Developmental Processes Research Articles

Expected Eight-Week Prenatal Versus Twelve-Week Perinatal Tenofovir Alafenamide Prophylaxis to Prevent Mother-To-Child Transmission of Hepatitis B Virus: A Multicenter, Prospective, Open-label, Randomized Controlled Trial.

The course of maternal antiviral prophylaxis to prevent mother-to-child transmission of hepatitis B virus (HBV-MTCT) varies greatly, and it has not been demonstrated in a randomized controlled study. In this multicenter, open-label, randomized controlled trial, eligible pregnant women with HBV DNA of 5.3-9.0 log10 IU/mL who received tenofovir alafenamide fumarate (TAF) from the first day of 33 gestational weeks to delivery (expected eight-week) or to four-week postpartum (expected twelve-week) were randomly enrolled at a 1:1 ratio and followed until six-month postpartum. All infants received standard immunoprophylaxis (hepatitis B immunoglobulin and vaccine). The primary endpoint was the safety of mothers and infants. The secondary endpoint was infants' HBV-MTCT rate at seven months of age. Among 119 and 120 intention-to-treat pregnant women, 115 and 116 women were followed until delivery, and 110 and 112 per-protocol mother-infant dyads in two groups completed the study. Overall, TAF was well tolerated, no one discontinued therapy due to adverse events (0/239, 0%, 95% confidence interval [CI] 0%-1.6%), and no infant had congenital defects or malformations at delivery (0/231, 0%, 95% CI 0%-1.6%). The infants' physical development at birth (n=231) and at seven months (n=222) were normal. Furthermore, 97.0% (224/231, 95% CI 93.9%-98.5%) of women achieved HBV DNA <5.3 log10 IU/mL at delivery. The intention-to-treat and per-protocol infants' HBV-MTCT rates were 7.1% (17/239, 95% CI 4.5%-11.1%) and 0% (0/222, 95% CI 0%-1.7%) at seven months of age. Comparatively, 15.1% (18/119, 95% CI 9.8%-22.7%) versus 18.3% (22/120, 95% CI 12.4%-26.2%) of women in the two groups had mildly elevated alanine aminotransferase levels at three-month and six-month postpartum, respectively (P=0.507); notably, no one experienced alanine aminotransferase flare (0% [0/119, 95% CI 0%-3.1%] versus 0% [0/120, 0%-3.1%]). Maternal TAF prophylaxis to prevent HBV-MTCT is generally safe and effective, and expected eight-week prenatal duration is feasible. ClinicalTrials.gov, NCT04850950.

Both Ends of the Leash: Animals in Australian Residential Aged Care Facilities, Views of Experienced Animal Trainers/handlers Working in Residential Aged Care Facilities.

Research on animals in residential aged care facilities (RACFs) mainly focuses on the residents with little concern for animal welfare. Australian RACFs cannot care for residents, making it unlikely they can maintain animal welfare. The One-Health/One-Welfare frameworks support developing animal policies in RACFs. However, inadequate policies places residents and animals at risk. To understand animals' lived experiences in RACFs, experienced dog-trainers who worked in RACFs were interviewed. These experts were recruited using snowball sampling, with nine participating in semi-structured interviews providing opinions and experiences of visiting and live-in animals in Australian RACFs. The initial inductive coding produced six themes (residents, animals, handlers, staff, facility and policy). However, they noted animal welfare issues and explored them further using deductive coding, re-coding the quotes into four areas: handler, environment, participants and intervention. When residents were the focus of animal interactions, animal welfare became compromised, with dogs being frightened and one accidentally poisoned. Animals in RACFs could suffer poor welfare because of inadequate staffing, training and oversight. To provide safe, ethical animal contact, legislation and guidelines must support policy development for animals in RACFs.

Developmental change, bricolage, and how a lot of things develop: Mechanisms and changes in attachment across the lifespan.

