Dietary Treatments Research Articles

Effect of dietary supplementation of xylanase alone or combination of xylanase and β-glucanase on growth performance, meat quality, intestinal measurements, and nutrient utilization in broiler chickens.

The current study aimed to investigate the effect of dietary supplementation of xylanase alone or combination of xylanase and β-glucanase in high non-starch polysaccharides (NSP) diets with low energy on growth performance, meat quality, intestinal measurements, stress responses, and energy and nutrient utilization in broiler chickens. A total of four hundred 8-d-old Ross 308 broiler chickens were randomly allotted to 1 of 4 treatment groups with 10 replicates. A positive control (PC) diet was formulated with adequate energy and nutrients, whereas a negative control (NC) diet had 100 kcal/kg less AMEn than the PC diet with increasing inclusion of high NSP ingredients. Two additional diets were produced by supplementing 0.1% xylanase alone or 0.1% xylanase and β-glucanase mixture in the NC diet. Experiments lasted for 27 d. Birds fed PC diets had less (p<0.05) FCR than those fed NC diets. Birds fed NC diets supplemented with xylanase alone or combination of xylanase and β-glucanase had less (p<0.05) FCR than those fed NC diets. Dietary supplementation of xylanase alone in NC diets exhibited the highest (p<0.05) breast meat pH among dietary treatments. Birds fed PC diets or NC diets supplemented with xylanase and β-glucanase combination exhibited greater (p<0.05) VH:CD ratio than those fed NC diets. Dietary supplementation of xylanase alone and combination of xylanase and β-glucanase in NC diets decreased (p<0.05) ileal digesta viscosity and increased (p<0.05) xylo-oligosaccharide concentrations in the gastrointestinal tract (GIT) compared with NC diets without affecting energy and nutrient utilization in NC diets. Dietary supplementation of xylanase in high NSP diets with low energy concentrations improves growth performance by decreasing digesta viscosity and increasing concentrations of xylo-oligosaccharides in the GIT of broiler chickens. However, little additional benefits of β-glucanase supplementation in combination with xylanase are identified for broiler chickens.

Optimizing tropical dairy goat diets: balancing rumen degradable protein, non-fiber carbohydrates, and sulfur requirements.

This study aimed to investigate the effects of rations incorporating rumen degradable protein (RDP), non-fiber carbohydrate (NFC), and sulfur on nutrient utilization, milk production, milk quality, and the economic aspects of dairy goats. In the first study, five treatments were tested in a block-randomized design to examine in vitro fermentability and digestibility. Treatments included P0 (control diet), P1 (P0 + 7.5% cassava-NFC), P2 (P0 + 7.5% cassava-NFC and 5% soybean), P3 (P0 + 7.5% cassava-NFC and 5% autoclaved soybean), and P4 (P0 + 7.5% cassava-NFC, 5% autoclaved soybean, and 0.1% sulfur). In the second study, sixteen lactating Saanen-Ettawa crossbreed dairy goats (initial milk production = 0.97 ± 0.25 L/head/day, 30 DIM; body weight = 44.44 ± 7.20 kg) were assigned into four groups and fed treatment diets: R0 (basal diet), R1 (R0 + 12% autoclaved soybean), R2 (R0 + 12% autoclaved soybean and 9% cassava-NFC), and R3 (R0 + 12% autoclaved soybean, 9% cassava-NFC, and 0.11% sulfur). The diets were offered for 7 weeks with a two-week adaptation period. Parameters observed include milk production and quality, milk fatty acids, blood hematology and metabolites, and economic aspects. The study used a block randomized design with initial weight as a block. The treatment diets in the first study had no effect on in vitro fermentability and digestibility. Treatments R2 and R3 resulted in higher milk production than R0 and R1. Milk quality remained consistent across treatments, while solid non-fat, lactose, and protein was higher in R2 and R3. Blood hematology was unaffected by the treatments. Nutrient efficiency and income over feed cost were enhanced by R2 and R3 treatments. Protected RDP using autoclaved soybean and cassava-NFC maintained in vitro digestibility, even though it did not improve in vitro fermentability. Precision dairy ration based on RDP, NFC, and sulfur positively impacts milk production, nutrient efficiency, and animal health in dairy goats.

