DI Groups Research Articles

Effect of information provision on parental intention toward COVID-19 vaccination for children: a nationwide survey experiment

The reluctance of parents to vaccinate their children against COVID-19 was prevalent particularly when uncertainty over vaccination outcomes prevailed. We conducted a nationwide randomized online survey experiment to assess the effect of information provision on parental intention for COVID-19 vaccination before the government started vaccination for children in South Korea. Parents of elementary school children were provided with either no information (Control), information on vaccine profile (vaccine informed group; VI), or COVID-19 (disease informed group; DI). Among 359,110 participants, parental intention for vaccination of children was significantly higher in both VI and DI groups compared with the Control group. In terms of effect size, information on COVID-19 vaccine increased likelihood to vaccinate by 1620 per 100,000 parents and reduced vaccine hesitancy by 1340 per 100,000 parents. Consistently with the positive effect on vaccination intention, both VI and DI interventions increased participants’ perceptions on vaccination benefits being higher than its risks and vaccination risks being lower than health risks of COVID-19 infection, and self-reported trust in COVID-19 information. Our results lend strong support to the claim that the provision of targeted, tailored information on COVID-19 vaccine and infection increases parental intention to vaccinate children and reduces vaccine hesitancy.

Time-dependent progress of lower urinary tract dysfunction in streptozotocin-induced diabetic rats with or without low-dose insulin treatment.

Objectives: To examine time-dependent functional and structural changes of the lower urinary tract in streptozotocin-induced diabetic rats with or without low-dose insulin treatment and explore the pathophysiological characteristics of insulin therapy on lower urinary tract dysfunction (LUTD) caused by diabetes mellitus (DM). Methods: Female Sprague-Dawley rats were divided into five groups: normal control (NC) group, 4 weeks insulin-treated DM (4-DI) group, 4 weeks DM (4-DM) group, 8 weeks insulin-treated DM (8-DI) group and 8 weeks DM (8-DM) group. DM was initially induced by i.p. injection of streptozotocin (65 mg/kg), and then the DI groups received subcutaneous implantation of insulin pellets under the mid dorsal skin. Voiding behavior was evaluated in metabolic cages. The function of bladder and urethra in vivo were evaluated by simultaneous recordings of the cystometrogram and urethral perfusion pressure (UPP) under urethane anesthesia. The function of bladder and urethra in vitro were tested by organ bath techniques. The morphologic changes of the bladder and urethra were investigated using Hematoxylin-Eosin and Masson's staining. Results: Both 4-and 8-weeks diabetic rats have altered micturition patterns, including increased 12-h urine volume, urinary frequency/12 hours and voided volume. In-vivo urodynamics showed the EUS bursting activity duration is longer in 4-DM group and shorter in 8-DM group compared to NC group. UPP change in 8-DM were significantly lower than NC group. While none of these changes were found between DI and NC groups. Organ bath showed the response to Carbachol and EFS in bladder smooth muscle per tissue weights was decreased significantly in 4- and 8-weeks DM groups compared with insulin-treated DM or NC groups. In contrast, the contraction of urethral muscle and maximum urethral muscle contraction per gram of the tissue to EFS stimulation were significantly increased in 4- and 8-weeks DM groups. The thickness of bladder smooth muscle was time-dependently increased, but the thickness of the urethral muscle had no difference. Conclusions: DM-induced LUTD is characterized by time-dependent functional and structural remodeling in the bladder and urethra, which shows the hypertrophy of the bladder smooth muscle, reduced urethral smooth muscle relaxation and EUS dysfunction. Low-dose insulin can protect against diuresis-induced bladder over-distention, preserve urethral relaxation and protect EUS bursting activity, which would be helpful to study the slow-onset, time-dependent progress of DM-induced LUTD.

Intradermal Sterile Water Injection: Safe and Effective Alternative for Relief of Acute Renal Colic in the Emergency Department

