Amount Of Diet Research Articles

The impacts of diet on cardiac performance under changing environments.

Natural and anthropogenic stressors are dramatically altering environments, impacting key animal physiological traits, including cardiac performance. Animals require energy and nutrients from their diet to support cardiac performance and plasticity; however, the nutritional landscape is changing in response to environmental perturbations. Diet quantity, quality and options vary in space and time across heterogeneous environments, over the lifetime of an organism and in response to environmental stressors. Variation in dietary energy and nutrients (e.g. lipids, amino acids, vitamins, minerals) impact the heart's structure and performance, and thus whole-animal resilience to environmental change. Notably, many animals can alter their diet in response to environmental cues, depending on the context. Yet, most studies feed animals ad libitum using a fixed diet, thus underestimating the role of food in impacting cardiac performance and resilience. By applying an ecological lens to the study of cardiac plasticity, this Commentary aims to further our understanding of cardiac function in the context of environmental change.

Energy Intake and Dietary Glycemic Load in Late Morning and Risk of Type 2 Diabetes: The Hispanic Community Health Study/Study of Latinos-A Multicenter Prospective Cohort Study.

To evaluate the association between meal timing and type 2 diabetes risk in U.S. Hispanic/Latino adults. The Hispanic Community Health Study/Study of Latinos (HCHS/SOL) is a multicenter, community-based, prospective cohort study. This study included 8,868 HCHS/SOL adults without diabetes at baseline (2008-2011) and attending the visit 2 examination (2014-2017). Energy intake and glycemic load (GL) in each meal timing were assessed at baseline using two 24-h dietary recalls. Incident diabetes was identified through annual follow-up calls or at visit 2. Hazard ratios (HRs) for incident diabetes were estimated using Cox models, accounting for the complex survey design. The study population (50.9% female) had a baseline mean age of 39.0 (95% CI, 38.4-39.5) years. Over a median (range) follow-up of 5.8 (0.8-9.6) years, 1,262 incident diabetes cases were documented. Greater energy intake and GL in late morning (9:00-11:59 a.m.) were associated with a lower diabetes risk, whereas greater energy intake and GL in other meal timings were not. After accounting for diet quantity and quality, sociodemographic characteristics, lifestyle factors, and chronic conditions, the HRs were 0.94 (95% CI, 0.91-0.97) per 100-kcal energy intake increment and 0.93 (0.89-0.97) per 10-unit GL increment in late morning. Replacing energy intake or GL from early morning (6:00-8:59 a.m.), afternoon (12:00-5:59 p.m.), or evening (6:00-11:59 p.m.) with late-morning equivalents was associated with a comparably lower diabetes risk. This study identified late morning as a favorable meal timing in Hispanic/Latino adults, providing a novel perspective on type 2 diabetes prevention that warrants confirmation.

Very-low-calorie diet-based intensive lifestyle intervention for remission of type 2 diabetes: Real-world experience in a South Asian population.

Very low-calorie diet (VLCD) can induce weight loss and diabetes remission (DR) amongst people with obesity and recent-onset type 2 diabetes (T2D). We aimed to determine the effectiveness and acceptability of VLCD in achieving DR amongst Sri Lankan adults with T2D. A retrospective analysis was conducted in a diabetes practice where VLCD-based Diabetes Remission Programme (VDRP) was offered for adults (>18 years) with T2D for <3 years and body mass index over 25 kg/m2. VLCD (~800 kcal/day, provided with/without diet replacement formula) was offered for 8-12 weeks, followed by gradual food reintroduction and exercise. DR was defined as HbA1c <6.5% at least 3 months after stopping glucose-lowering medications. A total of 170 participants who enrolled in the VDRP (mean age 38.4 years [±11.1], men 68%, mean baseline HbA1c 86.9 [±18.1] mmol/mol (10.1 [±2.1]%), median duration of T2D 2 years [IQR 1-2]) and 87 (51%) of them followed the programme (attended at least one follow-up visit). Amongst the individuals who followed the VDRP, 40.2% achieved DR (35/87), compared with 2.4% (2/83) amongst those who did not follow the VDRP (aHR 9.3, 95% CI 2.2-16.4, p = 0.002). The proportion achieving normoglycaemia (HbA1c < 6.5%) but continued to take glucose-lowering medication was 20/87 among VDRP followers and 20/85 amongst VDRP non-followers. The commonest reasons for not following the VDRP were too restrictive dietary quantity (92%) and difficulties in finding recommended food items (67%). Majority (79%) would recommend VDRP to others. VDRP is effective in achieving T2D remission amongst Sri Lankan adults with recently diagnosed T2D and obesity. Over half of the participants followed the programme and over 75% would recommend it to others, indicating good acceptability.

