Development Of Dental Diseases Research Articles

FLUORESCENT DIAGNOSTIC METHOD AS A TOOL FOR COMPREHENSIVE EVALUATION OF THE HYGIENE OF NATURAL TEETH AND FIXED DENTAL PROSTHESIS CONSTRUCTIONS

This study focuses on the critical need for an oral care, especially when using fixed bridge prostheses. The analysis and impact of insufficient care for oral hygiene on the development of dental diseases and the need to develop effective methods for its assessment have been carried out. The research consists of the improvement of fluorescent diagnostics in orthopedic dentistry, as well as, in the development of an index for the assessment of the hygiene of natural teeth and fixed denture structures based on an in vivo experiment. This aims to improve the diagnosis of the condition of the oral cavity in patients with fixed prostheses, contributing to the improvement of the quality of their orthopedic treatment. Materials and methods: The use of fluorescent diagnostics with Rhodamine G dye allows to identify the presence of dental deposits under the influence of ultraviolet light. The technique involves treating teeth and dentures with a solution that enhances the visibility of dental plaque under ultraviolet light. Research results: The obtained research data confirm the high efficiency of the fluorescent technique in the detection of dental deposits, both on natural teeth and on the surfaces of fixed prostheses. Digital analysis allows you to visualize the specific graphic characteristics of the biofilm, which facilitates the adaptation of an individual approach. Conclusions: The conducted study demonstrates that the improved fluorescent method was extremely effective in determining the state of oral hygiene, effective both for the analysis of natural teeth and fixed prostheses. The proposed method allows you to determine the hygienic condition in detail, makes a significant contribution to the prevention of dental diseases and improves the optimization of medical interventions. The technique is easy to use, and the corresponding software is available for dentists. Further expansion of observations for the use of this technique in patients with a different orthopedic spectrum of needs is expected.

INDEX-BASED ASSESSMENT ALGORITHM FOR ORAL HYGIENE IN WEARERS OF FIXED DENTAL BRIDGES

Introduction: The study focuses on the importance of maintaining oral hygiene, especially in wearers of fixed dental bridge. The impact of inadequate oral hygiene care on the development of dental diseases and the necessity for developing effective methods for its assessment are examined. Research objective: The aim of this study is to refine the fluorescent diagnostic method in orthopedic dentistry and to develop an index for assessing the hygienic status of natural teeth and fixed dental prostheses through an in vivo experiment. This method is intended to improve the diagnosis of oral conditions in wearers of fixed prostheses, ultimately enhancing the quality of their orthopedic treatment. Materials and methods: The study is based on the application of fluorescent diagnostics using Rhodamine B dye, which, when exposed to ultraviolet light, allows for the detection of dental deposits. The technique involves treating the surfaces of teeth and prostheses with a special solution that enhances the visibility of dental plaque under ultraviolet illumination. Research results: The experimental results demonstrated the high efficiency of the fluorescent method in detecting dental deposits both on the enamel of natural teeth and on the ceramic surface of fixed dentures. The digital analysis method allows for obtaining specific graphical indicators of the biofilm area, enhancing the relevance of an individual approach. Conclusions: Conclusions: The experimental study confirmed the high efficiency of the refined fluorescent method for assessing the hygienic status of the oral cavity, applicable to both natural teeth and fixed orthopedic constructions. This technique enables accurate assessment of hygiene status, facilitating effective prevention of dental diseases and optimization of treatment procedures. The method is convenient to use, and the software is accessible for clinicians. Further observations on the outcomes of this technique in patients with various orthopedic profiles are planned to expand its applicability.

