The aim of this study is to compare microleakage of cervical restorations using universal bonding and two-step self-etch adhesive with or without enamel etching through a dye penetration testing method. In this in vitro experimental study, 70 maxillary premolar teeth with no anomaly or decay were randomly divided into five groups of 14. Conventional cervical cavities were prepared with diamond bur on the facial surface of the teeth. The teeth were prepared using G-Premio Bond/self-etch technique, G-Premio Bond/total-etch technique, G-Premio Bond/selective-etch technique, Clearfil SE Bond/self-etch technique, and Clearfil SE Bond/selective-etch technique in Groups I to V, respectively, and restored with composite. Microleakage at the occlusal enamel margin and the cervical dentinal margin was ranked from 0 to 4 based on dye penetration using stereomicroscope (×32). Statistical analysis was carried out using Kruskal-Wallis and Mann-Whitney tests at a significance level of α = 0.05. The mean occlusal microleakage in the five study groups was 1.07, 0.57, 0.57, 1.28, and 0.78, respectively. The mean cervical microleakage was 0.97, 0.85, 0.64, 1.35, and 1.14, respectively. Kruskal-Wallis test showed significant difference between the microleakage scores of different groups in enamel (p = 0.022) and dentin (p = 0.01). Clearfil SE Bond/self-etch technique group showed the highest mean score of microleakage in enamel margins, and G-Premio Bond/total-etch technique and G-Premio Bond/selective-etch technique groups showed lowest enamel microleakage mean. Clearfil SE Bond/self-etch group showed the highest mean score of microleakage in dentin margins, while G-Premio Bond/selective-etch technique group showed the lowest mean score of dentin microleakage. Selective etching of enamel will decrease enamel microleakage. G-Premio Bond shows better microleakage results in comparison to Clearfil SE Bond in dentinal margins. A major problem in cervical composite restorations is the presence of microleakage in the cervical wall. Different adhesive systems have been designed to overcome this problem.
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