Enlargement Of Diameter Research Articles

Complexity of Inflammation in the Trajectory of Vascular Disease: Interleukin 6 and Beyond

Takayasu's arteritis, first described by Dr. Mikito Takayasu in 1908, is a systemic vasculitis that mostly affects the aorta and its major branches. Although the etiology of the disease is yet unknown, genetic and environmental factors may both play a role. One hundred years after the discovery of Takayasu's arteritis, inflammation is finally widely recognized as a fundamental condition common to all vascular diseases, and clinical trials have proven the efficacy of molecularly targeted drugs that block each step of the NLRP3 inflammasome/interleukin (IL)-1β/IL-6 cascade in patients with atherosclerotic vascular disease and elevated C-reactive protein (CRP). Recent advances have also been made in the treatment of Takayasu's arteritis. The randomized controlled trials and subsequent open-label and post-marketing surveillance studies in Japan have demonstrated that tocilizumab, an anti-IL-6 receptor antibody, is effective in the treatment of Takayasu's arteritis and prevents relapse during tapering of prednisolone doses. IL-6 is also heavily engaged in the remodeling of large vessels after acute aortic dissection as demonstrated in animal studies. In patients with acute aortic dissection, those with markedly elevated CRP levels in the acute phase are known to have an increased risk of aorta-related events, such as rupture due to aortic diameter enlargement, in the subacute and chronic phases. We discovered that elevated CRP levels following aortic dissection are caused by IL-6, which is produced by neutrophils that infiltrate the adventitia of the dissected aorta. In a mouse model of acute aortic dissection, we showed that IL-6 produced by these neutrophils causes progressive destruction of the arterial wall structure and that blockade of IL-6 signaling can prevent post-dissection vascular remodeling and improve life outcome. Therefore, inhibiting IL-6 signaling is anticipated to be effective in the secondary prevention of myocardial infarction and suppression of vascular modeling after dissection and even as an anti-inflammatory therapy for Takayasu's arteritis; however, this approach does not solve everything. Undoubtedly, the mechanisms of inflammation in vascular disease are diverse and complex, and the cytokines and cell populations involved at each site (coronary artery vs. aorta) and in each phenotype (atherosclerosis vs. aortic aneurysm vs. aortic dissection) need to be understood for each type of inflammation. Osteopontin (OPN) is a recruiter of monocytes and macrophages, induces cellular immune responses as a Th1 cytokine, acts as a fibrosis-promoting factor, and has been shown to be deeply involved in the pathogenesis of vascular diseases. We have shown that senescent T cells, which emerge with obesity and aging, secrete significant amounts of OPN, leading to metabolic abnormalities and chronic inflammation. Neutrophil extracellular traps (NETs) released from activated neutrophils have been shown to contribute to the pathogenesis of acute coronary syndromes (ACS) by interacting with macrophages, platelets, and vascular endothelial cells and thus promoting plaque erosion and immunothrombosis. In addition to standard anticoagulant and antiplatelet therapies, the effectiveness of anti-immunothrombotic therapies targeting NETs as a new preventive and therapeutic approach for ACS will be examined in the future.

Effect of heterogeneity size on three-phase contact line formation kinetics

Flotation relies on the differences in surface wettability. Although solids with the same hydrophobicity are generally regarded as having the same capture probability, the heterogeneities found on particle surfaces could complicate bubble–solid interactions. For surfaces with the same hydrophobicity and different sizes of inclusions, the heterogeneity size is expected to affect bubble–solid interactions. In this work, Microelectromechanical system techniques were used to fabricate chemically heterogeneous samples with the same hydrophobicity on which discrete hydrophilic dots with different diameters were distributed. The collision and adhesion processes between free-rising bubbles and these chemically heterogeneous surfaces were studied using a high-speed video camera. For all surfaces, the time intervals between successive collisions and the bubble trajectories were similar before bubble rupture. In contrast, the time elapsed between maximum air bubble deformation and rupture varied, with the rupture time appearing random. Nevertheless, the terminal diameter and average expansion velocity after bubble rupture were negatively correlated with the hydrophilic dot diameter. Thus, for heterogeneous surface with discrete hydrophilic dots, the bouncing behaviour of air bubbles before rupture is independent of heterogeneity size, whereas the three-phase contact line expansion behaviour after rupture is related to heterogeneity size. These insights can provide guiding principles for improving the flotation.