Reproductive tract morphometry of Hyla rabbits fed diet containing graded levels of watermelon rind and wheat offal as absorbent

The evaluation of breeding and fertility potential of animals demands understanding of basic morphometric characteristics of their reproductive organs which, among other factors, may be affected by nutrition. This study was conducted to assess the effects of feeding graded levels of watermelon rind and wheat offal as absorbent on morphometric characteristics of the reproductive organs of Hyla rabbits. Forty-five Hyla rabbits of both sexes (15 bucks and 30 does) with nine animals per treatment (3 bucks and 6 does in each treatment) in a completely randomized design were randomly assigned to the dietary treatment containing 0 %, 10 %, 20 %, 30 %, and 40 % of watermelon rind and wheat offal. The data collected included weight and length of the reproductive tract of the rabbits. The morphometric characteristics of reproductive tract of the rabbits were not significantly affected by the dietary treatment, however, there were significant effect (p &gt; 0.05) of the dietary treatment on the weight of the left oviduct, paired oviduct, and the paired ovary of the rabbits. The experimental diets significantly affected (p &lt; 0.05) penis length and width, the right spermatic cord and the length of the right scrotal. The diets had no significant effect (p&lt;0.05) on the weight of reproductive tracts of buck except on the paired spermatic cord. The experimental diets can be a good alternative for the highly competitive and costly grains in the diet of Hyla rabbits without any adverse effect on the growth of reproductive organs and consequently on the fertility and other activities related to reproduction of Hyla rabbits. Keywords: morphology, rabbits, reproduction, watermelon rind, wheat offal

Standardized Ileal Digestibility of Amino Acids in Hybrid Rye Ground to Two Particle Sizes and Fed With or Without Multienzyme Supplement to Young Growing Pigs.

A newly developed hybrid rye with enhanced ergot resistance has potential as an alternative energy source for pigs. An experiment was conducted to evaluate the effects of particle size (PS) and multienzyme supplement (MES) on the apparent total tract digestibility (ATTD) of energy and nutrients and the standardized ileal digestibility (SID) of amino acids (AA) in hybrid rye fed to growing pigs. Bono and Gatano, two hybrid rye varieties, were ground to either a coarse (1111 and 1210 μm) or a fine (594 and 717 μm) PS using a hammer mill. Eighteen ileal-cannulated barrows (initial BW = 18.2 ± 1.0 kg) were randomly assigned to 1 of 9 dietary treatments in a replicated 9 × 3 incomplete Latin square design to give six observations per treatment. Among the nine experimental diets, eight featured two hybrid rye varieties (Bono or Gatano), either coarsely or finely ground, as the only source of AA with or without MES, while an N-free diet was used to estimate the endogenous losses of AA at the distal ileum. All diets contained titanium dioxide as an indigestible marker. Each period lasted 9 days, with the first 5 days being for adaptation followed by 2 days each for faecal and ileal digesta collection. Data were analysed using the MIXED procedure of SAS, with the final model having variety, PS, MES, their two-way interactions, and a three-way interaction. No interactions were noted except that the apparent ileal digestibility and SID of crude protein (CP) and most AA in Gatano had greater effects of MES than Bono (Variety × MES; p < 0.05). The ATTD of dry matter (DM), gross energy (GE) and CP were greater (p < 0.05) in Bono diets than in Gatano diets. Finely ground hybrid rye diets had higher (p < 0.05) ATTD of neutral detergent fibre assayed with a heat stable amylase and expressed inclusive of residual ash (NDF) and ether extract compared to coarsely ground hybrid rye diets. Dietary MES supplementation increased (p < 0.05) the ATTD of DM, GE, CP, NDF, acid detergent fibre expressed inclusive of residual ash, and ash in hybrid rye diets. In conclusion, the nutrients digestibility of hybrid rye can be affected by its variety. PS reduction improved the nutrient digestibility in hybrid rye, and MES supplementation improved the energy and AA digestibility in hybrid rye fed to growing pigs.

Element Changes Occurring in Brain Point at the White Matter Abnormalities in Rats Exposed to the Ketogenic Diet During Prenatal Life.