Tea (Camellia sinensis) leaves accumulate higher levels of Aluminum: Potential Health Risk- Alzheimer’s disease (AD): An updated review of evidence

This review paper of literature highlights the role of accumulation of aluminum in tea leaves and its toxic effects on human health. Tea is the most consumed beverage in the world after water and contains heavy metals and trace elements that may cause potential negative effects on health. Tea is known to be a metal accumulator. However, drinking even a very high dietary amount of black tea or green tea would be unlikely to cause these adverse effects in humans. Thus, for the majority of heavy tea drinkers around the world, tea is likely to be the largest single source of aluminum intake. Addition of milk to tea can decrease or completely inhibit tea antioxidant properties. According to the literature survey, it is well known that the tea plant accumulates aluminum in its leaves to a greater extent than most other edible plants, and would be a potentially important source of dietary aluminum. Aluminum can be transferred into tea infusions through brewing tea, then enter the human body via tea drinking, thereby causing potential harm to human health. On the basis of literature survey, even though some epidemiological reports were contradictory, there was mounting scientific evidence suggesting a relationship between the neurotoxicity of aluminum and the pathogenesis of Alzheimer’s disease. In particular, the link between aluminum and Alzheimer’s disease has been the subject of scientific debate for several decades. The literature survey confirmed that aluminum hypothesis has been the subject of much debate and criticism for several decades. Evidence from human clinical trials suggested that moderate (1–6 cups/day) rather than excessive consumption of tea could bring diverse health benefits. The relationship between tea consumption and dementia has not been well established yet and other studies reported no significant association. The cause of Alzheimer's disease and any association with aluminum is still unknown. There have been conflicting findings in the scientific literature.

Phenotypically plastic responses to environmental variation are more complex than life history theory predicts.

For insects that exhibit wing polyphenic development, abiotic and biotic signals dictate the adult wing morphology of the insect in an adaptive manner such that in stressful environments the formation of a flight-capable morph is favored and in low-stress environments, a flightless morph is favored. While there is a relatively large amount known about the environmental cues that dictate morph formation in wing polyphenic hemipterans like planthoppers and aphids, whether those cues dictate the same morphs in non-hemipteran (i.e., cricket) wing polyphenic species has not been explicitly investigated. To experimentally test the generality of environmental cue determination of wing polyphenism across taxa with diverse life histories, in this study, we tested the importance of food quantity, parasitic infection, and tactile cues on wing morph determination in the wing polyphenic sand field cricket, Gryllus firmus. Our results also show that certain stress cues, such as severe diet quantity limitation and parasitic infection, actually led to an increase in the production of flightless morph. Based on these findings, our results suggest that physiological and genetic constraints are important to an organism's ability to respond to environmental variation in an adaptive manner beyond simple life history trade-offs.

Are first-trimester pregnant women consuming adequate and diverse diet? A hospital-based cross-sectional study in Karachi, Pakistan

BackgroundMaintaining a healthy and diverse diet during pregnancy is crucial for maternal well-being and fetal development. The first trimester marks the beginning of vital developmental processes influenced by maternal nutritional status. Therefore, we aimed to determine dietary adequacy and diversity among first-trimester pregnant women.MethodsIn this cross-sectional study, we recruited 306 first-trimester pregnant women from the antenatal clinics of Aga Khan University Hospital, Karachi (January 2020 to September 2021). Eligible women possessed smartphones (for the mHealth intervention trial) and reported no major comorbidities or medication use. Data about socio-demographic, obstetric, and dietary history were collected through interviews using a structured questionnaire. Booking weight, height, blood pressure, and haemoglobin levels were extracted from medical records. An aggregate dietary risk score (DRS) was calculated separately for quantity and quality by summing the DRS for each of the six major food groups. A score of 0 was assigned to adequate, 1.5 to intermediate, and 3 to inadequate quantity or quality categories. Data were analysed using STATA 14.0.ResultsThe mean ± SD for DRS quantity and quality were 10.6 ± 2.4 and 7.5 ± 2.5, respectively. Adequate dietary quantity and quality per week for starch-based food were reported by 14.4% and 21.2%, for vegetables by 0.3% and 49%, for fruits by 41.2% and 88.6%, for animal and plant protein by 19% and 0%, for milk and milk products by 1% and 37.6% and for oils and fats by 90.5% and 8.8%, respectively. Sweet and savoury snacks were eaten by 74.8% and 53.9%, respectively. Ready-made meals, carbonated beverages, packaged juices, and additional salt were consumed by 55.2%, 46.4%, 34.3%, and 7.5%, respectively. The median (IQR) water intake was 6 (4–8) glasses/day.ConclusionsDuring the early stages of pregnancy, women enrolled for antenatal care at an urban private tertiary care hospital report inadequate dietary intake for various food groups, except for the quantity of oils/fats and the quality of fruit consumption. Poor dietary practices underscore the need for focused and impactful dietary counselling during the initial stages of pregnancy.

