical Western diet and represents a large fraction of total phosphate intake. Dietary Pi excess induces exercise intolerance and skeletal muscle mitochondrial dysfunction in normal mice. However, the relevance of this to humans remains unknown. The study was conducted on 13 individuals without a history of cardiopulmonary disease (46% female, 15% Black participants) enrolled in the pilot-phase of the Dallas Heart and Mind Study. Total dietary phosphate was estimated from 24-h dietary recall (ASA24). Muscle ATP synthesis was measured at rest, and phosphocreatinine (PCr) dynamics was measured during plantar flexion exercise using 7-T 31P magnetic resonance (MR) spectroscopy in the calf muscle. Correlation was assessed between dietary phosphate intake normalized to total caloric intake, resting ATP synthesis, and PCr depletion during exercise. Higher dietary phosphate intake was associated with lower resting ATP synthesis (r = -0.62, P = 0.03), and with higher levels of PCr depletion during plantar flexion exercise relative to the resting period (r = -0.72; P = 0.004). These associations remain significant after adjustment for age and estimated glomerular filtration rate (both P < 0.05). High dietary phosphate intake was also associated with lower serum Klotho levels, and Klotho levels are in turn associated with PCr depletion and higher ADP accumulation post exercise. Our study suggests that higher dietary phosphate is associated with reduced skeletal muscle mitochondrial function at rest and exercise in humans providing new insight into potential mechanisms linking the Western diet to impaired energy metabolism.NEW & NOTEWORTHY This is the first translational research study directly demonstrating the adverse effects of dietary phosphate on muscle energy metabolism in humans. Importantly, our data show that dietary phosphate is associated with impaired muscle ATP synthesis at rest and during exercise, independent of age and renal function. This is a new biologic paradigm with significant clinical dietary implications.

A look at duodenal mucosal resurfacing: Rationale for targeting the duodenum in type 2 diabetes.

Affecting 5%-10% of the world population, type 2 diabetes (T2DM) is firmly established as one of the major health burdens of modern society. People with T2DM require long-term therapies to reduce blood glucose, an approach that can mitigate the vascular complications. However, fewer than half of those living with T2DM reach their glycaemic targets despite the availability of multiple oral and injectable medications. Adherence and access to medications are major barriers contributing to suboptimal diabetes treatment. The gastrointestinal tract has recently emerged as a target for treating T2DM and altering the underlying disease course. Preclinical and clinical analyses have elucidated changes in the mucosal layer of the duodenum potentially caused by dietary excess and obesity, which seem to be prevalent among individuals with metabolic disease. Supporting these findings, gastric bypass, a surgical procedure which removes the duodenum from the intestinal nutrient flow, has remarkable effects that improve, and often cause remission of, diabetes. From this perspective, we explore the rationale for targeting the duodenum with duodenal mucosal resurfacing (DMR). We examine the underlying physiology of the duodenum and its emerging role in T2DM pathogenesis, the rationale for targeting the duodenum by DMR as a potential treatment for T2DM, and current data surrounding DMR. Importantly, DMR has been demonstrated to change mucosal abnormalities common in those with obesity and diabetes. Given the multifactorial aetiology of T2DM, understanding proximate contributors to disease pathogenesis opens the door to rethinking therapeutic approaches to T2DM, from symptom management toward disease modification.

The Risks of Premature and Excessive Diet After Peroral Endoscopic Myotomy.

The Risks of Premature and Excessive Diet After Peroral Endoscopic Myotomy.

Excess dietary Lys reduces feed intake, stimulates jejunal CCK secretion and alters essential and non-essential blood AA profile in pigs

