It has been well known that the use of ultracentrifuge as the step of the purification of influenza virus is the most effective method, however, as the use of the apparatus is so far fairly limited in our country, other methods for purifying viruses as pure as possible from the infected materials without losing their activities should be investigated. We reported in the previous paper (1) that the purification of influenza virus was achieved to some extent by means of absorption and elution using such absorbents as ion exchange resin, clay, kaolin, diatom earth, or chicken red cells, and furthermore, that the virus was purified to a considerably high degree by combining precipitation methods, preliminary purified by these adsorbents.Purification of various toxins as well as viruses by means of precipitation and elution employing ZnCl2, KAl(SO4)2, protamin sulfate, or isoelectric point precipitation, has been reported by many workers. Miyata (2) purified tetanus toxin by ZnCl2 and Hosoya (3) and Borily (4) reported that precipitation and elution by KAl (SO4) 2 was useful for the purification of diphtheria toxin (3) and influenza virus (4) respectively. Chambers and Henle (5), Warren et al(6), Warren (7), and Nakagawa (8) employed protamine sulfate to purify partially various viruses with considerably good results. Other wokers proved that vaccinia virus (9) and poliomyelitis virus (10) in infected tissues could be fairly purified by the isoelectric point precipitation method.The present paper shows the results of purification of influenza virus by means of combination of those methods, described above, and methanol precipitation method which was conducted similarly or with slight change to that recently reported by Cox (11) and Fukumi (12).
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