A large number of clinical studies demonstrate that the ketogenic diet (KD) may be an effective approach to the reduction of epileptic seizures in children and adults. Such dietary therapy could also help pregnant women with epilepsy, especially since most antiseizure drugs have teratogenic action. However, there is a lack of medical data, considering the safety of using KD during gestation for the progeny. Therefore, we examined the influence of KD used prenatally in rats on the elemental composition of the selected brain regions in their offspring. For this purpose, synchrotron radiation-induced X-ray fluorescence (SR-XRF) microscopy was utilized, and elements such as P, S, K, Ca, Fe, and Zn were determined. Moreover, to verify whether the possible effects of KD are temporary or long-term, different stages of animal postnatal development were taken into account in our experiment. The obtained results confirmed the great applicability of SR-XRF microscopy to track the element changes occurring in the brain during postnatal development as well as those induced by prenatal exposure to the high-fat diet. The topographic analysis of the brains taken from offspring of mothers fed with KD during pregnancy and appropriate control individuals showed a potential influence of such dietary treatment on the brain levels of elements such as P and S. In the oldest progeny, a significant reduction of the surface of brain areas characterized by an increased P and S content, which histologically/morphologically correspond to white matter structures, was noticed. In turn, quantitative elemental analysis showed significantly decreased levels of Fe in the striatum and white matter of 30-day-old rats exposed prenatally to KD. This effect was temporary and was not noticed in adult animals. The observed abnormalities may be related to the changes in the accumulation of sphingomyelin and sulfatides and may testify about disturbances in the structure and integrity of the myelin, present in the white matter.

Practices and Barriers in Implementing the Low FODMAP Diet for Irritable Bowel Syndrome Among Malaysian Dietitians: A Qualitative Study

The low fermentable oligo-, di-, mono-saccharides and polyols (FODMAP) diet (LFD) is a second-line dietary intervention for irritable bowel syndrome (IBS) patients, involving FODMAP restriction, reintroduction, and personalization, and it needs to be delivered by dietitians. However, the application of this diet among Malaysian IBS patients is not well understood. This study aimed to explore the practices and barriers in delivering the LFD among Malaysia dietitians. Semi-structured qualitative interviews were conducted online with practicing dietitians until the data reached saturation. All the interview sessions were audio recorded and transcribed verbatim. Thematic analysis was used to analyze the data. Eleven dietitians were interviewed, with 36.4% (n = 4) having more than 10 years of experience. The following four themes regarding their practices emerged: 1. dietary advice on FODMAP restriction; 2. duration of FODMAP restriction phase; 3. references used to get information about FODMAPs, and 4. strategies on reintroduction. Meanwhile, the following seven barriers were identified: 1. lack of culturally relevant educational materials; 2. limited knowledge about the LFD; 3. inadequate formal training among dietitians; 4. lack of integration in multi-disciplinary care; 5. low health literacy of patients; 6. low compliance rate among patients, and 7. restrictions for certain populations. LFD implementation in Malaysia is not standardized as only experienced dietitians can provide dietary evidence-based advice. Lack of training and culturally specific resources are some of the main barriers that were identified to be limiting the implementation of the diet. Therefore, there is a need for training programs and resource development to support Malaysian dietitians in managing IBS patients.

Pregnancy and nutrition: a comprehensive review

Nutrition plays a critical role in ensuring a healthy pregnancy and optimizing outcomes for both the mother and the foetus. This review explores key aspects of pregnancy nutrition, including the importance of essential nutrients such as folic acid, iron, calcium, and omega-3 fatty acids. It examines the impact of maternal dietary patterns on fetal development and highlights the role of specific dietary interventions in managing conditions like gestational diabetes. The review also addresses the importance of nutritional supplements and food safety practices to prevent foodborne illnesses. Additionally, cultural and socioeconomic factors influencing prenatal nutrition are discussed, along with the significance of hydration and the effects of maternal weight on pregnancy outcomes. By integrating current research and guidelines, this review aims to provide a comprehensive understanding of how nutrition affects pregnancy and offers insights for improving prenatal care and health outcomes.

Feasibility of the MIND+SOUL Culturally Tailored Brain Healthy Diet: A Pilot Study

Alzheimer’s disease (AD) disproportionately impacts Black Americans, who are three times more likely to develop AD. While heart-healthy diets have shown potential in reducing AD risk, research on adapted dietary interventions for Black American communities remains limited. This pilot study assessed the feasibility and acceptability of an adapted brain healthy diet intervention (MIND + SOUL) and explored changes in cardiometabolic risk and cognition. Twenty-nine participants completed the 12-week intervention, which included culturally tailored health education, cooking classes, health coaching, and groceries. Feasibility was assessed by attendance and retention rates, while acceptability was measured by two questionnaires. Participants had a mean age of 70.3, with 10.3% male. The intervention demonstrated high feasibility (89.3% retention) and acceptability (mean = 71.9, SD = 8.59), with meaningful improvements in body mass index (estimate = −0.54, P = 0.009), dietary intake (estimate = 28.39, P = 0.042), and executive function (estimate = 3.32, P &lt; 0.001). However, no significant changes in blood-based biomarkers were observed. The MIND + SOUL intervention demonstrated high feasibility and acceptability, improvements in body composition, cognitive function, and dietary behaviors, despite no significant changes in blood-based biomarkers. Findings suggest potential benefits for reducing AD risk factors and promoting healthy aging. Clinical Trials Registry: ClinicalTrials.Gov; NCT05414682.

