Dietary Energetics Research Articles

Influence of protein and energy level in finishing diets for feedlot hair lambs: growth performance, dietary energetics and carcass characteristics.

Forty-eight Pelibuey×Katahdin male intact lambs (23.87±2.84 kg) were used in an 84-d feeding trial, with six pens per treatment in a 2×2 factorial design arrangement. The aim of the study was to evaluate the interaction of two dietary energy levels (3.05 and 2.83 Mcal/kg ME) and two dietary protein levels (17.5% and 14.5%) on growth performance, dietary energetics and carcass traits. The dietary treatments used were: i) High protein-high energy (HP-HE); ii) High protein-low energy (HP-LE); iii) Low protein-high energy (LP-HE), and iv) Low protein-low energy (LP-LE). With a high-energy level, dry matter intake (DMI) values were 6.1% lower in the low-protein diets, while with low-energy, the DMI values did not differ between the dietary protein levels. Energy levels did not influence the final weight and average daily gain (ADG), but resulted in lower DMI values and higher gain efficiencies. No effects of protein level were detected on growth performance. The observed dietary net energy (NE) ratio and observed DMI were closer than expected in all treatments and were not affected by the different treatments. There was an interaction (p<0.03) between energy and protein level for kidney-pelvic and heart fat (KPH), KPH was higher in lambs fed high energy and high protein diet but not in high energy and low protein diet. The KPH was increased (20.2%, p = 0.01) in high-energy diets, while fat thickness was increased (21.7%, p = 0.02) in high-protein diets. Therefore, it is concluded that dietary energy levels play a more important role in feed efficiency than protein levels in finishing lambs with a high-energy diet (>2.80 Mcal/kg ME). Providing a level of protein above 14.5% does not improves growth-performance, dietary energetics or carcass dressing percentage.

Influence of Ionophore Supplementation on Growth Performance, Dietary Energetics and Carcass Characteristics in Finishing Cattle during Period of Heat Stress.

Forty-eight crossbred heifers (378.1±18 kg) were used in a 56-d feeding trial (four pens per treatment in a randomised complete block design) to evaluate the influence of ionophore supplementation on growth performance, dietary energetics and carcass characteristics in finishing cattle during a period of heat stress. Heifers were fed a diet based on steam-flaked corn (2.22 Mcal NEm/kg) with and without an ionophore. Treatments were: i) control, no ionophore; ii) 30 mg/kg monensin sodium (RUM30); iii) 20 mg/kg lasalocid sodium (BOV20), and iv) 30 mg/kg lasalocid sodium (BOV30). Both dry matter intake (DMI) and climatic variables were measured daily and the temperature humidity index (THI) was estimated. The maximum THI during the study averaged 93, while the minimum was 70 (THI average = 79.2±2.3). Compared to controls, monensin supplementation did not influence average daily gain, the estimated NE value of the diet, or observed-to-expected DMI, but tended (p = 0.07) to increase (4.8%) gain to feed. Compared to controls, the group fed BOV30 increased (p≤0.03) daily gain (11.8%), gain to feed (8.3%), net energy of the diet (5%), and observed-to-expected DMI (5.2%). Daily weight gain was greater (7.6%, p = 0.05) for heifers fed BOV30 than for heifers fed MON30. Otherwise, differences between the two treatments in DMI, gain to feed, and dietary NE were not statistically significant (p>0.11). Plotting weekly intakes versus THI, observed intake of controls was greater (p<0.05) at THI values ≤77 than ionophore groups. When THI values were greater than 79, DMI of control and MON30 were not different (p = 0.42), although less than that of groups fed lasalocid (p = 0.04). Variation in energy intake was lower (p>0.05) in the ionophores group (CV = 1.7%) than in the control group (CV = 4.5%). Inclusion of ionophores in the diet resulted in relatively minor changes in carcass characteristics. It is concluded that ionophore supplementation did not exacerbate the decline of DM intake in heat-stressed cattle fed a high-energy finishing diet; on the contrary, it stabilised feed intake and favoured feed efficiency. Ionophore supplementation reduced estimated maintenance coefficients around 10% in finishing cattle during a period of heat stress. This effect was greatest for heifers supplemented with 30 mg lasalocid/kg of diet.

