Diabetic Male Rats Research Articles

Sex‐related differences in the visual function of an animal model of type 1 diabetes

Aims/Purpose: Recent basic and clinical research evidence suggests that male–female differences may influence retinal function and structure. It is also well‐established that diabetes affects retinal function. This work aimed to assess whether type 1 diabetes differentially affects neuroretinal function in male and female Wistar rats.Methods: To induce type 1 diabetes, male and female Wistar rats were injected (i.p.) with streptozotocin (65 mg/kg). Electroretinograms (ERGs) were recorded before and 1, 2, and 4 weeks after diabetes induction (n = 12–13 animals/group). To assess the effect of chronic hyperglycemia on the content of molecular components of the blood‐retinal barrier (BRB), specific tight junction proteins were assessed by Western Blotting. Neuroinflammation was evaluated by measuring the retinal levels of pro‐inflammatory cytokines.Results: Some sex‐related changes in retinal function were detected in the diabetic animals. Overall, in male diabetic rats, compared to control male rats, the differences found under scotopic conditions were mainly in the positive scotopic threshold response (pSTR), with decreased amplitudes (at weeks 2 and 4), and higher latencies (at weeks 1 and 4). In contrast, diabetic female rats, compared to control females, presented a higher increase in the latency value of the negative STR (nSTR; at weeks 1 and 4), a‐wave (at weeks 1, 2, and 4), and oscillatory potentials 2, 3, and 4 (at weeks 1, 2, and 4). Under photopic conditions, male diabetic rats showed higher implicit time values of the fundamental flicker wave (at week 4) and a decrease in the amplitude of the flicker 2nd harmonic (at week 4) compared to control males. Conversely, in diabetic female rats, there were no differences in these parameters compared to control female rats. Concerning the BRB and neuroinflammation, no changes were found in diabetic males and females compared to their respective controls.Conclusions: Our findings suggest that type 1 diabetes differentially affects the retinal function in male and female Wistar rats.Support: FCT‐Portugal: PEst UIDB/04539/2020 and UIDP/04539/2020; 2020.07432.BD.

Impact of Baobab Consumption on Some Biochemical Alterations in Male Diabetic Rats

Impact of Baobab Consumption on Some Biochemical Alterations in Male Diabetic Rats

Hypoglycemic And Hypolipidemic Biochemical Effects of Bee Venom on Experimentally Induced Diabetes in Rats

Abstract Background:‎ Diabetes mellitus (DM) is the highest prevalent endocrine illness that exhibits hyperglycemia, ‎hyperlipidemia and protein metabolism disorders also elevated risk of several complications, including ‎vascular disorders. A variety of polypeptide agents can be found in bee venom. The two main ingredients ‎of bee venom are phospholipase A2 and mellitin. These constituents enhance the release of insulin from ‎the Beta islands of pancreas. The purpose of the current work was to demonstrate the hypoglycemic and ‎hypolipidemic effects of bee venom on male diabetic rats that induced by alloxan. ‎ Methods:‎ ‎ The experimental investigation was carried out on 40 male rats (weight from180 to 220 g) which were ‎assigned to four groups (10/each): (the first) a negative control group (not receiving therapy), (the ‎second) a positive control group (diabetic group) injected with alloxan only (160 mg/kg), (the third) ‎diabetic group administered with Vildagliptin (6mg/kg) and the forth diabetic group treated with Bee ‎venom (0.5mg/kg). After two months of the investigation, blood samples were assembled. ‎ Results:‎ Bee Venom (0.5mg/kg) lowered significantly (p≤0.05) the level of serum glucose, which was followed ‎by a substantial rise in the level of HDL and insulin when contrasted to the rats with diabetes. Total lipid, ‎cholesterol, triacylglycerol and LDL levels were considerably reduced in the diabetic group that received ‎BV.‎ Conclusion:‎ There is a growing interest in the bee venom's use as a natural product rather than chemical drugs for ‎treatment of diabetes mellitus because of the manner it affects rats biochemically, particularly its ‎prospective anti-diabetic and anti-hyperlipidemia qualities.‎

