Stages Of Development Research Articles

Emerging Technologies and Applications of AI Chips: Integrating Deep Learning Algorithms with Advanced Hardware Architectures

Due to the ongoing promotion of the latest technological revolution and industrial upgrading, as well as the widespread use of artificial intelligence and deep learning, the intelligent Internet of Things terminal has reached a more advanced stage of development. The combination of training data, model algorithms, and processing power is the basis for advancing artificial intelligence. The semiconductor business plays a crucial role in shaping the future of AI algorithms and the computing power industry. This study explores the progression of artificial intelligence, and essential technologies, and examines the categorization and structure of AI chips. The process of deep learning was then analyzed, demonstrating the application of AI in real life. Next, this study showcases distinct AI chip-related models, specifically designed for the automated implementation of Convolutional Neural Networks (CNNs) on Field-Programmable Gate Arrays (FPGAs). The study then proceeds to examine the unique procedure and the significance of the resulting data. The future of AI was examined in the domains of space exploration, healthcare, and autonomous driving, along with future forecasts.

Global coupling of R-loopdynamics with RNA polymerase II modulates gene expression and early development of Drosophila.

R-loops are involved in many biological processes in cells, yet the regulatory principles for R-loops in vivo and their impact on development remain to be explored. Here, we modified the CUT&Tag strategy to profile R-loops in Drosophila at multiple developmental stages. While high GC content promotes R-loop formation in mammalian cells, it is not required in Drosophila. In contrast, RNAPII abundance appears to be a universal inducing factor for R-loop formation, including active promoters and enhancers, and H3K27me3 decorated repressive regions and intergenic repeat sequences. Importantly, such a regulatory relationship is dynamically maintained throughout development, and development-related transcription factors may regulate RNAPII activation and R-loop dynamics. By ablating Spt6, we further showed the global R-loop induction coupled with RNAPII pausing. Importantly, depending on the gene length, genes underwent up- or down-regulation, both of which were largely reversed by rnh1 overexpression, suggesting that R-loops play a significant role in the divergent regulation of transcription by Spt6 ablation. DNA damage, defects in survival, and cuticle development were similarly alleviated by rnh1 overexpression. Altogether, our findings indicate that dynamic R-loop regulation is dictated by RNAPII pausing and transcription activity, and plays a feedback role in gene regulation, genome stability maintenance, and Drosophila development.

Increasing the Oxygen Consumption in Hermetic Grain Storage Using Grain Weevils (Sitophilus granarius)

Loss of stored plant products due to insect infestation is a problem that is likely to increase with global warming. Improved storage under hermetic conditions in oxygen deficiency can prevent or control infestation and preserve product quality. Oxygen levels in hermetic storage decrease due to different factors, one of which is the oxygen consumption of the insects present. Experiments were carried out using varying numbers (25, 50, or 200) of all developmental stages of the grain weevil Sitophilus granarius L. (eggs, larvae, pupae, and adult beetles) caged and placed in sealed 30-L containers containing 22 kg of wheat for at least 21 weeks. Oxygen levels were measured at regular intervals. The oxygen consumption depended on the number of insects and went below the critical threshold of 3% for S. granarius survival in most of the trials. Some surviving beetles were observed at the end of the hermetic experiments and 12 weeks afterwards during control for progeny, when oxygen levels did not fall below the critical threshold or the low level could not be maintained for a sufficient time. Monitoring oxygen levels in hermetic storage is therefore essential to ensure safe storage over long periods.

Effect of Irrigation Scheduling on Growth, Yield and Irrigation Water use Efficiency of Linseed (Linum usitatissimum L.)