АНАЛИЗ СТОМАТОЛОГИЧЕСКОЙ ЗАБОЛЕВАЕМОСТИ СРЕДИ ДЕТЕЙ ДОШКОЛЬНОГО ВОЗРАСТА ИЗ НЕПОЛНЫХ И РАСШИРЕННЫХ СЕМЕЙ

Introduction. This article is devoted to the study of the prevalence of dental diseases in preschool children living in incomplete and extended families. In the course of the study, children from both types of families were examined for dental caries and its complications, for non-caries dental diseases. While studying periodontal diseases, were distinguished inflammatory diseases, such as catarrhal and hypertrophic gingivitis, as well as localized periodontitis, when studying diseases of the oral mucosa, we highlighted diseases of the lips, tongue and oral mucosa. When studying dentoalveolarabnomalies, we identified both abnormalities of bite, dental arches and rows, and individual teeth. 
 The subject of research is dental diseases in preschool children.
 The goal of the study is to analyze dental morbidity in children from extended and incomplete families.
 Methodology. The study was conducted on the basis of the UniDent network of dental clinics, as well as in the Fairy Tale preschool commercial institution in Kazan and municipal preschool institutions of Tatarstan Republic. A total of 222 children from 217 incomplete families and 213 children from 199 extended families of both sexes participated in the voluntary study. Examination of children included examining the child and talking with parents or guardians, filling out medical records, determining the type of family, filling out an individual card in which identified dental diseases were recorded.
 The results of the study indicate a high prevalence of dental diseases among preschool children in TR. The progression of dental diseases is observed with the age of the child. Children from incomplete families are more susceptible to the occurrence and development of dental diseases, compared with children from extended families.
 Conclusions. The findings on the disparate prevalence of dental diseases in children from incomplete and extended families suggest the presence of family type influences, which may be useful in planning prevention methods and predicting dental outcomes in children from different family types.

Dental health of first-year students of the Novgorod State University

Goal and objectives: to study the dental health of 1st year students of the Novgorod State University. Materials and methods: questionnaire, literature review, study of statistical data on the Russian Federation, India, Egypt, and Pakistan. An analysis of the dental health of first-year Russian and foreign students of the Yaroslav-the-Wise Novgorod State University was carried out, and factors influencing dental health were identified. Official reports on the condition and composition of tap water in the city of Veliky Novgorod and tap water in Egypt, India, and Pakistan were studied. The standards for the content of fluorine and iodine in tap water in Veliky Novgorod, Egypt, India, and Pakistan were analyzed. The influence of changing climatic conditions, quality of nutrition, and the presence of bad habits and stress on the development of dental diseases in students was considered. The World Health Organization’s global oral health status report for 2022 in the Russian Federation, Egypt, India, and Pakistan was analyzed. A survey among students of the Novgorod University was conducted in order to evaluate water in the Novgorod Region in comparison with their homeland; the survey included general health questions with an emphasis on oral hygiene. What paste, brush, and cleaning method students used when brushing their teeth, and if they visited the dentist. What dental problems they had before and what issues they have now. Conclusions: To provide recommendations on oral hygiene, the choice of oral hygiene items and products, the diet taking into account the fluorine and iodine content in the region, and daily routine.