Precise Internal Turning of Axi-symmetric SS316 Thin-walled Component: Experimentations on ED-Machining Setup

ABSTRACT Axis-symmetric thin-walled cylindrical components like couplers and concentric reducers are needed in mechanical assemblies. These components do not have enough rigidity to hold in the chuck for conventional machining. It is difficult to control their radial dimensions accurately and cost-effectively. In this work, an attempt is made to enlarge the diameter of axis-symmetric thin-walled cylindrical components. Electric discharge machining (EDM) with a centreless turning attachment is utilised. Three variables were selected as input variables: impulse current (IP), pulse on time (Ton), and pulse off count (Toff count). Workpiece RPM, electrode gap, spark, lift, and machining time were constant parameters. Response surface methodology (RSM) was used in planning the experiments. The response variables were material removal rate (MRR), enlargement in diameter (EN), and circularity (Cir). It was found that MRR and EN increase with the increase in IP and Ton and decrease with the rise in Toff time. The values of optimum process parameters found at impulse current (15 A), pulse on time (490 µs) and pulse off count (6) were MRR (366 mg/min), enlargement in diameter (229 µm) and circularity (0.014 µm). Moreover, the sample’s topography changed as hardness and corrosion resistance increased.

Platelet pannexin-1 channels modulate neutrophil activation and migration but not the progression of abdominal aortic aneurysm.

Abdominal aortic aneurysm (AAA) is a common disease and highly lethal if untreated. The progressive dilatation of the abdominal aorta is accompanied by degradation and remodeling of the vessel wall due to chronic inflammation. Pannexins represent anion-selective channels and play a crucial role in non-vesicular ATP release to amplify paracrine signaling in cells. Thus, pannexins are involved in many (patho-) physiological processes. Recently, Panx1 channels were identified to be significantly involved in abdominal aortic aneurysm formation through endothelial derived Panx1 regulated inflammation and aortic remodeling. In platelets, Panx1 becomes activated following activation of glycoprotein (GP) VI. Since platelets play a role in cardiovascular diseases including abdominal aortic aneurysm, we analyzed the contribution of platelet Panx1 in the progression of abdominal aortic aneurysm. We detected enhanced Panx1 plasma levels in abdominal aortic aneurysm patients. In experimental abdominal aortic aneurysm using the pancreatic porcine elastase (PPE) mouse model, a major contribution of platelet Panx1 channels in platelet activation, pro-coagulant activity of platelets and platelet-mediated inflammation has been detected. In detail, platelets are important for the migration of neutrophils into the aortic wall induced by direct cell interaction and by activation of endothelial cells. Decreased platelet activation and inflammation did not affect ECM remodeling or wall thickness in platelet-specific Panx1 knock-out mice following PPE surgery. Thus, aortic diameter expansion at different time points after elastase infusion of the aortic wall was unaltered in platelet-specific Panx1 deficient mice suggesting that the modulation of inflammation alone does not affect abdominal aortic aneurysm formation and progression. In conclusion, our data strongly supports the role of platelets in inflammatory responses in abdominal aortic aneurysm via Panx1 channels and adds important knowledge about the significance of platelets in abdominal aortic aneurysm pathology important for the establishment of an anti-platelet therapy for abdominal aortic aneurysm patients.