A field experiment was conducted to study the effect of irrigation scheduling on growth, yield and water use efficiency of linseed (Linum usitatissimum L.) at Main Agricultural Research Station (MARS), Raichur, during the rabi season of 2023-24. The experiment was laid out in a randomized complete block design with five treatments, replicated four times. There were five treatments viz., T1: Pre sowing irrigation only, T2: Pre sowing irrigation fb one irrigation at vegetative stage (30-35 DAS), T3: Pre sowing irrigation fb two irrigations at vegetative stage (30-35 DAS) and flowering stage (40-45 DAS), T4: Pre sowing irrigation fb three irrigations at vegetative stage (30-35 DAS), flowering stage (40-45 DAS) and capsule development stage (60-65 DAS) and T5: Rainfed condition. The results revealed that significantly higher plant height (60.94 cm), number of branches plant-1 (6.18), leaf area (36.94 cm2 plant-1), total dry matter production (16.41 g plant-1), number of capsules plant-1 (44.27), number of seeds (8.18 capsule-1), seed weight (2.98 g palnt-1), seed yield (1106 kg ha-1) and straw yield (2344 kg ha-1) was registered with pre-sowing irrigation fb three irrigations at vegetative stage (30-35 DAS), flowering stage (40-45 DAS) and capsule development stage (60-65 DAS) which was found to be on par with pre-sowing irrigation fb two irrigations at vegetative stage (30-35 DAS) and flowering stage (40-45 DAS). Further, (T4) recorded significantly lower water use efficiency (5.26 kg ha-mm-1). Whereas the treatment under rainfed conditions (T5) recorded significantly higher water use efficiency (13.59 kg ha-mm-1) than all other treatments.

Agricultural spray drone deposition, Part 1: Methods for high throughput spray pattern analysis

Abstract Effective pesticide application is dependent on precise and sufficient delivery of active ingredients to targeted pests. Water-sensitive papers (WSP) have been used to estimate the stain coverage, droplet density, droplet size, total spray volume, and other spray quality metrics by analyzing deposit stains using image analysis software. However, due to their expensive cost, WSPs are typically distributed along unidimensional transects at intervals of 0.5 m or more, which comprises 0.5% or less of the total treated area. This might limit the ability to accurately represent the deposition of agricultural sprayers with irregular patterns, such as agricultural drone sprayers in their early development stage. This study introduces a novel approach utilizing white Kraft paper and a blue colorant proxy for assessing spray deposition. A custom Python-based image analysis tool, SprayDAT (Spray Droplet Analysis Tool), was developed and compared to traditional image analysis software, DepositScan. Both models showed increased accuracy in detecting larger objects, with SprayDAT generally performing better for smaller droplets. DepositScan underestimated the total deposited spray volume by up to 2.7 times less compared to the colorant extraction assessed via spectrophotometry and the predicted output based on flow rate, coverage, and speed. Accuracy of software-estimated spray volume declined with increasing total stain coverage, likely due to overlapping stain objects. Droplet density exhibited a Gaussian trend with peak density at approximately 22% stain cover, offering evidence for overlapped stains for both DepositScan and SprayDAT as stain cover increased. Both models showed exponential growth in volumetric median diameter (VMD) with increasing stain cover. SprayDAT is freely accessible through an online repository. It features a user-friendly interface for batch-processing large sets of scanned images and offers versatility for customization to meet individual needs, such as adjusting spread factor, updating the standard curve for spray volume estimation, or modifying the stain detection threshold.

Design-Driven Sustainable Poverty Alleviation: A Case Study of Precise Identification and Resource Optimization in Rural Areas

China’s transition from precise poverty alleviation to rural revitalization represents a critical development stage. This study aims to explore how a design-driven approach can achieve sustainable poverty alleviation and development by addressing challenges such as relative poverty, imprecise targeting, insufficient endogenous motivation, and the risk of returning to poverty. Understanding the inherent relationship between poverty alleviation and sustainable development is crucial for deepening precise poverty alleviation strategies and promoting rural revitalization. This study employs the TOPSIS method to accurately identify target groups for poverty eradication and select optimal poverty alleviation strategies based on multidimensional poverty governance criteria. A case study of Village M in Guangdong Province is conducted, where questionnaire surveys and sampling methods are used to establish the TOPSIS model for precise identification and the design of targeted interventions to support poverty eradication. Both qualitative and quantitative approaches are applied to ensure comprehensive analysis and results. The findings indicate that targeted design interventions, particularly those leveraging local bamboo resources, led to a significant increase in the annual per capita income of identified families by approximately 176.32%, from RMB 3146 (approximately USD 431) to RMB 8700 (approximately USD 1192). This design-driven approach effectively stimulated the innovation consciousness of impoverished farmers, demonstrating the critical role of sustainable design strategies in optimally matching resources to the needs of the poor. This study contributes to the development of a novel framework integrating design methodologies with poverty alleviation strategies, providing empirical evidence on the effectiveness of these interventions. The findings highlight the importance of user-centered design in promoting sustainable development and underscore the need to incorporate design-driven methodologies in poverty alleviation efforts.