Under the leadership of its founding editor, Dante Cicchetti, Development and Psychopathology has been recognized for decades as the foremost journal integrating developmental theory and clinical research programs. Contributors have often highlighted the implications of attachment theory and research for understanding developmental processes and pathways, and as a testing ground for intervention strategies. In this paper we reflect on the strengths and limitations of the traditional developmental perspective. We suggest that behavioral, cognitive, and emotional development are better understood as a process of bricolage (construction within constraints). This perspective is illustrated in an analysis of change mechanisms, and behavioral and representational changes, in attachment development from pre-locomotor infancy to later adulthood. Special emphasis is placed on ordinary learning and cognitive processes, rather than those specific to attachment, and on the roles that socialization pressures and changing circumstances play in shaping the course of attachment development.

An interview with Karen Sears.

Karen Sears is a Professor of the Department of Ecology and Evolutionary Biology and the Department of Molecular, Cellular, and Developmental Biology at the University of California, Los Angeles (UCLA), USA. Her group leverages a range of organisms, including bats and the short-tailed opossum, to study the mechanisms underpinning mammalian evolution. Karen is a Guest Editor for Development's Special Issue on Uncovering Developmental Diversity, which highlights how unconventional model organisms are advancing our understanding of developmental biology. We caught up with Karen to find out more about her research, the importance of public engagement and the challenges of working with unusual organisms in the lab.

Interpreting, translating, and embedding school curriculum in physical education teacher education: the need for collective action within and across the teacher education continuum

ABSTRACT Olson, J. K. [1977. “Teacher Education and Curriculum Change: Reexamining the Relationship.” Curriculum Inquiry 7 (1): 61–66]. https://doi.org/10.1080/03626784.1977.11076205. argued how in the wave of curriculum development, the role of teachers, and therefore initial teacher education (ITE), had been neglected and as such, they provided a vision for a revised form of ITE. We contend that almost 50 years on, the ITE community is yet to fulfil this vision for ITE. We explore this argument through the recent wave of physical education curriculum policy development, particularly the introduction of Leaving Certificate Physical Education (LCPE), and the processes of interpretation, translation, and embedding curriculum into ITE. We were guided by the research question: How are physical education teacher educators embedding curriculum into their Physical Education Teacher Education (PETE) programmes to best prepare pre-service teachers (PSTs) to teach such curriculum? Seventeen physical education teacher educators across three ITE institutes were interviewed on the processes of embedding LCPE into their respective programmes. Findings discuss the necessity for PETE programme reconfiguration, the (mis)alignment between PETE and current realities of PSTs, schools, and society, and highlight the need for advocacy and sharing across PETE. This paper advocates for teacher educators to be positioned as policy actors, pedagogical possibilities in PETE, and the need for different stakeholders to work (and learn) together.

Basketball-Based Physical Education on Students’ Physical Fitness in a Selected Technical Vocational College, Fujian, China

