Dietary Canola Research Articles

494 Long-term effects of canola meal inclusion in gestation and lactation diets on sow and piglet performance over two reproductive cycles

Abstract The experiment was conducted to determine the long-term effects of dietary canola meal (CM) inclusion as the main protein source in gestation and lactation diets on sow and litter performance. Sows (n = 68) from two baches were randomly allotted 1 of 2 diets on d 80 of pregnancy. The two diets were a corn-barley and soybean meal control diet (CTRL) and a corn-barley and CM treatment diet (CCM). All diets were formulated to be similar in standardized ileal digestible amino acid and net energy contents. Sow body weight (BW) and backfat (BF) thickness were determined on d 1, 35, 80, and 111 of pregnancy, on d 1 and 21 post-farrowing. Piglets were weighed on d 0 and at weaning (d 21). Data were analyzed using the PROC MIXED procedure of SAS 9.4 for a randomized complete block design with repeated measures. Over the two reproductive cycles, dietary treatments did not influence sow BW and BF thickness on d 80 and 111 of pregnancy, as well as d 1 and 21 post-farrowing (P > 0.10). However, sows fed the CCM diet had less (P < 0.05) BW and BF thickness on d 1 and 35 of pregnancy in the second reproductive cycle than sows fed the CTRL diet. The CCM diet decreased gestation gain from d 80 to d 111 of pregnancy compared with the CTRL diet over the two reproductive cycles (P < 0.05). No dietary effect on litter size or litter performance was found during the experimental period (P > 0.10). However, reproductive cycle had significant effects (P < 0.05) on BW gain and BF thickness from d 80 to 111 of pregnancy as well as BW and BF thickness on d 1 of lactation. In addition, litter weight at birth and after cross-fostering were significantly improved during the second reproductive cycle compared with the first cycle (P < 0.05). An interaction between dietary treatment and reproductive cycle on BW gain and BW on d 1 of lactation was observed (P < 0.05). In conclusion, feeding gestation and lactation diets containing CM as the main protein source to sows over two reproductive cycles supported similar sow reproductive and litter performance as the control.

PSIV-A-10 Effects of Canola Meal Inclusion in Gestation and Lactation Diets with or without Probiotic on sow Performance, Milk Composition and Piglet Performance

Abstract The experiment was conducted to determine the effects of high dietary canola meal (CM) inclusion in gestation and lactation diets with or without probiotic supplementation on sow and litter performance and milk composition. Seventy-five sows were randomly allotted 1 of 3 diets to give 25 replicates per treatment. Diets consisted of a corn and soybean meal (SBM) control (CTRL) or the CTRL diet with SBM replaced by 300g/kg CM and fed with (CM+) or without (CM-) probiotic ActisafÒ Sc 47 supplementation. Sows BW and backfat thickness were determined on d 80 and 111 of gestation, d 1 post-farrowing, and at weaning on d 21. Milk samples were collected on d 1 and d 21 post-farrowing to determine milk composition. Piglets were weighed on d 0 and at weaning. Data were analyzed using the PROC MIXED procedure of SAS 9.4 for a randomized complete block design. Dietary treatment had no effect on sow performance, milk crude protein and lactose oligosaccharide composition, piglet ADG and weaning weight, and piglet survivability (P > 0.10). Sows fed CM- diet tended to fewer (P = 0.07) weaned piglets compared to sows fed CTRL diet, but no difference was found between sows fed the CM+ and CTRL diets (P > 0.10). At weaning, milk fat content for sows fed with CM+ diet was higher (P < 0.05) and tended to be higher (P = 0.09) compared with those fed the CTRL and the CM- diet, respectively. In conclusion, including 300g/kg CM in gestation and lactation sow diets supported similar sow and piglet performance as those the control diet, without affecting piglet survivability at weaning. Also, probiotic supplementation in CM diet increased sow milk fat content at weaning.