Transcriptomic analysis of the gonads of Locusta migratoria (Orthoptera: Acrididae) following infection with Paranosema locustae.

Paranosema locustae is an environmentally friendly parasitic predator with promising applications in locust control. In this study, transcriptome sequencing was conducted on gonadal tissues of Locusta migratoria males and females infected and uninfected with P. locustae at different developmental stages. A total of 18,635 differentially expressed genes (DEGs) were identified in female ovary tissue transcriptomes, with the highest number of DEGs observed at 1 day post-eclosion (7141). In male testis tissue transcriptomes, a total of 32,954 DEGs were identified, with the highest number observed at 9 days post-eclosion (11,245). Venn analysis revealed 25 common DEGs among female groups and 205 common DEGs among male groups. Gene ontology and Kyoto Encyclopaedia of Genes and Genome analyses indicated that DEGs were mainly enriched in basic metabolism such as amino acid metabolism, carbohydrate metabolism, lipid metabolism, and immune response processes. Protein-protein interaction analysis results indicated that L. migratoria regulates the expression of immune- and reproductive-related genes to meet the body's demands in different developmental stages after P. locustae infection. Immune- and reproductive-related genes in L. migratoria gonadal tissue were screened based on database annotation information and relevant literature. Genes such as Tsf, Hex1, Apolp-III, Serpin, Defense, Hsp70, Hsp90, JHBP, JHE, JHEH1, JHAMT, and VgR play important roles in the balance between immune response and reproduction in gonadal tissues. For transcriptome validation, Tsf, Hex1, and ApoLp-III were selected and verified by quantitative real-time polymerase chain reaction (qRT-PCR). Correlation analysis revealed that the qRT-PCR expression patterns were consistent with the RNA-Seq results. These findings contribute to further understanding the interaction mechanisms between locusts and P. locustae.

Possibilities of competitive displacement of Bactrocera dorsalis (Hendel) by Bactrocera zonata (Saunders) (Tephritidae: Diptera) in guava Psidium guajava ecosystem in Sudan

Oriental fruit fly (OFF) Bactrocera dorsalis and peach fruit fly (PFF) Bactrocera zonata are the most notorious pests that invaded Sudan in 2005 and 2012, respectively, causing severe losses and hindering horticultural production and exports. Prior to 2012, OFF was the most abundant fly in guava orchards in Gezira State. Monitoring fruit flies to determine species composition, seasonality, and possibilities of competitive displacement was conducted in two guava (Psidium guajava) orchards in Gezira State; Fadasi and Gezirat Elfil, using food bait attractant and rearing flies from infested fruits..Longevity and survival percentages of developmental stages of the two species were also determined. Monitoring fly populations using Torula yeast and rearing of fruit flies from guava fruits revealed that OFF and PFF were the most prevalent species in guava ecosystem at both sites. At the Fadasi site, PFF was the predominant species representing (99.3%) of the guild of the trapped flies with an infestation level of 99.6%) flies/kg of guava fruits. At G. Elfil, OFF comprised 80.8% of the trapped flies with infestation level ca 53.6% while 19.2% of trapped flies were B. zonata with 46.4% infestation level. The high percentage of trapped OFF at G. Elfil might be attributed to multi-cropping system in the area. Developmental stages of PFF were shorter than that of OFF by 9.2 days, while its survival percentage was higher than that of OFF for all developmental stages. Competitive displacement of OFF by PFF in guava orchards at Fadasi site may have occurred as a result of the mono-cropping system, short developmental period, and high survival percentage of developmental stages of PFF on guava compared to that of OFF.

