Dichromate Method Research Articles

Application of Industrial Wastes for the Production of Microbial Single-Cell Protein by Fodder Yeast Candida utilis

PurposeThe aim of this study was to determine the use of glycerol as a source of carbon and the use of deproteinized potato wastewater as a source of nitrogen and mineral components for the production of biomass by fodder yeast Candida utilis.MethodsThe yeast strain Candida utilis ATCC 9950 was used in this study. Experimental media contained potato wastewater and glycerol at concentrations of 5, 10, 15, 20, and 25% (w/v). Control medium was YPD and potato wastewater. During the 72 h of cultivation (200 rpm, 28 °C), the optical density (spectrophotometric method), biomass yield (weight method), glycerol (chemical method), and protein (Kjeldahl’s method) concentration in the experimental media, protein content in the biomass (Kjeldahl’s method) and chemical-oxygen demand index (dichromate method) in selected culture media were determined.ResultsCandida utilis was able to grow in the medium composed of potato wastewater and glycerol. Addition of 5% of glycerol gave a biomass yield above 30 gd.w./L and the efficiency of protein biosynthesis was 12.2 g/L. The results showed that the cultivation C. utilis in this medium caused a significant reduction of glycerol (83%), nitrogen present in potato wastewater (51%), and a high degree of COD index reduction (91%).ConclusionsThe study demonstrated that potato wastewater supplemented with glycerol may be successfully used as a carbon source in the production of fodder yeast C. utilis ATCC 9950 biomass. In addition, the results show that these two troublesome industrial wastes could be efficiently used in such processes.

Spatiotemporal Progression of Microcalcification in the Hippocampal CA1 Region following Transient Forebrain Ischemia in Rats: An Ultrastructural Study.

Calcification in areas of neuronal degeneration is a common finding in several neuropathological disorders including ischemic insults. Here, we performed a detailed examination of the onset and spatiotemporal profile of calcification in the CA1 region of the hippocampus, where neuronal death has been observed after transient forebrain ischemia. Histopathological examinations showed very little alizarin red staining in the CA1 pyramidal cell layer until day 28 after reperfusion, while prominent alizarin red staining was detected in CA1 dendritic subfields, particularly in the stratum radiatum, by 14 days after reperfusion. Electron microscopy using the osmium/potassium dichromate method and electron probe microanalysis revealed selective calcium deposits within the mitochondria of degenerating dendrites at as early as 7 days after reperfusion, with subsequent complete mineralization occurring throughout the dendrites, which then coalesced to form larger mineral conglomerates with the adjacent calcifying neurites by 14 days after reperfusion. Large calcifying deposits were frequently observed at 28 days after reperfusion, when they were closely associated with or completely engulfed by astrocytes. In contrast, no prominent calcification was observed in the somata of CA1 pyramidal neurons showing the characteristic features of necrotic cell death after ischemia, although what appeared to be calcified mitochondria were noted in some degenerated neurons that became dark and condensed. Thus, our data indicate that intrahippocampal calcification after ischemic insults initially occurs within the mitochondria of degenerating dendrites, which leads to the extensive calcification that is associated with ischemic injuries. These findings suggest that in degenerating neurons, the calcified mitochondria in the dendrites, rather than in the somata, may serve as the nidus for further calcium precipitation in the ischemic hippocampus.

A miniature photoelectrochemical sensor based on organic electrochemical transistor for sensitive determination of chemical oxygen demand in wastewaters

A three-electrode configuration is often required in the conventional photoelectrochemical measurements. Nevertheless, one common drawback is the reference electrode and the counter electrode used in the measurements, which has been proved to be an impediment for the miniaturization. In this study, a simple, cost-effective and miniature photoelectrochemical sensor based on high sensitive organic electrochemical transistor (OECT) is developed and used for the determination of chemical oxygen demand (COD) in wastewaters. The devices show detection limit down to 0.01 mg/L COD, which is two orders of magnitude better than that of the conventional photoelectrochemical method. The excellent sensing performance can be contributed to the novel sensing mechanism of OECT devices. That is, the devices are sensitive to the potential changes induced by the photoelectrochemical reaction on TiO2 nanotube arrays gate electrodes. Real sample analyses are also carried out. The results demonstrate that the measured COD values using the OECT devices and the standard dichromate methods are in a good agreement. Since the proposed sensor is constructed on a miniature transistor, it is expected that the device shows a promising application on the integrated COD monitoring platform.