Is human bone matrix a sufficient augmentation method revising loosened pedicle screws in osteoporotic bone? – A biomechanical evaluation of primary stability

IntroductionDespite good screw anchorage and safe screw trajectory, screw loosening occurs in several cases, especially in osteoporotic individuals. The aim of this biomechanical analysis was to evaluate the primary stability of revision screw placement in individuals with reduced bone quality. Therefore, revision via enlarged diameter screws was compared to the use of human bone matrix as augmentation to improve the bone stock and screw coverage. Methods11 lumbar vertebral bodies from cadaveric specimens with a mean age of 85.7 years (± 12.0 years) at death were used. 6.5 mm diameter pedicle screws were inserted in both pedicles and hereafter loosened using a fatigue protocol. Screws were revised inserting a larger diameter screw (8.5 mm) in one pedicle and a same diameter screw with human bone matrix augmentation in the other pedicle. The previous loosening protocol was then reapplied, comparing maximum load and cycles to failure between both revision techniques. Insertional torque was continuously measured during insertion of both revision screws. FindingsThe number of cycles and the maximum load until failure were significantly greater in enlarged diameter screws than in augmented screws. The enlarged screws' insertional torque was also significantly higher than of the augmented screws. InterpretationHuman bone matrix augmentation does not reach the same ad-hoc fixation strength as enlarging the screw's diameter by 2 mm and is therefore biomechanically inferior. Regarding the immediate stability, a thicker screw should therefore be prioritised.

Tricuspid annular dynamics, not diameter, predicts tricuspid regurgitation after mitral valve surgery: Results from a prospective randomized trial

ObjectiveCurrent guidelines advise using prophylactic tricuspid valve annuloplasty during mitral valve surgery, especially in the presence of annular diameter enlargement. However, several retrospective studies and a prospective randomized study from our department could not confirm that diameter enlargement is predictive of late regurgitation. We examined whether 2- and 3-dimensional echocardiographic and clinical characteristics could identify patients who will develop moderate or severe recurrent tricuspid regurgitation. MethodsPatients with less than severe functional tricuspid regurgitation (FTR) were randomized not to receive tricuspid annuloplasty, and 11 of 53 of them were excluded from the study because 3-dimensional echocardiographic analysis was not possible. Cox regression was used to estimate the model-based probability of moderate or severe FTR (vena contracta ≥3 mm) or progression of TR and FTR regression using valve dimensions (annulus area, diameter perimeter, nonplanar angle, and sphericity index), dynamics (annulus contraction, annulus displacement, and displacement velocity), and clinical parameters as possible predictors. ResultsAt a median follow-up of 3.8 years (range, 3-5.6 years), 17 patients had moderate or severe FTR or progression, and 13 had FTR regression. Our models identified annular displacement velocity as a significant predictor for FTR recurrence and nonplanar angle as a significant predictor for FTR regression. ConclusionsAnnular dynamics, not the dimension, predict recurrence and regression of FTR. Annular contraction should be systematically investigated as a possible surrogate of right ventricle function to prophylactically treat the tricuspid valve.

GRMWBO: design of a general-purpose repowering model for wind farms via bioinspired optimization

Wind farm optimization requires efficient design of placement models which can assist in identification of deployment locations for higher conversion efficiency. To perform this task, various methods are proposed by researchers, and most of them are applicable for new deployments. Due to limited availability of wind farm sites and cost constraints, it is impractical to replace old equipments. Thus, repowering of wind farms is necessary, and must be planned under existing deployment constraints. Existing repowering methods apply static modelling techniques, which limits their scalability and usability for real-time deployments. To overcome this limitation, a novel general-purpose repowering model via bioinspired optimization is discussed in this text. The model proposes design of a genetic algorithm that considers inflow wind speed, wind direction angle, height of hub, surface roughness, horizontal-axis wind turbine rotor diameter, thrust coefficient, turbine efficiency, air density, and wake expansion rate to estimate location of wind-based generators. These locations are optimized for maximum output power, and minimum deployment costs, which can be estimated for multiple farm areas. This text also proposes various case studies and hypothesis about these deployments, and showcases performance optimizations w.r.t. optimum placement for a given number of turbines. Based on this evaluation, it was observed that the proposed GRMWBO model is capable of improving power efficiency by 18.5%, reduce cost by 8.3%, and reduce number of wind-based generators by 2.9% when compared with various state-of-the-art models. It was also observed that the proposed model showcased consistent performance under different use cases. Thus, making it applicable for a wide variety of real-time deployments.