Equipping prospective teachers for culturally and religiously sensitive sexuality education: curriculum reviews at Islamic universities in Indonesia

ABSTRACT Sexuality education is essential for equipping children to prevent sexual abuse and fostering understanding of their bodies, relationships, and boundaries. Early childhood education programs in Indonesia were recommended to integrate sexuality education courses tailored to child development and sensitive to religious and cultural contexts. This research employed qualitative methods to investigate the integration of sexuality education courses at eight Islamic universities in Indonesia. Data were collected through interviews with eight course instructors and documentation of their Semester Learning Plans, using content and thematic analysis. Findings revealed that while two universities offered dedicated sexuality education courses, six integrated them into child development and parenting psychology courses. Dedicated sexuality education courses had more comprehensive learning objectives compared to integrated approaches. Teaching methods predominantly included lectures, paper presentations, with some incorporating field studies and project-based learning. Challenges included credit allocation impacting study durations, which hindered the integration of specialized courses like sexuality education. Enhancing pedagogy and ensuring effective delivery of sexuality education were identified as crucial tasks requiring the refinement of curricula in early childhood education. This approach would enable prospective educators to impart knowledge sensitively, taking into account children’s developmental stages and cultural-religious norms.

Identification of genes associated with sex expression and sex determination in hemp (Cannabis sativa L.).

Dioecy in flowering plants has evolved independently many different times, and thus the genetic mechanisms underlying sex determination are diverse. In hemp (Cannabis sativa), sex is controlled by a pair of sex chromosomes (XX for females and XY for males). In an attempt to understand the molecular mechanism responsible for sex expression in hemp plants, we carried out RNA-Seq of male and female plants at different developmental stages. Using a pipeline involving differential gene expression analysis and weighted gene co-expression network analysis, we identified genes important for male and female flower development. We also demonstrate that sex-biased expression is already established at very early vegetative stages, before the onset of reproductive development, and several genes encoding transcription factors of the REM, bZIP and MADS family as candidate sex determination genes in hemp. Our findings demonstrate that the gene regulatory networks governing male and female development in hemp diverge already at a very early stage, leading to profound morphological differences in male and female hemp plants.

A comparative analysis of conventional echocardiographic findings among different heart failure stages and phenotypes: insights from the national echocardiographic society registry