A Positional Method for Accidental Chemical Oxygen Demand to Determine a River’s Pollution Source

This study stems from a current lack of technology to assess pollutantsources along rivers located in China. A positive water quality distribution model forpollution sources was established, which applies reverse tracing to investigate the likelysource of pollution, and a positional method of accidental chemical oxygen demand(COD, analyzed using the dichromate method GB11914-89, the COD in this papertherefore refers to CODcr) of the pollution source in a river was proposed. This methodis based on the COD degradation principle in a natural water body, where the variationin the concentration of pollutants in the controlling cross sections was calculated byestablishing a mathematical model. A set of calculated values for the controlling crosssections were determined by making educated assumptions about each accidental outlet.The calculated value and the monitored value in the same cross sections were thenmatched, and the outlets with calculated values that were closer to the monitored valueare likely to be the sources of accidental pollution. On this basis, the probability valuesof these matching outlets were calculated by error sequence.

Saccharification and Single Step Fermentation of Cassava Peel by Mixed Culture of Saccharomycopsis fibuligera NCIM 3161 and Zymomonas mobilis MTCC 92

The main objective of this study was to utilize the cassava peel by saccharification and single step fermentation (SSF) by mixed culture of Saccharomycopsis fibuligera NCIM 3161 and Zymomonas mobilis MTCC 92. Single step fermentation was performed in conical flasks with various concentrations of cassava peel (30, 50, 70 and 90 g/L), pH (3.5, 4.5 and 5.5), temperature (37, 47 and 57° C), reaction time (72, 720 and 168 h), inoculum size (5, 10 and 15 % v/v) and agitation speed (50, 100 and 150 rpm). Cell growth was identified by measuring optical density at 660 nm. The total reducing sugars were determined by centrifuging at 5000 rpm for 10 min by 3,5-dinitrosalicylic acid method. Starch concentration was estimated by anthrone method. Ethanol concentration was determined by acid dichromate method. The optimum process conditions were substrate concentration of 70 g/L, pH of 4.5, temperature of 37° C, reaction time of 120 h, inoculum size of 10 % (v/v) and agitation speed of 100 rpm. Under these conditions, the highest ethanol concentration of 26.46 g/L was obtained for cassava peel (93.75 % of theoretical yield).

Decolorization and decontamination of textile wastewater by gamma irradiation in presence of H2O2

Gamma irradiation is a contemporary concept for the treatment of textile wastewater. The organic pollutants and coloring materials of wastewater can be decomposed acceptably by gamma irradiation. However, the process efficiency can be improved significantly by integrating H2O2 as an additive with wastewater during irradiation. The treatment of combined textile wastewater collected from a knit dyeing industry was carried out using a Cobalt-60 gamma radiation source at 10 kGy irradiation dose with a dose rate of 13 kGy/h in presence of 0.5, 1.0, 2.0, and 3.0% concentrations of H2O2. Then the change in pH and decoloration percentage, reduction in total suspended solids (TSS), five-day biological oxygen demand (BOD5), and variation of chemical oxygen demand (COD) were extensively investigated. It was observed that colored wastewater become almost colorless due to destruction of the chromophore group of the dye molecules by irradiation. Moreover, the alkaline pH of wastewater is also reduced to neutral condition by fragmentation of larger dye macromolecules resulting in the formation of smaller acidic organic compounds. The degradation of the solid particles and refractory organic matters leads to a considerable reduction in TSS and BOD5 values and consequently the COD of wastewater after irradiation. However, the COD values were found to increase in presence of H2O2 by dichromate method as it behaves as a reductant and oxidized by potassium dichromate resulting in a significant increment in COD reading. So it can be summarized that the application of gamma irradiation with the incorporation of H2O2 is a promising tool for the treatment of textile wastewater by decontaminating its organic pollutants effectively. This effectual degradation of organic matter can eliminate environmental hazard significantly after discharging a large volume of irradiated wastewater to the environment with the minimum concentration of perilous organic matters and coloring substances.

A Novel Thermal Sensor for the Sensitive Measurement of Chemical Oxygen Demand.

A novel rapid methodology for determining the chemical oxygen demand (COD) based on a thermal sensor with a flow injection analysis system was proposed and experimentally validated. The ability of this sensor to detect and monitor COD was based on the degree of enthalpy increase when sodium hypochlorite reacted with the organic content in water samples. The measurement results were correlated with COD and were compared against the conventional method using potassium dichromate. The assay required only 5–7 min rather than the 2 h required for evaluation by potassium dichromate. The linear range was 5–1000 mg/L COD, and the limit of detection was very low, 0.74 mg/L COD. Moreover, this method exhibited high tolerance to chloride ions; 0.015 mol/L chloride ions had no influence on the response. Finally, the sensor was used to detect the COD of different water samples; the results were verified by the standard dichromate method.