Evaluation of the anti-diabetic effects of Tapinanthus preussii (African mistletoe) aqueous leaf extract in streptozotocin induced diabetic male wistar albino rats

Evaluation of the anti-diabetic effects of Tapinanthus preussii (African mistletoe) aqueous leaf extract in streptozotocin induced diabetic male wistar albino rats

The Effect of Probiotic Supplementation on the Levels of Biomarkers in Diabetic Animal Model: A Research Protocol

Introduction: Type 2 Diabetes Mellitus (T2DM) is characterised by hyperglycaemia, insulin secretion defects, or resistance. Most T2DM drugs help to improve glycaemic status, but the response varies among individuals. The modern lifestyle with unhealthy eating habits leads to gut dysbiosis. Altered gut microbiota can disrupt the host’s metabolic and signaling pathways, intestinal barrier integrity, and function. Probiotics could restore a healthy microbiota in the intestine, thus improving glycaemic status. Need of the study: Probiotics may assist in re-establishing a healthy microbiota composition in the intestine. Limited studies have evaluated the supplementation of probiotics for effectively managing T2DM. Aim: To investigate the effects of probiotics on biomarker levels in type 2 diabetic male Wistar rats. Materials and Methods: This interventional case-control study will be conducted at the Centre for Toxicology and Developmental Research (CEFTE) at Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India. The study will involve 46 male Wistar rats divided into five groups. Diabetes will be induced by feeding the animals a High-Fat Diet (HFD) and administering a low dose of Streptozotocin (STZ) injection. Groups 1 and 2 will be on basal and HFDs, serving as negative and positive controls, respectively. Groups 3, 4 and 5 will be the intervention groups. The study duration will be four weeks and three days for diabetes induction and six weeks for intervention. Blood samples will be collected periodically to assess biomarkers, and at the end of the study, internal organs will be harvested for histopathological examination. Institutional Ethics Committee (IEC) approval has been obtained. Categorical variables will be analysed using Chi-square or Fisher’s exact test, while continuous variables will be analysed using repeated measures Analysis of Variance (ANOVA). A p-value of ≤0.05 will be considered statistically significant, and statistical analysis will be performed using Statistical Package for Social Sciences (SPSS) version 16.0.

Duodenal-Jejunal Bypass Restores Sweet Taste Receptor-Mediated Glucose Sensing and Absorption in Diabetic Rats.

Aim: The aim of the study is to identify the regulatory role of intestinal sweet taste receptors (STRs) and glucose transporters (SGLT1, GLUT2) and gut peptide secretion in duodenal-jejunal bypass (DJB)-ameliorated glycemic control in Type 2 diabetes. Materials and Methods: DJB and sham surgeries were performed in streptozotocin-induced diabetic male rats. The blood GLP-1 and GLP-2 levels were evaluated under feeding and fasting conditions. The expression of STRs (T1R2, T1R3), sweet taste signaling effector (Gα-gustducin), SGLT1, and GLUT2 was detected in the intestinal alimentary limb (A limb), biliopancreatic limb (BP limb), and common limb (C limb). The effects of STR inhibition on glucose control were measured with lactisole. Results: Glucose tolerance was improved in DJB-operated rats compared with the sham group, similar to that of normal control rats, without significant differences in food intake and body weight. The plasma GLP-1 levels of DJB rats were increased under diet-fed condition, and GLP-2 levels were increased after fasting. The villus height and crypt depth were significantly increased in the A limb of DJB-operated rats. In addition, GLP-1 expression was restored in enterocytes. The expression of T1R2, Gα-gustducin, and SGLT1 was elevated in the A limb after DJB, while GLUT2 was downregulated in the A, BP, and C limbs. The localization of GLUT2 was normalized in the three intestinal limbs after DJB. However, the beneficial effects of DJB on glucose control were abolished in the presence of lactisole in vivo. Conclusion: DJB ameliorates glycemic control probably by restoring STR-mediated glucose sensing and absorption with the responses of GLP-1 and GLP-2 to carbohydrate.