Effects of selected dietary plant oils on survival, growth, fatty acid profile, and enzyme activity of a tropical hybrid carp (Barbonymus gonionotus ♀ × Hypsibarbus wetmorei ♂) larvae

Hybrid lemon fin barb is a new tropical carp species developed by crossing silver barb (Barbonymus gonionotus, ♀) with lemon fin barb (Hypsibarbus wetmorei, ♂) to cover the production gap in Malaysian aquaculture, as the tasty wild H. wetmorei is being overexploited. Different plant oils such as crude palm oil (CPO), linseed oil (LO), canola oil, sunflower seed oil and soybean oil were tested for the larval stage of this fast-growing and delicious carp species. Three-day old hybrid larvae were arbitrarily assigned to twenty 5-L tanks with a rate of 50 larvae L−1. After 20 days of the feeding trial, weight gain, total length gain and specific growth rate (SGR) of hybrid larvae fed diets containing CPO and LO were significantly higher (p < 0.05) than those of larvae fed with soybean oil. Survival of larvae fed CPO was significantly higher (p < 0.05) than those of larvae fed LO, canola oil and soybean oil diets. Total saturated fatty acids (SFAs) reported were high in hybrid larvae fed soybean oil followed by CPO while larvae fed CPO had the highest (p < 0.05) total body monounsaturated fatty acids (MUFAs). Total polyunsaturated fatty acids (PUFAs) of larvae fed CPO, LO, sunflower seed oil and soybean oil were significantly reduced (p < 0.05) accordingly. The larvae fed the CPO diet had the highest (p < 0.05) docosahexaenoic acid (DHA), arachidonic acid (ARA), total n-3 fatty acids and n-3/ n-6 ratio while dietary CPO and linseed oil increased larval body eicosapentaenoic acid (EPA) content. There was a significant interaction (p < 0.05) in dietary linoleic acid (LA) and ARA provided by different plant oils on ARA in hybrid larval body. Moreover, among plant oils used in the experiment, CPO was fetched at the lowest price. In conclusion, the use of dietary CPO in hybrid lemon fin barb larval diets is more economical and feasible.

Effects of dietary various oil sources on zootechnical performance, sexual maturation, whole‐body proximate composition and fatty acid profile of Hediste diversicolor

The ragworm, Hediste diversicolor, is one of the few polychaeta species to present a commercial interest in Iran. In this study, the effects of six oil sources in the diet, namely fish oil (FO), soyabean oil (SBO), canola oil (CAO), corn oil (COO), sunflower oil (SFO) and mixed vegetable oil (MO), on growth, survival, fatty acid profile and biometric measurements of H. diversicolor were conducted during eight weeks. Furthermore, the mean survival rate of H. diversicolor reared on MO (82.93 ± 2.01%) diet, vice versa SGR, WG, Final biomass and PER, was significantly (p < 0.05) lower than that H. diversicolor fed the FO (89.40 ± 3.00%) diet. These findings suggested that MO could supply sufficient essential fatty acids, especially n3 and n6 and effective on zootechnical performance, survival and gonad maturation for juvenile H. diversicolor and should be given to individuals considered for production of dry feeds (and cheaper) of H. diversicolor. The results revealed a higher proportion of n3 HUFA in whole body of H. diversicolor than those in the experimental diets and values of n-3/n-6 in H. diversicolor fed the vegetable diets ranged from 0.13 in the COO diet to 0.21 in the CAO diet, meanwhile the values of n-3/n-6 in H. diversicolor fed the vegetable diets were lower than that obtained for the MO treatment. The FA such as C16:0, C17:0, C18:0 and C20:4ω-6 had a higher concentration in experiment groups than in control. Vegetable oils, especially mix vegetable oils, were economic alternatives to the expensive fish oil used in the H. diversicolor aquaculture industry.

Differential retention of duplicated retinoid-binding protein (crabp &rbp) genes in the rainbow trout genome after two whole genome duplications and their responses to dietary canola oil

The complexity of retinoid intracellular signaling by lipid-binding proteins is further confounded in salmonids, owing to a whole genome duplication (WGD), followed by a second salmonid-specific WGD. As such, many more cellular retinoic acid-binding protein (crabp)/retinol-binding protein (rbp) genes may exist in salmonids, compared to other vertebrate species. In this study, genomic database searches and phylogenetic analyses identified seven crabp and eight rbp genes in the rainbow trout. A parsimonious explanation for the number of crabp/rbp genes in the rainbow trout genome is duplicated genes were lost from the genome by nonfunctionalization following the salmonid-WGD, and the extant duplicate crabp/rbp genes of the much earlier teleost-WGD have been retained by either sub- or neofunctionalization of tissue-specific regulatory mechanisms. Nutritional regulation of retinoid-binding protein genes in the liver was also determined in the fish fed diets containing various amounts of canola oil (CO) for 12 weeks along with hepatic fatty acid profile, Vitamin A (VitA) level, and growth parameters. The crabp/rbp transcripts were modulated markedly by experimental diets except for rbp2a.1/rbp2a.2 at the end of the trial. This result suggests that rbp2a.1/rbp2a.2 don't exhibit obvious divergence of transcriptional regulatory mechanisms that control their nutritional regulation by dietary CO. Growth parameters didn't differ markedly among dietary treatments. An understanding of VitA homeostasis, especially the regulatory effects of retinoids on transcriptional control of target genes, may have considerable importance to basic fish development, physiology, and aquaculture and this study may lead to future research on molecular VitA metabolism especially in salmonids experienced salmonid-WGD.