Abstract Background The contemporary conceptualization of heart failure (HF) encompasses four developmental stages (at risk for HF, pre-HF, symptomatic HF, and advanced HF) and three distinct HF phenotypes categorized by left ventricular ejection fraction (LVEF) (HFpEF, HFmrEF, HFrEF). However, there is a need for a more comprehensive understanding of echocardiographic similarities and differences among pre-HF stages and various HF phenotypes. Material and Methods Our national echocardiographic society conducted a multicenter HF screening initiative. General practitioners in 13 primary care centers utilized the original mobile phone app to determine referrals for transthoracic echocardiography and N-terminal pro-B-type natriuretic peptide (NT-proBNP) testing in individuals without a prior HF diagnosis. The 2021 ESC guidelines' algorithms were used to diagnose HF phenotypes and 2016 EACVI/ASA recommendation² to assess diastolic function. This study defined "heart stress" (elevated NT-proBNP in asymptomatic individuals with risk factors, regardless of structural heart disease or cardiac dysfunction) and "patients at risk for HF" (normal NTproBNP and at least one HF risk factor). All patients were categorized into six groups: (I) no HF, no risk, (II) at risk for HF, (III) heart stress, (IV) HFpEF, (V) HFmrEF, and (VI) HFrEF. Echocardiographic findings were compared among groups (II) to (VI), using ANOVA, Kruskal-Wallis and Chi-squared test, when needed. Results In a cohort of 930 outpatients (mean age 66±11 years, 61% female), 34.2% were diagnosed with HF, 22.3% experienced heart stress, 37.8% were at risk for HF, and 12.8% had neither HF nor risk factors (Figure). While NT pro BNP showed progressive increase, LVEF showed a progressive decline across the groups, reaching its lowest in HFrEF patients (Table). Simultaneously, left ventricular mass index (LVMi), left atrial volume index (LAVi), and maximal velocity of tricuspid regurgitation (TR Vmax) significantly increased in a stepwise manner. Variations in predominant LV geometry were significant across groups (p<0.001): normal LV geometry in those without HF (60.8%), concentric LV remodeling in at-risk patients (38.6%), normal LV geometry in heart stress (49%), concentric LV hypertrophy (LVH) in HFpEF (27.7%), eccentric LVH in HFmrEF (42.3%), and both concentric and eccentric LVH in HFrEF (43.3% each). Diastolic dysfunction increased prevalence across the groups (12.9% vs 24.7% vs 37.6% vs 19.6% vs 51.1%, p<0.001). Remarkably, HFmrEF group had the highest proportion of undetermined diastolic function (14.7% vs 23.1% vs 27.9% vs 50% vs 26.7%, p<0.001). Conclusions The mobile phone app used in primary care setting allowed identification of significant number of individuals in the pre-HF stages, warranting further investigation. Distinct echocardiographic differences in pre-HF stages and HF phenotypes provide valuable insights for early detection and tailored treatments.

Exome sequencing identifies a novel FBN1 variant in a Chinese family with Marfan syndrome that includes aberrant right subclavian artery

Abstract Background Marfan syndrome (MFS) is an inherited autosomal dominant disorder that affects connective tissue with an incidence of about 1 in 5,000 to 10,000 people. Ninety percent of MFS is caused by mutations in the fibrillin-1 (fbn1) gene. We recruited a family with MFS phenotype in South China and identified a novel variant in fbn1 by whole exome sequencing (WES). The zebrafish share high genetic similarity to humans. This study aimed to generate this novel mutation in the fbn1 gene of zebrafish using the CRISPR/Cas9 technology and researched whether this variant is pathogenic. Methods A three-generation consanguineous family was recruited in this study, which was visited and interviewed for the clinical data and family history. Peripheral blood samples were collected from family members for DNA isolation and WES. The 3D structure of the protein was predicted by Alphafold. CRISPR/Cas9 was applied to generate an fbn1 nonsense mutation (fbn1+/−) in zebrafish. Morphological abnormalities were assessed in F2 fbn1+/− zebrafish by comparing with the wild-type (WT) controls at different development stages. Results Our study recruited a family with MFS phenotype in South China and identified a novel variant [NM_000138.5; c.7764C>G: p.(Y2588*)] in fbn1. Through Sanger sequencing, we found that family members Ⅲ-1 (son) and Ⅲ-2 (daughter) had the fbn1 variant segregated with MFS in the family. The p.Y2588* mutation resulted in the absence of 283 amino acids, thereby leading to the deficiency of 17 β-folded structures, 4 α-helix structures, and various loop structures. Compared with WT zebrafish, F2 fbn1+/− zebrafish exhibited aortic arches bleeding, abnormal angiogenesis, decreased cardiac volume, cardiac function defects, curly tail, and cartilage malformations. Conclusion A novel variant [NM_000138.5; c.7764C>G: p.(Y2588*)] was identified in fbn1 gene, which has not been reported in previous literature. The variant caused loss of function through premature protein truncation or nonsense-mediated mRNA decay. In zebrafish, we identified functional evidence of the detected nonsense mutation, which aligns with the clinical significance, suggesting a pathogenic variant of fbn1. Zebrafish as a novel, efficient tool to allow for the quick classification of unknown variants in fbn1 and improve the clinical diagnosis and treatment of MFS. It has brought the implementation of personal and precision medicine in the clinic within reach and made it possible to find patient-specific treatments.Marfanoid symptoms of the patient.Phenotypic Spectrum of fbn1+/− Zebrafish

