Bioactive Dietary Components Research Articles

Flaxseed Bioactive Compounds and Colorectal Cancer Prevention.

Flaxseed and its bioactive components have been associated with a decreased risk of colorectal cancer incidence and progression. This review aims to summarize recent research regarding the role of flaxseed and each of its major dietary bioactive components in reducing colorectal cancer. In both human and animal model experiments, flaxseed consumption had beneficial effects on colon physiology associated with reduction in colorectal cancer risk or occurrence. Considered separately, each of flaxseed's major bioactive components, including fiber, alpha-linolenic acid, lignans, and other phytochemicals, is also associated with decreased risk of colonic neoplasms and regulation of cell growth through several potential mechanisms. Collectively, experimental data suggests that consumption of flaxseed and/or its bioactive components may reduce colorectal cancer risk by a variety of mechanisms. Future studies should focus on the mechanisms by which whole flaxseed can prevent colorectal cancer.

Epigenetics in Multiple Sclerosis: Molecular Mechanisms and Dietary Intervention.

Multiple Sclerosis (MS) is a chronic, inflammatory, neurodegenerative demyelinating disease of the central nervous system (CNS). Unfortunately, MS causes important disability in young adults and its prevalence is increasing. While the etiology of MS etiology is not completely understood, it seems to be a multifactorial entity that is influenced by both genetic and epigenetic modifications. Epigenetic mechanisms add or remove different chemical groups for the activation or inhibition of gene expression to block the production of proinflammatory proteins. It is truly important to identify the factors that can trigger epigenetic changes in MS to complement the therapeutic approach, prevent disability and improve patients quality of life. Here, we have conducted a review of external factors that influence in MS and their epigenetic mechanisms. For example, hypomethylation can promote changes in the myelin and subsequent autoimmune reactions. Therapeutic tools can be used, including the histone deacetylase inhibitor Trichostatin A, which ameliorates demyelinating diseases in rodents. However, drugs are not only the therapeutic option: recent studies have also evaluated the therapeutic potential of several bioactive dietary components in neurodegeneration and axonal dysfunction. Numerous food-derived molecules exert important metabolic actions. These molecules include plant polyphenols such as catechins and isoflavones, Ω-3 and Ω-6 polyunsaturated fatty acids, short-chain fatty acids, sulfur-containing compounds such as dally sulfide and other compounds. Antioxidant and anti-inflammatory components in the diet involve transcription factors as well. However, many external factors have shown to influence MS, although no specific epigenetic mechanisms are known. In this review, we gather both established and new evidences about the genetic, epigenetic and environmental factors influencing MS and the dietary components that could modulate MS relapse and progression.

Effect of Different Extrusion Parameters on Dietary Fiber in Wheat Bran and Rye Bran.

Wheat bran and rye bran are mostly used as animal feed today, but their high content of dietary fiber and bioactive components are beneficial to human health. Increased use of bran as food raw material could therefore be desirable. However, bran mainly contains unextractable dietary fiber and deteriorates the sensory properties of products. Processing by extrusion could increase the extractability of dietary fiber and increase the sensory qualities of bran products. Wheat bran and rye bran were therefore extruded at different levels of moisture content, screw speed and temperature, in order to find the optimal setting for increased extractability of dietary fiber and positive sensory properties. A water content of 24% for wheat bran and 30% for rye bran, a screw speed of 400 rpm, and a temperature of 130 °C resulted in the highest extractability of total dietary fiber and arabinoxylan. Arabinoxylan extractability increased from 5.8% in wheat bran to 9.0% in extruded wheat bran at those settings, and from 14.6% to 19.2% for rye bran. Total contents of dietary fiber and arabinoxylan were not affected by extrusion. Content of β-glucan was also maintained during extrusion, while its molecular weight decreased slightly and extractability increased slightly. Extrusion at these settings is therefore a suitable process for increasing the use of wheat bran and rye bran as a food raw material.