Protective Effect of Extra Virgin Olive Oil on Cancers, Gastrointestinal Cancers, and All-Cause Mortality: A Competing Risk Analysis in a Southern Italian Cohort

Background/Objectives: This study investigates the association between extra virgin olive oil (EVOO) consumption and mortality risk in a cohort of Italian adults adhering to the Mediterranean diet. Methods: In a 17-year follow-up study involving participants from Castellana Grotte, Italy (2005–2023), we analyzed dietary intake and mortality data. Participants were categorized into three EVOO consumption groups: &lt;30 g/day, 30–50 g/day, and &gt;50 g/day. Mortality Hazard Ratios (HR) and Subdistribution Hazard Ratios (SHR) were calculated to assess the relationship between EVOO intake and all-cause and cancer mortality. Results: Higher EVOO consumption was associated with significantly reduced cancer and all-cause mortality. Specifically, the daily intake of 30–50 g of EVOO was linked to a 24% lower risk of all-cause mortality (HR 0.77; 95% CI 0.63–0.93), while the consumption of more than 50 g/day was associated with a 20% reduction (HR 0.80; 95% CI 0.65–0.98). The most pronounced benefit was observed for gastrointestinal cancers, with a 60% lower mortality risk for those consuming over 50 g/day (SHR 0.39; 95% CI 0.21–0.73). A 50% reduction in mortality risk from other cancers was also noted for the highest consumption category (SHR 0.50; 95% CI 0.31–0.81). Conclusions: The findings support the beneficial role of EVOO in reducing cancer mortality, particularly with higher consumption levels. The results underscore EVOO’s potential as a dietary intervention for cancer prevention, aligning with the Mediterranean diet’s overall health benefits. Further research is needed to confirm these findings and explore the underlying mechanisms.

Retinol intake and PCOS management: a plasma metabolite and protein analysis via Mendelian randomization and NHANES 2011–2016

BackgroundPolycystic Ovary Syndrome (PCOS) represents a complex endocrine disorder characterized by a significant interplay with metabolic dysfunction and obesity. This research endeavors to elucidate the causal dynamics among plasma metabolites, proteins, and PCOS, alongside Body Mass Index (BMI), to pinpoint prospective therapeutic interventions.MethodsThis investigation employed Mendelian randomization (MR) analyses combined with data derived from the National Health and Nutrition Examination Survey (NHANES) to explore the relationships between 1,400 plasma metabolites and PCOS, factoring in BMI adjustments. Additionally, the study examined the influence of plasma proteins and performed a retrospective cross-sectional analysis focusing on retinol consumption and testosterone levels.ResultsMR analyses showed metabolite Glycosyl-N-(2-hydroxynervonoyl)-sphingosine (GNS) and protein Keratin 19 (KRT19) were identified as significant markers in the context of PCOS and BMI adjustments. A Phenome-Wide Association Study (PheWAS) underscored the linkage between KRT19 and BMI, while gene-drug interaction findings demonstrated a connection between KRT19 and retinol. Analysis for NHANES data disclosed a negative correlation between retinol intake and testosterone levels, particularly within normal weight and obese cohorts, suggesting the feasibility of dietary interventions for PCOS management.ConclusionThe study sheds light on the intricate interactions between plasma metabolites, proteins, and PCOS, considering BMI variations, and highlights KRT19 protein as a promising therapeutic target. The outcomes support the integration of retinol consumption into dietary strategies to regulate testosterone levels and potentially alleviate PCOS symptoms, underscoring the necessity for personalized nutritional and therapeutic approaches in the effective management of PCOS.