Histological Analysis of Somatic Embryogenesis from Immature Zygotic Embryo of Wild Banana Musa acuminata ssp. malaccensis

Somatic embryogenesis, a crucial plant regeneration method, has become indispensable for crop improvement, particularly for species reliant on somatic cell manipulation techniques. Optimization of this process necessitates an understanding of the developmental stages involved. This study investigates the histological aspects of somatic embryogenesis in Musa acuminata ssp. malaccensis derived from immature zygotic embryos. Through detailed histological analysis, we aimed to elucidate the morphological changes and cellular organization occurring during the various stages of somatic embryogenesis, from induction, culture proliferation, and somatic embryo development to plantlet conversion. The initial stages of embryogenesis, characterized by nodules, were primarily composed of meristematic cells with high cell division activity. These cells contained tetrad-like structures that could develop into distinct two- and four-celled proembryoids or proembryogenic aggregates. Our histo-anatomical analysis revealed that embryogenic cultures proliferated through multiple pathways simultaneously: somatic embryo budding, proembryo formation, and pro-embryonic mass formation from both internal and peripheral cells. At the stage of somatic embryo development, embryos with a well-defined protoderm layer, containing cells with prominent nuclei and dense cytoplasm, potentially regenerate into plantlets. Furthermore, histological examination revealed the presence of procambium within mature somatic embryos, which subsequently developed into the vascular system of the complete plantlet

Developing a trustworthy national health data space: public needs and policy implications

Abstract Background The ongoing development of a national health data space in Switzerland presents an opportunity to transform health delivery and care by enabling large-scale secondary health research. Central to this development is the Health2030 strategy, which calls for the establishment of public trust in the reliability and security of data collection, storage, and exchange. Methods We conducted four focus groups across the German, French and Italian-speaking regions of Switzerland to inform the development of the Swiss health data space that fosters public trust. To inform Swiss policy, we held a panel discussion with patient experts and healthcare professionals to translate the focus group findings into governance and public communication requirements. We synthesized our findings using a thematic analysis approach and proposed a guiding health policy strategy. Results Citizens perceive the health data space as trustworthy conditional on the delivery of its promised higher levels of transparency, security, and lower risk compared to other data sharing initiatives. To ensure its trustworthiness, citizens call for the provision of value-based consent to control data access based on the actors involved and research purposes, alongside implementing accountability measures for data misuse. Governance framework requirements entail establishing an advisory group for the health data space with sufficient representation of the Swiss population, including members of the public, patient advisory groups, and researchers. Public communication requirements call for ongoing monitoring of public opinion on health data space, alongside establishing public engagement initiatives, such as public fora, to inform ongoing progress. Conclusions Policy efforts should prioritize the meaningful involvement of relevant stakeholders throughout all stages of the health data space development to foster trust. Central to these efforts is balancing public expectations with their practical implementation. Key messages • For the health data space to be trustworthy, citizens require transparency on their data use and the security measures taken, value-based consent for data access, and accountability measures. • Diverse representation in governance and continuous public involvement are key policy measures to build public trust in the development of national health data spaces.

Genome-wide identification and expression analysis of two-component system genes in switchgrass (Panicum virgatum L.)

