Diagnosis Of Alcohol Consumption Research Articles

SERUM DETERMINATION OF MMP-2 AND MMP-9 ACCORDING TO THE PATTERN OF ALCOHOL CONSUMPTION AND IN ALCOHOLIC HEPATIC DISEASE

The damage caused by alcohol consumption generates liver fibrosis, which is characterized by the accumulation of extracellular matrix (ECM); To limit the liver damage, MMP-2 and MMP-9 gelatinases are produced as mediators to degrade ECM products. Their importance in liver damage proposes them as possible markers and target molecules in the diagnosis of alcohol consumption, as well as in alcoholic liver disease [1]. The objective of this work is to evaluate the serum concentrations of MMP-2 and MMP-9 gelatinases in subjects with different patterns of alcohol consumption and in alcoholic liver disease patients. A cross-sectional study was carried out in which subjects with different patterns of alcohol consumption were included. The inclusion was according to the AUDIT, DSM-IV, and clinical and biochemical data of liver disease: risk (Ri), abuse (Ab), dependence (OH), cirrhosis due to alcohol (CiOH) and alcoholic hepatitis (HA). A group without alcohol consumption (TC) was also included for comparison. For the quantification of MMP-2 and MMP-9, a multiple suspension assay (Milliplex®-MERCK ©) was used. Statistical analysis was performed using SPSS V.22 software using Mann Whitney U. P <0.05 was considered statistically significant; values were expressed as mean ± standard error. In 2015 Prystupa, A. et al. used MMP-2 and MMP-9 as markers of progression of damage in alcohol cirrhosis; however, there are no more related studies so far. Our data shows that the synthesis of MMP-2 and MMP-9 in consumption patterns and in liver disease are decreased from risky consumption, promoting the accumulation of ECM in liver tissue. Serum levels of MMP-2 and MMP-9 gelatinases are affected by alcohol consumption, even in a risk pattern. MMP-2 and MMP-9 can be used as markers of alcohol-induced damage in early stages. Conflict of interests: The authors declare that there is no conflict of interest. This work was partially financed by CONACyT SALUD-2016-272579 and PAPIIT- UNAM TA200515

Biochemical markers for alcohol consumption.

A variety of laboratory tests are available to assist in the diagnosis of alcohol consumption and related disorders. The levels of intake at which laboratory results become abnormal vary from person to person. Laboratory tests are particularly useful in settings where cooperativeness is suspected or when a history is not available. Several biochemical and hematological tests, such as γ-glutamyltransferase (GGT) activity, aspartate aminotransferase (AST) activity, high-density lipoprotein cholesterol (HDL-C) content of serum, and erythrocyte mean corpuscular volume (MCV) are established markers of alcohol intake. Their validity as markers is based largely on correlations with recent intake at a single time point and on decreases in elevated values when heavy drinkers abstain from alcohol. These readily available laboratory tests provide important prognostic information and should be integral part of the assessment of persons with hazardous alcohol consumption. There are several other markers with considerable potential for more accurate reflection of recent alcohol intake. These include carbohydrate deficient transferrin, β-hexosaminidase, acetaldehyde adducts and the urinary ratio of serotonin metabolites, 5-hydroxytryptophol and 5-hydroxyindoleacetic acid. These markers provide hope for more sensitive and specific aids to diagnosis and improved monitoring for intake.