The role of basketball-based physical education in enhancing students' physical fitness has been extensively documented, with numerous studies highlighting its benefits. In China, where sports and physical education are integral components of the educational curriculum, the importance of understanding the specific impacts of basketball-based physical education cannot be overstated. This study focuses on the technical vocational college setting in Fujian, China, aiming to fill a crucial research gap and address the need for tailored physical education programs. Basketball has been widely promoted and accepted in major universities across China, garnering broad support from both students and faculty. Wang (2024) emphasizes that with its increasing incorporation into university physical education programs, basketball significantly impacts students' physical fitness and athletic abilities. The current educational reforms in China necessitate the continual optimization of teaching methods and approaches to establish a solid foundation for the physical and mental development of students. Sui (2024) explores the impact of community basketball programs on high school students' physical and mental health in Guangzhou, highlighting improvements in physical fitness, social skills, and mental well-being. This study underscores the broader benefits of basketball beyond mere physical health, emphasizing its role in fostering social interaction and emotional resilience. Additionally, Shao et al. (2022) examine the individual characteristics of physical and mental development and their connection with regular physical exercises when playing basketball. Their findings suggest that basketball enhances motor fitness, moral, volitional, and mental qualities, as well as creativity and decision-making skills in rapidly changing circumstances. This holistic development is crucial for students, particularly in a competitive and fast-paced society like China. Butenko (2023) presents the effectiveness of a comprehensive use of sports games, including basketball, during extracurricular activities for high school students. The study found significant improvements in physical performance, physical qualities, and health indicators, demonstrating the high potential of integrating sports games into physical education programs to enhance students' overall fitness and health. As a teacher and coach in a technical vocational college in Fujian, China, the researcher has observed the transformative impact of sports, particularly basketball, on students' lives. Professional experience has shown that students engaged in basketball not only develop better physical fitness but also exhibit improved teamwork, leadership skills, and emotional resilience. These observations have fueled the researcher's passion for integrating basketball into the physical education curriculum and inspired a systematic exploration of its broader impacts. The researcher's personal motivation stems from a deep-seated belief in the power of sports to shape character and foster holistic development. Having been a basketball player, the researcher has experienced the discipline, dedication, and camaraderie that the sport instills. These values are essential for students, especially those in technical vocational education, as they prepare for their future careers and personal lives. Despite the known benefits of basketball-based physical education, there is a significant gap in research specifically targeting technical vocational colleges in China. Most existing studies focus on general high school or university settings, leaving a void in understanding how basketball impacts students in technical vocational education, who may have different needs and challenges. This study addresses this gap by focusing on a technical vocational college in Fujian, China. It aims to provide evidence-based insights into how basketball-based physical education influences various aspects of students' physical fitness, including physical attributes development, motor skills enhancement, cardiovascular fitness, strength and power development, flexibility and agility, coordination and balance, mental and emotional benefits, social interaction and teamwork, skill development and performance, and health and fitness awareness. By filling this research gap, the study seeks to inform policy and practice in physical education, providing valuable recommendations for optimizing physical education programs to enhance the health and fitness outcomes of vocational students.

Role of Gonadal Steroid Hormones in the Eye: Therapeutic Implications

Gonadal steroid hormones are critical regulatory substances involved in various developmental and physiological processes from fetal development through adulthood. These hormones, derived from cholesterol, are synthesized primarily by the gonads, adrenal cortex, and placenta. The synthesis of these hormones involves a series of enzymatic steps starting in the mitochondria and includes enzymes such as cytochrome P450 and aromatase. Beyond their genomic actions, which involve altering gene transcription over hours, gonadal steroids also exhibit rapid, nongenomic effects through receptors located on the cell membrane. Additionally, recent research has highlighted the role of these hormones in the central nervous system (CNS). However, the interactions between gonadal steroid hormones and the retina have received limited attention, though it has been suggested that they may play a protective role in retinal diseases. This review explores the synthesis of gonadal hormones, their mechanisms of action, and their potential implications in various retinal and optic nerve diseases, such as glaucoma, age-related macular degeneration (AMD), diabetic retinopathy (DR), or retinitis pigmentosa (RP), discussing both protective and risk factors associated with hormone levels and their therapeutic potential.

Field-based physical fitness assessment in preschool children inChina.

Physical fitness in the preschool years is considered a marker of current and future health. Therefore, understanding physical fitness levels of preschool children is necessary. Furthermore, the use of field-based methods to measure physical fitness is essential for assessing children in authentic venues such as schools. Hence, the main purpose of this study was to describe the physical fitness of Chinese preschool children across field-based methods considering a statement of confidence intervals for reference in physical fitness in each age and genders. A total of 619 children (296 boys and 323 girls) aged 3-6years old from China participated in the present study. Physical fitness was measured using the Chinese National Physical Fitness Measurement (CNPFM-Pre) battery, which included the tennis ball throw (upper-limb muscular strength), standing long jump (lower-limb muscular strength), shuttle run test (speed-agility), double-leg timed hop (coordination), sit-and-reach (flexibility), and balance beam test (dynamic balance). The results revealed significant differences in gender for standing long jump (SLJ; η2 = 0.012, p = 0.007), tennis ball throw (TT; η2 = 0.081, p < 0.001), sit-and-reach (SR; η2 = 0.114, p < 0.001), and one-legged stance test (OST; η2 = 0.037, p < 0.001). Age had positive effects on all physical fitness performance tests, as the shuttle run test (η2 = 0.672, p < 0.001), SLJ (η2 = 0.513, p < 0.001), TT (η2 = 0.394, p < 0.001), double-legged timed hop test (DTH; η2 = 0.445, p < 0.001), SR (η2 = 0.069, p < 0.001), balance beam test (BBT; η2 = 0.367, p < 0.001), and OST (η2 = 0.336, p < 0.001). Older preschool children performed better than their younger counterparts in all the tests. No significant interactions between age groups and gender were found. This study provided age- and gender-specific physical fitness performance data in Chinese preschool children. Gender differences in physical fitness tests performance already exists at the preschool age. All physical fitness component results increased with age in this cohort. These findings can help healthcare, physical education, and pediatric professionals understand the physical fitness development of preschool children at different ages and help design sports tasks for preschool boys and girls.