BackgroundThe optimal pain relief method for acute renal colic in the emergency department remains controversial. ObjectiveWe compared the safety and efficacy of intradermal sterile water injection (ISWI) to treatment with intramuscular (IM) diclofenac, intravenous (IV) opioids, and IV paracetamol in patients with acute renal colic. MethodsThis randomized, single-blind study included 320 patients with renal colic to one of four treatment groups. The first group received ISWI at four different points around the most painful flank area. Patients in the DI, PARA, and TRAM groups received 75 mg IM diclofenac, 1 g IV paracetamol, and 100 mg IV tramadol, respectively. Pain intensity was measured using a visual analog scale (VAS) before treatment and 15, 30, and 60 min after treatment. ResultsVAS scores 15 and 30 min after treatment were significantly lower in group ISWI than in groups DI, PARA, and TRAM. However, there were no significant differences in the decrease in the pain score at baseline and at 60 min after treatment. In addition, fewer patients required rescue analgesia in group ISWI than in group TRAM. However, no significant differences were observed between group ISWI and group DI or PARA in terms of the need for rescue analgesia. Finally, there were significantly fewer adverse events in group ISWI than in groups DI and TRAM. ConclusionsISWI had similar efficacy, faster pain relief, and lower need for rescue analgesia compared with diclofenac, paracetamol, and tramadol for the management of acute renal colic. In addition, ISWI was well-tolerated and had no adverse effects.

Preliminary Evidence That Fiji Water Has Protective Effects against Aluminum Toxicity in Honey Bees (Apis mellifera).

Researchers have determined that bioavailable aluminum chloride (AlCl3) may affect honey bee behavior (e.g., foraging patterns and locomotion) and physiology (e.g., abdominal spasms). The purpose of these experiments was to determine if Fiji water reduces the impacts of AlCl3 toxicity in bees by measuring circadian rhythmicity (number of times bees crossed the centerline during the day and night), average daily activity (average number of times bees crossed the centerline per day), and mortality rates (average number of days survived) using an automated monitor apparatus. Overall, the AlCl3 before and after Fiji groups had significantly higher average daily activity and rhythmicity rates compared to their respective AlCl3 before and after deionized water (DI) groups. One of the AlCl3 before DI groups exhibited no difference in rhythmicity rates compared to its respective AlCl3 after Fiji group. Overall, these results suggest that Fiji water might exert protective effects against AlCl3. The AlCl3 groups paired with Fiji water had higher activity and rhythmicity levels compared to the AlCl3 groups paired with DI. It is important for researchers to continue to study aluminum and possible preventatives for aluminum uptake.

Perbandingan Antara Perkembangan Kinerja Ekonomi Daerah Otonomi Baru dan Daerah Induk di Indonesia Pasca Pemekaran

This study aims to compare the development of economic performance between the parent region and the new autonomous regions after the division. The research sample consists of 27 regions, including 14 new autonomous regions (DOB) and 13 parent regions (DI) which are affected areas from the expansion carried out during the period 2012 and 2013. The research sample uses data from 2014-2018, which is data from the first five year available after the expansion. The research uses the non-parametric Mann-Whitney test by comparing the mean rank values between the DOB and DI groups annually through the t-test. As a result, it is known that the new autonomous regions have developed a level of regional financial capacity, a level of regional independence, and a level of dependence on transfer funds that are better than the parent region. On the other hand, the growth of GRDP per capita and the poverty rate in the new autonomous regions show worse performance than the parent regions.

Effects of Postdischarge High-Protein Oral Nutritional Supplements and Resistance Training in Malnourished Surgical Patients: A Pilot Randomized Controlled Trial.

The presence of malnutrition is increasingly becoming a postdischarge problem in surgical patients. We aimed to investigate whether oral nutritional supplements combined with resistance training could minimize skeletal muscle atrophy in surgical patients after discharge. This randomized controlled study was conducted at the Department of Surgery, National Hospital of Faroe Islands from 2018 to 2020. A total of 45 patients aged 37–74 years participated and were allocated to one of three groups: diet (DI; n = 13), exercise and diet (EX + DI; n = 16), or control (CON; n = 16). The intervention period lasted 8 weeks. The intervention groups received individual dietary counselling and a protein-rich oral nutritional supplement twice a day containing 22 g of protein/day. Patients in the EX + DI group were assigned to resistance training sessions. Patients in the CON group received standard care. The primary outcome was change in lean body mass (LBM). Secondary outcomes were change in body weight, handgrip strength, quality of life, surgery-related side effects, energy and protein intake, length of stay and one-year mortality. To estimate within-group changes, linear mixed models including group–time interactions as fixed effects and patients as random effects were fitted. Within-group change in LBM was 233, 813 and 78 g in the DI, EX + DI and CON groups, respectively, with no significant between-group difference (p > 0.05). Pain score declined more (p = 0.04) in the EX + DI group compared with the CON group. Body weight, handgrip strength, quality of life and surgery-related side effects did not differ between groups. At the end of study, mean cumulative weight change in the DI and EX + DI groups was 0.4% and 1.6%, respectively, whereas the CON group experienced a weight loss of −0.6%. No significant difference in primary outcome between groups was noted. However, our results indicate some benefits from exercise and nutrition for malnourished surgical patients.