BackgroundCommercial diets are frequently formulated to meet or exceed nutrient levels including those of limiting essential amino acids (AA) covering potential individual variations within the herd. However, the provision of dietary excess of AA, such as Lys, may lead to reduced appetite and growth in pigs. The mechanisms modulating these responses have not been extensively investigated. This study evaluated the effect of Lys dietary excesses on performance and satiety biomarkers in post weaning pigs.MethodsTwenty-four pigs aged 21 d and weighing 6.81 ± 0.12 kg (mean ± SEM) were individually housed and offered 1 of 4 dietary treatments for 3 weeks: a diet containing a standardized ileal digestible Lys reaching 100% (T0), 120% (T1), 150% (T2) or 200% (T3) of the NRC (2012) requirements. At the end of the experiment, blood samples from the cephalic vein of the T0 and T3 groups were obtained for AA analysis. In addition, primary intestinal cultures from T0 pigs were used, following their humane killing, to evaluate the effect of Lys on gut hormone secretion and AA sensors gene expression under ex vivo conditions.ResultsFeed intake was linearly reduced (P < 0.001) and the weight gain to feed ratio reduced (P < 0.10) with increased dietary levels of Lys during the third- and first-week post weaning, respectively. Cholecystokinin concentration (P < 0.05) and the metabotropic glutamate receptor 1 and the solute carrier family 7 member 2 (P < 0.10) gene expression was enhanced in proximal jejunum tissues incubated with Lys at 20 mmol/L when compared to the control (Lys 0 mmol/L). Plasma Lys and Glu (P < 0.05) concentration increased in the T3 compared to T0 pigs. In contrast, plasma levels of His, Val, Thr, Leu (P < 0.05) and Gln (P < 0.10) were lower in T3 than T0 pigs.ConclusionThe present results confirm that excess dietary Lys inhibits hunger in pigs. Moreover, the results provide evidence of pre- and post-absorptive mechanisms modulating these responses. Lys dietary excesses should be narrowed, when possible, to avoid negative effects of the AA on appetite in pigs.

The impact of excessive dieting on eating disorders in adolescent women: a literature review

Purpose This paper aims to in the modern world, possessing an attractive appearance is often considered a highly valued attribute. As such, the perceptions and satisfaction with one’s body are shaped by dominant cultural norms. Adolescents, women in particular, who are heavily influenced by media representations, may tend to have a distorted body image (BI), including adopting extreme dieting methods. This study reviews the adverse effects of excessive weight loss associated with this. Design/methodology/approach The authors searched journals and the internet for relevant literature using the keywords “eating disorders”, “body image” and “weight stigma”. In the case study field, they added papers that considered “nutrition” to identify the link between dieting behaviour and nutrition. From these reviews, the authors ultimately selected 190 articles that appeared to meet their research objectives. The papers cover a range of studies published between 1995 and 2023. Findings Among adolescent girls and young women in their early 20s, there is a social media-driven culture of being extremely thin and petite. Weight stigma puts more pressure on them and makes strange behaviours like pro-ana syndrome a part of the culture. The authors have seen that modern BI standards leave young women vulnerable to eating disorders caused by excessive dieting. Originality/value Adolescence is a time of continuous growth, so balanced nutrition is essential. However, biased societal standards of beauty can push adolescent girls who are sensitive to external gaze into excessive dieting and make eating disorders a culture. This review provides a perspective on the behaviours that should be pursued for a healthy BI.

Review of impacts of using body mass index as a screening tool in school children: A scoping review.

Body mass index (BMI) is used to screen for under- or overweight. However, it is unknown whether BMI-screening in school children reduces the incidence of overweight and obesity. We aimed to summarise the knowledge on positive and negative consequences on BMI and weight-related perceptions and behaviours of BMI-screening followed by an intervention to prevent and/or address obesity. We performed a systematic literature search, April 2023, using the PubMed, ScienceDirect and Google Scholar databases. We included randomised controlled trials and observational studies, including children aged 6 to 18 years and examining BMI-screening in a school setting. The primary outcome measures were BMI and weight status. Secondary outcome measures were weight-related perceptions and behaviours. Three studies met the inclusion criteria; two randomised controlled trials and one observational study. All studies used BMI-screening followed by sending report cards to the parents. None of the studies found a change in BMI z-scores in school children. Screening could cause transitory body-dissatisfaction and increase peer weight talk, yet potentially reduce problematic weight behaviours like skipping meals or excessive dieting. Reporting BMI to parents may influence their perception of their children's weight status, but does not change behaviour. The sparse evidence available suggested that BMI screening followed by sending report cards to parents does not change BMI z-scores among children in primary, middle and high school. Studies using appropriate evidence-based weight management interventions as a follow-up are lacking. Such studies may improve future prevention, detection and treatment of weight-related issues.

Study on Serum Calcium, Magnesium and Uric acid Levels in Preeclamptic and Healthy Pregnant Women - A Comparative Study

