Objective The aim of this study was to evaluate the quality of the most watched YouTube videos regarding the diagnosis and treatment of Carpal Tunnel Syndrome. Methods The first fifty most watched videos for search term “Carpal Tunnel Syndrome” on YouTube, meeting inclusion criteria are included and evaluated by two researchers with DISCERN, JAMA and Carpal Tunnel Syndrome Informational Assessment (CTSIA) scores, under the groups of physicians, health channels and other sources. Results There were statistically significant positive correlation between scoring systems, including CTSIA. No statistically significant correlation was found between video source and number of views, and between video source and view rate. There was no correlation between video source and number of views, and between video source and view rate. There was a relation between video source and DISCERN, JAMA, and CTSIA scores which was statistically significant (p