ORAL HEALTH STATUS OF CHALDREN WITH INFECTIOUS MONONUCLEOSIS IN THE PAST MEDICAL HISTORY

Dental caries and periodontal tissue diseases remain one of the most actual problems in pediatric dentistry. There is also a trend towards an increase in the prevalence of malocclusion. Dental morbidity in children in most cases develops under the influence of both local and general factors. Somatic pathology has a particularly significant impact on the development of dental morbidity in children, caused by metabolic disorders, which serves as the pathogenetic basis for the development of diseases. Viral infection, which is widespread among children and adults, plays a significant role in the development of dental diseases. In recent years, there has been an increase in the incidence of children with infectious mononucleosis caused by Epstein-Barr virus. Therefore, the assessment of oral health status in children with infectious mononucleosis will allow the development of effective means of preventing and treating diseases.
 Purpose. The purpose of the study is to assess the oral health status of children with infectious mononucleosis in the past medical history.
 Methods and Material. 240 children aged 6-15 years, including 112 children with infectious mononucleosis in the past medical history of (the main group) and 128 children of the comparison group, have been examined. The prevalence of dental caries, malocclusion and the condition of periodontal tissues were assessed in the examined children. The oral health status of children was determined in accordance with the recommendations of P.A. Leus (1991).
 Results. It has been found that in children with infectious mononucleosis in the past medical history, the prevalence of caries of permanent teeth, on average, was 73.68±3.04% with an intensity of 3.41±0.24 teeth, which was significantly higher than in children in the comparison group (65.06±2.98%; 2.53±0.22 teeth). Diseases of periodontal tissues were diagnosed in 69.8% of children in the main group and 48.7% in the comparison group. Among the periodontal diseases, chronic catarrhal gingivitis was the most common (62.7%). In children with infectious mononucleosis, malocclusion was detected, on average, in 86.36±4.22%, which is significantly higher than in the comparison group (69.23±4.27%). The oral health status of children with infectious mononucleosis aged 9 years was 70%, and 12 years 65%, and in the comparison group it was 80% and 76%, respectively.
 Conclusions. Thus, a decreased oral health status of children with infectious mononucleosis in the past medical history was found, especially in 12-year-old children, mainly due to the development of multiple dental caries and periodontal diseases. The identified dependencies indicate significant disorders of the protective mechanisms of the oral cavity, insufficiently effective oral hygiene and lack of preventive measures in children with infectious mononucleosis in the past medical history.

ANALYSIS OF MICROBIOCENOSIS OF ORAL FLUID IN PATIENTS WITH DEFECTS OF DENTITION, ZAKARPATTIA REGION RESIDENTS

Dental diseases are among the most frequent diseases of the human body. The microflora of the oral cavity is specific, unlike the microflora of other cavities both in composition, quantity, and functions. The resident microflora will play an important role both in maintaining the normal physiological state of the oral cavity and in the development of dental diseases.
 The aim of the study is to establish insemination with clinically significant microorganisms of oral fluid in patients with defects of dentition, Zakarpattia region residents.
 The species and quantitative composition of microbiocenosis of the oral fluid of 282 (94 men and 188 women) patients with dentition defects, residents of the Zakarpattia region, confirms the formation and progression of inflammatory and inflammatory-dystrophic changes in the periodontal tissues.
 In patients with bilateral end defects according to Kennedy, the predominance of anaerobic microflora was determined in the form of Klebsiella pneumonia (108), Prevotella spp. (7×105), Enterobacter spp. (102), Porphyromonas spp. (5×105), Staphylococcus epidermidis (104) and Staphylococcus aureus (1,2×103), Streptococcus pneumonia (108), Streptococcus viridans (103), Enterobacter spp. (102) and opportunistic microorganisms Candida albicans (104).
 Among patients with unilateral end defects according to Kennedy, Enterococcus faecalis was defined. (103), Staphylococcus epidermidis (102–103) and Staphylococcus aureus (105), Streptococcus pneumonia (105–107), Streptococcus viridans (102–5×105), Stahylococcus haemolyticus (102), Candida albicans (5×102) and Escherichia coli coli (109).
 In patients with included defects in the lateral areas according to Kennedy, there were cultured Klebsiella pneumonia (101–3.5×106), Prevotella spp. (4-5×101), Enterococcus faecalis (107), Peptostreptococcus micros (3.5×103–5×103), Staphylococcus haemolyticus (101–105) and Staphylococcus aureus (101–105), Streptococcus pneumonia (106–108 ), Streptococcus pyogenes (105–109), Candida albicans (102), Escherichia coli (107), Sarcina spp. (103) and Citrobacter spp. (104).
 Microbiocenosis of the oral fluid of the fourth clinical group with included defects in the frontal areas according to Kennedy is presented Lactobacillus acidophilus (108-1011), Streptococcu mitis (108-1011), Bifidobacterium (108-1010), Streptococcus salivarius (1081010), Streptococcus mutans (107-109), Streptococcus pneumonia (104 –108), Streptococcus sanguis (105–107).
 Therefore, in patients with dentition defects, the microflora of the oral fluid confirms the formation of inflammatory and inflammatory-dystrophic processes in the periodontal tissue due to the prevalence of anaerobic flora, while in the presence of inclusions in the frontal area, the prevalence of normal obligate microflora is diagnosed.