Influence of forage NDF level, source and pelletizing on growth performance, dietary energetics, and characteristics of digestive function for feedlot cattle

One hundred sixty Holstein steers (222±1kg) were used in a 264-d growing-finishing trial to evaluate the influence of forage aNDFom level (40 vs 80g/kg DM) and source [ground forage (alfalfa, sudangrass, and rice straw) or pelletized rice straw] on growth performance. Forage source did not influence (P>0.10) gain efficiency or dietary net energy (NE). Daily weight gain (ADG) and dry matter intake (DMI) were similar (P>0.10) for alfalfa, sudangrass and ground rice straw. Pelletizing rice straw depressed ADG (P<0.01) and DMI (P<0.01). Increasing forage aNDFom decreased gain efficiency (P=0.09), and dietary NE (P=0.04). Four Holstein steers (197kg) with ruminal and proximal duodenal cannulas were used in a 4×4 Latin square design to compare effects of alfalfa vs sudangrass hay at 40 vs 80g/kg of forage aNDFom on digestion. There were no treatment effects on ruminal aNDFom digestion (P>0.10). Ruminal OM digestion was not affected by forage aNDFom level (P=0.96); however, it was greater (P=0.04) for sudangrass vs alfalfa supplemented diets. Microbial efficiency was not affected by forage aNDFom level (P=0.53); however, it was greater (P=0.02) for alfalfa vs sudangrass supplemented diets. Forage source did not affect total tract aNDFom digestion (P=0.86). However, aNDFom digestion was greater (P=0.08) for 80 vs 40g/kg of forage aNDFom. There were interactions between forage aNDFom level and source on total tract OM digestion (P=0.03). With alfalfa, level of forage aNDFom did not affect OM digestion. With sudangrass, increasing forage aNDFom decreased (P<0.05) total tract OM digestion. There were interactions between forage aNDFom level and source on ruminal pH (P=0.05), and acetate:propionate ratio. With alfalfa, increasing forage aNDFom did not affect ruminal pH, but increased acetate:propionate molar ratio. With sudangrass, the increasing forage aNDFom increased ruminal pH, but did not affect VFA molar ratios. Four Holstein steers (189kg) were used in a 4×4 Latin square design to compare effects of ground vs pelletized rice straw at 40 vs 80g/kg of forage aNDFom on digestion. There were no treatment effects (P>0.10) on ruminal OM digestion or microbial efficiency. Ruminal aNDFom digestion was greater (P=0.05) for pelletized vs ground rice straw. There were no treatment effects (P>0.10) on total tract aNDFom digestion. Pelletizing did not affect (P=0.78) total tract OM digestion. Tract OM digestion was greater (P=0.03) for 40 vs 80g/kg of forage aNDFom. Level of forage aNDFom did not affect (P>0.10) ruminal pH or VFA molar proportions. Ruminal pH and acetate:propionate molar ratio were greater (P<0.10) for ground vs pelletized rice straw. Differences among forage sources are minimized when forages are compared at similar levels of forage aNDFom concentration. Compared to ground, pelleted rice straw in finishing diets may produce lower ruminal pH and growth-performance of cattle.