Linking Diet to Colorectal Cancer: The Emerging Role of MicroRNA in the Communication between Plant and Animal Kingdoms.

Environmental and lifestyle factors, including diet and nutritional habits have been strongly linked to colorectal cancer (CRC). Of note, unhealthy dietary habits leading to adiposity represent a main risk factor for CRC and are associated with a chronic low-grade inflammatory status. Inflammation is a hallmark of almost every type of cancer and can be modulated by several food compounds exhibiting either protective or promoting effects. However, in spite of an extensive research, the underlying mechanisms by which dietary patterns or bioactive food components may influence tumor onset and outcome have not been fully clarified yet. Growing evidence indicates that diet, combining beneficial substances and potentially harmful ingredients, has an impact on the expression of key regulators of gene expression such as the non-coding RNA (ncRNA). Since the expression of these molecules is deranged in chronic inflammation and cancer, modulating their expression may strongly influence the cancer phenotype and outcomes. In addition, the recently acquired knowledge on the existence of intricate inter-kingdom communication networks, is opening new avenues for a deeper understanding of the intimate relationships linking diet to CRC. In this novel scenario, diet-modulated ncRNA may represent key actors in the interaction between plant and animal kingdoms, capable of influencing disease onset and outcome. In this review, we will summarize the studies demonstrating a link between bioactive food components, including food-derived, microbiota-processed, secondary metabolites, and host ncRNA. We will focus on microRNA, highlighting how this plant/animal inter-kingdom cross-talk may have an impact on CRC establishment and progression.

Black Elderberry Extract Improves Serum HDL‐cholesterol and Paraoxonase‐1 Activity in Atherosclerosis‐Prone Mice

Cardiovascular disease (CVD) is one of the most prevalent non‐communicable diseases in the United States, claiming upwards of 600,000 lives per year. Excessive plaque accumulation and atherosclerosis are hallmark characteristics of this disease. HDL is responsible for the removal of deposited cholesterol in the arteries to the liver for excretion, a process known as reverse cholesterol transport. Low levels of high‐density lipoprotein cholesterol (HDL‐C) have been correlated with increased risk for CVD in epidemiological studies. However, drug therapies targeting an increase in HDL‐C have failed to demonstrate a reduced risk. More recently, HDL particle functionality rather than HDL‐C concentration, has been suggested to be a better predictor of CVD risk. Ingestion of bioactive dietary components, such as polyphenols, may increase both HDL‐C concentration and HDL functionality. Black elderberry (Sambucus nigra) contains high amounts of anthocyanins (~1300 mg/100 g), a subclass of polyphenols, relative to other edible berries. We investigated whether the consumption of a black elderberry extract (BEE) influenced lipid metabolism, HDL functionality, and atherosclerosis in a mouse model of hypercholesterolemia (apoE−/− mice). We fed male apoE−/− (n = 12/group) ad libitum a low‐fat AIN‐93M diet supplemented with 0 or 1% (by weight of diet; ~150 mg anthocyanins/kg body weight) black elderberry extract (BEE) for 24 weeks. Supplementation with BEE significantly increased total cholesterol (+31.5%) and HDL‐C (+26%), while triglycerides were not significantly changed. Paraoxonase‐1 (PON1), a lipolactonase enzyme carried by HDL which prevents lipoprotein oxidation, was increased by 32% in the treatment group when compared to controls (p < 0.05). Analysis of hepatic gene expression revealed that BEE significantly increased expression of Nrf2 and GClc (p < 0.05), both of which are important for endogenous antioxidant defense and may be related to the significant reductions in serum alanine transaminase and aspartate transaminase levels in the BEE treatment group (p < 0.05). Despite improved markers of HDL‐C and HDL function, en face analysis of aortas showed no difference in severity of atherosclerosis between groups. The current data suggests that BEE supplementation improves HDL‐C and HDL function, while also upregulating genes involved in the endogenous antioxidant defense.Support or Funding InformationSupported by the USDA National Institute of Food and Agriculture, #1005610 and Hatch project #1003343.