Dental diseases in patients with pathology of the gastrointestinal tract: a literature review

Annotation. The study aimed to analyze references in the scientific literature on hard dental tissue damage and periodontal and oral mucosal diseases in patients with concomitant gastrointestinal tract pathology. We used the bibliosemantic method to identify the status of the outlined issues and analysis of the scientific research data. Based on data from national and international published literature on the topic, the prevalence, severity and structurality of changes in the organs and tissues of the oral cavity associated with gastrointestinal tract diseases were evaluated. The review was carried out using data from scientific articles for the period of 2011-2022 with search words “Digestive System Diseases”, and “Diseases of the Oral Cavity” using the MESH subject headings: dental caries susceptibility, mouth diseases, gastrointestinal diseases, malabsorption syndromes, dental caries, tooth demineralization and others. The final analysis included 75 scientific articles corresponding to the purpose of the study. Data obtained from analysis of literary sources of recent years suggests that both local and general factors are involved in the process of occurrence and course of dental pathology with concomitant involvement of the gastrointestinal tract. The nature of their interaction will determine the peculiarities of the course of dental diseases. However, the lack of convincing data on the age-related features of the occurrence and development of dental diseases, as well as the inconsistency of the results regarding the significance of risk factors for the progress of comorbid conditions complicates the methods development of their treatment and prevention. The listed features of the course of dental diseases with a combined involvement of gastrointestinal disorders indicate the mandatory complexity of treatment, taking into account concomitant pathology.

Modifiable risk factors for the development and progression of periodontal tissue diseases

In this review, an analysis of modifiable risk factors for the development and progression of periodontal tissue diseases was carried out. The review highlights the data on the relationship of periodontal infection, tobacco smoking, diabetes mellitus, cardiovascular disease, drug-induced periodontal tissue damage, stress, and obesity with the risk of developing and progression of periodontal tissue diseases. A rather detailed analysis of the mechanisms of pathogenic influence of the microflora of the oral cavity, including in the subgingival plaque, was carried out. The mechanisms of the pathological effect of smoking on the state of periodontal tissues are described. Data on the effect of periodontal treatment on glycemic control in patients with diabetes mellitus are presented. The article emphasizes the importance of the presence of periodontal diseases as a risk factor for cardiovascular and cerebrovascular diseases, describes the mechanisms of the mutual influence of cardiovascular pathology and the inflammatory process in the periodontium. In addition, the article presents the possibilities of the iatrogenic effect of systemic drugs on the state of periodontal tissues. The data on the influence of stress loads on the development of dental diseases are presented. The review article describes in detail the mechanisms of influence of overweight and obesity on the risks of development and progression of periodontal tissue diseases. Based on the results of the scientific research publications presented in the article, it can be stated that not only the dentist, but also doctors of other specialties need to work to change the modifiable risk factors for the development and progression of periodontal diseases.

Formulation and Evaluation of Herbal Toothpaste

In today’s world, the herbal toothpaste that contains natural ingredients are more preferrable to be used than chemical based synthetic toothpaste. Herbal dental care products are always safe and effective which reduces and cure dental disorders. The objective of this research work was to formulate and evaluate the natural and safe herbal toothpaste which has anti-inflammatory, antimicrobial, antifungal and teeth whitening effect. In this formulation there would be the introduction of new flavoring agent i.e., Dill seed oil in conjugation with other herbal ingredients like fenugreek powder, neem powder, triphala powder, pomegranate peel powder, basil and guava leaves powder. The formulation was subjected to various evaluation tests like pH, spreadability, foamability, homogeneity, moisture and volatile content and tube inertness. Use of herbal toothpaste is the best way to protect the teeth and prevent the development of dental diseases. The Evaluation studies gives the satisfactory result. Thus, in present work, the lab made herbal toothpaste was found to be of best quality.