Longitudinal In Vitro Effects of Silver Diamine Fluoride on Early Enamel Caries Lesions.

This laboratory study evaluated the longitudinal surface microhardness changes in early, incipient, noncavitated, white-spot, enamel caries lesions treated with silver diamine fluoride (SDF). Five intervention groups (SDF, AgNO3, KF, 5% sodium fluoride varnish (FV), deionized water (DI)) × two-time intervals after intervention (immediate & delayed pH-cycling) resulted in 10 groups (n=18). Silver nitrate (AgNO3) and potassium fluoride (KF) groups served as controls to assist in evaluating if remineralization effects were due to the silver or fluoride component in SDF. Early, incipient, noncavitated, white-spot, enamel caries lesions were created in bovine enamel, the extent of demineralization was determined using Vickers surface microhardness (VHNlesion). Intervention treatments were applied. Half the specimens from each group underwent immediate 5-day pH-cycling, and half were stored in an incubator with artificial saliva for 2 weeks before undergoing 5-day pH-cycling. After pH-cycling, lesion hardness was evaluated using VHNpost. Specimens were then exposed to a second demineralization challenge, and lesion softening was evaluated (VHNsecdem). Hardness variables were calculated: ΔVHN = VHNpost - VHNlesion; ΔVHNsecdem = VHNsecdem - VHNpost. Data were analyzed using two-way ANOVA (α=0.05). Immediately cycled, SDF had significantly (p<0.0001) greater remineralization than DI, AgNO3, and FV. All delayed cycling groups had significantly greater remineralization than FV (p<0.0001). Significantly greater remineralization was noted in delayed AgNO3 (p≤0.0001), DI (p=0.0003), and FV (p=0.0006) compared to immediately cycled. After the second demineralization challenge, FV had significantly less surface softening than AgNO3 (p=0.0002), DI (p=0.0003), KF (p=0.0225), and SDF (p=0.0388) intervention groups. No significant difference was found between the pH-cycle timings (p=0.2710). Based the present findings, FV may be better suited than SDF to treat early, incipient, noncavitated, white-spot, enamel caries lesions.

RIP1 Regulates Mitochondrial Fission during Skeletal Muscle Ischemia Reperfusion Injury

Background Dynamin related protein-1 (Drp1)-mediated mitochondrial fission relates to ischemia reperfusion (IR) injury, and its association with necroptosis is implied. We hypothesized that receptor-interacting protein 1 (RIP1), a key kinase in necroptosis, acted as an upstream of Drp1-mediated mitochondrial fission during skeletal muscle IR. Methods Thirty rats were randomized into the SM, IR, NI, MI, and DI group (n = 6). The rats in the SM group were shamly operated, and those in the IR group were subjected to 4-hour ischemia of the right hindlimb that was followed by 4-hour reperfusion. Intraperitoneal administration of Nec-1 1 mg/kg, Mdivi-1 1.2 mg/kg and same volume of DMSO were given before ischemia in the NI, MI and DI groups, respectively. Upon reperfusion, the soleus muscles were harvested to determine morphological changes and the expression of RIP1, total Drp1 and p-Drp1 (Ser616). Moreover, the muscular oxidative stress indicators and plasma muscle damage biomarkers were detected. Results IR led to impaired histopathological structures and mitochondrial fragmentation in the soleus muscle tissue, accompanied with increased muscular oxidative stress and muscle injury biomarkers, which could be similarly alleviated by Mdivi-1 and Nec-1 (p < 0.05). RIP1 and p-Drp1 (Ser616) protein levels were significantly upregulated in the soleus muscle subjected to IR injury, this upregulation was attenuated in the NI group, and Mdivi-1 downregulated the protein expression of p-Drp1 (Ser616) but not of RIP1 (p < 0.05). Conclusion RIP1 functions as an upstream of Drp1-mediated mitochondrial fission in the execution of necroptosis during skeletal muscle IR.