Pro-inflammatory diets are associated with higher C-reactive protein and lower plasma concentrations of vitamins with anti-inflammatory potential, in the EPIC-Norfolk cohort

The development of multiple long-term conditions (MLTC) has been shown to be associated with low-grade chronic inflammation(1). The Dietary Inflammatory Index (DII®) is a literature-based dietary score that was developed to measure the potential impact of diet on the inflammatory status of an individual(2). In this study, we aimed to validate the DII® score against biomarkers, including high- sensitivity C-reactive protein (hs-CRP), and plasma concentrations of vitamin C, retinol and α- tocopherol in European Prospective Investigation Into Cancer and Nutrition (EPIC)-Norfolk participants, aged 39–79 years at baseline(3).The DII® score was calculated using a 130-item Food Frequency Questionnaire collected at baseline, between 1993 and 1997. The dietary intakes were adjusted to a 2000 kcal/day diet, to assess diet quality independently of diet quantity. Non-fasting serum cholesterol, hs-CRP, and plasma α- tocopherol, vitamin C and retinol concentrations were also measured at this time-point. Data collected via a self-administered Health and Lifestyle Questionnaire were used to establish classification of a number of variables. Analyses were conducted on sub-samples with a DII® score and measures of hs-CRP (8,034 men and 9,861 women), and concentrations of vitamin C (9,866 men and 11,702 women), retinol (3,673 men and 3,517 women) and cholesterol-adjusted α- tocopherol (3,623 men and 3,476 women). Analysis of covariance and linear regression were used to study associations across sex-specific quintiles of the DII® score (adjusted for age, BMI, smoking status, physical activity, social class and educational level), where a higher score indicates a more pro-inflammatory diet.Mean concentrations in men and women were 2.99 and 3.09 mg/L for hs-crp, 47 and 59 μmol/L for vitamin C, 53 and 50 μg/dL for retinol and 4.34 and 4.42 μmol/mmol for cholesterol-adjusted α- tocopherol, respectively. In both men and women, mean hs-CRP was higher if the diet was more pro-inflammatory (p-trend = 0.02 in men and 0.07 in women), while concentrations of vitamin C, retinol and α-tocopherol were significantly lower (p-trend &lt; 0.001). Positive associations for hs-CRP, but negative associations for plasma concentrations of vitamin C, retinol and α-tocopherol were evident in both men and women, after adjustments for covariates (p-trend &lt; 0.001). The differences between Q1 and Q5 adjusted means for hs-CRP, vitamin C, retinol and α-tocopherol were +9.4%, -22.1%, -3.9% and -8.6% in men and +7.9%, -17.5%, -4.8% and -7.6% in women, respectively.We observed statistically significant positive associations between the DII® score and hs-CRP, a well-known inflammatory biomarker, whilst significant negative associations were found for circulating concentrations of three anti-inflammatory vitamins, after adjustment for covariates. These findings indicate that the DII® score is a valid measure of the inflammatory potential of diet in these middle-aged and old adults, making it possible to study the inflammatory role of diet in MLTC development.

Influence of adult body size on blood feeding behavior and eggs retention in Aedes albopictus (Diptera: Culicidae).