NEW PARADIGMS OF «DESIGN» OF THE ETIOPATHOGENETIC MODEL OF DENTAL DISEASES

Annotation. The statistical data of the World Health Organization on the prevalence of diseases of the oral cavity are presented. The relevance of studying the main mechanisms of the occurrence and development of dental diseases with the construction of a new research model is shown. The tactic of applying new data, associated cognitive principles and techniques is substantiated, which allows considering already known mechanisms and phenomena at the molecular, cellular, tissue levels, contributing to the expansion of ideas about the nature and nature of diseases. The aim of the work was to present new, promising methods to form an etiopathogenetic approach to curation of patients with dental caries and its complications. In accordance with the goal, analytical data on the effectiveness of diagnosing dental caries and its complications using immunoindicative methods are given; the prospects for further development of the direction are presented. It is emphasized that effective planning and implementation of dental treatment is based on competent diagnostics, which, at the present level of development, has the possibility of in-depth studies of the immune response, the morphology of cell interaction and the development of pathological processes. Possibilities of assessing the immune response by means of enzyme immunoassay, immunohistochemical analyzes are presented; consistent development of immunohistological interactions based on marker expression in target patients. Keywords: dental caries, complications, pulp, inflammation, cytokines, macrophage, markers, fibrosis.

THE ROLE OF MEDICAL AND SANITARY-HYGIENIC EDUCATION OF PARENTS IN MAINTAINING THE DENTAL HEALTH OF CHILDREN

Abstract: Assessment of the contribution of sanitary and hygienic education of parents in maintaining the dental health of children is an urgent task of practical health care. 70 children aged 4-6 years were examined. The hygienic condition of the oral cavity, the prevalence of caries and its intensity were evaluated. The parents of the examined children were surveyed in order to determine their medical and sanitary-hygienic literacy, as well as to clarify their attitude to conducting sanitary and educational work. The average value of the Fedorov-Volodkina index in children was 2.03±0.65, while one third of the examined patients showed an insufficient level of oral hygiene. The high prevalence (76%) and intensity (cp(h) 2.1±1.6) of caries were revealed. The relationship between the erroneous ideas of parents about the lack of the need for timely treatment of baby teeth with the unsatisfactory hygienic condition of the oral cavity, the prevalence and intensity of caries is established. The indicator of the relative risk of caries of baby teeth in children with the point of view of their parents that baby teeth can’t be treated was 1.36 (confidence interval 1.08-1.72, p<0.05), and a similar RR in their opinion that children's carious teeth should not be treated, they should be removed was 1.41 (confidence interval 1.14-1.76, p<0.05). The association of dental pathology in the examined children and the low level of medical and sanitary-hygienic knowledge of their parents turned out to be comparable with such a well-known association as frequent acute infectious diseases in children and the development of dental diseases in pediatrics, and is more pronounced than the relationship between the unfavorable course of pregnancy and diseases of the dental apparatus. It is necessary to expand measures for medical and sanitary-hygienic education of parents, the introduction of new forms of interaction between medical personnel and legal representatives of children in order to preserve dental health in pediatrics.