Homoepitaxial growth of 3-inch single crystalline AlN boules by the physical vapor transport process

Single crystalline aluminum nitride (sc-AlN or AlN) boules with a diameter of 3-inch (Φ76 mm) were successfully prepared by the physical vapor transport (PVT) process. The initial homoepitaxial growth run was performed on an aluminum nitride seed sliced from a Φ51 mm aluminum nitride boule, and diameter enlargement was conducted iteratively via the lateral expansion technique until a Φ76 mm boule was achieved. During the diameter expansion growth runs, the crystal shape transitioned from a hexagonal pyramid to a cylindrical pyramid. After the standard slicing and wafering processes, the as-obtained substrates were characterized by high-resolution X-ray diffraction (HRXRD), preferential chemical etching, and optical spectroscopy. The characterization results revealed that the aluminum nitride substrates showed good crystallinity and excellent UV transparency, although a slight quality deterioration was observed when the crystal size was expanded from Φ51 to Φ76 mm, while the deep-UV (DUV) transparency remained very similar to that of the aluminum nitride seeds. The Φ76 mm aluminum nitride boules obtained in this study are an important milestone towards achieving Φ100 mm (4-inch) aluminum nitride, which are essential for the rapid commercialization of deep-UV optoelectronics and ultra-wide bandgap (UWBG) electronics.

Synthesis and properties of COK‐12 large‐pore mesocellular silica foam

AbstractLarge, ink‐bottle‐shaped pores in mesocellular foams (MCFs) are desired for various applications requiring enhanced mass transfer or the immobilization of larger compounds. Hence, the cylindrical pores of COK‐12, an ordered mesoporous silica structurally comparable to SBA‐15 but synthesized at room‐temperature at quasi‐neutral pH, are chemically swollen into ink‐bottle pores. Therefore, p‐xylene is used as a more sustainable swelling agent compared to popular alternatives. Its high boiling point allows for an additional thermal aging step to amplify the mesostructure enlargement without needing additional chemicals. For COK‐12, the MCFs obtained at room temperature reach an unprecedented plateau for the modal mesopore cell and window diameter of 19.9 and 5.5 nm, respectively, with an underlying broad pore size distribution and distorted hexagonal lattice up to 14.5 nm, involving hexagonal and spherical structures. The combined chemical and thermal swelling resulted in the selective enlargement of the window diameter to more than 200% and a slightly increased cell diameter, pore size distribution, and hexagonal lattice distortion in comparison to the room temperature synthesis. Such materials are thought to be promising alternatives to SBA‐15‐based MCFs, often utilizing toxic catalysts during synthesis. The presented results pave the way for enhanced adsorptive, catalytic, and drug delivery performances for COK‐12‐based materials.

Fusion splicing of hollow-core to standard single-mode fibers using a gradient-index bridge fiber

High-performance interconnection between hollow-core fiber and conventional solid-core fiber is of great significance for a lot of promising applications of hollow-core fibers. The current problems for high-performance hollow-core fiber/solid-core fiber interconnection mainly involve mode field mismatch of the two fibers and the complex splicing process. Subject of study. We study fusion splicing of anti-resonant hollow-core fiber with low loss (0.52 dB) and conventional single-mode fiber using a convenient graded-index bridge fiber. Purpose of the work. We aim to eliminate the mode field diameter mismatch between anti-resonant hollow-core fiber and single-mode fiber and simplify the mode field diameter adaptation method and process. Method. We employ a convenient graded-index bridge fiber approach, where a gradient index fiber is first spliced with the single-mode fiber, and the mode interference within the graded-index bridge fiber causes periodic enlargement and shrinkage of the mode field diameter. By precise cutting of the graded-index bridge fiber, the mode field diameter can be well controlled and made approximately equal to the mode field diameter of the anti-resonant hollow-core fiber. After mode field diameter adaptation, an optimized arc discharge fusion splicing procedure is applied for the anti-resonant hollow-core fiber/single-mode fiber fusion splicing. Main results. A fusion splicing loss of about 0.52 dB is achieved, which involves nearly 0.42 dB mode-field-diameter-mismatch-induced loss and about 0.1 dB fusion splicing loss that is caused by the deformation of the anti-resonant hollow-core fiber. Practical significance. The developed approach is convenient and cost-effective, which can benefit a lot of applications of anti-resonant hollow-core fibers in future telecommunications, fiber gas lasers as well as fiber-optic sensing.