Mosquito-borne viruses continue to affect billions of people globally, posing a severe health risk and an economic burden. Aedes albopictus (Skuse), a highly invasive mosquito species, has repeatedly invaded and increased its presence, serving as a key vector of dengue virus, yellow fever virus (YFV), Zika virus (ZIKV), and Chikungunya virus (CHIKV), causing frequent outbreaks of related viral diseases. This study investigated the impact of larval diet quantity on larval duration and adult body size. The effect of adult mosquito body size on various aspects of Ae. albopictus was also examined, including blood-feeding behavior, follicular development, reproductive capacity, egg retention capacity, preoviposition period, and fecundity. These diverse characteristics all have an effect on arboviruses transmission. The changes in body size (small, medium, and large) are obtained by providing different quantities of larval diet (low, average, and high). The results indicate that the quantity of larval diet directly impacts the adult body size while inversely affecting the larval duration. Furthermore, a positive correlation exists between adult body size and wing length, implying that wing length could be a reliable indicator of adult body size and rearing conditions during the developmental stages. Large females exhibited higher numbers of follicles and greater fecundity. Moreover, a significant correlation was observed between follicle number before the first blood meal and total egg number. In contrast, increasing wing length decreased the number of blood meals, egg retention, and the preoviposition period. The tendency of small females to perform multiple feedings was greater than that of large females. Small females exhibited a higher propensity for multiple feeding activities when compared to their larger counterparts. Most medium-sized females (92.8%) deposited eggs in their ovaries, however, 7.2% retained a few. In contrast, most large females (87.4%) had complete ovary egg-laying, whereas a minority (12.6%) retained some of their eggs. About 35.2% of small females showed ovarian egg retention, while 64.8% successfully laid all their eggs. After the first blood meal, the oviposition rate was 92% for large females, 88% for medium females, and 76% for small females. About 69.86% of the follicles in large females underwent vitellogenesis. This finding suggests that small females with low energy reserves exhibited incomplete oviposition and multiple blood feedings to increase their reproductive capacity. RESEARCH HIGHLIGHTS: Add more information about arbovirus epidemics and their consequences. Aedes albopictus is a global invasive species that transmit dengue virus, CHIKV, YFV, and ZIKV. A negative correlation was observed between body size, egg retention, and multiple blood feedings in Aedes albopictus. Size of the female's body was positively correlated with fecundity, while it was negatively correlated with the preoviposition period. Size-dependent multiple blood feeding affects vector-host contact frequency.

Cel-CS1K: A Celastrol-Chitosan Conjugate for Treating Diet-Induced Obesity.

Celastrol (Cel), extracted from Tripterygium wilfordii Hook, is a potential antiobesity drug, except for its adverse reactions in clinic. In the present study, we synthesized a promising celastrol-chitosan conjugate (Cel-CS1K) and evaluated its antiobesity effect and biological safety in diet-induced obese mice. Cel-CS1K showed higher drug loading (over 10 wt %), good solubility (18-19 mg/mL) in water, slower peak time (Tmax = 4 h), and clearance (T1/2 = 8.97 h) in rats. Cel-CS1K effectively attenuated the cytotoxicity, celastrol-induced apoptosis, and fat accumulation of hepatocytes. Cel-CS1K reduced body weight and dietary amount same as the free Cel but with lower toxicity in blood, liver, and testis. Cel-CS1K improved the glucose homeostasis, HDL-C level, insulin sensitivity, and leptin sensitivity, while it significantly reduced the gene expression levels of LDL-C, TG, and TC in obese mice. Furthermore, the adipose-related gene expression levels provided evidence in support of a role for Cel-CS1K in losing weight by the multimode regulation. Overall, Cel-CS1K provides a translatable therapeutic strategy for the treatment of diet-induced obese humans.

Eating behaviors and dietary intake 2 to 4 years following sleeve gastrectomy: Are there any associations?

ObjectivesA lack of research exists on associations between eating behaviors and dietary intake among patients undergoing bariatric surgery. This study aimed to ascertain the association between eating behaviors and dietary quantity and quality at mid-term postsleeve gastrectomy (SG). MethodsThis cross-sectional study included 146 adults within 2 to 4 years post-SG. Dietary intake and eating behaviors were assessed using a food frequency questionnaire and the Dutch Eating Behavior Questionnaire, respectively. Energy and the proportion of macronutrients were determined. Carbohydrate quality index (CQI), fat quality index (FQI), healthy plate protein quality index (HPPQI), macronutrient quality index (MQI), and Healthy Eating Index (HEI)-2015 were computed to evaluate diet quality. Participants were categorized into high and low groups based on median scores for each eating behavior scale. Least-squared (geometric) means and 95% confidence intervals were determined for each group using the analysis of covariance, adjusted for age and sex. ResultsThe mean time since surgery for participants was 30.8 ± 6.5 months, and 77.4% were female. Participants with high emotional eating had higher energy and HPPQI. Individuals with high external eating had higher energy and percentages of fat and lower FQI and HEI scores. Individuals with high restrained eating scores demonstrated lower energy intake but a higher percentage of protein intake and higher CQI, FQI, MQI, and HEI scores. ConclusionsExternal eating showed the most unfavorable associations, while restrained eating showed the most favorable associations with quantity and quality of diet 2 to 4 years after SG. Emotional eating was minimally related to dietary characteristics.