Novel urea‐based compounds as solid‐solid phase change materials: 1,3‐bisstearoylurea and 1,1,3,3‐tetrastearoylurea for thermal energy storage applications

1,3-Bisstearoylurea and 1,1,3,3-tetrastearoylurea were produced as solid-solid phase change materials (SS-PCMs) for potential solar thermal energy storage (TES) applications via condensation of urea with the respective amount of carboxyl chloride (stearoyl chloride). Both 1,3-bisstearoylurea and 1,1,3,3-tetrastearoylurea were characterized by Fourier-transform infrared (FT-IR), 1H nuclear magnetic resonance, differential scanning calorimetry (DSC), and thermogravimetric (TG) analysis techniques. 1,3-Bisstearoylurea and 1,1,3,3-tetrastearoylurea had considerable melting/crystallization enthalpy. The reason for crystallization was structural symmetry and flexibility of long alkyl groups there. Di and tetra alkyl groups made it possible to discuss the structure-property relationship for organic PCMs. Besides, the compounds were characterized through DSC and FT-IR spectroscopy before and after thermal cycling to determine their thermal reliability. Solid-solid phase change enthalpies of them for melting and crystallization were found 95.86 and −87.6 Jg−1 for 1,3-bisstearoylurea, respectively and 27.7 and −25.8 Jg−1 for 1,1,3.3-tetrastearoylurea, respectively. Thermal conductivity was studied to increase by simply expanded graphite addition. The thermal endurance limits of 1,3-bisstearoylurea and 1,1,3,3-tetrastearoylurea compounds were studied using TG analysis.

Diagnostic Efficacy and Clinical Value of Ultrasonography in Difficult Airway Assessment: Based on a Prospective Cohort Study.

Diagnostic Efficacy and Clinical Value of Ultrasonography in Difficult Airway Assessment: Based on a Prospective Cohort Study.

Dyslipidaemia of Alloxan-Induced Type 1 Diabetes Mellitus in Dogs is attenuated by Ethanolic Extracts of Anogeissus Leiocarpus Stem Bark

Introduction: The effect of ethanolic extract of Anogeissus leiocarpus stem bark on dyslipidaemia induced by diabetes mellitus (DM) was investigated, since enhanced pro-synthetic hypoglycaemic drugs did not effectively control dyslipidemia of DM, apart from causing toxicity and resistance. Materials and Methods: Twelve adult Nigerian indigenous dogs were placed in 4 groups of 3 each, namely non diabetic (ND); diabetic untreated (DU); diabetic treated with insulin (DI) and diabetic treated with extract (DE). Body weights and lipid profiles were measured during a four-week period. Results: Significant percentage body weight decreased in DU (50.21 %) throughout the period of study with continuous increase in body weight in ND (46.96 %). In DI and DE there were initial decreases in body weights (11.08% and 14.54% respectively) which significantly increased (23.53 % and 32.30 % respectively) by the fourth week. Lipid profiles revealed significant (P &lt; 0.002) increases in triglycerides, total cholesterol and low-density lipoproteins (LDL) in DU group when compared with the DE group. There was also a significant (P &lt; 0.002) decrease in high density lipoprotein (HDL) in the DU when compared to DE, DI and ND groups. Conclusion: The ethanolic extract of Anogeissus leiocarpus stem bark attenuated the dyslipidaemia produced in alloxaninduced type 1 diabetes mellitus in Nigerian indigenous dogs.

Comparison between stereophotogrammetric, digital, and conventional impression techniques in implant-supported fixed complete arch prostheses: An in vitro study

Statement of problemConventional impressions and digital intraoral scanning for implant-supported fixed complete arch prostheses still have many problems that influence accuracy. Although stereophotogrammetry may offer a reliable alternative to other techniques, it has seldom been investigated. PurposeThe purpose of this in vitro study was to measure and compare the intraoral scan body deviations of the reference cast with the intraoral scan body distortions obtained by conventional, digital, and stereophotogrammetric techniques. Materials and methodsAn edentulous maxillary “all-on-four” cast was prepared with 2 straight and two 17-degree angled screw-retained abutments screwed on the implant. Three capture techniques were compared: the conventional impression technique (CI group) using impression plaster (IP), the digital intraoral scanning (DIS group) technique, and the stereophotogrammetry (SPG group) technique. A calibrated extraoral scanner was used to digitize the definitive cast to compare its intraoral scan body positions with those of the other techniques in terms of global angular distortion and 3D deviations of the whole scan body and flat angled surface alone by using an inspection and metrology software program and the best fit alignment technique. The Kolmogorov-Smirnov and Shapiro-Wilk tests showed normal distribution of the quantitative variables. Thus, the repeated measures analyses of variance followed by univariate analysis and Bonferroni multiple comparison tests were performed to analyze the data (α=.05). ResultsSignificant global angular discrepancies and 3D deviations of the whole scan body and flat angled surface were found among the CI, DI, and SPG groups for both trueness (P<.001) and precision (P<.001). ConclusionsThe stereophotogrammetry capture technique reported the highest accuracy in terms of trueness and precision for the intraoral scan bodies of all the techniques evaluated. However, at the flat angled surface region of the scan body, higher trueness was detected with the digital technique. Conventional impressions showed better trueness results than the digital ones, but the opposite was true of precision.