Synthesis of graphene derivatives from asphaltenes and effect of carbonization temperature on their structural parameters.

A method for synthesizing graphene derivatives from asphaltene is proposed in this work. The graphene derivatives are mainly composed of few-layer graphene-like nano-sheets of randomly distributed heteroatoms; mainly sulfur and nitrogen. The proposed method is based on a thermal treatment in which asphaltene is carbonized in a rotating quartz-tube furnace under an inert atmosphere at a temperature in the range of 400-950 °C. Asphaltenes from different origins were employed to verify the synthesis method. The results indicate that graphene derivatives obtained at high carbonization temperature have similar structural parameters, despite the evident differences in parent asphaltenes structures and compositions. The transformation of asphaltene to graphene derivatives mainly occurred due to three factors: the reduction in the average number of aromatic layers (n), the expansion in aromatic sheet diameter (L a), and the elimination of alkyl side chains. The reduction in the number of aromatic sheets per stack is primarily ascribed to thermal exfoliation, while the increase in the aromatic sheet diameter is attributed to secondary reactions in the aromatic core of asphaltene. The elimination of side chains, on the other hand, is mainly credited to thermal cracking. The quantification of defect density (L D) in the graphene derivatives suggests an association between defects and heteroatoms presence.

Mechanistic insights of magnolol antimicrobial activity against Mycoplasma using untargeted metabolomic analyses.

The unreasonable use of antibiotics is one of the important causes of antimicrobial resistance (AMR) that poses a huge public health threat. Magnolol is a traditional Chinese medicine exhibiting antibacterial-, antifungal-, anti-inflammatory-, and antioxidant activities. However, it is unclear whether magnolol has an inhibitory effect on mycoplasma. This study found that magnolol showed excellent inhibitory activity against various mycoplasmas. Magnolol showed dose-dependent inhibition of Mycoplasma synoviae growth and biofilm formation in vitro. Magnolol caused severely sunken and wrinkled M. synoviae cell membranes at the minimum inhibitory concentration, and an enlarged cell diameter. The chicken embryo infection model showed that magnolol significantly reduced M. synoviae pathogenicity in vivo. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the citrate cycle, glycolysis/gluconeogenesis, and pyruvate metabolism were significantly disturbed at the minimum inhibitory concentration of magnolol. Interestingly, 41% of differential metabolites were in the categories of lipids and lipid-like molecules. Protegenin A was up-regulated 58752-fold after magnolol treatment. It belongs to fatty acyls, and destroys cell membrane integrity and cell activity. Ghosphatidylethanolamine, phosphatidylglycerol, phosphatidic acid, and phosphatidylserine related to membrane maintenance and stress response were widely down-regulated. Collectively, our results illustrate the feasibility of magnolol as a phytochemical compound to treat mycoplasma infection.

Novel preparation of metal-free carbon xerogels under acidic conditions and their performance as high-energy density supercapacitor electrodes.

The development of metal-free supercapacitor electrodes with a high energy density is a crucial requirement in the global shift towards sustainable energy sources and industrial pursuit of an optimal supercapacitor. Indeed, from an industrial perspective, time assumes a paramount role in the manufacturing process. A majority of synthesis methods employed for the fabrication of carbon xerogel-based supercapacitor electrodes are characterized by prolonged durations, and result in relatively poor energy and power density. These limitations hinder their practical applications and impede their widespread manufacturing capabilities. In this study, carbon xerogel-based supercapacitor electrodes were made in the shortest time ever reported by making the condition highly acidic with hydrochloric acid (HCl). Furthermore, the investigation of the effect of HCl concentrations (0.1 M, 0.05 M, and 0.01 M) on the morphology and electrochemical behavior of the prepared samples is reported herein. Interestingly, the highest concentration of HCl developed the highest BET surface area, 1032 m2 g-1, which enforced the capacitive behavior to deliver a specific capacitance of 402 F g-1 at 1 A g-1 and a capacitance retention of 80.8% at a current density of 2 A g-1 in an electrolyte containing 0.5 M H2SO4 + 0.5 M Na2SO4. Moreover, an impressive energy density of 45 W h kg-1 at a power density of 18.2 kW kg-1 was achieved. Interestingly, as the HCl concentration increased, the equivalent series resistance decreased to 3.9 W with carbon xerogel 0.1 M HCl (CX0.1). The superior performance of CX0.1 may be attributed to its enlarged BET surface area, pore volume, pore diameter, and smaller particle size. This work provides a facile approach for the large-scale production of metal-free carbon supercapacitor electrodes with improved performance and stability and opens novel horizons to explore the impacts of many types of catalysts during the carbon xerogel preparation.