Effect of quality and quantity of diet on nutritional status of under-five children residing in the rural area: A longitudinal study.

Variety and adequacy of intake of food are required to meet the nutritional needs of the children. Due to poverty/illiteracy, poor families depend on a single food group that is, cereals. Due to the pandemic also, the nutrition of the growing children is affected. To know the effect of quantity and quality of diet on the nutritional status of under-five children. This study had 270 children aged between 1 and 4 years registered at Anganwadi as study participants. Information on sociodemographic variables, quantity of calories and proteins consumed, quality of diet, and anthropometry of children was collected. Percentages and paired t-tests were used to find the difference between nutritional status and diet at different intervals, which was found to be statistically significant (P < 0.05). In our study, we found that as the quantity and quality of diet improved nutritional status, especially weight for age, body mass index (BMI) for age Z score also improved. There was a significant difference between calories consumed at the baseline, 6 months and 1 year with P < 0.05. Protein intake was significant between baseline and six months with P < 0.005. Our study found a high percentage of undernutrition and wasting in the under-five population when diet was not sufficient in quality and quantity. As the quality and quantity of diet improved nutritional status also improved.

The Effect of an Elevated Dietary Copper Level on the Vascular Contractility and Oxidative Stress in Middle-Aged Rats.

Copper (Cu), being an essential mineral, plays a crucial role in maintaining physiological homeostasis across multiple bodily systems, notably the cardiovascular system. However, an increased Cu level in the body may cause blood vessel dysfunction and oxidative stress, which is unfavorable for the cardiovascular system. Middle-aged (7-8 months old) male Wistar rats (n/group = 12) received a diet supplemented with 6.45 mg Cu/kg (100% of the recommended daily dietary quantity of copper) for 8 weeks (Group A). The experimental group received 12.9 mg Cu/kg of diet (200%-Group B). An ex vivo study revealed that supplementation with 200% Cu decreased the contraction of isolated aortic rings to noradrenaline (0.7-fold) through FP receptor modulation. Vasodilation to sodium nitroprusside (1.10-fold) and acetylcholine (1.13-fold) was potentiated due to the increased net effect of prostacyclin derived from cyclooxygenase-1. Nitric oxide (NO, 2.08-fold), superoxide anion (O2•-, 1.5-fold), and hydrogen peroxide (H2O2, 2.33-fold) measured in the aortic rings increased. Blood serum antioxidant status (TAS, 1.6-fold), Cu (1.2-fold), Zn (1.1-fold), and the Cu/Zn ratio (1.4-fold) increased. An increase in Cu (1.12-fold) and the Cu/Zn ratio (1.09-fold) was also seen in the rats' livers. Meanwhile, cyclooxygenase-1 (0.7-fold), cyclooxygenase-2 (0.4-fold) and glyceraldehyde 3-phosphate dehydrogenase (0.5-fold) decreased. Moreover, a negative correlation between Cu and Zn was found (r = -0.80) in rat serum. Supplementation with 200% Cu did not modify the isolated heart functioning. No significant difference was found in the body weight, fat/lean body ratio, and organ weight for either the heart or liver, spleen, kidney, and brain. Neither Fe nor Se, the Cu/Se ratio, the Se/Zn ratio (in serum and liver), heme oxygenase-1 (HO-1), endothelial nitric oxide synthase (eNOS), or intercellular adhesion molecule-1 (iCAM-1) (in serum) were modified. Supplementation with 200% of Cu potentiated pro-oxidant status and modified vascular contractility in middle-aged rats.