Effects of different dietary canola and fish oil levels on overall performance, fatty acid profile, haemato‐biochemical responses, and digestibility of macronutrients of Caspian brown trout ( Salmo trutta caspius Kessler) fingerling

A 2-month feeding trial was undertaken to evaluate the effects of replacing dietary fish oil (FO) with canola oil (CO) at four levels of HF (100% fish oil), FF (70% fish oil + 30% canola oil), FV (50% fish oil + 50% canola oil), and HV (100% canola oil) on overall performance, haemato-biochemical responses, fatty acid composition and digestibility of Caspian brown trout, Salmo trutta caspius (Kessler) fingerlings. Fingerlings (N = 180, 4.6 ± 0.23 g) were randomly scattered to four various diet treatments. In fingerlings fed with FV diet a considerable increase in specific growth rate and weight gain were recorded (p < .05). No significant difference was observed in none of the macronutrients of the whole-body among the treatments (p > .05). The highest saturated fatty acids and the lowest monounsaturated fatty acids were recognized in the HF diet (p < .05). The lowest red blood cell was found in fingerlings fed HF diet (p < .05), but the white blood cell activity was not influenced in the groups (p > .05). The fingerlings that consumed the HF diet implied the highest aspartate transaminase, alanine transaminase, alkaline phosphatase and lactate dehydrogenase activities in serum than the other groups (p < .05). None of the whole-body compositions such as protein, lipid, ash, and moisture were affected by dietary treatments (p > .05). The lowest value of apparent digestibility of crude lipid (ADCCL) was observed in the HF group (p > .05). Overall, the data exhibited that elevating dietary canola oil up to 50% in Caspian brown trout fingerling resulted to improve growth efficiency and blood biochemistry.

266 Growth Performance, Fecal Score, and Blood Immune Parameters of Nursery Pigs Challenged with Escherichia coli F18 Fed Canola Meal-based Diet

Abstract Effects of dietary canola meal (CM) on growth performance, fecal score, and blood parameters of E. coli-challenged nursery pigs were investigated. Thirty-six pigs (initial body weight = 6.22 kg) weaned at 21 d of age were housed individually and fed 3 diets (12 replicates/diet) in randomized complete block design. The diets were corn-soybean meal-based diet without or with antibiotics (0.2% chlortetracycline and 0.2% tiamulin) or with CM (20%). The diets were fed in 2 phases; Phase 1: d 0 to 7, and Phase 2: d 7 to 21. Pigs were orally challenged with F18 strain of E. coli on d 7. Feed intake and body weight were measured on d 7, 14, and 21. Blood samples for measuring thyroid hormones were collected from each pig on d 7, 14, and 21. Blood samples for measuring white blood cells, immunoglobulins, and cytokine were collected on d 21. Fecal score was assessed daily. Dietary antibiotics increased (P &amp;lt; 0.05) ADG by 50%. Dietary SECM also increased (P &amp;lt; 0.05) ADG by 23%. Week 1 fecal score was unaffected by diet. However, during weeks 2 and 3, fecal score for diet with antibiotics was less (P &amp;lt; 0.05) than that for basal diet or diet with CM, which did not differ in fecal score. During weeks 1 and 2, serum tetraiodothyronine level for diet with antibiotics was less (P &amp;lt; 0.05) than that for basal diet or diet with CM, which did not differ in serum tetraiodothyronine level; week 3 serum tetraiodothyronine level was unaffected by diet. White blood cell count was reduced (P &amp;lt; 0.05) by dietary antibiotics, and tended to be reduced (P = 0.087) by dietary CM. In conclusion, dietary CM increased ADG and tended to reduce white blood cell count. Thus, dietary CM at 20% may improve the performance of pigs challenged with E. coli F18 partly by decreasing immune response.