Iron oxides as weathering indicator and the origin of Luvisols from the Vistula glaciation region in Poland

The aim of the research was to determine the effect of lithogenic and pedogenic processes on the formation of Luvisols from the area of Vistula glaciation on the base of profile distribution of iron oxides and total iron in relation to texture and physicochemical properties. The indices of weathering of the soil material in genetic horizons were calculated, and changes in the content and forms of iron oxides were evaluated. The predominant type of soil in the study area is Luvisols under agricultural use, formed from silt formations on loam. The analyses were made applying the following methods: grain size composition using the sieve method and hydrometer method, the interpretation of the results was performed according to the World Reference Base for Soil Resources classification, the pH of soils was measured with the potentiometric method, C-organic with the Walkley-Black dichromate method, the content of the following iron forms was determined (total iron (Fet) after the mineralization of soils in the mixture of HF and HClO4 acids), free iron oxides were extracted using dithionite-citrate-bicarbonate method, and amorphous iron oxides after the ammonium oxalate extraction (using the Philips 9100PU apparatus). The clay mineralogy was estimated by X-ray diffraction analysis. It was observed that total iron enrichment occurs in argic horizons accompanied by iron depletion in luvic horizons, while the profile distribution of iron is similar to the distribution of clay. The (Fed/Fet) ratio indicates a low degree of weathering; the highest values were observed in argic (Bt) horizons, which confirms the effect of the process of pedogenesis on the value of that index. In the soils investigated, crystalline iron oxides generally dominate over the amorphous forms. The mineralogical composition of clay fraction separated from the upper part of soils was different as compared to the underlying material. The results of the study showed that iron contents (together with the other indicators) and its forms can be used to distinguish soil layers of different origin. The depth distribution of Fed, Feo and Fet within soil profiles indicates that the soil material may be of different lithogenic origin in the studied pedons.

Ultrastructural investigation of microcalcification and the role of oxygen–glucose deprivation in cultured rat hippocampal slices

Intracellular calcium accumulation is associated with cell death in several neuropathological disorders including brain ischemia, but the exact mechanisms of calcification need to be clarified. We used organotypic hippocampal slice culture — cultures subjected to oxygen–glucose deprivation (OGD) mimicking the in vivo situation to investigate the events underlying ectopic calcification. Alizarin red staining indicating calcium deposition was observed in the cornu ammonis (CA)1 and dentate gyrus regions in control hippocampal slices despite no specific labeling for cell death markers. Electron microscopy using the osmium/potassium dichromate method revealed scattered degenerated cells throughout the normally appearing CA1 region. They contained electron-dense precipitates within mitochondria, and electron probe microanalysis confirmed that they were calcifying mitochondria. Selective calcium deposition was noted within, but not beyond, mitochondria in these mineralized cells. They showed ultrastructural features of non-necrotic, non-apoptotic cell death and retained their compact ultrastructure, even after the majority of mitochondria were calcified. Unexpectedly, no intracellular calcification was noted in necrotic CA1 pyramidal cells after OGD, and there was no progression of calcification in OGD-lesioned slices. In addition, mineralized cells in both control and OGD-lesioned slices were closely associated with or completely engulfed by astrocytes but not microglia. These astrocytes were laden with heterogeneous cytoplasmic inclusions that appeared to be related with their phagocytic activity. These data demonstrate that microcalcification specifically associated with mitochondria might lead to a novel type of cell death and suggest that astrocytes may be involved in the phagocytosis of these mineralized cells and possibly in the regulation of ectopic calcification.

Low cost chemical oxygen demand sensor based on electrodeposited nano-copper film

A commercially available copper electrical cable and pure Cu disk were used as substrates for the electrodeposition of copper nanoparticles (nano-Cu). The surface morphology of the prepared nano-Cu/Cu electrodes was investigated by scanning electron microscope (SEM) and energy dispersive X-ray spectrometer (EDX). The bare copper substrates and the nano-copper modified electrodes were utilized and optimized for electrochemical assay of chemical oxygen demand (COD) using glycine as a standard. A comparison was made among the four electrodes (i.e., bare and nano-Cu coated copper cable and pure copper disk) as potential COD sensors. The oxidation behavior of glycine was investigated on the surface of the prepared sensors using linear sweep voltammetry (LSV). The results indicate significant enhancement of the electrochemical oxidation of glycine by the deposited nano-Cu. The effects of different deposition parameters, such as Cu2+ concentration, deposition potential, deposition time, pH, and scan rate on the response of the prepared sensors were investigated. Under optimized conditions, the optimal nano-Cu based COD sensor exhibited a linear range of 2–595mg/L, lower limit of detection (LOD) as low as 1.07mg/L (S/N=3). The developed method exhibited high tolerance level to Cl− ion where 1.0M Cl− exhibited minimal influence. The sensor was utilized for the detection of COD in different real water samples. The results obtained were validated using the standard dichromate method.