Abstract 31: Energy Intake and Dietary Glycemic Load in Late Morning and Risk of Type 2 Diabetes: The Hispanic Community Health Study/Study of Latinos (HCHS/SOL)

Introduction: Meal timing may affect type 2 diabetes risk due to the circadian regulation of glucose metabolism. However, the optimal meal timing for lowering diabetes risk remains unknown. Objective and Hypothesis: We aimed to identify the optimal meal timing for diabetes prevention. Since glucose tolerance peaks in the morning, we hypothesized that eating more in the morning is associated with a lower risk of diabetes when daily diet quantity and quality are held constant. Methods: We included 7716 participants from the Hispanic Community Health Study/Study of Latinos (HCHS/SOL) who were free of diabetes at baseline (2008-2011) and attended clinic visit 2 (2014-2017). Incident diabetes was identified during the annual phone follow-up (through self-reported diabetes) or at visit 2 (through self-reported diabetes or antihyperglycemic medication usage or laboratory tests using the ADA criteria). Dietary intakes at baseline were assessed using two 24-hour dietary recalls, and meal timing was categorized into early morning (6:00-8:59 AM), late morning (9:00-11:59 AM), afternoon (12:00-5:59 PM), evening (6:00-11:59 PM), and night (0:00-5:59 AM). We calculated energy intake (EI) in kilocalories and energy-adjusted glycemic load (GL) in each meal timing. We estimated their associations with incident diabetes using the Cox proportional hazards models, accounted for the HCHS/SOL complex survey design and adjusted for age, sex, field center, Hispanic/Latino background, income, education, smoking, alcohol intake, sleep duration, physical activity, BMI, family history of diabetes, hypertension, dyslipidemia, total energy intake, and diet quality (Health Eating Index for energy intake in each meal timing as the exposure; total GL and fiber intake for GL in each meal timing as the exposure). Results: At baseline, the analytic population had a mean (SE) age of 39.0 (0.3) years, and 50.9% were female. Over a median (range) follow-up of 5.8 (0.8-9.6) years, 1,262 incident diabetes cases were documented. EI and GL exhibited strong positive correlations throughout the day. EI and GL in late morning were inversely associated with diabetes risk, whereas those in other meal timings were not associated with diabetes risk. In fully adjusted models, the hazard ratios (HRs) of diabetes were 0.94 (95% CI: 0.91 to 0.97) for a 100-kcal increase of EI and 0.93 (0.89 to 0.97) for a 10-unit increase of GL in late morning. The associations were stronger and showed a linear dose-response relationship in males and adults with overweight/obesity. Replacing energy intake or GL, from early morning, afternoon, or evening with late morning equivalents was associated with comparable reductions in diabetes risk. Conclusions: This study identifies late morning as a favorable meal timing for lowering diabetes risk in Hispanic/Latino adults, providing a novel perspective on type 2 diabetes prevention that warrants confirmation.

Association between dietary phosphate intake and skeletal muscle energetics in adults without cardiovascular disease.

Highly bioavailable inorganic phosphate (Pi) is present in large quantities in the typical Western diet and represents a large fraction of total phosphate intake. Dietary Pi excess induces exercise intolerance and skeletal muscle mitochondrial dysfunction in normal mice. However, the relevance of this to humans remains unknown. The study was conducted on 13 individuals without a history of cardiopulmonary disease (46% female, 15% Black participants) enrolled in the pilot-phase of the Dallas Heart and Mind Study. Total dietary phosphate was estimated from 24-h dietary recall (ASA24). Muscle ATP synthesis was measured at rest, and phosphocreatinine (PCr) dynamics was measured during plantar flexion exercise using 7-T 31P magnetic resonance (MR) spectroscopy in the calf muscle. Correlation was assessed between dietary phosphate intake normalized to total caloric intake, resting ATP synthesis, and PCr depletion during exercise. Higher dietary phosphate intake was associated with lower resting ATP synthesis (r = -0.62, P = 0.03), and with higher levels of PCr depletion during plantar flexion exercise relative to the resting period (r = -0.72; P = 0.004). These associations remain significant after adjustment for age and estimated glomerular filtration rate (both P < 0.05). High dietary phosphate intake was also associated with lower serum Klotho levels, and Klotho levels are in turn associated with PCr depletion and higher ADP accumulation post exercise. Our study suggests that higher dietary phosphate is associated with reduced skeletal muscle mitochondrial function at rest and exercise in humans providing new insight into potential mechanisms linking the Western diet to impaired energy metabolism.NEW & NOTEWORTHY This is the first translational research study directly demonstrating the adverse effects of dietary phosphate on muscle energy metabolism in humans. Importantly, our data show that dietary phosphate is associated with impaired muscle ATP synthesis at rest and during exercise, independent of age and renal function. This is a new biologic paradigm with significant clinical dietary implications.