Metaproteomics reveals diet-induced changes in gut microbiome function according to Crohn’s disease location

BackgroundCrohn’s disease (CD) is characterized by chronic intestinal inflammation. Diet is a key modifiable factor influencing the gut microbiome (GM) and a risk factor for CD. However, the impact of diet modulation on GM function in CD patients is understudied. Herein, we evaluated the effect of a high-fiber, low-fat diet (the Mi-IBD diet) on GM function in CD patients. All participants were instructed to follow the Mi-IBD diet for 8 weeks. One group of CD patients received one-time diet counseling only (Gr1); catered food was supplied for the other three groups, including CD patients (Gr2) and dyads of CD patients and healthy household controls (HHCs) residing within the same household (Gr3-HHC dyads). Stool samples were collected at baseline, week 8, and week 36, and analyzed by liquid chromatography-tandem mass spectrometry.ResultsAt baseline, the metaproteomic profiles of CD patients and HHCs differed. The Mi-IBD diet significantly increased carbohydrate and iron transport and metabolism. The predicted microbial composition underlying the metaproteomic changes differed between patients with ileal only disease (ICD) or colonic involvement: ICD was characterized by decreased Faecalibacterium abundance. Even on the Mi-IBD diet, the CD patient metaproteome displayed significant underrepresentation of carbohydrate and purine/pyrimidine synthesis pathways compared to that of HHCs. Human immune-related proteins were upregulated in CD patients compared to HHCs.ConclusionsThe Mi-IBD diet changed the microbial function of CD patients and enhanced carbohydrate metabolism. Our metaproteomic results highlight functional differences in the microbiome according to disease location. Notably, our dietary intervention yielded the most benefit for CD patients with colonic involvement compared to ileal-only disease.9orssYZV9GnhTtyZm1JCtCVideo

The development and testing of a single-arm feasibility and acceptability study of a whole foods diet intervention for adults with prediabetes and their offspring

BackgroundDiet is considered a first-line treatment option for prediabetes, a condition that affects 96 million United States (U.S.) adults. Diet patterns that prioritize whole foods (e.g., Mediterranean) are currently recommended to treat prediabetes. However, no studies have tested whether a U.S.-style diet pattern that prioritizes whole foods can be used to treat prediabetes. The purpose of this study was to assess the feasibility and acceptability of a whole foods diet for adults with prediabetes and their offspring prior to conducting a larger clinical trial.MethodsA 2-week single-arm pre-post experimental controlled-feeding intervention of a 2020–2025 Dietary Guidelines for Americans adapted whole foods diet (e.g., primarily focused on foods that have undergone limited processing or refinement) was conducted in adults (25–59 years) with prediabetes and their biological offspring (6–17 years). Families received 2 weeks of menus and grocery delivery and weekly counseling by a registered dietitian. Families were invited to attend an optional focus group session. Feasibility was based on having a ≥ 50% family completion rate with ≥ 80% completion of study outcomes. Measures included adult–child anthropometrics (weight [kg], body mass index [BMI] including BMI% and Z-scores for offspring, waist circumference [cm]) and child diet quality estimated using the 2015 Healthy Eating Index (HEI) from a single random food record. Wilcoxon signed rank was used to compare differences between baseline and 2-week anthropometrics measures and offspring total HEI scores. Qualitative data were analyzed using thematic analysis to understand factors attributed to diet adherence and acceptability.ResultsEight families enrolled (n = 8 adults, n = 12 offspring), with 7 families completing the study (12% attrition) and completing 100% of study outcome measures. Adults experienced a median weight loss of − 1.0 kg from baseline to 2 weeks (79.5 kg to 78.5 kg). Offspring had a 24-point increase in total 2015 HEI scores (median difference 50 to 74). Focus group participants (n = 4 adults) reported being satisfied with the program and expressed a willingness to continue the diet despite identified barriers.ConclusionsA whole foods diet that provides dietary support was found to be feasible and acceptable for families at risk for T2D. Future studies are needed to test the effects of the diet on prediabetes outcomes, diet quality, and diet adherence in adults and families.Trial registrationNCT05483972 at ClinicalTrials.gov. Registered July 25, 2022. https://clinicaltrials.gov/study/NCT05483972?cond=prediabetes&term=whole%20foods%20&rank=1

Microbiological and Physicochemical Approach in the Feeding of Superworm (Zophobas morio) with Petroleum-Derived Polymer Diets

