Nutrients are needed to increase crop production. Liquid fertilizer (bio-urine), which macro elements, easily absorbed by plants and quickly shows results with optimum concentration, is one of the organic fertilizers that can be used as a trigger for the growth of potted chrysanthemums. The aim of this study to determine the optimum concentration of bio-urine in potted chrysanthemum cultivation. This research was conducted from October to December 2020 at West Sumatera AIAT’s greenhouse on altitude of 1000 m above sea level, was designed with a Randomized Complete Block Design and five replications. Observed consist of five concentrations of bio-urine, namely (1) 200 ml L-1of water, (2) 100 ml L-1of water, (3) 66.67 ml L-1of water, (4) 50 ml L-1and (5) 40 ml L-1of water. Parameters observed were plant height, number and length of internodes, number of branches, stem diameter, flower initiation, number and diameter of flowers, and petiole length. The result shows that Bio-urine with a concentration of 100 ml L-1 of water produces potted chrysanthemum with optimum parameters of plant height, number of internodes, number of branches, stem diameter, number of flowers, flower diameter, and petiole length.