The association of dietary carbohydrate quality and quantity with obesity among Iranian adolescents: a case-control study

BackgroundAdolescent obesity is considered as a major health concern worldwide which is closely linked to the quality of diet. The purpose of the present study was to assess the carbohydrate quality and quantity in relation to odds of overweight and obesity in adolescents.MethodsThis case-control study with a 1:1 ratio was conducted on 406 adolescents (14 to 18 years old) matched for age and gender. Participants were selected by multistage cluster random sampling method from March to October 2019 in Shiraz, Iran. Dietary intakes of the study population were assessed by a validated semi-quantitative food frequency questionnaire. Also anthropometric indices were measured using standard methods and demographic information was recorded via face to face interview. The relation between low carbohydrate diet score (LCDS) and carbohydrate quality index (CQI), and odds of obesity was evaluated by multiple Logistic regression.ResultsAfter adjusting the role of potential confounders, the participants in the third tertiles of LCDS (OR = 0.443, 95% CI = (0.260 to 0.755)) and CQI (OR = 0.005, 95% CI = (0.001 to 0.025)) had less odds of being overweight and obese compared to the first tertile.ConclusionThe present study found an inverse relationship between dietary quantity and quality of carbohydrate intake and the odds of overweight and obesity in a sample of Iranian adolescents.

Independent and interactive effects of diet and entomopathogenic microsporidia on an outbreaking forest insect defoliator

Abstract Insect herbivore diet mediates interactions with entomopathogens, yet this is relatively unexplored for microsporidia. Here, we examine a diet‐mediated tri‐trophic interaction between an outbreaking forest defoliator, forest tent caterpillar (FTC) Malacosoma disstria Hübner and Nosema sp. microsporidia. We conducted two experiments where diet quality was manipulated by incorporating lyophilized aspen (Populus tremuloides Michaux) foliage into an artificial diet, which was compared to a standard artificial diet. Diet quantity varied between fully fed and partially starved conditions, simulating an outbreak scenario. Microsporidia infection occurred naturally or was induced via experimental inoculation. We assessed FTC survival, microsporidia infection and load, and sublethal effects of treatments on FTC traits. Plant secondary metabolite concentrations in aspen‐augmented diets varied between experiments. In Experiment 1, the aspen‐augmented diet contained lyophilized aspen foliage with low concentrations of secondary metabolites, which increased FTC survival and reduced microsporidia infection. Diet quality and infection load also interactively influenced adult wing traits in Experiment 1. In Experiment 2, the aspen‐augmented diet contained lyophilized aspen foliage with higher concentrations of secondary metabolites, which negatively affected FTC. No diet‐mediated interactions with microsporidia were observed in Experiment 2. Diet quality (Experiments 1 and 2), diet quantity (Experiment 2) and microsporidia infection (Experiments 1 and 2) directly influenced FTC survival and/or had sublethal effects on FTC that may have cascading effects on population dynamics and dispersal. We demonstrated that diet quality can mediate interactions between FTC and microsporidia, but these interactions depend on the defensive chemistry of the FTC diet. To our knowledge, this is the first study to report diet‐mediated interactions between an outbreaking forest pest and microsporidia and one of only a few studies to examine this tri‐trophic interaction among Lepidoptera.

Transcriptome analysis and immune gene expression of channel catfish (Ictalurus punctatus) fed diets with inclusion of frass from black soldier fly larvae.

The larval waste, exoskeleton shedding, and leftover feed components of the black soldier fly and its larvae make up the by-product known as frass. In this study, we subjected channel catfish (Ictalurus punctatus) to a 10-week feeding trial to assess how different dietary amounts of frass inclusion would affect both systemic and mucosal tissue gene expression, especially in regard to growth and immune-related genes. Fish were divided in quadruplicate aquaria, and five experimental diets comprising 0, 50, 100, 200, and 300g of frass per kilogram of feed were fed twice daily. At the end of the trial, liver, head kidney, gill, and intestine samples were collected for gene expression analyses. First, liver and intestine samples from fish fed with a no frass inclusion diet (control), low-frass (50g/kg) inclusion diet, or a high-frass (300g/kg) inclusion diet were subjected to Illumina RNA sequencing to determine global differential gene expression among diet groups. Differentially expressed genes (DEGs) included the upregulation of growth-related genes such as glucose-6-phosphatase and myostatin, as well as innate immune receptors and effector molecules such as toll-like receptor 5, apolipoprotein A1, C-type lectin, and lysozyme. Based on the initial screenings of low/high frass using RNA sequencing, a more thorough evaluation of immune gene expression of all tissues sampled, and all levels of frass inclusion, was further conducted. Using targeted quantitative PCR panels for both innate and adaptive immune genes from channel catfish, differential expression of genes was identified, which included innate receptors (TLR1, TLR5, TLR9, and TLR20A), proinflammatory cytokines (IL-1β type a, IL-1β type b, IL-17, IFN-γ, and TNFα), chemokines (CFC3 and CFD), and hepcidin in both systemic (liver and head kidney) and mucosal (gill and intestine) tissues. Overall, frass from black soldier fly larvae inclusion in formulated diets was found to alter global gene expression and activate innate and adaptive immunity in channel catfish, which has the potential to support disease resistance in this species in addition to demonstrated growth benefits.