Effects of dietary different lipid sources on plasma metabolites in silvery-black porgy (Sparidentex hasta) juveniles

In the current study the plasma metabolites of silvery-black porgy (Sparidentex hasta) juveniles fed different lipid sources were analyzed in order to bio-monitoring the fish health condition. In this regard, an eight-week feeding trial was conducted using seven isonitrogenous (50%) and isolipidic (20%) experimental diets namely: FO, CO (canola oil), SO (sunflower oil), T (tallow), FC (fish oil + canola oil, 50:50), FS (fish oil + sunflower oil, 50:50) and FT (fish oil + tallow, 50:50). Fish fed with SO diet had the highest liver lipid content. Liver n−9 monounsaturated fatty acids, n−6 and n−3 poly unsaturated fatty acids were highest in fish fed the CO, SO and FO, respectively (P < 0.05). Fish fed FO and T diets had the highest plasma total cholesterol, whereas fish fed CO and SO diets had the highest plasma triglyceride and VLDL levels (P < 0.05). Plasma ALP and ALT were highest in fish fed SO and FO groups, respectively. Fish fed FS diet had the highest plasma TBARS concentration, whereas plasma CAT activity was highest in fish fed FO and FS diets. The results of this study demonstrated that different dietary lipid sources can drastically change fatty acid profile of the liver which consequently can alter different metabolic pathways that can affect plasma metabolite health indices in silvery-black porgy juveniles.

Dietary soybean oil, canola oil and partially-hydrogenated soybean oil affect testicular tissue and steroid hormone levels differently in the miniature pig

The present study was conducted to examine the influence of dietary canola oil (CAN) and partially-hydrogenated soybean oil (HSO) compared to soybean oil (SOY, control) on the morphology and function of testes using miniature pigs as the test subject. Male miniature pigs were fed a diet containing 10%SOY, 9%CAN+1%SOY, or 9%HSO+1%SOY for 18 months. The scheduled autopsies revealed no abnormalities in histopathological examination of the major organs, except the testes. Atrophy of the seminiferous tubules and hyperplasia in the Leydig cells were found in the SOY and CAN groups. DNA microarray analysis indicated downregulation in the CAN and the HSO groups of genes encoding for gonadotropins in the pituitary gland and of enzymes and proteins involved in steroid hormone metabolism in the testes, compared to the SOY group. Plasma levels of sex hormones in the CAN and HSO groups tended to be higher and testosterone and dihydrotestosteorne in the HSO group were significantly higher than in the SOY group. These results demonstrate that testes are morphologically and functionally affected by the dietary oils, while the plasma steroid hormone levels do not necessarily reflect the gene expression, probably owing to feedback regulation via the gonadal hormones in the hypothalamus-pituitary-gonadal axis.

Abstract P293: Effects of Diets That Vary in Fatty Acid Composition on Fecal Short-chain Fatty Acid Levels and Their Relationship With Circulating Lipids and Lipoproteins

Introduction: The short-chain fatty acids (SCFA) acetic acid, propionic acid, and butyric acid are microbial-produced metabolites that can influence host physiology through regulation of hepatic cholesterol metabolism. These biologically relevant gut metabolites may play a role in the hypocholesterolemic effects of select dietary components. The objective of this exploratory study was to determine the effects of diets that differ only in fatty acid composition on fecal SCFA levels and to assess their correlations with circulating lipids, lipoproteins, and apolipoproteins. Hypothesis: We assessed the hypothesis that dietary fat quality will differentially affect fecal SCFA and there will be significant associations between fecal SCFA levels and those of circulating total cholesterol, low-density lipoprotein-cholesterol (LDL-C), non-high-density lipoprotein-cholesterol (non-HDL-C), and apolipoprotein (apo) B. Methods: In a double-blind, randomized, three period crossover, controlled feeding clinical trial, participants with ≥2 metabolic syndrome criteria (n=20) were provided with a weight maintenance, controlled feeding base diet plus conventional canola oil, high-oleic acid canola oil (HOCO), or a control oil (control diet formulated to represent a Western diet fatty acid profile) for 6 weeks followed by washout periods of ≥4 weeks. The macronutrient profiles of the diets were: canola diet [17.5% monounsaturated fatty acid (MUFA), 9.2% polyunsaturated fatty acid (PUFA), 6.6% saturated fatty acid (SFA)], HOCO diet (19.1% MUFA, 7.0% PUFA, 6.4% SFA), and control diet (10.5% MUFA, 10.0% PUFA, 12.3% SFA). Fecal and blood samples were collected at study enrollment and at the end of each diet. Results: After 6 weeks, a trend toward a treatment effect on endpoint fecal propionic acid was observed ( P =0.09), with a trend toward a higher concentration following the control compared to the canola diet ( P =0.09). Acetic acid was increased from baseline following the control diet ( P =0.04). After the control diet only, fecal levels of propionic acid were positively correlated with blood levels of LDL-C, non-HDL-C, and apo B (r=0.52 to 0.64, P =0.003 to 0.02), with a trend for total cholesterol (r=0.39, P =0.10), and acetic acid was positively correlated with LDL-C and apo B levels (r=0.48 to 0.49, P =0.03 to 0.04), with a trend for non-HDL-C (r=0.44, P =0.06). No significant correlations between fecal SCFA and lipids and lipoproteins were observed after the two canola oil-based diets. Conclusions: In conclusion, these data suggest that the adverse effects of a contemporary Western diet fatty acid profile on circulating lipid and lipoprotein parameters compared to diets higher in unsaturated fat and lower in SFA may be mediated by gut-derived SCFA.