PHOTODYNAMIC THERAPY WITH METHYLENE BLUE IN CHILDREN WITH CEREBRAL PARALYSIS

Children with cerebral palsy suffer from dental diseases almost three times more often than healthy children. Risk factors that cause the development of dental diseases in children with CP include low rates of unstimulated salivation rate, acidity, increased saliva osmolarity and total protein concentration, which indicates a violation of hydration. The above factors contribute to the formation of acquired pellicles and biofilms due to increased bacterial agglutination. Ultimately, this contributes to the accelerated formation of plaque. There is a tendency to increase the concentration of hemoglobin and reduce the degree of oxygenation of oxyhemoglobin and, as a result, to increase the active forms of oxygen and nitrogen, which, in turn, have a toxic effect and lead to cell death. Photodynamic therapy (PDT) using photosensitizers (FS) improves blood filling and the degree of oxygenation in periodontal tissues, and also allows the elimination of bacterial biofilm. At the same time, opto-spectral diagnostics painlessly in the monitoring mode allows to identify and fix tissue areas with low hemoglobin oxygenation rates for precision photodynamic effects. PDT is a method used as a therapy for oral diseases of various origins, such as cancer, precancerous diseases, caries, periodontitis and gingivitis. Initially, methylene blue was the first dye used in medicine as an antiseptic, as well as the first tested and approved photosensitizer for antimicrobial photodynamic therapy. The use of photodynamic therapy with methylene blue as a photosensitizer can allow to deactivate inflammation and completely destroy bacteria in the biofilm, which will contribute to the functional normalization of gum tissue and restore oxygenation in the microcirculatory bed. The advantages of this method are antibacterial, anti-inflammatory and wound healing effects. The article presents the literature data on photodynamic therapy with the use of methylene blue in children, on the methodology and results of the use of this treatment technique in practice.

Dental Diseases And Their Development

The main content of the article is information about dental diseases and their types. Ways of development of dental diseases and measures for their elimination are given. Treatment is provided through folk medicine.

The dental plaque biofilm matrix.

The extracellular matrix is a critical component of microbial biofilms, such as dental plaque, maintaining the spatial arrangement of cells and coordinating cellular functions throughout the structure. The extracellular polymeric substances that comprise the matrix include carbohydrates, nucleic acids, proteins, and lipids, which are frequently organized into macromolecular complexes and/or are associated with the surfaces of microbial cells within the biofilm. Cariogenic dental plaque is rich in glucan and fructan polysaccharides derived from extracellular microbial metabolism of dietary sucrose. By contrast, the matrix of subgingival dental plaque is a complex mixture of macromolecules that is still not well understood. Components of the matrix escape from microbial cells during lysis by active secretion or through the shedding of vesicles and serve to anchor microbial cells to the tooth surface. By maintaining the biofilm in close association with host tissues, the matrix facilitates interactions between microorganisms and the host. The outcome of these interactions may be the maintenance of health or the development of dental disease, such as caries or periodontitis. The matrix affords microbial cells protection against chemical and physical insults and hinders the eradication of pathogenic dental plaque. Therefore, strategies to control the matrix are critical to maintain oral health. This review discusses recent advances in our understanding of the composition, origins, and function of the dental plaque matrix, with a focus on subgingival dental plaque. New strategies to control subgingival dental plaque based on targeting the biofilm matrix are also considered.

Characteristics of Trace Element Balance in Children with Pollution-Related Dental Diseases