The Influence of Dissonance Induction and Assessment Reactivity in Improving Adherence to COVID-19 Precautionary Measures: A Cluster Randomised Controlled Trial

BackgroundDuring the coronavirus disease 2019 (COVID-19) pandemic, adherence to suggested precautionary measures has been emphasized as important in preventing and curtailing its spread. However, strict adherence to precautionary measures can be demanding. MethodsThis cluster randomised controlled trial done among 1517 undergraduate dental students tested the effectiveness of ‘dissonance induction’ (DI) and ‘assessment reactivity’ (AR) in improving adherence to World Health Organization (WHO) measures as compared to a control group. At baseline, participants in the DI group were tested for their knowledge of precautionary measures, immediately followed by assessment of their adherence to them. This methodology was adapted to systematically reveal the poor adherence of the participants in their self-held cognitions, should there be any. The magnitude of dissonance was measured as the proportion of such dissonant cognitions held by an individual. In the AR group, at baseline, participants were asked about their attitudes alone toward measures. The control group was neither assessed for knowledge and adherence nor for attitudes toward the measures at baseline. Two weeks after the administration of these interventions in the DI and AR groups, the 3 study groups were assessed for adherence. ResultsThe follow-up adherence scores in the DI group were found to be significantly higher (15.11 ± 4.1) compared to the AR (13.13 ± 2.01) and control (12.87 ± 2.97) groups as analysed by Kruskal-Wallis analysis of variance (H = 243.5; P < .001). Wilcoxon signed-rank test showed that the adherence scores significantly improved in the DI group from baseline to follow-up (z = -8.84; P < .001). Magnitude of dissonance at baseline was found to be a significant predictor of follow-up adherence scores (R2 = 0.255). ConclusionThis study found that DI is an easy intervention to bring an immediate and significant change in adherence to precautionary measures.

Effects of increased physical activity and/or weight loss diet on serum myokine and adipokine levels in overweight adults with impaired glucose metabolism.

The purpose of this study was to investigate the changes in serum irisin, fibroblast growth factor-21 (FGF21), visfatin, follistatin like protein-1 (FSTL1), and meteorin-like protein (Metrnl) levels in response to increased physical activity and/or diet interventions in overweight subjects with impaired glucose metabolism (IGM). A total of 60 subjects (BMI > 25.0 kg/m2) with IGM were recruited in this single-centered interventional study. Twelve subjects dropped out during the study and the study was completed with 48 patients. Patients were divided into two groups as diet only (DI, n = 24) and diet and physical activity intervention (DPA, n = 24). Patients in DI group received a diet program while DPA group received a diet combined with a physical activity intervention for 12 weeks. Additional 24 healthy subjects were recruited to compare the baseline levels of proteins. Serum protein levels, anthropometric measurements, and biochemical parameters were assessed. Irisin, FGF21, visfatin, and FSTL1 levels significantly decreased in both groups after 12-week intervention (p < 0.001). However, there were no differences in protein levels between DI and DPA groups (p > 0.05). Likewise, the total change in weight was similar in both DI (-4.35 kg) and DPA (-4.85 kg) groups (p > 0.05). A 5% reduction in initial body weight with DPA therapy resulted in a stronger correlation between the changes in irisin, visfatin, and FSTL1 levels and fasting glucose and HbA1c levels. These results demonstrate that serum irisin, FGF21, visfatin, and FSTL1 levels decreased in response to weight loss interventions. Weight loss induced by DI or DPA therapies had similar lowering effects on these proteins in subjects with IGM, and these myokines might be related to glucose metabolism biomarkers.