THE DEVELOPMENT OF STRENGTH QUALITIES OF YOUNG PEOPLE IN PHYSICAL EDUCATION LESSONS

The article raises the issues of the development of strength abilities as a directed pedagogical process. The author conducted a study on the development of strength qualities of boys and girls aged 16-17 who are engaged in physical education classes, and carried out a practical experiment on the development of strength abilities among 16-17-year-old people. As a result of this experiment, the author came to a conclusion that the use of exercises for the development of strength and endurance makes it possible to lay the foundations for further physical development of young people. At this stage, the skills of precise exercise performance are formed, the principles of proper coordination of movements are applied. The training programme includes exercises that require cyclic movements.

Identifying the role of siblings in adolescent decision-making: A developmental processes approach.

Adolescence represents a period of opportunity in decision-making as developmental motivations and processes shift, particularly in social contexts. Sibling relationships may be especially influential in adolescent decision-making, as siblings spend a disproportionate amount of time with one another and can affect how adolescents think, feel, and behave. However, the ways in which siblings may shape adolescent decision-making through psychological, behavioral, and neurological processes, particularly their interactions, are less well known. This review introduces the developmental processes approach to understanding adolescent decision-making in the sibling context by reviewing patterns in the literature as to how each developmental process in sibling contexts may contribute to adolescent decision-making. Interdisciplinary collaborations across theories and methods (i.e., surveys, observations, and neuroimaging) can contribute to a more holistic understanding of how siblings may shape adolescent decision-making, for better and for worse. Further, this approach can inform practice through sibling-focused prevention programs and other sibling-focused programming (e.g., government, nonprofit) in promoting adaptive adolescent decision-making.

Guiding Students to Think Critically about Reproductive Development and Public Conversations on Sex and Gender.

Developmental Biology is intricately connected to current issues of societal and political importance. Bringing these connections into the classroom makes the biology topics relevant to our students and, when students find classroom topics relevant, they are more likely to think critically about the material. In addition, this type of engagement encourages students to remain in science majors and pursue careers in biological and biomedical fields. The use of guided inquiry methodology, scaffolded discussions, and a variety of source material on sex and gender, has been successful in engaging students in an undergraduate Developmental Biology course.

The Composition and Function of Intestinal Microbiota Were Altered in Farmed Bullfrog Tadpoles (Aquarana catesbeiana) during Metamorphosis

The bullfrog Aquarana catesbeiana is one of the main farmed frog species in China, with a low overall survival of farmed bullfrogs from hatching to harvest since bullfrog tadpoles are fragile during the metamorphosis period. The intestinal bacterial community can play crucial roles in animal development; however, little is known about the alteration of the gut microbial community of A. catesbeiana during metamorphosis. The present study used 16S rRNA amplicon sequencing to investigate the intestinal bacterial community in A. catesbeiana at four distinct developmental stages. Moreover, we determined the bullfrog’s body morphological parameters and the intestine histology at different developmental stages. The results showed a reduction in the total length and snout–vent length of A. catesbeiana during metamorphosis. The intestinal microbial composition of A. catesbeiana exhibited variation throughout the process of metamorphosis. The terrestrial stage showed shifts in the bacterial composition compared to the aquatic stages, including a reduction in Bacteroidetes and an increase in Firmicutes. Furthermore, the presence of Prevotella, Bifidobacterium, Leucobacter, Corynebacterium, Bulleidia, Dorea, Robinsoniella, and Clostridium in A. catesbeiana metamorphosis appears to be mainly related to the host’s epithelial cells’ height and total body mass. The results indicated that the intestinal microbial composition changed with the bullfrog–tadpole metamorphosis. The genera of Prevotella, Bifidobacterium, Leucobacter, Corynebacterium, Bulleidia, Dorea, Robinsoniella, and Clostridium might be potential probiotics.