Addressing Nutritional Challenges: Strategies for Improving Health Outcomes in India and Global Hunger Concerns

In India, the prevalence of unhealthy dietary patterns and nutritional deficiencies remains a significant public health concern, posing challenges to food, nutrition, and health security. This paper explores key interventions to improve women’s and children’s health and nutrition, including enhancing diet quantity and quality, preventing micronutrient deficiencies like anaemia, and increasing access to essential nutrition and health services. Additionally, efforts to improve water and sanitation facilities and promote education, particularly regarding nutrition awareness, are highlighted. Empowering women to delay and space pregnancies appropriately is emphasized as crucial for maternal and child health. Despite existing efforts, policies and programs in India have primarily focused on post-childbirth feeding interventions, overlooking the importance of addressing maternal nutrition during fetal development. Recognizing this gap, the paper highlights the paramount importance of addressing maternal nutrition to tackle the root causes of malnutrition and improve health outcomes for both mothers and children

Epidemiological Proileof Children with Malnutrition at Child Malnutrition Treatment Center (CMTC)in Saurashtra Region

Introduction: Adequate Nutrition is important for optimum growth, immunity, neurological and cognitive development of the child. Malnutrition leads to illness and mortality among under 5 children. Nearly 40% under 5 children are malnourished in Gujarat despite many nutritional programmes. Objectives: To assess the epidemiological factors of malnourished children at Child Malnutrition Treatment Centre (CMTC) and to ind outthe association between epidemiological factors and nutritional status of study participants. Method: A cross-sectional study was conducted on Children admitted at CMTC at Narayan Nagar Urban Health Center, the only one and newly established CMTC in Rajkot city in year 2015. Study duration was 14 months. Interview of mothers of 120 children was conducted to know epidemiological factors of malnourished children. Weight on admission was noted from register. Pre-formed, semi-structured proforma used. Results: Out of 120 children, 5.8%, 55.8%, 34.2% and 4.2% were from &lt;1, 1-3, 3-5 and &gt;5 year age groups respectively. Nearly 40% children belonged to Socioeconomic Class IV. The proportion of Low Birth Weight children was 40%. Current feeding practice was appropriate in 10.8% children according to IMNCI guideline. Upper Respiratory Tract Infection occurred in 76.7% children with average 4.5 episodes. High birth order of children is statistically signi icant with severity of malnutrition. Birth weight and feeding practices of children were not associated with grade of malnutrition. Conclusion: Need based education of mothers for preventing low birth weight, exclusive breastfeeding, complementary feeding, quantity and type of routine diet etc. should be given.

How Do Older Patients with End-Stage Osteoarthritis of the Hip Eat Prior to Hip Replacement? A Preliminary Snapshot That Highlights a Poor Diet

Diet quantity and quality in older adults is critical for the proper functioning of the musculoskeletal system. In view of hip surgery, old patients should consume 1.2–1.5 g of proteins and 27–30 kcal per kilo of body weight daily, and adhere to healthy eating habits. In this analytical study, we studied diet quantity and quality in relation to the clinical chemistry and functional status of 57 older adults undergoing elective hip replacement. Nine in ten patients did not meet suggested protein and energy intakes and only one in ten patients exhibited high adherence to the Mediterranean diet. Legume consumption adjusted for sex, age, body mass index, and health status successfully forecasted haemoglobin levels (p &lt; 0.05), and patients regularly consuming olive oil reported minor hip disability compared to those using it less frequently (p &lt; 0.05). Patients who reported daily ingestion of &lt;1 serving of meat versus those consuming &gt;1.5 servings had greater cumulative comorbidity (p &lt; 0.05), with meat consumption independently predicting walking ability, mobility, and balance in the fully adjusted model (p &lt; 0.01). In conclusion, our patients seem to eat poorly. There is room for improvement in pre-operative pathways to make older adults eat better, but there is a need to plan an interventional study to fully understand the cause–effect of a dietary pattern or specific food in enhancing recovery after surgery.