SERUM DETERMINATION OF MMP-2 AND MMP-9 ACCORDING TO THE PATTERN OF ALCOHOL CONSUMPTION AND IN ALCOHOLIC HEPATIC DISEASE

Abstract

The damage caused by alcohol consumption generates liver fibrosis, which is characterized by the accumulation of extracellular matrix (ECM); To limit the liver damage, MMP-2 and MMP-9 gelatinases are produced as mediators to degrade ECM products. Their importance in liver damage proposes them as possible markers and target molecules in the diagnosis of alcohol consumption, as well as in alcoholic liver disease [1]. The objective of this work is to evaluate the serum concentrations of MMP-2 and MMP-9 gelatinases in subjects with different patterns of alcohol consumption and in alcoholic liver disease patients. A cross-sectional study was carried out in which subjects with different patterns of alcohol consumption were included. The inclusion was according to the AUDIT, DSM-IV, and clinical and biochemical data of liver disease: risk (Ri), abuse (Ab), dependence (OH), cirrhosis due to alcohol (CiOH) and alcoholic hepatitis (HA). A group without alcohol consumption (TC) was also included for comparison. For the quantification of MMP-2 and MMP-9, a multiple suspension assay (Milliplex®-MERCK ©) was used. Statistical analysis was performed using SPSS V.22 software using Mann Whitney U. P <0.05 was considered statistically significant; values were expressed as mean ± standard error. In 2015 Prystupa, A. et al. used MMP-2 and MMP-9 as markers of progression of damage in alcohol cirrhosis; however, there are no more related studies so far. Our data shows that the synthesis of MMP-2 and MMP-9 in consumption patterns and in liver disease are decreased from risky consumption, promoting the accumulation of ECM in liver tissue. Serum levels of MMP-2 and MMP-9 gelatinases are affected by alcohol consumption, even in a risk pattern. MMP-2 and MMP-9 can be used as markers of alcohol-induced damage in early stages. Conflict of interests: The authors declare that there is no conflict of interest. This work was partially financed by CONACyT SALUD-2016-272579 and PAPIIT- UNAM TA200515