Electrochemical nanocomposite-derived sensor for the analysis of chemical oxygen demand in urban wastewaters.

This work reports on the fabrication and comparative analytical assessment of electrochemical sensors applied to the rapid analysis of chemical oxygen demand (COD) in urban waste waters. These devices incorporate a carbon nanotube-polystyrene composite, containing different inorganic electrocatalysts, namely, Ni, NiCu alloy, CoO, and CuO/AgO nanoparticles. The sensor responses were initially evaluated using glucose as standard analyte and then by analyzing a set of real samples from urban wastewater treatment plants. The estimated COD values in the samples were compared with those provided by an accredited laboratory using the standard dichromate method. The sensor prepared with the CuO/AgO-based nanocomposite showed the best analytical performance. The recorded COD values of both the sensor and the standard method were overlapped, considering the 95% confidence intervals. In order to show the feasible application of this approach for the detection of COD online and in continuous mode, the CuO/AgO-based nanocomposite sensor was integrated in a compact flow system and applied to the detection of wastewater samples, showing again a good agreement with the values provided by the dichromate method.

Spectrophotometric Determination of Some Angiotensin Converting Enzyme Inhibitors By Potassium Dichromate and Potassium Permanganate in Tablet Dosage Form

Simple, cost effective and reproducible spectrophotometric methods are proposed for the determination of enalapril maleate, lisinopril dihydrate, moexipril hydrochloride and ramipril hydrochloride in pure and tablet doage forms. The methods are based on oxidation of these drugs by either potassium dichromate or potassium permanganate in sulphuric acid medium then measuring of the developed colored reaction products or the decrease in the intensity of color at λmax 610, 520 nm for the potassium dichromate (method A) and potassium permanganate (method B) respectively. Different variables affecting the reaction conditions were carefully studied and optimized. Under optimal experimental conditions the linear range is 20-900 μg.ml-1 (method A) and 2-500 μg.ml-1 (method B). The proposed methods were successfully applied to the analysis of the investigated drugs in pure and tablet dosage form. Results were comparable with those obtained by reported spectrophotometric methods.

Electrochemical tuning of the activity and structure of a copper-cobalt micro-nano film on a gold electrode, and its application to the determination of glucose and of Chemical Oxygen Demand.

Micro-nano structured Cu-Co was in situ fabricated on the surface of a gold electrode via electrochemical reduction of CuCl2 and Co(NO3)2. It is shown that the shape of the particles can be controlled by variation of deposition current, deposition time, pH value and the ratio of Cu(II) and Co(II) ions. If prepared under current of −200 μA in 0.1 M, pH 4.0 acetate buffer solution, the film possesses high catalytic activity towards the electrochemical oxidation of glucose at a largely increased oxidation current compared to a non-modified surface. The electrochemical activity of this sensor can be easily tuned. Glucose is a standard compound for evaluating the chemical oxygen demand (COD), and we have therefore studied the application of the sensor to the determination of this parameter. Under optimized conditions, the sensor has linear response to glucose in the 1.92-768 mg L−1 concentration range, and the detection limit is 0.609 mg L−1 (at an S/N ratio of 3). A large number of surface water samples was studied, and the results obtained by this method were found to be linearly correlated to those obtained by the dichromate method (r = 0.995; n = 33). Graphical This study describes the facile synthesis of micro-nano Cu-Co by one-step electrodeposition of Cu(II) and Co(II) on gold electrode. The alloy composite exhibited excellent electrocatalytic activities, and was successfully applied on the COD determination of glucose and water samples. Electronic supplementary materialThe online version of this article (doi:10.1007/s00604-014-1353-z) contains supplementary material, which is available to authorized users.

Rapid determination of the chemical oxygen demand of water using a thermal biosensor.