Plastics are very versatile materials that have contributed to the development of society since the 19th century; however, their mismanagement has led to an accumulation of plastic waste in almost every ecosystem, affecting the fauna of the planet. However, recently, some studies have shown that some insects might be able to adapt, consuming a wide range of hydrocarbon base polymers. In this work, the adaptive capacity of Zophobas morio larvae when feeding on different synthetic polymers derived from petroleum was studied. Four different thirty-day larval feeding treatments were carried out with synthetic polymers, including expanded polystyrene (PS), low-density polyethylene (LDPE), polyisoprene (PI), and butyl rubber (BR); in addition, a positive control of organic diet was included. Intestinal bacteria were isolated from the treatments and identified by Sanger sequencing. To analyze the chemical composition and physical form of the frass produced, Fourier transform infrared spectroscopy (FITR) was performed, and images of the feces’ surfaces were taken with scanning electron microscopy (SEM), respectively. Zophobas morio larvae were able to consume 54% of PS in 30 days, equivalent to 3.2 mg/d/larva. Nine culturable bacterial strains associated with the decomposition of synthetic polymers were identified in the intestine of the larvae. As for the physicochemical analysis of the feces, FTIR spectra showed the scission of bands corresponding to functional groups of the synthetic polymers in the comparison of the plastic diet treatments versus the feces of antibiotic-treated and plastic-fed larvae, while the comparison of spectra of the plastic and control treatments also identified differences in the absorption peaks. SEM imaging demonstrated that superworm feces differed in dependence on the substrate consumed. The findings demonstrated that Zophobas morio larvae possess a gut biological complex that allows them to feed and survive by consuming various petroleum-derived polymers.

The Role of Household Social Support and Undermining in Dietary Change.

US adults find it challenging to meet disease prevention dietary recommendations and may participate in interventions to improve dietary quality. Social influences outside of the intervention, including level of social support and undermining of healthy eating in the home, may affect an individual's dietary intake. This secondary analysis examined (1) changes in household social support and undermining of healthy eating across a dietary intervention with household member participation and (2) the relationship between changes in social influences and dietary intake. Adults (N = 62) with low adherence to cancer prevention dietary recommendations recruited from the Philadelphia area participated in a 20-week dietary intervention focused on psychoeducation about NCI dietary recommendations and skills for behavior change. Half of the participants were also randomized to have an adult household member participate in some intervention contacts with them. Participants completed measures of social support and undermining of healthy eating and dietary intake at baseline and post-treatment (20weeks). Fifty-two participants had available data for baseline and post-treatment (i.e., completers). Household social support of healthy eating increased more among participants randomized to have household involvement in the intervention with a medium effect (η2 = .11). Fruit and vegetable intake significantly increased among participants with meaningful increases in household social support with a large effect (η2 = .37). There were no significant interaction effects of change in household undermining and time on change in dietary intake. Dietary interventions with a household support component show promise for improving household social support and may impact magnitude of dietary change.

Reinventing gut health: leveraging dietary bioactive compounds for the prevention and treatment of diseases

The human gut harbors a complex and diverse microbiota essential for maintaining health. Diet is the most significant modifiable factor influencing gut microbiota composition and function, particularly through bioactive compounds like polyphenols, dietary fibers, and carotenoids found in vegetables, fruits, seafood, coffee, and green tea. These compounds regulate the gut microbiota by promoting beneficial bacteria and suppressing harmful ones, leading to the production of key microbiota-derived metabolites such as short-chain fatty acids, bile acid derivatives, and tryptophan metabolites. These metabolites are crucial for gut homeostasis, influencing gut barrier function, immune responses, energy metabolism, anti-inflammatory processes, lipid digestion, and modulation of gut inflammation. This review outlines the regulatory impact of typical bioactive compounds on the gut microbiota and explores the connection between specific microbiota-derived metabolites and overall health. We discuss how dietary interventions can affect disease development and progression through mechanisms involving these metabolites. We examine the roles of bioactive compounds and their metabolites in the prevention and treatment of diseases including inflammatory bowel disease, colorectal cancer, cardiovascular diseases, obesity, and type 2 diabetes mellitus. This study provides new insights into disease prevention and underscores the potential of dietary modulation of the gut microbiota as a strategy for improving health.