Effect of dietary bioactive compounds and biopolymer encapsulated lipids on metabolism of lipids in high fat diet‐fed mice

We determined the effects of dietary bioactive components and biopolymer encapsulation on lipids’ metabolism in obese mice. Six random groups of 60 obese mice were fed the following diets for 95 days: Basal diet; cellulose encapsulated lipids; pectin and chitosan encapsulated lipids (EPC); Camellia sinensis and Coix lacryma enhanced diet; C. sinensis and C. lacryma diet plus pectin and chitosan encapsulated lipids; hydroxycitric acid, caffeine, and vitamin C enhanced diet plus pectin and chitosan encapsulated lipids (HCVEPC). The HCVEPC group usually presented lower body and total adipose tissue weight compared to other diet groups. Intestine length was not significantly different among all dietary groups. Serum leptin, triglycerides, and cholesterol were significantly lower in EPC and HCVEPC compared to other groups. However, serum glucose was not significantly different among groups. Our results indicate that bioactive compounds and biopolymers’ encapsulation act synergistically in the reduction of body and adipose tissue weight.Practical applications: Based on the results described above, it is suggested that bioactive components and biopolymer encapsulation with some natural products has synergistically in the reduction of body and adipose tissue weight on in vivo models. In addition, through this results suggest that can be used information and utilization of lipids metabolism, this research is to highlight the potential of selected natural products as a source of functional Substances. Therefore, lipids encapsulated with dietary biopolymers and bioactive compounds should be considered as a prospective functional food and pharmaceuticals application.We investigated effects of dietary bioactive components and biopolymer encapsulation on lipids’ metabolism in obese mice. Bio‐active components could enhance the effect of body and adipose tissue weight reduction in obese mice models. Dietary bio‐active compounds together with biopolymer encapsulated lipids showed a great reduction in serum triglycerides and cholesterol.

Why Are Omics Technologies Important to Understanding the Role of Nutrition in Inflammatory Bowel Diseases?

For many years, there has been confusion about the role that nutrition plays in inflammatory bowel diseases (IBD). It is apparent that good dietary advice for one individual may prove inappropriate for another. As with many diseases, genome-wide association studies across large collaborative groups have been important in revealing the role of genetics in IBD, with more than 200 genes associated with susceptibility to the disease. These associations provide clues to explain the differences in nutrient requirements among individuals. In addition to genes directly involved in the control of inflammation, a number of the associated genes play roles in modulating the gut microbiota. Cell line models enable the generation of hypotheses as to how various bioactive dietary components might be especially beneficial for certain genetic groups. Animal models are necessary to mimic aspects of the complex aetiology of IBD, and provide an important link between tissue culture studies and human trials. Once we are sufficiently confident of our hypotheses, we can then take modified diets to an IBD population that is stratified according to genotype. Studies in IBD patients fed a Mediterranean-style diet have been important in validating our hypotheses and as a proof-of-principle for the application of these sensitive omics technologies to aiding in the control of IBD symptoms.

Combinatorial epigenetic mechanisms and efficacy of early breast cancer inhibition by nutritive botanicals.

Aim:Aberrant epigenetic events are important contributors to the pathogenesis of different types of cancers and dietary botanicals with epigenetic properties can influence early cancer development leading to cancer prevention effects. We sought to investigate potential combinatorial effects of bioactive dietary components including green tea polyphenols (GTPs) and broccoli sprouts (BSp) on neutralizing epigenetic aberrations during breast tumorigenesis.Materials & methods:The combinatorial effects were evaluated in a breast cancer transformation cellular system and breast cancer mouse xenografts.Results & conclusion:Combined treatment with epigallocatechin-3-gallate in GTPs and sulforaphane in BSp resulted in a synergistic inhibition of breast cancer cellular growth. Further studies revealed this combination led to genome-wide epigenetic alterations. Combinatorial diets significantly inhibited tumor growth in breast cancer mouse xenografts. Collectively, these studies indicate that combined GTPs and BSp are highly effective in inhibiting early breast cancer development by, at least in part, regulating epigenetic mechanisms.