Hyperglycemic and oxidative stress management efficacies of hydro-methanol extract of Ipomoea mauritiana (Jacq.) root tuber in streptozotocin (STZ) induced diabetic male rats: Dose selection study

Hyperglycemic and oxidative stress management efficacies of hydro-methanol extract of Ipomoea mauritiana (Jacq.) root tuber in streptozotocin (STZ) induced diabetic male rats: Dose selection study

Evaluation of Technological and Antihyperglycemic Effects of Pan Bread Enriched with Okra Pod Waste on Diabetic Male Rats

Evaluation of Technological and Antihyperglycemic Effects of Pan Bread Enriched with Okra Pod Waste on Diabetic Male Rats

Role of serotonin on pituitary-thyroid functions and antioxidants in diabetic male rats

Diabetes mellitus causes an increase in oxidative stress. Serotonin is a hormone its level in circulation reduced in diabetes; recently, it has been found to possess antioxidant qualities. The purpose of the study was to look at the impact of diabetes on pituitary-thyroid functioning, and serotonin role in modulating these effects in a streptozotocin-induced diabetes mellitus rat model. The thirty-tow healthy male rats with a body weight of 180-200 gm were split equally into 4 groups, group [1[: control, group [2]: Serotonin, group [3]: diabetic, and group [4]: Serotonin-diabetic. The rats were weighed and serum levels of glucose, insulin, malondialdehyde, superoxide dismutase, catalase, glutathione peroxidase, serotonin, thyroid stimulating hormone, thyroxine, and triiodothyronine were measured. Our results presented that there was a significant increase in T3, T4, glucose, and MDA in the diabetic group compared to the control, while a significant decrease in T3, and T4, glucose, and MDA in the Serotonin with diabetic group compared to diabetic group. Furthermore, a significant decrease in TSH, serotonin, insulin and GPX, CAT, and SOD antioxidants in the diabetic group compared to control, while a significant increase in serum TSH, serotonin, insulin and CAT, GPX and SOD antioxidants in the Serotonin+diabetic group compared to diabetic group as well as a significant increase in serum insulin in the Serotonin group compared to all other groups. Serotonin plays a protective role against diabetes-induced and pituitary-thyroid disorder caused by diabetes which might be because of its antioxidant qualities and ability to maintain glucose and insulin balance.

Possible Effects of Dinitrophenol and Caffeine on Diabetic Adult Male Albino Rat

Possible Effects of Dinitrophenol and Caffeine on Diabetic Adult Male Albino Rat

Evaluation of Biochemical Changes in Induced Diabetic Male Rats by Different Methods.

Evaluation of Biochemical Changes in Induced Diabetic Male Rats by Different Methods.

Antioxidant activities of aqueous leaf extract of Parquetina nigrescens in high-fat-diet-streptozotocin-induced diabetic male albino rats

Antioxidant activities of aqueous leaf extract of Parquetina nigrescens in high-fat-diet-streptozotocin-induced diabetic male albino rats

Investigating the Effects of 8 Weeks of Resistance Training on the Levels of P53 and Nrf2 Proteins in Kidney Tissue in Diabetic Rats with Morphine Withdrawal Syndrome