Comparative feeding value of distillers dried grains plus solubles as a partial replacement for steam-flaked corn in diets for calf-fed Holstein steers: Characteristics of digestion, growth performance, and dietary energetics

Two experiments were conducted to examine the effect of level of dried distillers grains plus solubles (DDGS) supplementation (0, 10, 20, and 30%; DM basis), replacing steam-flaked (SF) corn in finishing diets, on characteristics of digestion (Exp. 1) and growth performance (Exp. 2) in calf-fed Holstein steers. In Exp.1, 4 cannulated Holstein steers (349 ± 12 kg) were used to evaluate treatment effects on characteristics of digestion. Ruminal NDF digestion tended to increase (quadratic effect, P = 0.09) and ruminal OM digestion decreased (linear effect, P = 0.01) with DDGS substitution. There were no treatment effects on duodenal flow of microbial N (MN). Substitution with DDGS increased (linear effect, P < 0.01) N flow to the small intestine. The undegradable intake protein (UIP) value of DDGS was 35%. Postruminal digestion of OM (linear effect, P = 0.04) and fatty acids (linear effect, P = 0.03) and total tract digestion of OM and GE decreased (linear effect, P < 0.03) with increasing level of DDGS substitution. Substitution with DDGS did not affect (P = 0.80) ruminal pH but increased (linear effect, P = 0.01) acetate:propionate molar ratio. In Exp.2, 144 Holsteins steer (112 ± 6 kg) were used in a 305-d trial to evaluate treatment effects on growth performance and carcass characteristics. During the initial 126 d, DDGS substitution increased ADG (linear effect, P = 0.03), G:F (quadratic effect, P = 0.03), and dietary NE (quadratic effect, P = 0.02), maximal for both at 20% DDGS inclusion rate. Based on estimated indispensable AA supply to the small intestine as a percentage of requirements during the initial 126-d period, histidine was first limiting followed by methionine. During the final 179-d period and overall (305-d feeding period), treatment effects on ADG and G:F were small (P ≥ 0.22). Compared with the other treatments, HCW was greater (3.4; P = 0.03) at the 20% level of DDGS substitution. The NE value for DDGS in SF corn-based diets for the calf-fed Holstein are consistent with current tabular standards. Extra-caloric value of DDGS as a metabolizable AA source is apparent during the initial growing phase. The UIP value of DDGS used in this study (35%) was considerably less than current tabular estimates (52%; NRC, 2000).

Effects of feeding different levels of chromium-enriched live yeast in hairy lambs fed a corn-based diet: effects on growth performance, dietary energetics, carcass traits and visceral organ mass

Forty Pelibuey × Kathdin lambs (35.5 ± 0.4 kg) were used in a 56-day feeding experiment to assess the effects of feeding different levels of chromium-enriched live yeast (Cr-YC) on growth performance, dietary energetics, carcass traits and visceral organ mass. The Cr-YC source contained 5.5 × 109 colony forming units (CFU) and 0.40 mg of Cr per gram. Treatments consisted of a dry rolled corn-based finishing diet supplemented with 0, 1, 2 or 3 g Cr-YC/lamb.day. Total daily dosages were: 5.5 × 109 CFU and 0.4 mg; 1.1 × 1010 CFU and 0.8 mg Cr, and 1.65 × 1010 CFU and 1.2 mg Cr for supplementation levels of 1, 2 or 3 g Cr-YC/lamb.day, respectively. There were no treatments effects on dry matter intake. As the level of Cr-YC supplementation increased, average daily gain, gain to feed and dietary net energy were linearly increased, and observed/expected dry matter intake was linearly decreased. Chromium-enriched live yeast supplementation increased empty bodyweight (EBW), gastrointestinal fill and full viscera weight, but did not influence organ weights as a proportion of EBW (g/kg EBW). Cr-YC level did not affect carcass length, backfat thickness, kidney, pelvic and heart fat or body wall thickness, but increased hot carcass weight and longissimus muscle area. In general, treatment effects on percentage yield of wholesale cuts (tissue weight as a percentage of cold carcass weight) were small. However, Cr-YC decreased percentage flank. Chromium-enriched yeast supplementation enhances growth rate, longissimus muscle area, and dietary energetic efficiency in finishing feedlot lambs.