Diets Enriched with Conventional or High-Oleic Acid Canola Oils Lower Atherogenic Lipids and Lipoproteins Compared to a Diet with a Western Fatty Acid Profile in Adults with Central Adiposity

Novel oils high in monounsaturated fatty acids (MUFAs) and low in saturated fatty acids (SFAs) are an alternative to partially hydrogenated oils high in trans-unsaturated fatty acids. There is widespread use of high-MUFA oils across the food industry; however, limited knowledge of their cardiovascular impact exists. We investigated the effects of diets containing canola oil, high-oleic acid canola oil (HOCO), and a control oil blend (diet formulated to emulate a Western fat profile) on lipids, lipoproteins, and apolipoproteins (apos), as secondary outcomes of the trial. In a multi-center, double-blind, randomized, 3-period crossover, controlled feeding trial, men (n=44) and women (n=75) with a mean age of 44 y, mean body mass index (BMI; in kg/m2) of 31.7, and an increased waist circumference plus≥1 metabolic syndrome criteria consumed prepared, weight-maintenance diets containing canola oil [17.5% MUFAs, 9.2% polyunsaturated fatty acids (PUFAs), 6.6% SFAs], HOCO (19.1% MUFAs, 7.0% PUFAs, 6.4% SFAs), or control oil (10.5% MUFAs, 10.0% PUFAs, 12.3% SFAs) for 6 wk with ≥4-wk washouts. Fasting serum lipids were assessed at baseline and 6 wk. Diet effects were examined using a repeated measures mixed model. Compared with the control, canola and HOCO diets resulted in lower endpoint total cholesterol (TC; -4.2% and -3.4%; P<0.0001), LDL cholesterol (-6.6% and -5.6%; P<0.0001), apoB (-3.7% and -3.4%; P=0.002), and non-HDL cholesterol (-4.5% and -4.0%; P=0.001), with no differences between canola diets. The TC:HDL cholesterol and apoB:apoA1 ratios were lower after the HOCO diet than after the control diet (-3.7% and -3.4%, respectively). There were no diet effects on triglyceride, HDL cholesterol, or apoA1 concentrations. HOCO, with increased MUFAs at the expense of decreased PUFAs, elicited beneficial effects on lipids and lipoproteins comparable to conventional canola oil and consistent with reduced cardiovascular disease risk in adults with central adiposity. This trial was registered at www.clinicaltrials.gov as NCT02029833.

180 Effect of High Dietary Canola Meal Inclusion in Gestation and Lactation Sow Diets with or without Enzyme Supplementation on Reproductive Performance, Milk Composition and Nutrient Digestibility.

180 Effect of High Dietary Canola Meal Inclusion in Gestation and Lactation Sow Diets with or without Enzyme Supplementation on Reproductive Performance, Milk Composition and Nutrient Digestibility.