Polyamines and hydrogen peroxide: Allies in plant resilience against abiotic stress.

The increasing prevalence and severity of abiotic stresses on plants due to climate change is among the crucial issues of decreased crop productivity worldwide. These stresses affect crop productivity and pose a challenge to food security. Polyamines (Pas) and hydrogen peroxide (H₂O₂) could play a vital role to minimize the impact of several abiotic stresses on the plants. Pas are small molecules that regulate various physiological and developmental processes in plants and confer stress tolerance and protection against dehydration and cellular damage. Pas also interact with plant growth regulators and participate in various signaling routes that can mediate stress response. H₂O₂ on the other hand, acts as a signaling agent and plays a pivotal part in controlling crop growth and productivity. It can trigger oxidative damage at high levels but acts as a stress transducer and regulator at low concentrations. H₂O₂ is involved in stress defense mechanisms and the activation of genes involved in conferring tolerance. Therefore, the main focus of this paper is to explore roles of Pas and H₂O₂ in plant responses to various abiotic stress, highlighting their involvement in stress retaliation and signaling routes. Emphasis has been placed on understanding how Pas and H₂O₂ function and interact with other signaling molecules. Also, interaction of Pas and H₂O₂ with calcium ions, abscisic acid and nitrogen has been discussed, along with activation of MAPK cascade. This additive understanding could contribute to adopt strategies to improve crop productivity and enhance plant resilience to environmental challenges.

Female-age-dependent changes in the lipid fingerprint of the mammalian oocytes.

Can oocyte functionality be assessed by observing changes in their intracytoplasmic lipid droplets (LDs) profiles? Lipid profile changes can reliably be detected in human oocytes; lipid changes are linked with maternal age and impaired developmental competence in a mouse model. In all cellular components, lipid damage is the earliest manifestation of oxidative stress (OS), which leads to a cascade of negative consequences for organelles and DNA. Lipid damage is marked by the accumulation of LDs. We hypothesized that impaired oocyte functionality resulting from aging and associated OS could be assessed by changes in LDs profile, hereafter called lipid fingerprint (LF). To investigate if it is possible to detect differences in oocyte LF, we subjected human GV-stage oocytes to spectroscopic examinations. For this, a total of 48 oocytes derived from 26 young healthy women (under 33 years of age) with no history of infertility, enrolled in an oocyte donation program, were analyzed. Furthermore, 30 GV human oocytes from 12 women were analyzed by transmission electron microscopy (TEM). To evaluate the effect of oocytes' lipid profile changes on embryo development, a total of 52 C57BL/6 wild-type mice and 125 Gnpat+/- mice were also used. Human oocytes were assessed by label-free cell imaging via coherent anti-Stokes Raman spectroscopy (CARS). Further confirmation of LF changes was conducted using spontaneous Raman followed by Fourier transform infrared (FTIR) spectroscopies and TEM. Additionally, to evaluate whether LF changes are associated with developmental competence, mouse oocytes and blastocysts were evaluated using TEM and the lipid dyes BODIPY and Nile Red. Mouse embryonic exosomes were evaluated using flow cytometry, FTIR and FT-Raman spectroscopies. Here we demonstrated progressive changes in the LF of oocytes associated with the woman's age consisting of increased LDs size, area, and number. LF variations in oocytes were detectable also within individual donors. This finding makes LF assessment a promising tool to grade oocytes of the same patient, based on their quality. We next demonstrated age-associated changes in oocytes reflected by lipid peroxidation and composition changes; the accumulation of carotenoids; and alterations of structural properties of lipid bilayers. Finally, using a mouse model, we showed that LF changes in oocytes are negatively associated with the secretion of embryonic exosomes prior to implantation. Deficient exosome secretion disrupts communication between the embryo and the uterus and thus may explain recurrent implantation failures in advanced-age patients. Due to differences in lipid content between different species' oocytes, the developmental impact of lipid oxidation and consequent LF changes may differ across mammalian oocytes. Our findings open the possibility to develop an innovative tool for oocyte assessment and highlight likely functional connections between oocyte LDs and embryonic exosome secretion. By recognizing the role of oocyte LF in shaping the embryo's ability to implant, our original work points to future directions of research relevant to developmental biology and reproductive medicine. This research was funded by National Science Centre of Poland, Grants: 2021/41/B/NZ3/03507 and 2019/35/B/NZ4/03547 (to G.E.P.); 2022/44/C/NZ4/00076 (to M.F.H.) and 2019/35/N/NZ3/03213 (to Ł.G.). M.F.H. is a National Agency for Academic Exchange (NAWA) fellow (GA ULM/2019/1/00097/U/00001). K.F. is a Diamond Grant fellow (Ministry of Education and Science GA 0175/DIA/2019/28). The open-access publication of this article was funded by the Priority Research Area BioS under the program "Excellence Initiative - Research University" at the Jagiellonian University in Krakow. The authors declare no competing interest. N/A.