Dimensional change of impression materials for dental prothesis using different measuring methods

The aim of this study was to verify the effectiveness of alternative techniques for measuring the linear dimensional change of molds obtained by different impression materials. A stainless-steel die containing three lines was used to get the impressions. The impression materials were handled according to the manufacturers' instructions. After gelation/polymerization time, the molds (n=12) were stored for 30 minutes and the lines were subsequently measured using: a microscope (Olympus Measuring Microscope STM; Olympus Optical Co) with a 30x magnification (MM, control group), a digital caliper (Mitutoyo Digimatic; Kawasaki) (DC) and digitization using a digital camera (Canon EOS Rebel 3Ti; Canon) (DI). For each mold, a mean of the length of the lines was obtained. For each impression material, the difference in the length between the metallic matrix and the mold was considered as linear dimensional change (%). The data were submitted to the non-parametric Kolmogorov-Smirnov test, followed by one-way Analysis of Variance (ANOVA) for each material, and the means were compared by the Tukey test (α=0.05). The DI group promoted values of dimensional accuracy without significant differences with the control group (MM), except for the putty-body condensation silicone. The DC group showed significant differences for MM and DI groups for all impression materials tested. The DI group showed to be a reliable methodology (Standard error of the mean <10%) to measure the linear dimensional change of all impression materials. The DI group presented molds with linear dimensional change values without significant differences with MM group, except for the putty-body addition silicone. The digitization is a reliable methodology to evaluate the linear dimensional changes of molds made with impression materials, except for the putty-body elastomeric impression materials.

ВЛИЯНИЕ ИНТРАНАЗАЛЬНО ВВОДИМЫХ ИНСУЛИНА И С-ПЕПТИДА НА АКТИВНОСТЬ АМФ-АКТИВИРУЕМОЙ ПРОТЕИНКИНАЗЫ, МИТОХОНДРИАЛЬНУЮ ДИНАМИКУ И МАРКЕРЫ АПОПТОЗА В ГИПОТАЛАМУСЕ КРЫС СО СТРЕПТОЗОТОЦИНОВЫМ ДИАБЕТОМ

Most important factors leading to the development of diabetic encephalopathy in type 1 diabetes mellitus (T1DM) are impaired energy metabolism and mitochondrial dynamics, as well as activation of apoptosis in brain neurons, which is largely due to insulin and C-peptide deficiency in the CNS. In T1DM, hypothalamic neuronal dysfunctions lead to disturb both feeding behavior and central regulation of energy metabolism. One of the approaches to compensate for insulin and C-peptide deficiency in the brain is their intranasal administration, how- ever its influence on the functional state of hypothalamic neurons has not yet been studied. The aim of the work was to study the effect of intranasally administered insulin and C-peptide on the activity of AMP-activated protein kinase (AMPK) and expression of Drp-1 and mitofusins (Mfn-1 and -2) responsible for the biogenesis of mitochondria, pro- and anti-apoptotic proteins Bax and Bcl-2, autophagy-associated protein Beclin-1, and melanocortin receptors (MCR) in the hypothalamus of male rats with T1DM induced by 50 mg/kg of streptozotocin. We studied a 7-day intranasal administration to diabetic rats (D) of insulin (20 μ g/rat/day, DI), C-peptide (36 μ g/rat/day, DC), and insulin combined with C-peptide at two doses of 12 and 36 μ g/rat/day (DIC12, DIC36). In the hypothalamus of DI and DIC36 rat groups, there were observed normalization of AMPK activity and the Bax/Bcl-2 ratio (typically increased in T1DM), restoration of Drp-1, Mfn-2 and Beclin-1 expression, and enhancement of type 4 MCR expression responsible for transduction of anorexigenic signals, with all changes being associated with attenuated hyperphagia in these groups. In the DIС12 and DС rat groups, the restorative effects of the intranasal treatment were less pronounced. Thus, intranasal administration of insulin and C-pep- tide, to the greatest extent at a 1:3 molar ratio, restores energetic balance and mitochondrial dynamics, as well as suppresses pro-apoptotic processes in the hypothalamus of rats with severe T1DM. These effects appear to underlie their neuroprotective action.