Effects of Food Matrix and Western Diet on Colorectal Cancer and Metabolism in C57BL/6 Mice

Rodent studies that test the effects of bioactive dietary components on chronic disease generally use purified basal diets that were established using nutrient profiles optimized for growth and fertility such as the AIN93‐G diet which is not reflective of typical American macro and micronutrient intake. Previously, we used NHANES data to formulate the Total Western Diet (TWD), a rodent diet that emulates average American intake levels for macro and micronutrients using nutrient density. We have demonstrated that the TWD increases basal colorectal cancer (CRC) by ~2‐fold compared to the standard AIN93‐G diet in three different models. However, the TWD is comprised of purified ingredients and does not recapitulate the complex food matrix consumed by Americans. Using the latest version of the USDA Food Intakes Converted to Retail Commodities Database, we modified the TWD by using the most commonly consumed whole foods as dietary ingredients. The TWD2 has the same micro and macronutrient content as the previous TWD but contains 25 food ingredients including: sweeteners (sucrose and high fructose corn syrup); flours (wheat, potato, corn, rice and oat); meats (beef, poultry, pork, eggs and fish); dairy (lactose, whey and casein); fruits/vegetables (oranges, apples, bananas, grapes, strawberries, soy, snap beans, peanuts and cashews). To test whether food matrix affected CRC outcome, mice were fed either: AIN93‐G, TWD, TWD2 or TWD‐F (TWD matched for total fiber to TWD2). Mice were initiated with 10 mg/kg azoxymethane (AOM) and provided 1% dextran sodium sulfate (DSS) in their drinking water for 4 weeks to promote colonic inflammation and tumorigenesis. Necropsy and tumor assessment was performed after 15 weeks of treatment. A cohort of sham mice were fed the same diets but not injected with AOM or given DSS to determine effect of diets on metabolism and the gut microbiome. Mice fed the TWD, TWD‐F, and TWD2 did not differ in colon tumor multiplicity but all had significantly more tumors than mice fed the AIN93‐G diet (P < 0.01). Interestingly, mice fed the TWD2 diet consumed significantly more energy and gained more weight than all other treatments (P < 0.05). TWD2 fed mice also had significantly higher total fat mass, body fat percentage, and total visceral fat mass than AIN93‐G fed mice (P < 0.05) but not the purified TWD treatments. Lastly, TWD2 fed mice had significantly higher blood glucose concentrations than AIN93‐G fed mice, but not mice fed the purified TWD diets, at 90 and 120 minutes after oral glucose challenge (P < 0.05). Our data suggests that a diet with a complex food matrix, compared to purified diets with the same macro and micronutrient content, does not influence CRC but does increase energy consumption, adiposity, and glucose intolerance.Support or Funding InformationUtah Agricultural Experiment Station

Biotransformation of 5‐demethyltangeretin in mice: generation of anti‐cancer metabolites

5-demethyltangeretin (5DT) is a unique polymethoxyflavone isolated from citrus peels, and showed potent inhibitory effects on various human cancer cells. Biotransformation of dietary bioactive components plays critical roles in their biological activities because they can be transformed to metabolites with significant bioactivities. In this study, we determined the metabolic fate of 5DT in mice and determined the bioactivities of its major metabolite. Our results showed that 5DT underwent extensive biotransformation in mice fed with 5DT, and produced demethylated and hydroxylated metabolites via phase I metabolism, and glucuronide and sulfate conjugates via phase II metabolism. In particular, 4′ position on the B-ring of 5DT was the primary site for demethylation (formation of xanthomicrol, XAN), and 3′ position on the B-ring was the primary site for hydroxylation (formation of 5, 3′, 4′-tridemethyltangeretin, TDT). Interestingly, the colonic levels of XAN was much higher than that of 5DT. Therefore, we further investigated the inhibitory effects of XAN on human colon cancer cells. It was found that XAN significantly inhibited the colon cancer cell growth by inducing G2 phase cell cycle arrest and cellular apoptosis. Overall, this study identified major metabolites of 5DT, and demonstrated the anti-cancer potential of the metabolites. Support or Funding Information This study was supported by funding from USDA. Biotransformation pathways of 5-demethyltangeretin (5DT)