Abstract P382: Canola and High-Oleic Acid Canola Oils Improve Lipid/Lipoprotein Parameters Compared to an Oil Blend Characteristic of a Western Dietary Pattern in Individuals at Risk for Metabolic Syndrome: A Randomized Crossover Clinical Trial

Introduction: Identifying dietary interventions for cardiometabolic disease prevention in individuals with metabolic syndrome is relevant to a significant portion of the population. Numerous studies have investigated the effects of canola oil on cardiovascular disease risk; however, no studies have compared canola oil diets to a control diet with a fatty acid composition characteristic of Western intakes in individuals with metabolic syndrome risk factors. The objectives of this study were to evaluate effects of canola oil, high-oleic acid canola oil (HOCO), and a control oil (blend of butter, safflower, coconut, and flaxseed oils formulated to represent a Western diet fatty acid profile) on lipids, lipoproteins, and apolipoproteins. Hypothesis: We tested the hypothesis that the two canola oil diets would elicit beneficial effects on the total lipid/lipoprotein profile compared to the Western (control oil) diet. Methods: In a multi-center, double blind, randomized, three-period crossover, controlled feeding clinical trial, 119 individuals with an increased waist circumference plus at least one additional metabolic syndrome risk factor consumed prepared isocaloric, weight maintenance diets containing canola oil [17.5% E from monounsaturated fatty acids (MUFA), 9.2% polyunsaturated fatty acids (PUFA), 6.6% saturated fatty acids (SFA)], HOCO (19.1% E from MUFA, 7.0% PUFA, 6.4% SFA), or control oil (11% E from MUFA, 10% PUFA, 12% SFA) for six-weeks each separated by 4-12 week washouts. The differences at the end of 42 days of feeding were tested. Results: The canola oil and HOCO resulted in lower endpoint total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), the TC: high-density lipoprotein-cholesterol (HDL-C) ratio, apolipoprotein (apo) B, the apoB: apoA1 ratio, and non-HDL-C compared to control oil ( P &lt;0.0001 for treatment effect), with no differences between HOCO and canola oil for these parameters. Endpoint apoA1 did not significantly differ between the two canola oils and control, but was higher after the HOCO compared to canola oil (1.46 ± 0.02 g/L vs. 1.43 ± 0.02 g/L, P = 0.0462). There were no differences among the three diets in endpoint triglycerides or HDL-C. Conclusions: Incorporating canola or high-oleic acid canola oils into the diet improves blood lipids and lipoproteins compared to a contemporary Western diet in individuals with at least two criteria for metabolic syndrome.

Use of sweet lupin, canola oilcake and full-fat canola as alternatives to soybean oilcake in diets for broilers

CITATION: Brand, T. S., et al. 2018. Use of sweet lupin, canola oilcake and full-fat canola as alternatives to soybean oilcake in diets for broilers. South African Journal of Animal Science, 48(3):553-562, doi:10.4314/sajas.v48i3.16.

Impacts of increasing levels of canola meal in diets of high producing Holstein cows on their productive performance

Ingredient substitution studies are common in the animal science literature. However such studies often confound changes of ingredient and nutrient levels, thereby begging the question as to whether responses measured among diets represent known differences among diets in measured nutrient levels, or to changes in unknown dietary factors/nutrients. Our objective was to determine if formulating diets to contain the same levels of nutrients, concomitant with increasing the dietary canola meal (CM) level from 50 to 170 g/kg of diet dry matter (DM), while mainly reducing levels of distillers dried grains (DDG), would result in dissimilar performance by lactating dairy cattle – thereby suggesting nutritional factors/nutrients of CM and/or DDG exist which are not known and so not included in diet formulation. This differs from Swanepoel et al. (2014; Anim. Feed Sci. Technol. 189, 41–53) wherein increasing levels of CM were confounded with modest changes in dietary levels of some proximate nutrients. The experiment was a 4 × 4 Latin square using 4 pens of about 315 early lactation multiparous cows/pen with four 21 day periods. The DM intake was not affected (30.0 ± 0.09 kg/d) by increased dietary levels of CM, but milk, milk lactose and milk energy outputs all declined (P < 0.01) linearly, as did milk somatic cell counts. Change in body condition score (BCS) declined linearly (P < 0.01), with a quadratic component (P < 0.01), suggesting maximum BCS gain at about 80 g/kg CM inclusion. Plasma concentrations of most essential amino acids increased linearly (P < 0.01) with higher dietary levels of CM – exceptions being Met and Phe (unaffected) and Leu (declined). As a result of lower milk energy output and lower accretion of energy in BCS gain, as well as similar DM intake, estimated net energy for lactation (NEl) of the diet declined linearly as inclusion of CM increased. While our objective of creating diets with no changes in dietary nutrient levels and biological measures was mainly met, there was an unexpected linear (P < 0.01) decrease of 6.3 g/kg DM in the crude fat level of the diets concomitant with the increase in CM inclusion. However the calculated decrease of diet NEl as a direct result of this difference in diet crude fat only accounted for about 40% of the decline in the estimated NEl density of the diet (i.e., 0.08 MJ/kg DM of NElversus the actual decline of 0.21 MJ/kg DM of NEl). This suggests that if the diets had achieved equal fat levels, as well as other nutrients, concomitant with increasing levels of CM, that NEl output of the cows would have still declined. Thus diets formulated with 170 g/kg of CM in diet DM (from 50 g/kg DM of CM) in substitution for primarily corn based DDG, but with equal levels of primary nutrients, resulted in a small reduction in performance of lactating dairy cows for reasons which are unknown, but may relate to lower conversion of digestible to metabolizable energy of the soluble carbohydrate fraction of CM versus DDG.