Introduction: The effect of exercise training on kidney tissue in the condition of diabetes with morphine withdrawal syndrome is unclear. The aim of this study was to investigate the effect of 8 weeks of resistance training on the levels of P53 and Nrf2 proteins in kidney tissue in diabetic male rats with morphine withdrawal syndrome.
 Methods: This experimental study utilized a sample of 32 Wistar rats. Following the induction of diabetes, the Wistar rats were randomly divided into four groups of eight based on their weight: diabetes, morphine diabetes, diabetes with resistance training, and morphine diabetes with resistance training. The oral technique was employed for a duration of 21 days to establish a reliance on morphine.. The resistance training protocol was implemented for 8 weeks. At the end, all mice were anesthetized, killed and their tissue was extracted. The variable protein levels of this study were measured by ELISA kits. Data were analyzed using one-way analysis of variance and Tukey's post hoc test with the help of SPSS version 16 software at a significance level of p ≤ 0.05.
 Results: Resistance training caused a significant decrease in P53 in the groups of diabetes + resistance training (P=0.006) compared to diabetes and morphine diabetes + resistance training (P=0.012) compared to morphine diabetes group. Similarly, a significant decrease in Nrf2 was observed in the morphine diabetes + resistance training group (P=0.013) compared to the morphine diabetes group.
 Conclusion: Resistance training probably reduced kidney tissue damage in diabetic and diabetic rats with withdrawal syndrome by reducing P53 protein, which indicates the protective effect of resistance training on kidney tissue in pathological conditions.

In diabetic male Wistar rats, quercetin-conjugated superparamagnetic iron oxide nanoparticles have an effect on the SIRT1/p66Shc-mediated pathway related to cognitive impairment

BackgroundQuercetin (QC) possesses a variety of health-promoting effects in pure and in conjugation with nanoparticles. Since the mRNA-SIRT1/p66Shc pathway and microRNAs (miRNAs) are implicated in the oxidative process, we aimed to compare the effects of QC and QC-conjugated superparamagnetic iron oxide nanoparticles (QCSPIONs) on this pathway.MethodsThrough the use of the chemical coprecipitation technique (CPT), SPIONs were synthesized, coated with dextran, and conjugated with quercetin. Adult male Wistar rats were given intraperitoneal injections of streptozotocin to look for signs of type 1 diabetes (T1D). The animals were randomized into five groups: the control group got deionized water (DI), free QC solution (25 mg/kg), SPIONs (25 mg/kg), and QCSPIONs (25 mg/kg), and all groups received repeat doses administered orally over 35 days. Real-time quantitative PCR was used to assess the levels of miR-34a, let-7a-p5, SIRT1, p66Shc, CASP3, and PARP1 expression in the hippocampus of diabetic rats.ResultsIn silico investigations identified p66Shc, CASP3, and PARP1 as targets of let-7a-5p and miR-34a as possible regulators of SIRT1 genes. The outcomes demonstrated that diabetes elevated miR-34a, p66Shc, CASP3, and PARP1 and downregulated let-7a-5p and SIRT1 expression. In contrast to the diabetic group, QCSPIONs boosted let-7a-5p expression levels and consequently lowered p66Shc, CASP3, and PARP1 expression levels. QCSPIONs also reduced miR-34a expression, which led to an upsurge in SIRT1 expression.ConclusionOur results suggest that QCSPIONs can regulate the SIRT1/p66Shc-mediated signaling pathway and can be considered a promising candidate for ameliorating the complications of diabetes.

Effects of Celery Leaf Aqueous Extract on Fertility and Liver Enzyme in Diabetic Male Rats

Effects of Celery Leaf Aqueous Extract on Fertility and Liver Enzyme in Diabetic Male Rats

Effect of Hibiscus rosa-sinensis and Camel Milk on the Reproduction of Diabetic Male Albino Rats – A Review

Diabetes mellitus is a long-term medical condition identified by high blood sugar levels. Two forms of diabetes most commonly occur. Type I diabetes occurs in children and type II diabetes is normally found in adults. Although diabetes affects the whole body's function surprisingly, it gives rise to male infertility. Diabetes causes impotency, and ejaculatory disorders and decreases libido in males. Hibiscus rosa-sinensis and camel milk both are known for their anti-diabetic properties. Both are able to deal with the oxidative stress caused by diabetes because of their antioxidant properties. Both have other properties like antibacterial, anti-inflammatory, antifungal, antimicrobial, antitumor, cardio-protective, hepato-protective etc. The crude extract of Hibiscus rosa-sinensis has insulin-secreting activity, significantly lowering the glucose level of blood in diabetic rats. Camel milk contains insulin-like protein which is also reported to positively decrease the blood sugar level.