Amino acid absorption and protein synthesis responses of turbot Scophthalmus maximus to lysine and leucine in free, dipeptide and tripeptide forms

The study aimed to investigate the effects of crystalline Lys and Leu, Lys‐Leu dipeptide and Lys‐Leu‐Lys/Leu‐Lys‐Leu tripeptide, on growth, postprandial Lys and Leu concentrations, gene expression of peptide and amino acid (AA) transporters and related gene expression of protein synthesis pathway in turbot. Three diets (CAA, Di and Tri) contained Lys and Leu as free (Lys and Leu), dipeptide (Lys‐Leu) and tripeptide (Lys‐Leu‐Lys/Leu‐Lys‐Leu) forms, respectively. Improved growth was observed in the Di group compared with the CAA group. For peptide and AAs transporters, PepT1, B⁰AT1 and y⁺LAT2 mRNA levels were affected in proximal intestine by dietary treatments. Free Lys and Leu concentrations in the CAA group were significantly higher than that of the Di and Tri groups at 6 hr postfeeding in serum and at 2, 6 and 24 hr postfeeding in muscle. For protein synthesis pathway in muscle, Akt2, TOR and S6k1 gene expression were the highest in the Di group and the lowest in the CAA group, whereas MuRF1 relative expression was the highest in the Tri group. In conclusion, dietary Lys‐Leu dipeptide was utilized more efficiently than free Lys and Leu or Lys‐Leu‐Lys/Leu‐Lys‐Leu tripeptide for turbot by regulating AAs transport, postprandial AAs concentration and the synthesis of muscle proteins.

Comparative evaluation of biochemical profiling of 4’, 4”- (4, 5, 6, 7-TETRAHYDRO- [1, 2, 3-] SELENADIAZOLO [4, 5e] PYRIDINE-4, 6-DIYL) BIS (BENZENE-1, 3-DIOL) and dipyrone on female rat’s

The current study has been carried out at the department of pharmaceutical chemistry, College of pharmacy. Novel synthetically selenium-containing compounds have potential therapeutic effects towards several diseases, such as: cancer microbial infections and neurodegenerative diseases. Therefore, the present study accentuated mainly on two significant items. A novel selenadiazole derivative i.e, 4’, 4”- (4, 5, 6, 7-TETRAHYDRO- [1, 2, 3-] SELENADIAZOLO [4, 5e] PYRIDINE-4, 6-DIYL) BIS (BENZENE-1, 3-DIOL) (T) and Dipyrone (Di) were used to detretmine their Biochemical effects on female rats. Biochemical test including; liver function tests; Renal functions tests; in addition lipid profile. Invivo study conducted using four groups, one as control (DW) and three treated groups (T, Di, and T&Di). The rats received 50mg/kg body weight (BW) of one of test treatments T and/or Di dissolved in 2 milliliter of distilled water and control group received same volume of distilled water for 30 days. Blood sample were collected directly from the rats heart under chloroform effect. The results indicated that Liver function test showed following results; Aspartate aminotransferase levels(AST) measurement it was cleared that only (T&Di) group (87.52 U/L ±12.20) was increased significantly than both DW(57.23 U/L ±10.43) and T(57.62 U/L ±16.54) groups. Alanine transaminase (ALT) concentration measurements showed only (T&Di) group (70.11 U/L ±13.09) value increased significantly than (DW), (T), (Di) groups. Alkaline Phosphatase (ALP) value of Di group (128.24 U/L ±27.9) highly elevated than in DW (66.68 U/L±15.29) and other test groups. Total protein (TP) concentrations of (Di) (4.97g/dL±1.02), (T) (10.87 g/dL ±3.25) and (T&Di) (5.05 g/dL ±0.76) groups highly reduced than (DW) group (14.80 g/dL ±1.98) level. Lipid profile test results show significant increase of Cholesterol (TC) level of (T) group (533.8mg/dL±52.5) than both DW (335.8mg/dL±27.01) and (T&Di) (390.3mg/dL±25.8) groups. Triglyceride (TG) serum levels only (T) group (100.1 mg/dL ±9.1) showed a significant reduction of TG value than in (Di) group (221.0572mg/dL ±39.8). Levels of HDL of (T) treated group (337.9 mg/dL±26.6) significant increased than all groups. VLDL levels results showed only Di group (43.4mg/dL±4.3) increased significantly than DW group; however there was significant decreased of T group VLDL level (20.03mg/dL±1.8) compare with Di (43.4mg/dL±4.3) and T&Di (35.9mg/dL±3.7) levels. Renal function data reveals significant reduction in blood urea levels of (T) (5.471 mg/dL ±3.745) and (T&Di) (10.633±5.431). Serum uric acid values showed significant decline of (T) group (2.601±0.743) than DW group (5.515±2.046). Also, the results of the present study illustrate only Di treated group (2.33±0.209) had essential increased of Creatinine values than all other study groups. The study concluded that synthesized novel selenadiazole derivative, and Dipyrone have mild effects on liver, kidney, and lipid profile. However, the companion of both drugs has some of undesirable effects.