Background &amp; Objective: Pre-eclampsia is the most common medical complication of pregnancy associated with increased maternal and infant mortality and morbidity. Various dietary deficiencies or excesses have previously been linked to preeclampsia. Many studies have documented that the changes in the concentration of minerals like calcium, magnesium, and uric acid have a link to the etiopathogenesis of preeclampsia. The present study was undertaken to find the associations of serum calcium, magnesium, and uric acid with preeclampsia. Methods: This cross-sectional comparative study was carried out in the Department of Physiology in collaboration with the Department of Biochemistry, Rajshahi Medical College, Rajshahi over a period of one year between July 2018 to June 2019. Pre-eclamptic and healthy pregnant women aged 18-35 years with single-tone pregnancies having agestational age of 28-32 weeks were the study population. While preeclamptic pregnant women were included as cases, healthy pregnant women were included as controls. A total of 100 cases (pre-eclamptic women) and an equalnumber of controls (healthy pregnant women) were included. Serum calcium, magnesium, and uric acid levels of the subjects were compared between the case and the control groups to evaluate their association with preeclampsia. Result: The findings of the study revealed that cases were older and heavier than the controls. However, both groups were alike in terms of gestational age. Both systolic and diastolic blood pressures were much higher in the case group than those in the control group. The serum calcium was significantly decreased in the case group than that in the control group (p &lt; 0.001). The serum magnesium was also significantly dropped in the case group compared to the control group (p &lt; 0.001). However, the serum uric acid was dramatically raised in the former group than that in the latter group (p &lt; 0.001). Conclusion: The study concluded that serum calcium and magnesium levels are significantly decreased and serum uricacid levels significantly increased in preeclamptic women. Ibrahim Card Med J 2022; 12 (2): 27-31

Association between diet soft drink consumption and metabolic dysfunction-associated steatotic liver disease: findings from the NHANES

BackgroundLifestyle change plays a crucial role in the prevention and treatment of metabolic dysfunction-associated steatotic liver disease (MASLD). In recent years, diet soft drinks that emphasize “zero sugar and zero calories” have become all the rage, but whether diet soft drink consumption is associated with MASLD is not clear.MethodsThis study included data from the National Health and Nutrition Examination Surveys (NHANES) in 2003–2006. The assessment of MASLD status primarily relied on the Fatty Liver Index (FLI). Weighted multiple Logistic regression models were constructed to evaluate the association between diet soft drink consumption and MASLD. Additionally, mediation analysis was performed to examine the mediating effect of body mass index (BMI).ResultsA total of 2,378 participants were included in the study, among which 1,089 individuals had MASLD, and the weighted prevalence rate was 43.64%. After adjusting for variables related to demographic, lifestyle, and metabolic syndrome, excessive diet soft drink consumption (the “always” frequency) remained significantly associated with the occurrence of MASLD (OR = 1.98, 95%CI = 1.36–2.89, P = 0.003). It was estimated that 84.7% of the total association between diet soft drink consumption and MASLD was mediated by BMI (P < 0.001).ConclusionsExcessive diet soft drink consumption was associated with the occurrence of MASLD. BMI may play a mediating role in the association between diet soft drink consumption and MASLD.

Effects of dietary supplementation with manganese dioxide nanoparticles on growth, Mn metabolism and kidney health of yellow catfish Pelteobagrus fulvidraco

The present study was performed to investigate yellow catfish manganese (Mn) requirement with Mn dioxide nanoparticles (MnO2NPs) as Mn source, and to evaluate effects of dietary Mn on growth performance, Mn metabolism and kidney health. Using MnO2NPs as the Mn sources, six experimental diets were formulated with dietary Mn levels at 1.67, 4.35, 7.37, 13.63, 26.06 and 50.37 mg Mn/kg diet, respectively, and fed to yellow catfish for 8 weeks. Yellow catfish growth performance and feed intake were the highest in the group of 13.63 mg Mn/kg diet, and the lowest in the group of 1.67 mg Mn/kg diet; and the feed conversion rate showed opposite trend with growth performance. Dietary Mn addition increased Mn content in the whole body, kidney and serum. The mRNA abundances of dmt1, zip8 and znt10 increased with the increase of dietary Mn level. Yellow catfish fed high dietary MnO2NPs level had higher mRNA abundances of dynamin1, dynamin2, cav1, cltc and eps15. Dietary Mn excess damaged the kidney histology. Yellow catfish fed the 13.63 mg Mn/kg diet had higher T-AOC, T-SOD, Mn-SOD, CAT activities, and mRNA levels of sod1, sod2 and cat (antioxidant response), but lower mRNA abundances of grp78, ire1, perk and atf4 (endoplasmic reticulum stress), tnfα, il1β and il6 (inflammatory response). The broken-line analysis of WG and the linear regression of whole-body Mn retention against dietary Mn levels showed that optimal Mn requirement was 12.57 mg Mn/kg diet and 8.33 mg Mn/kg diet, respectively, with MnO2NPs as Mn source. For the first time, we explored the effects of dietary MnO2 nanoparticles on growth performance, Mn uptake and metabolism, antioxidant responses, endoplasmic reticulum stress and inflammatory responses of the kidney, and determined the optimal dietary Mn requirement of yellow catfish fed MnO2 nanoparticles-added diet.