Stingless bee honey (SBH) is the most important hive product from stingless bee colonies with great economic value and health benefits. Nevertheless, there is little information on the physiochemical parameters of Chinese SBH, which also limits its utilization and quality control. From 2019 to 2023, we collected 89 Chinese SBH samples from 4 provinces, representing 5 local Chinese stingless bee species. We performed comprehensive assessments of various honey physicochemical indicators. These parameters include moisture, trehalulose, sucrose, fructose, glucose, total soluble solids, Baumé, ash, pH, electrical conductivity, 5-hydroxymethylfurfural, and acidity. Our data revealed that the physicochemical parameters of Chinese SBH are consistent with earlier reported results from other countries. Notably, the Chinese SBH exhibited a relatively low level of 5-hydroxymethylfurfural (0–9.64mg/kg) and sucrose (0–0.43g/100g). No diastase activity was detected among all these samples. Chinese SBH was rich in trehalulose (4.26–37.65g/100g). After comparing these indexes with published data and with Australian/Malaysian samples, we proposed a new food standard for regulations of Chinese SBHs. These findings provide crucial insights into the Chinese SBH and contribute significantly to developing quality control and global standards for SBH.