Adapting a Telehealth Physical Activity and Diet Intervention to a Co-Designed Website for Self-Management After Stroke: Tutorial

People who experience a stroke are at a higher risk of recurrent stroke when compared with people who have not had a stroke. Addressing modifiable risk factors like physical inactivity and poor diet has been shown to improve blood pressure, a leading contributor to stroke. However, survivors of stroke often experience challenges with accessing risk reduction services including long wait lists, difficulty with transportation, fatigue, impaired function, and diminished exercise capacity. Providing health interventions via a website can extend the reach when compared with programs that are only offered face to face or via real-time telehealth. Given global challenges of accessing secondary prevention programs, it is important to consider alternative ways that this information can be made available to survivors of stroke worldwide. Using the “design thinking” framework and drawing on principles of the integrated knowledge translation approach, we adapted 2 co-designed telehealth programs called i-REBOUND – Let’s get moving (physical activity intervention) and i-REBOUND – Eat for health (diet Intervention) to create the i-REBOUND after stroke website. The aim of this paper is to describe the systematic process undertaken to adapt resources from the telehealth delivered i-REBOUND – Let’s get moving and i-REBOUND – Eat for health programs to a website prototype with a focus on navigation requirements and accessibility for survivors of stroke. We engaged a variety of key stakeholders with diverse skills and expertise in areas of stroke recovery, research, and digital health. We established a governance structure, formed a consumer advisory group, appointed a diverse project team, and agreed on scope of the project. Our process of adaptation had the following 3 phases: (1) understand, (2) explore, (3) materialize. Our approach considered the survivor of stroke at the center of all decisions, which helped establish guiding principles related to our prototype design. Careful and iterative engagement with survivors of stroke together with the application of design thinking principles allowed us to establish the functional requirements for our website prototype. Through user testing, we were able to confirm the technical requirements needed to build an accessible and easy-to-navigate website catering to the unique needs of survivors of stroke. We describe the process of adapting existing content and co-creating new digital content in partnership with, and featuring, people who have lived experience of stroke. In this paper, we provide a road map for the steps taken to adapt resources from 2 telehealth-delivered programs to a website format that meets specific navigation and accessibility needs of survivors of stroke.

Mechanisms of Action of Exclusive Enteral Nutrition and Other Nutritional Therapies in Crohn’s Disease

Background and Objectives: Crohn’s disease (CD) is an inflammatory bowel disease (IBD) characterized by transmural inflammation and intestinal fibrosis involving mostly the small intestine and colon. The pathogenic mechanisms of CD remain incompletely understood and cures are unavailable. Current medical therapies are aimed at inducing prolonged remission. Most of the medical therapies such as corticosteroids have substantial adverse effects. Consequently, many dietary therapies have been explored for the management of CD. Up to now, exclusive enteral nutrition (EEN) has been considered the only established dietary treatment for IBD, especially CD. In this article, we aim to give a concise review about the current therapeutic options and challenges in the management of CD and aim to compare the efficacy of EEN with other dietary therapies and update on the possible mechanisms of the benefits of EEN and other nutritional therapies. Methods: We searched the literature up to August 2024 through PubMed, Web of Science, and other sources using search terms such as EEN, nutritional therapy, IBD, Crohn’s disease, ulcerative colitis. Clinical studies in patients and preclinical studies in rodent models of IBD were included in the summary of the therapeutic benefits. Results and Conclusions: EEN involves oral or nasogastric tube feeding of a complete liquid diet with exclusion of normal foods for a defined period (usually 6 to 8 weeks). EEN treatment is demonstrated to have anti-inflammatory and healing effects in CD through various potential pathways, including altering gut bacteria and their metabolites, restoring the barrier function, direct anti-inflammatory action, and indirect anti-inflammatory action by eliminating mechanical stress in the bowel. However, efficacy of other nutritional therapies is not well established in CD, and mechanisms of action are largely unknown.

Trace mineral source influences in vitro fermentation characteristics and trace mineral solubility.