High-Molecular-Weight Proanthocyanidins in Foods: Overcoming Analytical Challenges in Pursuit of Novel Dietary Bioactive Components.

Proanthocyanidins (PACs) are an abundant but complex class of polyphenols found in foods and botanicals. PACs are polymeric flavanols with a variety of linkages and subunits. Connectivity and degree of polymerization (DP) determine PAC bioavailability and bioactivity. Current quantitative and qualitative methods may ignore a large percentage of dietary PACs. Subsequent correlations between intake and activity are hindered by a lack of understanding of the true PAC complexity in many foods. Additionally, estimates of dietary intakes are likely inaccurate, as nutrient databank values are largely based on standards from cocoa (monomers to decamers) and blueberries (mean DP of 36). Improved analytical methodologies are needed to increase our understanding of the biological roles of these complex compounds.

Diet and Inflammation: Possible Effects on Immunity, Chronic Diseases, and Life Span

Chronic inflammation negatively impacts all physiological functions, causing an array of degenerative conditions including diabetes; cancer; cardiovascular, osteo-articular, and neurodegenerative diseases; autoimmunity disorders; and aging.In particular, there is a growing knowledge of the role that gene transcription factors play in the inflammatory process. Obesity, metabolic syndrome, and diabetes represent multifactorial conditions resulting from improper balances of hormones and gene expression. In addition, these conditions have a strong inflammatory component that can potentially be impacted by the diet. It can reduce pro-inflammatory eicosanoids that can alter hormonal signaling cascades to the modulation of the innate immune system and gene transcription factors. Working knowledge of the impact of how nutrients, especially dietary fatty acids and polyphenols, can impact these various molecular targets makes it possible to develop a general outline of an anti-inflammatory diet that offers a unique, nonpharmacological approach in treating obesity, metabolic syndrome, and diabetes.Several important bioactive dietary components can exert their effect through selected inflammatory pathways that can affect metabolic and genetic changes. In fact, dietary components that can modulate glucose and insulin levels, as well as any other mediator that can activate nuclear factor-kB, can also trigger inflammation through common pathway master switches.

The anti‐inflammatory effects of wheat germ oil on lipopolysaccharide‐activated human monocytic (THP‐1) cells

Heart disease is the leading cause of death worldwide, and the underlying pathological feature is atherosclerosis. Atherosclerosis is an inflammatory disease involving various immune cells with macrophages as key agents in the disease progression. Macrophages play a vital role by secreting pro‐inflammatory cytokines including tumor necrosis factor (TNF)‐α and interleukin (IL)‐6. Dietary bioactive components are being studied for their beneficial properties. Wheat germ oil (WGO) is rich in omega‐3 fatty acids, vitamin E and policosanols which have been reported to have pharmacological effects, including anti‐inflammatory property. The present study investigated the anti‐inflammatory effects of different WGOs on cytokine production in lipopolysaccharide (LPS)‐activated THP‐1 cells. THP‐1 cells were treated with different concentrations (0‐1.5 x 10‐3%) of four WGOs prepared using different extraction methods (WGO1, WGO2, WGO3 and WGO4) and one commercial WGO (WGOC) with or without LPS (100 ng/mL) for 24 h. None of the WGOs tested induced cell death or had a negative effect on cell proliferation. All WGOs significantly decreased TNF‐α and IL‐6 production (P<0.0001), with WGOC being the most effective. In contrast, unrefined hexane extracted WGO4 induced the highest production of the anti‐inflammatory cytokine, IL‐10.These findings suggest that WGOs can simultaneously down regulate pro‐inflammatory and up‐regulate anti‐inflammatory responses and that the extraction method influences the magnitude of these effects.