Comparative effects of virginiamycin supplementation on characteristics of growth-performance, dietary energetics, and digestion of calf-fed Holstein steers

Two trials were conducted to evaluate the influence of virginiamycin supplementation on growth performance and digestive function. In trial 1, 144 Holstein steer calves (119 kg of initial BW) were used in a 340-d trial. Treatments were (DM basis) 1) control (no antibiotic); 2) 16 mg/kg of virginiamycin; 3) 22.5 mg/kg of virginiamycin; and 4) 28 mg/kg of monensin. Calves received a steam-flaked corn-based growing diet for the first 112 d and thereafter were fed a finishing diet from d 112 until slaughter. Steers were divided into 2 BW groups (lighter-half and heavier-half), and assigned within groups to 24 pens (6 steers/pen). Virginiamycin did not affect (P > 0.20) ADG, but increased 340-d G:F (linear; P = 0.02) and dietary NE (linear; P = 0.04). Improvements in dietary NE were most pronounced during the final 116 d of the feeding period (linear; P = 0.04) that comprised the hot summer months (June through September). Monensin did not affect (P > 0.20) growth performance or dietary NE. Although average initial BW of the lighter-half and heavier-half BW groups differed by only 4 kg, the heavier BW group had greater HCW (13 kg, P = 0.02), LM area (5.4%, P = 0.01), ADG (4.3%, P = 0.02), G:F (2.3%, P = 0.08), and dietary NE (3.2%. P = 0.01) than the lighter BW group. In trial 2, 4 Holstein steers (269 +/- 3.5 kg) with cannulas in the rumen and proximal duodenum were used in a 4 x 4 Latin square design to evaluate treatment effects on digestion. Virginiamycin did not affect (P > 0.20) ruminal digestion of OM, NDF, starch, and N, and microbial efficiency, but tended to linearly increase (P = 0.09) ruminal N efficiency (nonammonia N flow to the small intestine/N intake). Likewise, virginiamycin did not affect (P > 0.20) postruminal or total tract digestion of OM, starch, NDF, and N. Compared with the control diet, monensin depressed total tract digestion of OM (4.2%, P = 0.03) and NDF (29.5%, P = 0.02). There were no treatment effects (P > 0.20) on ruminal pH, or ruminal lactate and total VFA molar concentration. Virginiamycin increased ruminal molar proportion of acetate (quadratic; P = 0.04) and estimated methane production (quadratic; P = 0.07), and decreased propionate molar proportion (quadratic; P = 0.09). Monensin did not affect (P > 0.20) ruminal VFA molar proportions or estimated methane production. We concluded that virginiamycin supplementation can enhance feedlot growth-performance and dietary energetic efficiency of calf-fed Holstein steers.

Performance by feedlot steers and heifers: Daily gain, mature body weight, dry matter intake, and dietary energetics

Performance, DMI, diet composition, and slaughter data from 9,683 pens of steers and 5,009 pens of heifers that were fed high-concentrate diets for 90 d or more were obtained from 15 feedlots from the western United States and Canada. The data set included pen means for more than 3.1 million cattle fed between 1998 and 2004. Performance measurements assessed included ADG, DMI, dietary NE, shrunk initial weight (SIW), and shrunk final weight. Mature final weight (MFW) for cattle in each pen was estimated based on regression of slaughter weight against SIW and ADG across all pens. Equations were developed to standardize performance projections (ADG, MFW, and break-even values) and analyze feedlot cattle close-outs. Generally, as diet NE concentration increased, DMI was decreased but G:F, dressing percentage, and yield grade all increased. Pens of cattle with greater SIW had greater ADG, DMI, and shrunk final weight but a lower G:F and dressing percentage. Dressing percentage and yield grade were correlated positively. Equations of the NRC relating gain to NE intake explained 85 and 80% of the variation in DMI of steers and heifers, respectively, with mean ratios of predicted to observed DMI (DMIratio) at 1.000 +/- 0.0506 and 0.974 +/- 0.0490. However, a significant (P < 0.001) bias in the NRC estimate of DMI was detected (r(2) = 0.10 and 0.05, for steers and heifers) between the DMIratio and ADG in which DMIratio increased as ADG increased. This was due to inherent confounding of ADG and MFW in the original NE equation of Lofgreen and Garrett. Based on iterative optimization to minimize the difference between expected and observed DMI, revised equations for retained energy (RE, Mcal/kg) were developed for steers and for heifers: RE(steer) = 0.0606 x (LW x 478/MFW(steer))(0.75)ADG(0.905); RE(heifer) = 0.0618 x (LW x 478/MFW(heifer))(0.75)ADG(0.905), where LW = mean shrunk live weight. The revised equations decreased the SD of the DMIratio by 5.4% (from 0.0496 to 0.0469) and eliminated the bias in DMIratio that was related to ADG (r(2) = 0.0006). The similarity between the 2 equations derived for steers and for heifers for estimation of RE from ADG supports the concept that scaling by MFW accounts for energy utilization differences between sexes.