Mimosa pudica Linn. extract improves aphrodisiac performance in diabetes-induced male Wister rats

Male sexual dysfunction is considered one of the major consequences of diabetes mellitus. The medicinal plant, Mimosa pudica Linn. is believed to have numerous therapeutic effects, including anti-diabetic, anti-obesity, aphrodisiac, and a sexual behaviour-enhancing properties. In the present study, the significant effect of ethanolic extract of M. pudica L. to scavenge excessive free radicals and alleviate the deleterious effects of alloxan-induced diabetes on the male sexual system of rats was demonstrated. The rats treated with the M. pudica L. extract recovered their body weight, the weight of their reproductive organs, the characteristics of the sperm and the histocellular arrangement of the testes. In addition, significant levels of hormones (testosterone, follicle-stimulating hormone and luteinising hormone) increased in both serum and testicular homogenates of male diabetic rats treated with M. pudica L. extract. Further, antioxidant enzymes, SOD, CAT, GSH, and GPx levels are increased, and oxidative stress markers MDA and ROS are reduced in both serum and testicular homogenates of M. pudica L. extract treated male rats. Furthermore, an in silico molecular docking study was performed to predict high potential compounds of M. pudica L. extract against the PDE5 receptor. Two bioactive compounds, namely 3-Dibenzofuranamine (-11.1 kcal × mol−1), Stigmasta-7,16-dien-3-ol (-10.4 kcal × mol−1) showed the highest binding affinities with PDE5 enzyme, much higher than the reference drug sildenafil (-9.9 kcal × mol−1). According to these findings, bioactive compounds rich in ethanolic extract of M. pudica L. have significant aphrodisiac performance in diabetic rats. Communicated by Ramaswamy H. Sarma

Evaluation The Ameliorative Effect of Alcoholic Extracts of Citrullus colocynthis and Phyllanthus Emblica Fruits on Streptozotocin-Induced Diabetes in Male Rats

Evaluation The Ameliorative Effect of Alcoholic Extracts of Citrullus colocynthis and Phyllanthus Emblica Fruits on Streptozotocin-Induced Diabetes in Male Rats

The impact of Jussiaea repens aqueous extract on Hyperglycemic and Hematological markers in Diabetic male rat induced by Streptozotocin

In diabetic rats, the hypoglycemic potential of an aqueous extract of Jussiaea repens (50mg/100gm body weight) was examined. The herbal extract's impact on a number of hematological indicators was also studied. Eighteen albino rats, each contain 6 rats, were placed into three groups: vehicle control, diabetes control, and supplemented group. To develop diabetes, a single i.p injection of STZ (80mg/kg body weight) was given. Hematological markers include hemoglobin (Hb), red blood cell (RBC) count, white blood cell (WBC) count, packed cell volume (PCV), lymphocytes, and neutrophils, as well as blood glucose levels and HbA1c were assessed. STZ-induced diabetic rats fed the extract had lower fasting blood glucose levels, which was statistically significant (p<0.05). The HbA1c level in the diabetic control group increased drastically, but the level in the extract-treated supplemented group reduced significantly. Hb, PCV, RBC, and WBC as well as their differentials, were significantly reduced in diabetic control rats, whereas the supplemented group recovered significantly.. These findings imply that the aqueous extract of Jussiaea repens has hypoglycemic action and overcomes the abnormalities in STZ-induced diabetic rats' blood parameters.

Protective Effects of Rheum Ribes Hydroalcoholic Extract on Blood Glucose and Renal Histological Structure in Streptozotocin-Induced Diabetic Male Rats

Protective Effects of Rheum Ribes Hydroalcoholic Extract on Blood Glucose and Renal Histological Structure in Streptozotocin-Induced Diabetic Male Rats