In this paper we describe a thermal biosensor with a flow injection analysis system for the determination of the chemical oxygen demand (COD) of water samples. Glucose solutions of different concentrations and actual water samples were tested, and their COD values were determined by measuring the heat generated when the samples passed through a column containing periodic acid. The biosensor exhibited a large linear range (5 to 3000 mg/L) and a low detection limit (1.84 mg/L). It could tolerate the presence of chloride ions in concentrations of 0.015 M without requiring a masking agent. The sensor was successfully used for detecting the COD values of actual samples. The COD values of water samples from various sources were correlated with those obtained by the standard dichromate method; the linear regression coefficient was found to be 0.996. The sensor is environmentally friendly, economical, and highly stable, and exhibits good reproducibility and accuracy. In addition, its response time is short, and there is no danger of hazardous emissions or external contamination. Finally, the samples to be tested do not have to be pretreated. These results suggest that the biosensor is suitable for the continuous monitoring of the COD values of actual wastewater samples.

Mineral-associated organic carbon and black carbon in restored wetlands

Stable soil organic carbon (SSOC) has a vital influence on soil's capacity for resisting disturbance and sequestering carbon. However, studies of the SSOC in restored wetlands are incomplete. We studied mineral-associated organic carbon (MOC) and black carbon (BC), two representatives of SSOC, in a wetland restoration chronosequence in Caizi Lake, East China. MOC was analyzed by both a physical method, involving dividing soil into size-fractions POM (>53 μm) and MOM (0.45–53 μm) and detecting OC in MOM (MOCP), and a chemical method, acidifying soil with hydrofluoric acid (HF) and detecting OC loss (MOCC). BC was analyzed by methods using dichromate (BCCr) and chemothermal oxidation (BCCTO). MOCC, BCCr, and BCCTO were further determined in MOM for comparing results from different fractions and methods. We found an elevation of soil ecological function in wetlands restored from farmlands in Caizi Lake, which was supported by a general increase of MOC and BC with time after restoration at surface soil (0–6 cm). Comparison of methods showed that size fractionation method overestimated MOC by identifying all OC in MOM as MOC, since HF-soluble OC accounted for no more than 23.5% of the whole OC in MOM. As it may separate more components, e.g. charcoal, from BC continuum, the dichromate method was recommended as more meaningful in the study of BC recovery in restored wetlands with biomass-burning history. We presented the possible use of ratio (BCCr–BCCTO):BCCTO for BC source identification which produced a reasonable result similar to that of a commonly used method. The combination of physical and chemical fractionation methods revealed that the proportion of BC to TOC in POM was considerable, which indicated that POM was not invariably labile as was commonly understood. Finally, a detailed list of soil organic carbon components including MOC, BC and labile OC in each size fraction was represented by using sequential analysis and combination of fractionation methods.

Galactose Consuming Microbes for Ethanol Production from Seaweed

Gracilaria sp. is one of macroalgae that contain high amount of sugar especially galactose. This galactose can be fermented into bioethanol by galactose-consuming microbes similar to the widely known ethanol production by yeast S.cerevisae. Hence the main objective of this study is to isolate galactose consuming microbes which capable of fermenting galactose into bioethanol. For this purpose, microbes-containing sample from seaweed culture were grown on galactose agar and tested for their survival as well as ethanol production capability. Seven isolated microbes belong to fungi and bacteria species were tested for their capability in fermenting galactose to produce ethanol. The concentrations of ethanol that have been produced by isolated microbes were analyzed by dichromate method whereas the consumption of galactose was determined by Dinitrosalicyclic acid (DNS) method. It was found that ethanol production resulted from fermentation by S1, S2, S3 and S4 were 0.80% (w/v), 0.74% (w/v), 0.81% (w/v) and 0.85% (w/v) respectively. Identification of these strains, as well as optimization of their ethanol fermentation are undergoing in our laboratory.

Electrochemical Oxidation of 2,4,6-Trinitrophenol on Ti/SnO<sub>2</sub>-Sb<sub>2</sub>O<sub>5</sub>/PbO<sub>2 </sub>Anode for Waste Water Treatment

The electrochemical oxidation of 2,4,6-trinitrophenol on Ti/SnO2-Sb2O5/PbO2electrode was studied by cyclic voltammetry in this paper. The electrochemical behaviour of the electrode in sodium sulfate solution and in the mixture solution of sodium sulfate and 2,4,6-trinitrophenol has been studied. The experimental results of cyclic voltammetry showed that acidic media was suitable for efficient electrochemical oxidation of 2,4,6-trinitrophenol. Some electro-generated reagent formed in the electrolysis process and 2,4,6-trinitrophenol could be oxidized by the electro-generated reagent. The Ti/SnO2-Sb2O5/PbO2electrode was used as the anode and the electrolysis experiment was carried out under the optimized conditions. The electrolysis process was monitored by UV-visible spectrometry and high performance liquid chromatography (HPLC), and the chemical oxygen demand (COD) was determined by the potassium dichromate method. The mechanism of 2,4,6-trinitrophenol be oxided was studied primarily by the cyclic voltammetry and UV-visible spectrometry. The results of electrolysis experiment demonstrated the possibility that the electrode was used as an anode for electrochemical treatment of 2,4,6-trinitrophenol contained in the waste water.