The two-component system (TCS) consists of histidine kinase (HK), histidine phosphate transfer protein (HP), and response regulatory factor (RR). It is one of the most crucial components of signal transduction in plants, playing a significant role in regulating plant growth, development, and responses to various abiotic stresses. Although TCS genes have been extensively identified in a variety of plants, the genome-wide recognition and examination of TCS in switchgrass remain unreported. Accordingly, this study identified a total of 87 TCS members in the genome of switchgrass, comprising 20 HK(L)s, 10 HPs, and 57 RRs. Detailed analyses were also conducted on their gene structures, conserved domains, and phylogenetic relationships. Moreover, this study analysed the gene expression profiles across diverse organs and investigated their response patterns to adverse environmental stresses. Results revealed that 87 TCS genes were distributed across 18 chromosomes, with uneven distribution. Expansion of these genes in switchgrass was achieved through both fragment and tandem duplication. PvTCS members are relatively conservative in the evolutionary process, but the gene structure varies significantly. Various cis-acting elements, varying in types and amounts, are present in the promoter region of PvTCSs, all related to plant growth, development, and abiotic stress, due to the TCS gene structure. Protein-protein interaction and microRNA prediction suggest complex interactions and transcriptional regulation among TCS members. Additionally, most TCS members are expressed in roots and stems, with some genes showing organ-specific expression at different stages of leaf and inflorescence development. Under conditions of abiotic stress such as drought, low temperature, high temperature, and salt stress, as well as exogenous abscisic acid (ABA), the expression of most TCS genes is either stimulated or inhibited. Our systematic analysis could offer insight into the characterization of the TCS genes, and further the growth of functional studies in switchgrass.

Ex vivo magnetic resonance imaging of the human fetal brain.

The fetal brain undergoes a dynamic process of development during gestation, marked by well-orchestrated events such as neuronal proliferation, migration, axonal outgrowth, and dendritic arborization, mainly elucidated through histological studies. Ex vivo magnetic resonance imaging (MRI) has emerged as a useful tool for 3D visualization of the developing fetal brain, serving as a complementary tool to traditional histology. In this review, we summarized the commonly employed ex vivo MRI techniques and their advances in fetal brain imaging, as well as a standard protocol for postmortem fetal brain specimen collection and fixation. We then provided an overview of ex vivo MRI-based studies on the fetal brain. According to our review, ex vivo T1- or T2-weighted structural MRI has contributed to the characterization of the anatomy of transient neuronal proliferative zones, the basal ganglia, and the cortex. Diffusion MRI related techniques, such as diffusion tensor imaging and tractography, have helped to investigate the microstructural patterns of fetal brain tissue, as well as the early emergence and development of neuronal migration pathways and white matter bundles. Ex vivo MRI findings have shown strong histological correlations, supporting the potential of MRI in evaluating the developmental events in the fetal brain. Postmortem MRI examinations have also demonstrated comparable, and in certain cases, superior performance to traditional autopsy in revealing fetal brain abnormalities. In conclusion, ex vivo fetal brain MRI is an invaluable tool that provides unique insights into the early stages of brain development.

More than flowering: CONSTANS plays multifaceted roles in plant development and stress responses.

Plants have evolved a remarkable ability to sense and respond to changes in photoperiod, allowing adjustments to their growth and development based on seasonal and environmental cues. The floral transition is a pivotal stage in plant growth and development, signifying a shift from vegetative to reproductive growth. CONSTANS (CO), a central photoperiodic response factor conserved in various plants, mediates day-length signals to control the floral transition, although its mechanisms of action vary among plants with different day-length requirements. In addition, recent studies have uncovered roles for CO in organ development and stress responses. These pleiotropic roles in model plants and crops make CO a potentially fruitful target for molecular breeding aimed at modifying crop agronomic traits. This review systematically traces research on CO, from its discovery and functional studies to the exploration of its regulatory mechanisms and newly discovered functions, providing important insight into the roles of CO and laying a foundation for future research.

8.A. Scientific session: Engage, co-create, collaborate, transform: uniting practice and research for health innovation