Community-engaged research: Bringing the science of developmental psychopathology into the real world.

The science of developmental psychopathology has made outstanding progress over the past 40 years in understanding adaptive and maladaptive developmental processes across the life span. Yet most of this work has been researcher driven with little involvement of community partners in the research process, limiting the potential public health significance of our work. To continue to advance the field we must move beyond the physical and conceptual walls of our research laboratories and into the real world. In this article, we define and describe the importance of community-engaged research, and present our overarching principles for engaging the community including practicing respect, shared power and decision-making, prioritizing the needs of the community, and engaging in consistent and transparent communication. We present several associated recommendations for best practice and highlight examples from our own research that is grounded in a developmental psychopathology perspective to illustrate these practices. Recommendations for the future of the discipline of development and psychopathology, with emphasis on training and continuing education, are described.

Fat deposition and body mass components of Mongolian urban children and adolescents living in various social conditions

Introduction. The purpose of the study is to characterize and to compare the indicators of fat deposition and body composition in groups of urban children and adolescents of the Republic of Mongolia in various living conditions. Materials and methods. The material for this study was the results of a comprehensive survey of 5 612 Mongolian children and adolescents aged 9-17 years (born and permanently residing in the capital city of Ulaanbaatar). In accordance with the objectives of the study, the body circumferences, the skinfolds, as well as the body mass components evaluated by bioimpedance technique were analyzed. Results. Based on the obtained statistical parameters of the studied features, as well as on the results of their comparison in the studied groups, it can be concluded that the value of most of the considered indicators in children and adolescents living in apartments is higher than in their peers living in yurts. Discussion. The increase in body weight in boys living in the apartments, recorded according to the results of our previous work, is associated with an increase in active cell mass, and, as a result, – lean body mass. To a lesser extent, judging by the existing differences, its value is influenced by the fat component. While the parameters of fat deposition show a small number of significant differences, unlike circumferences, it can also be concluded that the variability of the girths is more associated with the development of the muscular component. Similar patterns were obtained for girls. However, in this case they are less pronounced, but also correspond to the previously obtained results. Conclusion. The general increase in the welfare of the population of Ulaanbaatar and significant improvement in the living conditions of the younger generation, has a positive effect on the youth physical development, primarily due to a more pronounced increase in the metabolically active components of body weight. This may be considered as an important reflection of the socio-political processes taking place in the country. © 2024. This work is licensed under a CC BY 4.0 license

Molecular composition of skeletal muscle in infants and adults: a comparative proteomic and transcriptomic study