A visible light assisted photocatalytic system for determination of chemical oxygen demand using 5-sulfosalicylic acid in situ surface modified titanium dioxide

Titanium dioxide (TiO2) nanoparticles were in situ surface modified by the chemisorption of 5-sulfosalicylic acid as non-sacrificial organics and then used as a visible light active photocatalyst (5-SA-TiO2). Based on the photodegradation of organic pollutants, a new system, 5-SA-TiO2–KMnO4, was used for the first time for the determination of chemical oxygen demand (COD). A linear correlation was observed between the amount of oxidizable dissolved organic matter and the amount of MnO4− consumed by the coupled reduction process. Thus, COD could be determined by the depletion of MnO4−. After TiO2 was surface modified by 24.82μg/mg of 5-SA, the working calibration range was extended from 2–12mg/L and 50–300mg/L to 0.3–400mg/L and the disadvantages of pure TiO2 for COD detection techniques were overcome, including UV irradiation and low oxidation percentage. Without any sample pretreatment and toxic reagents, the COD values of nature water and real wastewater could be successfully determined by achieved technique. Excellent agreement between our proposed method and conventional dichromate method was achieved.

Electrochemical Oxidation of 4-Chlorophenoxyacetic Acid on Ti/SnO<sub>2</sub>-Sb<sub>2</sub>O<sub>5</sub>/PbO<sub>2</sub> Anode for Waste Water Treatment

The electrochemical oxidation of 4-chlorophenoxyacetic acid on Ti/SnO2-Sb2O5/PbO2 electrode was studied by cyclic voltammetry in this paper. The electrochemical behaviour of the electrode has been studied. The experimental results of cyclic voltammetry showed that acidic media was suitable for efficient electrochemical oxidation of 4-chlorophenoxyacetic acid. The Ti/SnO2-Sb2O5/PbO2 electrode was used as the anode and the electrolysis experiment was carried out under the optimized conditions. The electrolysis process was monitored by UV-visible spectrometry and high performance liquid chromatography (HPLC) and the chemical oxygen demand(COD) was determined by the potassium dichromate method. The mechanism of 4-chlorophenoxyacetic acid be oxided was studied primarily. The results of electrolysis experiment demonstrated the possibility that the electrode was used as an anode for electrochemical treatment of 4-chlorophenoxyacetic acid contained in the waste water.

Comparison with Determing Methods of Organic Matter for Sludge Compost in Different Treatments

the organic matter contents in deferent treatments of the sludge compost were determined by using Comparison the potassium dichromate with the burning method ,in order to understand changes of the organic matter contents for the Sewage sludge compost in adding different treatments of Fermentation accelerant and nutrient agents . The results showed as following: 1, the organic matter content for the sludge single composting at the end (53.36%) was lower 8.65% than at the beginning of composting(58.41%), the other corresponding treatment in composting all declined 12.37% -7.98%, indicating that the sludge compost would made The organic matter content are significantly reduced. 2, adding Fermentation accelerant into the sludge from 8% to 40%, the organic matter contents of their composting at the end increased more 2.94% ~ 19.73% than that of the single sludge compost. the highest organic matter content occurred in treatment 6 (sludge +40% Fermentation accelerant, 63.89 %). 3, at adding 24% Fermentation accelerant in the sludge ,as adding 8~32% of nutrient agents, the organic matter contents at the end of composting have been increased more 10.48%, 7.46%, 6.39% and 2.42% than the sludge single treatment , indicating that the sludge Adding 24% Fermentation accelerant and adding more nutrient agents can significantly improved the level of the organic content. 4, the organic matter contents for the sludge adding 30-50% nutrient agents have been declined 4.10% ~ 6.90% than the single-sludge compost treatment. 5, comparison of two measurement results showed that the potassium dichromate method is More advantages in measuring organic matter content of sludge compost than the burning method.