Two experiments were conducted to determine: 1) the impact of strained rumen fluid (SRF) alone or SRF with particle-associated microorganisms (PAO) included and dilution on in vitro dry matter digestibility (DMD) and 2) the impact of trace mineral (TM) source on in vitro fermentation characteristics and TM solubility under simulated abomasal and intestinal conditions. In Experiment 1, three cannulated steers were adapted to a diet formulated to meet the nutrient requirements for lactating dairy cows. Strained RF was obtained by straining rumen content through 2 layers of cheesecloth. Half of the remaining digesta was washed with McDougall's buffer and filtered through 2 layers of cheesecloth to obtain PAO. Both SRF and PAO were filtered again through 8 layers of cheesecloth. Strained RF was mixed with either McDougall's buffer (SRF) or PAO (SRF+PAO) at a ratio of 1:2 or 1:4 and incubated at 39°C for 12h using the ground basal diet as the substrate. Digestibility of DM was greater in digestion tubes containing SRF and SRF+PAO at a 1:2 ratio. In Experiment 2, eight steers fitted with ruminal cannula were blocked by body weight and assigned to one of two treatment groups. Treatments consisted of 10mg Cu, 40mg Mn, and 60mg Zn/kg DM from either: 1) sulfate (STM) or 2) hydroxychloride (HTM) sources. Steers were housed in individual pens and fed the same diet as described in experiment 1. Dietary TM treatments were mixed with dried distillers grains and mixed in the diet, by hand, immediately after basal diet delivery. Dietary treatments were fed for 14 d. On day 15, SRF+PAO was collected from each steer (STM-RF and HTM-RF) and used in a series of in vitro crossover experiments. In vitro substrates (S) used were the ground diets consumed by the animals on each treatment (STM-S and HTM-S). Incubations containing HTM-S had greater (P < 0.01) total VFA concentration and propionic acid molar proportions, but lesser (P < 0.01) acetic acid molar proportions than STM-S. Rumen fluid from steers supplemented with HTM had a greater (P < 0.03) total VFA than STM-RF at 24h post incubation. After 12h post incubation, the molar proportion of propionic acid in HTM-RF was lesser (P = 0.04) than STM-RF. After simulated abomasal digestion, soluble Mn concentration in HTM-S was greater (P < 0.01) than STM-S. These data indicate that the source of trace minerals can influence in vitro rumen fermentation characteristics and Mn solubility under simulated abomasal conditions.

Effect of Diet and Dietary Patterns on the Progression of Multiple Sclerosis: A Review.

The link between diet and the progression of Multiple Sclerosis (MS) is a topic of growing interest and investigation within the medical community. This review explored the mechanisms through which dietary interventions can impact the course of MS and shape the clinical outcomes and quality of life of individuals with the disease. By synthesizing current knowledge from clinical studies and observational research, the review aimed to provide in-sights into the role of diet in managing MS. A comprehensive literature search was conducted, focusing on the effect of diet and dietary patterns on the progression of MS. Key findings indicated that individuals with higher diet quality exhibit reduced disability levels and lower symptom severity, emphasizing the importance of maintaining a healthy diet and adopting a holistic, healthy lifestyle in managing MS. The review also delved into the potential impact of macronutrients, vitamins, and minerals on the progression of MS, highlighting the im-portance of adequate nutrient intake for optimal health outcomes. Additionally, the study explored the association between dietary intake variations and the severity of MS, suggesting that further investigation is needed to understand the potential implications of nutrient defi-ciencies in MS patients. Overall, the review serves as a valuable resource for healthcare pro-fessionals and individuals living with MS, providing evidence-based dietary approaches that may help optimize health outcomes and mitigate the burden of the disease. It also calls for future research directions in the critical area of dietary management of MS to enhance our understanding and improve patient care.

Effects of Daily Almond Consumption on Glycemia in Adults with Elevated Risk for Diabetes: A Randomized Controlled Trial.

The purpose of this study was to examine the potential for sustained almond consumption to reduce HbA1c concentrations among individuals with elevated values. A 16-week randomized, parallel-arm, controlled trial was conducted. Eighty-one adults with elevated HbA1c concentrations (>5.7%) were randomly assigned to incorporate 2 oz of raw almonds (A: n=39) or energy-matched snacks (C: n=42) into their daily diets. Body weight, body composition, plasma lipids, HbA1c, plasma vitamin E, glycemia (by meal tolerance test and continuous glucose monitoring), dietary intake, and hedonic responses to test foods were measured at stipulated time points. Participants consuming almonds ingested 253 kcal/d more than participants in the control group (P=0.02), but this did not result in a significant difference in body weight. No statistically significant differences were observed in HbA1c concentrations, blood chemistries, body composition, or glycemia over time or between groups. However, Healthy Eating Index scores improved within the almond group as compared to the control group (P<0.001). Additionally, the hedonic rating of almonds within the almond group did not decline as markedly as the control group's reduced liking of the pretzel snack. Alpha-tocopherol increased significantly, and gamma tocopherol tended to decrease in the almond group, indicating compliance with the dietary intervention. Overall, daily ingestion of 2oz of raw almonds in a self-selected diet for 16 weeks did not alter short-term or longer-term glycemia or HbA1c concentrations in adults with elevated HbA1c concentrations, but they were well-tolerated hedonically and improved diet quality without promoting weight gain.