To gain a deeper understanding of skeletal muscle function in younger age and aging in elderly, identification of molecular signatures regulating these functions under physiological conditions is needed. Although molecular studies of healthy muscle have been conducted on adults and older subjects, there is a lack of research on infant muscle in terms of combined morphological, transcriptomic and proteomic profiles. To address this gap of knowledge, we performed RNA sequencing (RNA-seq), tandem mass spectrometry (LC–MS/MS), morphometric analysis and assays for mitochondrial maintenance in skeletal muscle biopsies from both, infants aged 4–28 months and adults aged 19–65 years. We identified differently expressed genes (DEGs) and differentially expressed proteins (DEPs) in adults compared to infants. The down-regulated genes in adults were associated with functional terms primarily related to sarcomeres, cellular maintenance, and metabolic, immunological and developmental processes. Thus, our study indicates age-related differences in the molecular signatures and associated functions of healthy skeletal muscle. Moreover, the findings assert that processes previously associated solely with aging are indeed part of development and healthy aging. Hence, combined findings of this study also indicate that age-dependent controls are crucial in muscle disease studies, as otherwise the comparative results may not be reliable.

ALOG/LSHs, a novel class of transcription factors: Evolutionarily conserved regulators of plant growth and development.

The ALOG/LSH group of proteins is highly conserved across plant lineage, starting from moss to higher flowering plants, suggesting their crucial role in the evolution and adaptation of land plants. The role of ALOG proteins is highly conserved in various developmental responses, such as vegetative and reproductive developmental programs. Their role in meristem identity, cotyledon development, seedling photomorphogenesis and leaf and shoot development has been relatively well established. Moreover, several key pieces of evidence suggest their role in inflorescence architecture and flower development, including male and female reproductive organs and flower colouration. Recent research has started to explore their role in stress response. Functionally, ALOG proteins have been demonstrated to act as transcriptional regulators and are considered a newly emerging class of transcription factors in plants that regulate diverse developmental and physiological processes. This review aims to stimulate discussion about their role in plant development and their role as transcription factors. It also aims to further unravel the underlying molecular mechanism by which they regulate growth and development throughout the plant lineage.

Real-time visualisation of developing chick embryos cultured in transparent plastic films from the blastoderm stage until hatching

Real-time visualisation of chick embryo development occurring inside an opaque eggshell is a major goal of developmental biology. This goal was partially achieved when 3-day-old embryos recovered from fertilised shelled eggs, preincubated in a conventional incubator, hatched after the egg contents had been transferred into shell-less embryo culture systems constructed of transparent plastic film placed in a plastic cup. However, the hatchable shell-less embryo culture systems described thus far cannot be used to visualise the dynamic morphological changes associated with the emergence of major organ systems during the first 3 days of embryo development, as the blastoderm area needs to be covered with an opaque membrane. Here, we present the development of a chick embryo culture system that enables omnidirectional real-time visualisation of the developing embryo, beginning from the newly laid blastoderm stage embryo until hatching.

Idiosyncratic gesture use in a mother-infant dyad in chimpanzees (Pan troglodytes schweinfurthii) in the wild

One promising method to tackle the question, “In which modality did language evolve?” is by studying the ontogenetic trajectory of signals in human’s closest living relatives, including chimpanzees (Pan troglodytes). Concerning gestures, current debates centre on four different hypotheses: “phylogenetic ritualization”, “social transmission through imitation”, “ontogenetic ritualization”, and “social negotiation”. These differ in their predictions regarding idiosyncratic gestures, making such occurrences a crucial area of investigation. Here, we describe a novel and potential idiosyncratic behaviour — ‘hand-on-eye’ — which was initially observed in one mother-infant dyad in a community of chimpanzees living in the wild. We systematically investigated the form, sequential organisation, intentionality, usage, function, and distribution of the behaviour over a five-year period. The results showed that ‘hand-on-eye’ was nearly exclusively deployed in a single mother-infant dyad, was accompanied by hallmarks of intentionality, and served to initiate or resume joint dorsal travel. Although the behaviour was observed once in each of three other mother-infant dyads, these lacked the same frequency and hallmarks of intentionality. ‘Hand-on-eye’ thus qualifies as an idiosyncratic gesture. The proposed developmental pathway gives support to both the “ontogenetic ritualization” and “social negotiation” hypotheses. It also stresses the crucial need for longitudinal approaches to tackle developmental processes that are triggered by unique circumstances and unfold over relatively long time windows.