Evaluating the economics of short-term partial mixed ration feeding decisions for dairy cows

Recent research in Australia has measured the marginal milk production responses of cows grazing perennial-based pastures to supplements offered as a partial mixed ration (PMR). In the present study, the milk responses to PMR feeding developed under a restricted pasture allowance were used to examine the contribution to farm profit of decisions about short-term feeding (weekly, monthly). A short-term, or tactical, decision was considered to be one where the infrastructure and equipment needed to mix and feed out a formulated ration were already available. The decision was, therefore, the choice of whether to feed supplements as grain in the dairy at milking and forage in the paddock, or to feed the supplements as a mixed ration on a feed pad. Both of these options were assumed to exist for the farmer decision-maker, with the comparison of rations being based solely on the costs of supplement and milk income. The Control diet in the experiments comprised cereal grain fed in the dairy and pasture silage fed in the paddock, simulating a situation where pasture available for grazing was limiting. The PMR diet consisted of cereal grain, maize grain, maize silage and lucerne hay combined in a mixer wagon and fed on a feed pad. The PMR + canola diet was similar to the PMR diet, but included canola meal. The profitability of the diets for different amounts of supplement intake was compared by estimating the total income from milk produced minus the cost of the supplements. The results indicated that feeding the diet comprising PMR plus canola meal in early lactation contributed more to farm profit than did the Control diet of feeding grain in the dairy and forage in the paddock, or PMR feeding without canola meal, because of higher milk production. At a supplement intake of 12 kg DM/cow.day, the PMR + canola diet added AU$0.97/cow.day and AU$2.11/cow.day more to profit than the Control and PMR without canola meal diets, respectively. For a farm already equipped with a feed pad and mixer wagon, the benefits of feeding a mixed ration exceed the costs in early lactation. In late lactation, each diet made similar contributions to farm profit because of similar milk production.

Effects of replacing soybean meal with canola meal or treated canola meal on ruminal digestion, omasal nutrient flow, and performance in lactating dairy cows

Extrusion treated canola meal (TCM) was produced in an attempt to increase the rumen-undegraded protein fraction of canola meal (CM). The objective of this study was to evaluate the effects of replacing soybean meal (SBM) with CM or TCM on ruminal digestion, omasal nutrient flow, and performance in lactating dairy cows. To assess performance, 30 multiparous Holstein cows averaging (mean ± SD) 119 ± 23 d in milk and 44 ± 7 kg of milk/d and 15 primiparous cows averaging 121 ± 19 d in milk and 34 ± 6 kg of milk/d were blocked in a randomized complete block design with a 2-wk covariate period and 12-wk experimental period (experiment 1). Dietary ingredients differed only in protein supplements, which were SBM, CM, or TCM. All diets were formulated to contain (dry matter basis) 30% alfalfa silage, 30% corn silage, 4% soy hulls, 2.4% mineral-vitamin premix, and 16% CP. The SBM diet contained 25% high-moisture shelled corn and 8.6% SBM; the canola diets contained 22% high-moisture shelled corn and either 11.2% CM or 11.4% TCM. To assess ruminal digestion and omasal nutrient flow, 6 rumen-cannulated cows were blocked into 2 squares of 3 cows and randomly assigned within blocks to the same 3 dietary treatments as in experiment 1 in a replicated 3 × 3 Latin square design (experiment 2). Data were analyzed using the MIXED procedure of SAS (SAS Institute, Cary, NC). Orthogonal contrasts were used to compare effects of different protein supplements: SBM versus CM + TCM and CM versus TCM. In experiment 1, compared with SBM, apparent total-tract digestibilities of dry matter and nutrients were greater in cows fed both CM diets, and there was a tendency for nutrient digestibilities to be higher in cows fed CM compared with TCM. Diets did not affect milk yield and milk components; however, both canola diets decreased urinary urea N (% of total urinary N), fecal N (% of total N intake), and milk urea N concentration. In experiment 2, compared with SBM, both canola diets increased N intake and tended to increase rumen-degraded protein supply (kg/d) and N truly digested in the rumen (kg/d). Diets did not affect ruminal digestibility, efficiency of microbial protein synthesis, and rumen-undegraded protein flow among diets. Results from this experiment indicate that replacing SBM with CM or TCM in diets of lactating cows improved digestibility and may reduce environmental impact. Moreover, under the conditions of the present study, treating CM by extrusion did not improve CM utilization.