Correlation of Sonographic Classification of Neck Adenopathy (A-RADS) and Malignancy.

Cervical adenopathy can be involved in various pathological processes. This study aimed to evaluate the ultrasound classification of cervical adenopathy (A-RADS) to choose the appropriate approach. This cross-sectional study was conducted among 294 patients with cervical adenopathy at Mashhad University of Medical Sciences during 2020-2021. The data of the long axis diameter, short axis diameter, shape, border, vascular pattern, presence of calcification and changes in cyst/necrosis, cortical echogenicity, hilum visibility, and location of involved lymph nodes were extracted. Lymph nodes was classified into four normal, reactive, suspicious & lymphoid disorders, and metastatic groups, based on ultrasound appearance (Adenopathy-reporting and data system). Diagnostic methods included follow-up, core needle biopsy (CNB), and fine needle aspiration (FNA), and surgical results. After determining the final diagnosis, demographic, sonographic, and pathological data were analyzed at a significance level of p<0.05. Of 294 patients, 185 were benign, and 109 were malignant. There were no significant differences in the location, long axis diameter, shape, cystic or necrotic changes, calcification, and margins of the lymph nodes between the benign and malignant groups. The enlarged short axis diameter, invisible hilum with isoechoic cortex, and non-hilar vascularity were significantly higher in the malignant group (p<0.001). The malignancy rate was 8.7% in reactive cases, 48.5% in lymphoid disorders, and 90% in metastatic nodes. The results of this study shows that cervical lymph nodes can be classified based on short axis diameter, cortex and hilum echo-texture and vascular pattern into normal, reactive, suspicious & lymphoid disorders, and metastatic, which have a high concordance with pathologic results.

Mechanisms and efficacy of metformin-mediated suppression of established experimental abdominal aortic aneurysms

ObjectiveMetformin treatment attenuates experimental abdominal aortic aneurysm (AAA) formation, as well as reduces clinical AAA diameter enlargement in patients with diabetes. The mechanisms of metformin-mediated aneurysm suppression, and its efficacy in suppressing established experimental aneurysms, remain uncertain. MethodsExperimental AAAs were created in male C57BL/6J mice via intra-aortic infusion of porcine pancreatic elastase. Metformin alone (250 mg/kg), or metformin combined with the 5′ AMP-activated protein kinase (AMPK) antagonist Compound C (10 mg/kg), were administered to respective mouse cohorts daily beginning 4 days following AAA induction. Further AAA cohorts received either the AMPK agonist AICA riboside (500 mg/kg) as positive, or vehicle (saline) as negative, controls. AAA progression in all groups was assessed via serial in vivo ultrasonography and histopathology at sacrifice. Cytokine-producing T cells and myeloid cellularity were determined by flow cytometric analyses. ResultsMetformin limited established experimental AAA progression at 3 (−85%) and 10 (−68%) days following treatment initiation compared with saline control. Concurrent Compound C treatment reduced this effect by approximately 50%. In metformin-treated mice, reduced AAA progression was associated with relative elastin preservation, smooth muscle cell preservation, and reduced mural leukocyte infiltration and neoangiogenesis compared with vehicle control group. Metformin also resulted in reduced interferon-γ-, but not interleukin-10 or -17, producing splenic T cells in aneurysmal mice. Additionally, metformin therapy increased circulating and splenic inflammatory monocytes (CD11b+Ly-6Chigh), but not neutrophils (CD11b+Ly-6G+), with no effect on respective bone marrow cell populations. ConclusionsMetformin treatment suppresses existing experimental AAA progression in part via AMPK agonist activity, limiting interferon-γ-producing T cell differentiation while enhancing circulating and splenic inflammatory monocyte retention.