Effects of different doses of dexmedetomidine administered intranasally on EC50 of propofol inhibiting responses to laryngeal mask airway insertion in pediatric patients

Objective To evaluate the effects of different doses of dexmedetomidine administered intranasally on the median effective target plasma concentration(EC50)of propofol inhibiting responses to laryngeal mask airway(LMA)insertion in the pediatric patients. Methods American Society of Anesthesiologists physical statusⅠor Ⅱ pediatric patients of both sexes, aged 1-3 yr, with body mass index of 20-26 kg/m2, scheduled for elective surgery under general anesthesia, were divided into 3 groups using a random number table: control group(group C), dexmedetomidine 1 μg/kg group(group D1)and dexmedetomidine 2 μg/kg group(group D2). At 20 min before induction of anesthesia, dexmedetomidine 1 and 2 μg/kg(diluted to 1 ml in normal saline)were intranasally administered in DI and D2 groups, respectively, and the equal volume of normal saline was intranasally administered in group C. Children were separated from their parents at 20 min after intranasal administration and admitted to the operating room.The target plasma concentration of propofol was determined by modified Dixon′s up-and-down method.The initial target plasma concentration of propofol was set at 5.4 μg/ml.The target plasma concentration of propofol was increased/decreased by 10% in the next patient according to the response to LMA insertion, and the ratio between the two successive concentrations was 1.1.Patients′ sedation status and LMA acceptance were evaluated when patients were separated from their parents.Patient′s satisfaction with sedation and with LMA acceptance was recorded.Probit analysis was used to calculate the EC50 of propofol inhibiting responses to LMA insertion. Results Compared with group C, the satisfactory rates of sedation and LMA acceptance were significantly increased, and EC50 of propofol inhibiting responses to LMA insertion was decreased in D1 and D2 groups(P<0.05). Compared with group D1, the satisfactory rates of sedation and LMA acceptance were significantly increased, and the EC50 of propofol inhibiting responses to LMA insertion was decreased in group D2(P<0.05). Conclusion Dexmedetomidine 1 and 2 μg/kg administered intranasally both can decrease the EC50 of propofol inhibiting responses to LMA insertion in the pediatric patients, and 2 μg/kg produces better efficacy. Key words: Dexmedetomidine; Administration, intranasal; Propofol; Dose-response relationship, drug; Laryngeal masks; Child

The pattern of archaeological cultures in northern China during the Eastern Zhou Period to the Qin Dynasty – also on the interactions among the Rong, Di and Hu ethnic groups and the Central Plains

Abstract Through the systematic trimming and analysis to the remains of the archaeological cultures of the Eastern Zhou Period through the Qin Dynasty in northern China, this paper puts forward that during this period, there were two cultural zones (the north and south cultural “belts”) with clearly different cultural features and connotations and peoples bearing clearly different physical characteristics in northern China, and discussed the regional differences of the remains of the archaeological cultures in each cultural belt and their developments and changes. The cultures in the south cultural belt could not be regarded as a part of the early Iron Age cultures in the Eurasian Steppes, but a kind of culture peculiar to the transitional zone between the cultures in the Eurasian Steppes and that in the Central Plains; the development and evolution of the north cultural belt, which emerged in the mid to the late Spring-and-Autumn Period, can be divided into three clear phases: the first phase was a part of the early Iron Age cultures in the Eurasian Steppes, but since the second phase, the cultural features and connotations of this belt began to stray out of the cultures in the Eurasian Steppes, which would be closely related to the military conquering and political management of the Central Plains polities and the powerful northward advance of the cultures of the Central Plains. Referring to the relevant historic literature, this paper made further observations to the interactions among the polities of the Central Plains and the peoples in these two cultural belts and the changes of the cultural patterns in each of the two cultural belts, and revealed the processes of the Sinicization of the Rong, Di and Hu ethnic groups in northern China. This paper pointed out that the Hu ethnic group lived in northern China since the mid Spring-and-Autumn Period, and the later appearance of the Hu people in the historic literatures was related to the northward advances of the territories of polities of the Central Plains rather than the southward invasion of the nomadic tribes living in the present-day Mongolian Plateau.