Diet-based strategies for cancer chemoprevention: the role of combination regimens using dietary bioactive components.

Chemopreventive agents that the general population can consume for prolonged periods of time with minimal risk of any side effects are of great interest to all in search of a solution to the pervasive incidence of cancer. Dietary bioactive components have been found to modulate many deregulated molecular pathways associated with the initiation and progression of different types of cancer. Combination regimens with dietary bioactive components are a promising strategy for cancer chemoprevention because they may offer enhanced protective effects against cancer development but cause little or no adverse effects. This article provides an overview of studies examining the combination of dietary bioactive components for the chemoprevention of major types of cancer. A better understanding of existing research on the combination of dietary bioactive components will provide an important basis for the rational design of future combination studies and the successful development of cancer chemoprevention strategies.

Dietary Bioactives: Establishing a Scientific Framework for Recommended Intakes

In the United States, dietary reference intakes describe the relations between nutrient intakes and indicators of adequacy, prevention of disease, and avoidance of excessive intakes among healthy populations for essential nutrients but not dietary bioactive components (DBCs), whose absence from the diet is presumably not deleterious to health (i.e., does not cause a deficiency syndrome). An appropriate framework is needed for establishing recommended intakes for which public health messages and food labeling for DBCs can be derived, because their putative health benefits may not be readily defined in the context of nutritional essentiality. In addition, a myriad of factors make determining their intake and status and investigating their discrete contributions to health particularly challenging. Therefore, the ASN Dietary Bioactive Components Research Interest Section felt it worthwhile to convene a special "hot topic" session at the 2014 Experimental Biology meeting to discuss this issue and serve as a call for future scientific dialogue on establishing a framework for recommended intakes of DBCs. This session summary captures the discussions and presentations that transpired during this session.

Epigenetic nutraceutical diets in Alzheimer's disease.

There is growing support that environmental influences and individual genetic susceptibility may increase the incidence and accelerate the onset of Alzheimer's disease (AD). Epigenetic mechanisms encompass a complex regulatory network of modifications with considerable impact on health and disease risk. Abnormal epigenetic regulation is a hallmark in many pathological conditions including AD. It is well recognized that numerous bioactive dietary components mediate epigenetic modifications associated with the pathophysiology of several diseases. Although the influences of dietary factors on epigenetic regulation have been extensively investigated, only few studies have explored the effects of specific food components in regulating epigenetic patterns during neurodegeneration and AD. Epigenetic nutritional research has substantial potential for AD and may represent a window of opportunity to complement other interventions. Here, we provide a brief overview of the main mechanisms involved in AD, some of which may be epigenetically modulated by bioactive food.

Effects of resveratrol and alcohol on mood and cognitive function in older individuals

BACKGROUND: Heavy consumption of alcohol has well-documented negative effects on cognition both acutely and chronically. Nevertheless there is increasing research interest in the potential positive influence on cognitive functioning of bioactive dietary components including resveratrol from red wine. OBJECTIVE: This study aimed to compare the acute mood and cognitive effects of resveratrol-enriched red wine with red wine alone during mentally effortful tasks in older individuals. METHODS: Following a double-blind, balanced, crossover design, 16 volunteers (mean age 70.44 years) were tested on two occasions, once following 100 ml red wine; once following the same beverage but enriched with 200 mg resveratrol. They completed six cycles of the Cognitive Demand Battery comprising Serial Threes, Serial Sevens, a Rapid Visual Information Processing task and ratings of mental fatigue. Serum resveratrol and metabolites were co-monitored. RESULTS: There were differential cognitive effects of the two treatments. Compared with red wine alone, resveratrol-enriched wine was associated with significantly enhanced Serial Sevens performance. Conversely red wine alone resulted in better performance during Serial Threes. Serum analysis confirmed absorption of resveratrol metabolites. CONCLUSIONS: These results may reflect increased blood flow from resveratrol and the documented stimulant effects of alcohol at low levels. However the study requires replication with an inert control group.

