Determine the prevalence of bruxism in preschool children and identify the main associated factors. A cross-sectional study was conducted involving 151 children aged 3–5 years old. Parents/guardians answered a questionnaire administered in interview form to collect data related to bruxism, parafunctional habits, breastfeeding, sleep and the occurrence of headache. The diagnosis of bruxism was only confirmed when the parent/guardian reported hearing the child grind his or her teeth. Data analysis involved descriptive statistics and Poisson regression (P < 0.05); The prevalence of bruxism among the preschool children was 45.0 % and the following variables were significantly associated with the habit: biting objects (PR = 2.10; 95 % CI: 1.26-3.48), biting one’s cheek (PR =1.76; 95 % CI: 1.22–2.54) and headache located in the temporal region (PR = 1.57; 95 % CI: 1.07–2.32); The prevalence of bruxism was high in the present sample and was associated with the location of headache as well as some parafunctional habits.