Effect of increasing dietary canola meal inclusion on lactation performance, milk composition, and nutrient digestibility of lactating sows.

The aim was to determine the effects of increasing dietary canola meal in substitution for soybean meal in lactation sow diets. Forty-five sows with an average parity of 1.8 (SD = 0.83) were randomly assigned to 1 of 3 dietary treatments ( = 15) consisting of a corn-based control diet and 2 diets with 15% and 30% canola meal formulated by replacing soybean meal with canola meal. Diets were formulated to be similar in standardized ileal digestible AA content and NE value and to meet or exceed nutrient recommendations for lactating sows. Sows were moved to farrowing on d 111 of gestation and fed the experimental diets until weaning on d 21. Sows were fed 3.0 kg/d from d 111 of gestation until parturition. After farrowing, feed was gradually increased through d 6 after which the diets were offered on an ad libitum basis until weaning. Sows were weighed and backfat thickness measured on d 111 of gestation and also on d 0, 7, and 21 postfarrowing. Litters were weighed on d 0, 7, and 21. Weaning to estrus interval in sows was also recorded. Blood and milk samples were collected 2 h postfeeding from sows on d 0, 7, and 21 and analyzed for plasma urea nitrogen (PUN) and milk composition. Fecal samples were collected on d 10, 11, and 12 postfarrowing to determine energy and nutrient digestibility. There were no dietary effects on lactation feed intake, sow BW and backfat change, weaning to estrus interval, and milk fat, protein, lactose, and urea composition. Also, there were no dietary effects on piglet ADG ( 0.10). Sows fed diets containing 15% and 30% canola meal had lower (linear, < 0.05) PUN values compared with those fed the control diet on d 0, 7, and 21 postfarrowing. Apparent total tract digestibility of DM, GE, CP, and P were reduced linearly ( < 0.05) with increasing canola meal inclusion. In conclusion, up to 30% canola meal in lactation diet can support satisfactory sow and suckling piglet performance.

&lt;b&gt;Productive performance, egg quality and bone characteristics of quails fed with meal and canola oil

This study evaluated the effect of canola meal and oil in quail diet on productive performance, egg quality and bone characteristics. Quails (n = 84) with 150-days-of-age and average weight of 234±17 g were used in this experiment that lasted 84 days. The experiment consisted of a completely randomized design with 4 treatments, 7 replications with 3 birds each. Four diets were formulated: SM+SO - control diet with soybean meal and oil; MC+SO - 25% replacement of soybean meal with canola meal; MC+CO - 25% replacement of soybean meal with canola meal and complete replacement of canola oil with soybean oil; SM+OC - control diet with soybean meal and canola oil. Productive performance, internal and external egg quality and bone characteristics were evaluated. The diet with canola meal and oil (MC+CO) decreased (p &lt;0.05) feed conversion. Albumen height was lower (p &lt; 0.05) for treatments with canola meal and oil (MC+CO). Regardless of the oil used, the dietary canola meal (MC+SO and MC+CO) reduced the weight and length of tibia (p &lt; 0.05), but increased its ash content (p &lt; 0.05). The mixture of canola oil and meal decreases feed conversion, and negatively affects internal egg quality and bone characteristics of quails.

Influence of Canola Oil and Lysine Supplementation Diets on Growth Performance and Fatty Acid Composition of Meat in Broiler Chicks

Influence of Canola Oil and Lysine Supplementation Diets on Growth Performance and Fatty Acid Composition of Meat in Broiler Chicks