Abstract Background Public health practitioners are increasingly challenged by theoretical, political, and contextual changes and innovations. To ensure that innovations are sustainable, it is important not only to include stakeholders (e.g. end-users, implementers, policy-makers), but also to actively involve them in the development, implementation and transformation of public health innovations. Participatory action research, co-creation and stakeholder engagement are proven methods to co-produce effective innovations, strengthen existing networks and promote implementation by increasing fit and applicability in practice. In this workshop, our overall aim is to bring together practitioners and researchers to improve mutual understanding and adapt practices in development, implementation and transformation of innovations. The objectives of the workshop are: 1) to increase participants’ understanding of the circumstances under which stakeholder involvement is most meaningful, and 2) to increase participants’ awareness of tools, strategies or theories to overcome challenges in stakeholder involvement. Workshop format The workshop consists of two parts: four interactive presentations that introduce important development steps of public health innovation and a problem-based discussion using a real-life case that builds upon the presentations. Interactive presentations describe (1) Theory of Change (ToC) approach for active collaboration with stakeholders, (2) co-creation of a serious game for and with healthcare providers, (3) visualising collaboration through Social Network Analysis to improve implementation, and (4) enhancing scale up with PRACTIS and Design Thinking. During the presentations, interactive tools are used to help identify the characteristics of the workshop participants (e.g. work setting, type of stakeholders), as well as their interests and needs (e.g. challenges faced, methods used). During the second part of the workshop, a health innovation is chosen from a number of pre-selected innovations from European contexts (e.g. BOB campaign, Schools4Health, LETHE) that aligns most with participants’ background and interests. Next, the main goal and known challenges within these innovations are introduced, which aids participants to formulate intermediate goals using the ToC approach. Then, they answer questions and make decisions for the discussed stages of innovation development (e.g. “Which gaps exist in the current network and how can we address them to improve collaboration?”, ‘What scalability challenges exist in this innovation and how can PRACTIS help identify them?”). In answering these questions, participants will share their knowledge and expertise with each other, while the chair facilitates the discussion. We are flexible in the format of the challenge-based discussion; depending on the number of attendees, we may work with a plenary discussion or smaller groups that work independently under the supervision of 1 or 2 facilitators each. Key messages • Non-linear approaches are needed to take into account the complicated and changing systems in which health innovation takes place. • Theory of change, co-creation, social network analysis, PRACTIS and design thinking offer practical guidance for stakeholder involvement in specific phases of health innovations.

Open Innovation and Regulatory Challenges in New Modality Development: The Pivotal Role of Contract Development and Manufacturing Organisations in Advancing Antibody Drugs.

Ensuring regulatory-compliant manufacturing capability is an essential challenge for new treatment modalities, but its internalisation is not easy for pharmaceutical companies, especially start-ups. This study examines the functions and requirements of contracted development and manufacturing organisations (CDMOs) using the development process of antibody medicines as a case study. Utilizing PubMed, Cortellis and Patent Integration databases, this study delves into publication and contractual trends in monoclonal antibody drugs (mAbs) development, alongside an analysis of patent filings by CDMOs, offering a comprehensive overview of the evolving landscape in mAbs innovation. In the early stages of mAbs development, dedicated bio firms (DBFs) led R&D with superior drug discovery technology but lacked manufacturing capability, which was complemented by CDMOs. This collaboration was an opportunity for CDMOs to expand their capabilities beyond manufacturing technology into antibody drug candidate discovery and structural optimisation technology. From mid-development onwards, it established a technology platform based on these capabilities and developed and established partnerships with existing pharmaceutical companies, including mega pharma. The impact of institutions and regulations on the innovation process was assessed during this development process. These findings are expected to provide valuable insights into the innovation system for new modalities.

Reconsidering Fromm and feminisms, part II: a case for psychosocial compatibilities

This article continues an earlier one about how the work of Erich Fromm and feminist theories are compatible despite the connection usually remaining overlooked. Drawing on Fromm’s critique of Freud’s analysis of Dora, but also going further, I argue that associating Fromm and contemporary feminist thought is particularly important in the context of growing authoritarianism on the right and sometimes as reflected also on the left. The article elaborates upon five points of ‘psychosocial compatibility’ between Fromm’s humanistic vision and the ideas of several well-known feminist theorists including Simone de Beauvoir, Nancy Chodorow, Jessica Benjamin and others. The five points of psychosocial compatibility addressed between From and contemporary feminisms as addressed here are: connecting the personal and the political while stressing the prefigurative; kindred issues concerning objectification and self-love; emphases on relatedness and the pre-oedipal stage of human development; concerns about multi-dimensionality and intersectionality; and shared political beliefs aimed at overcoming gender and other oppressive inequalities