Dietary energetics of the insectivorous Mexican free-tailed bat (Tadarida brasiliensis) during pregnancy and lactation.

Stomach content analysis of 20 pregnant (x body mass=13.4 g) and 18 lactating (x body mass=11.5 g) female Tadarida brasiliensis revealed that the diet, expressed as percent volume, consists largely of lepidopterans, coleopterans, hymenopterans, and dipterans, in decreasing order of importance. We found no significant difference in the diet of pregnant and lactating females when expressed as percent volume. However, when expressed as percent frequency, proportionately more pregnant females fed on lepidopterans, coleopterans, and dipterans than did lactating bats, and proportionately more lactating females fed on hymenopterans. We found no significant differences in the percentages of water, lean dry mass, fat, and energy density in the stomach contents of pregnant and lactating females. Water in stomach contents averaged 62.7% and fat and lean dry mass averaged 22.2% and 15.2%, respectively (expressed as percentage of wet mass); energy density averaged 31.2 kJ g-1 dry mass. This relatively high energy density of stomach contents, as compared to whole insects, can be attributed to the consumption of insects high in fat (especially flying ants) and the abdomens only of moths and beetles (other body parts being discarded). Estimates of nightly food intake increased markedly from mid- to late pregnancy, stabilized or decreased during late pregnancy, and increased again during early to mid-lactation. Average nightly feeding rate doubled from pregnancy to lactation and increased threefold during the first half of lactation. By adjusting our gravimetric estimates of nightly feeding rate upwards by 40% (based on estimates of field metabolic rate), we estimate that the average nightly energy intake of female T. brasiliensis ranges from 57 kJ day-1 in early lactation to 104 kJ day-1 in mid-lactation. These estimates represent nightly feeding rates averaging 39% and 73% of a female's body mass in the period from early to mid-lactation, respectively.

Ontogenetic Changes in Diet, Field Metabolic Rate, and Water Flux in the Herbivorous Lizard Dipsosaurus dorsalis

Dietary energetics of Dipsosaurus dorsalis were studied to test the hypothesis that high mass-specific food energy requirements of small (3.5 g) hatchlings preclude herbivory in early life. This was not the case. Hatchlings were no less herbivorous than adults; arthropod prey constituted only 1% of the diet of both age classes. Field metabolic rate, water turnover, and feeding rate were measured in different seasons for separate age classes using doubly labeled water $(H^{3}H^{18}O)$, and assimilation efficiency was measured for different age classes eating natural and exclusively arthropod (Tenebrio larvae) diets. Compared to spring vegetation, Tenebrio diets yielded 2.3 times the assimilable energy per g dry matter consumed, owing to higher energy content and greater assimilability of the arthropods. Assimilation efficiency for the natural autumn diet during a drought season declined to 0.29 in adult Dipsosaurus; however, hatchling lizards maintained an assimilation efficiency of 0.54 by specializing on...