Are dietary bioactives ready for recommended intakes?

Research has shown that numerous dietary bioactive components that are not considered essential may still be beneficial to health. The dietary reference intake (DRI) process has been applied to nonessential nutrients, such as fiber, yet the majority of bioactive components await a recommended intake. Despite a plethora of new research over the past several years on the health effects of bioactives, it is possible that the field may never reach a point where the current DRI framework is suitable for these food components. If bioactives are to move toward dietary guidance, they will likely require an alternative path to get there.

Epigenetics: A New Link Between Nutrition and Cancer

Emerging studies suggest that dietary components can affect gene expression through epigenetic mechanisms. Epigenetic modifications are heritable and potentially reversible changes in gene expression that do not require changes in the DNA sequence. The main mechanisms of epigenetic control in mammals are DNA methylation, histone modifications, and RNA silencing. The potential reversibility of epigenetic changes suggests that they could be modulated by nutrition and bioactive food compounds. Thus, epigenetic modifications could mediate environmental signals and provide a link between susceptibility genes and environmental factors in the etiology of cancer. Elucidating the impact of nutrition on epigenetic mechanisms may serve as a tool to predict an individuals’ susceptibility to cancer, provide dietary recommendations, or provide therapeutic applications of natural compounds against cancer. The optimal duration and the dose necessary for a chemopreventive effect require further studies. There is limited information about tissue specificity and temporal aspects of dietary treatments. Species differences need to be considered when interpreting results from various models. Importantly, molecular mechanisms of bioactive dietary components should be investigated in greater detail in human intervention studies. Although some of these issues remain controversial, this review mainly focuses on promising data that support the developing field of Nutritional Epigenetics.

Desarrollo de una pasta untable vegetal a base de harina de grano entero de sorgo y mijo

Introducción: las guías nutricionales recomiendan incrementar el consumo de productos a base de cereales de grano entero por su contenido en fibras y biocomponentes. Dado que las propiedades sensoriales y tecnológicas de estos productos constituyen aún un desafío se desarrolló una pasta untable tipo “paté”, apta para la creciente población celíaca.Material y Métodos: se obtuvieron harinas de grano entero de sorgo y mijo (100% de extracción), los ingredientes restantes se evaluaron y seleccionaron en función de las características deseadas. De la pasta se analizó la composición centesimal, propiedades sensoriales y vida útil.Resultados: los ingredientes seleccionados fueron (en %): margarina (16,7), proteína de soja (25,1), almidón pre-gelatinizado (2,7), ácido ascórbico (2,0), azúcar (1,1), sal (1,1), propionato de calcio (0,3) y goma xántica (0,5). La vida útil se siguió durante 28 días a través de (i) análisis de químicos de: humedad (varió entre 55,5 a 51,3%, p-value = 0,000741), índices de peróxidos (<0,1 meqO2/Kg) y de acidez (de 4,3 a 6,2 mg K(OH)/g); (ii) recuentos microbiológicos de: clostridios sulfito reductores, Escherichia coli y Salmonella spp (ausencia), mesófilos aerobios, coliformes totales, Staphylococcus aureus y mohos y levaduras cuyos recuentos fueron siempre menores a 100; (iii) evaluación sensorial (aceptable).Conclusiones: se obtuvo una pasta untable 100% vegetal con características organolépticas similares a las de un paté, estable durante al menos 28 días. Tiempo durante el cual se mantuvieron los parámetros químicos y microbiológicos dentro de los rangos aceptables por la legislación argentina y sin modificaciones negativas en la percepción sensorial.