MODERNIZATION OF THE KHARKIV MICROWAVE SPECTROMETER: CURRENT STATE

Subject and Purpose. Results are presented of the recent considerable upgrade implemented at the Kharkiv microwave spectrometer. The upgrade has been aimed at extending the operating frequency range and increasing the utmost accessible spectral resolution of the spectrometer. Methods and Methodology. In order to extend the frequency range we have designed and constructed new BWO-based oscillator units, also providing for possibility of frequency tripler application. Construction of a new absorbing cell of enlarged diameter allowed us to considerably improve the spectral resolution for Lamb-dip measurements. Results. Owing to the upgrade, the spectrometer has become able to cover the frequency range from 34 to 420 GHz, with a gap from 183 to 200 GHz. The spectral resolution in the Lamb-dip observation mode has been increased by a factor of two. In addition, the functionality of the spectrometer has been significantly improved via modernization of several of its subsystems. Conclusions. The new upgrades of the spectrometer systems have permitted extending the operational frequency range and increasing the utmost accessible resolution by means of reducing the time-of-flight line broadening in the Lamb-dip measurements. In addition, application of square-wave frequency modulation with accurately known modulation parameters, in combination with careful modeling of the distortions caused by reflections in the absorbing cell, has allowed us to significantly improve the accuracy of line frequency measurements.

The Clinical Importance of Optic Nerve Sheath Diameter in Patients with Traumatic Brain Injury: Preliminary Report

Traumatic brain injury (TBI) is a serious health problem that is related to an increased mortality. In cases of severe TBI, the prediction of prognosis is essential. The enlargement of the optic nerve sheath diameter (ONSD) shows an increased intracranial pressure and is associated with poor outcomes. In this study, we aimed to evaluate the prognostic value of ONSD in patients with severe TBI. Forty-four patients with severe TBI were retrospectively enrolled in the study. The patients were divided into two groups: survivors (n=17) and non-survivors (n=27). Baseline characteristics, clinical data, Glasgow coma scale (GCS) on hospital admission, brain computed tomography (CT) results, injury severity score (ISS), and Marshall score were recorded for all patients. ONSD was calculated at 3 mm distance from the globe, immediately below the sclera. The ONSD on the initial CT was significantly higher in non-survivors compared with survivors (6.83±1.40 vs. 6.40±1.36, p<0.05). In addition, ISS and Marshall score were significantly higher, whereas GCS was significantly lower in non-survivors. ONSD was positively correlated with Marshall score (r=0.332, p<0.05). Receiver operating characteristics analysis demonstrated that ONSD ≥6.61 had a sensitivity of 70.4% and specificity of 64.7% for predicting mortality. It was shown that ONSD ≥6.61 had a 4.3-fold increased risk for in-hospital mortality (odds ratio: 4.35; 95% confidence interval: 1.195-15.865; p<0.05). The enlargement of ONSD on initial CT was detected to be associated with increased in-hospital mortality in patients with severe TBI.

A new chronology from debiased crater densities: Implications for the origin and evolution of lunar impactors

The giant planets are believed to experience dynamic instability, delivering numerous impactors (asteroids, comets and leftover planetesimals) to the Moon. Dynamic simulations show that the impacts of leftover planetesimals decay much quicker than those from the main belt. Due to the relatively slow decay of early lunar impact flux directly inferred from observed crater densities, the dominant impactors were believed to have always been the main belt asteroids instead of rapidly decreasing leftover planetesimals. However, previously-derived densities are biased, with topographic degradation thought to increase the apparent density over time due to degradation-induced enlargement of crater diameter, coeval targets with various properties exhibiting different crater densities, and preferential erasure of small craters by the formation of larger ones. Here we establish a model with consideration of all these effects to derive crater densities on a competent-rock-like reference target (denoted as debiased crater densities), showing a constant flux prolonged back to ∼3.8 billion years ago (Ga) and a more intense earlier impact flux by a factor up to 100 compared with the standard production and chronology functions. We find that ancient (older than ∼3.8 Ga) craters were dominantly produced by leftover planetesimals rather than asteroids, consistent with the early migration of the giant planets.

Effect of ApoE ε4 gene polymorphism on the correlation between serum uric acid and left ventricular hypertrophy remodeling in patients with coronary heart disease.

Hyperuricemia and dyslipidemia are associated with left ventricular hypertrophy (LVH), while the effect of ApoE gene polymorphism on the correlation between serum uric acid (UA) level and severity of LVH in patients with coronary heart disease (CHD) has not been clarified. This was a retrospective observational study of patients with CHD. Patients were divided into groups of ε4 carriers and non-ε4 carriers based on sanger sequencing. The association of ApoE ε4 gene polymorphism, serum UA level, and LVH, determined by cardiac color Doppler ultrasound, was evaluated by multivariate analysis. A total of 989 CHD patients who underwent ApoE genotyping were enrolled and analyzed. Among them, the frequency of the ApoE ε4 genotype was 17.9% (15.7% for E3/4, 1.1% for E4/4, and 1.1% for E2/4). There were 159 patients with LVH, 262 with end-diastolic LV internal diameter (LVEDD) enlargement, 160 with left ventricular ejection fraction (LVEF) reduction, and 154 with heart failure. Multivariate analysis showed that for every increase of 10 μmol/L in serum UA level, the risk of LVH decreased in ε4 carriers (odds ratio (OR) = 0.94, 95% confidence interval (CI): 0.890-0.992, P = 0.025) and increased in non-ε4 carriers (OR = 1.03, 95% CI: 1.005-1.049, P = 0.016). The risk of LVEDD enlargement tended to decrease in ε4 carriers (OR = 0.98, 95% CI: 0.943-1.023, P = 0.391) and increased in non-ε4 carriers (OR = 1.03, 95% CI: 1.009-1.048, P = 0.003). The risk of LVEF reduction was reduced in ε4 carriers (OR = 0.996, 95% CI: 0.949-1.046, P = 0.872) and increased in non-ε4 carriers (OR = 1.02, 95% CI: 0.994-1.037, P = 0.17). The risk of LVEDD enlargement decreased in ε4 carriers (OR = 0.98, 95% CI: 0.931-1.036, P = 0.508) and increased in non-ε4 carriers (OR = 1.02, 95% CI: 0.998-1.042, P = 0.07). High serum UA levels decreased the risk of LVH in ApoE ε4 carriers with CHD, while increased the risk of LVH in non-ε4 carriers.

Role of growth hormone in orthodontics

Human growth hormone (Somatotropin) is produced by acidophilic growth hormone cells in the anterior pituitary gland, stimulated by hypothalamus. Its production is regulated by several complex feedback mechanisms in response to stress, exercise, diet, sleep and growth hormone itself. Growth hormone plays a major role in linear body growth and craniofacial growth. In Orthodontics, it induces orthodontic tooth movement by activating specific cytokines to regulate osteoclast &amp; osteoblast activity. It mediates STAT signalling in liver &amp; pancreas to produce JAK-IGF-1. IGF-1 has stimulatory effects on osteoblasts and chondrocytes for endochondral ossification to promote bone growth. Growth hormone mediates mitogenic stimulus to odontoblast lineage cells by epidermal growth factor (EGF) to differentiate into odontoblasts. It also stimulates production of alkaline phosphatase and osteocalcin in osteoblasts to improve osteoblast proliferation and differentiation. It also induces osteoclastic activity indirectly by production of IGF-1 and IL- 6 thereby activating RANK-RANKL/OPG phenomenon. It also promotes myofibrillar diameter expansion so it increases muscle mass with strength. Growth Hormone Replacement therapy reduces facial convexity and its main effect appears to be on condylar growth. Since deficiencies of growth hormone can adversely affect orthodontic treatment, effects of growth hormone treatment on craniofacial structures should be considered in order to maximize the effectiveness of orthodontic treatment with appliances and it is more important to understand.