Summary. Introduction: The necessity to assess, prevent and correct element-dependent diseases, increase the level of safety, predict and reduce the risk of exposures to industrial contaminants in the development of dental diseases in the child population is an important and promising area of modern hygiene. The objective of our study was to establish concentrations of trace elements in dental tissues of children exposed to various levels of industrial pollution. Materials and methods: We used atomic absorption spectrophotometry to measure 13 trace elements in extracted healthy and carious primary teeth in two groups of children aged 7–11. The first group consisted of 56 children living in the area with a significantly high level of anthropogenic burden (Caveraged total = 1.17 units) and a high prevalence of caries (86.9 %) while the second group consisted of 68 children living in the area with a relatively high level of anthropogenic burden (Caveraged total = 0.68 units) and a mean prevalence of caries (77.1 %) according to WHO criteria. Results: Our findings indicate that accumulation of toxic microelements in hard dental tissues of children in the first group was higher than in children of the second group: concentrations of lead, bismuth, cadmium and strontium were 2.6, 1.8, 2.5, and 1.2 times higher, respectively. We also observed higher accumulation of environmental toxicants in carious teeth compared to healthy ones. Thus, lead, cadmium and bismuth levels in carious teeth were 3.04, 1.2 and 3.13 times higher than in healthy teeth, respectively, while the level of strontium was, on the opposite, 2.5 times lower. Conclusions: The study revealed specific features of the interaction of essential trace elements in children in the form of antagonistic effects between cadmium and zinc, copper and manganese, and synergism in the content of copper, iron, nickel, zinc, and cobalt in healthy teeth, the mechanism of which is based on competition for a bond with a carrier substance and replacement of one element with another. In addition, we established that changes in the microelement balance in the hard tissues of healthy and carious teeth are not unidirectional in nature, thus reflecting complex interactions between the child’s organism and anthropogenic factors. The results proved that accumulation of toxic trace elements in hard dental tissues promotes caries development and causes a sharp decrease in the content of essential trace elements, especially chromium, manganese, iron, and copper. The study showed that hard dental tissues are a depot for lead, cadmium, bismuth, and strontium in children environmentally exposed to high levels of industrial chemicals. Features of developing an imbalance of biotic concentrations of essential elements and accumulating toxic microelements in children with pollution-related tooth pathology were also established.

Research of matrix metalloproteinases in therapeutic dentistry

This article the properties of matrix metalloproteinases, their classification, function and role in the development of dental diseases are presented. The analysis of matrix metalloproteinases has been performed, which proves their important role in the physiological and pathological processes of the oral cavity. The possibility of using matrix metalloproteinases as part of one of the methods for diagnosing diseases and using them to evaluate the effectiveness of treatment has been considered. The structure of different types of metalloproteinases, as well as a model of the catalytic domain of the MMP-8 molecule has been presented.

Computed tomographic findings of dental disease in domestic rabbits (Oryctolagus cuniculus): 100 cases (2009-2017).

To characterize the CT findings and epidemiological features of acquired dental disease in rabbits. 100 client-owned rabbits (Oryctolagus cuniculus). Medical records were searched to identify rabbits that underwent skull CT for any reason from 2009 to 2017. History, signalment, and physical examination findings were recorded. The CT images were reevaluated retrospectively for evidence of dental disease and graded according to a previously described system (from 1 [no evidence of disease] to 5 [severe dental disease]) for acquired dental disease in rabbits, and an overall (mean) grade was assigned. Descriptive analyses were performed. Factors were assessed for associations between dental disease grade and malocclusion stage. Common findings included premolar or molar tooth curvature in transverse (n = 100 rabbits) and sagittal (95) planes, apical elongation of premolar or molar teeth (99), sharp dental points (93), deformation of the mandibular canal (82), and periodontal ligament space widening (76). Acquired dental disease was classified as grade 1 (n = 2 rabbits), 2 (60), 3 (14), 4 (4), or 5 (20). Most CT findings were significantly correlated with each other. Agreement of grades was fair between left- and right-sided quadrants and between maxillary and mandibular quadrants. Age was associated with increasing dental disease grade and malocclusion stage (proportional ORs, 1.21 and 1.32/y, respectively). Fair agreement in disease grades between dental quadrant pairs indicated a degree of asynchrony in the development of dental disease. Findings suggested premolar or molar tooth curvature in a sagittal plane, subtle elongation at premolar or molar tooth apices, and mandibular canal deformation should be added to the grading system.

Effects of antibiotics at sub-minimal inhibitory concentrations on the morphology of Streptococcus mutans and Lactobacillus acidophilus

Sub-minimal inhibitory concentrations (sub-MICs) of antibiotics may have significant impact on the morphology and biochemical characteristics of target bacteria. This study aimed to examine the morphological changes to sub-MICs of antibiotics observed in bacterial cells from Gram-positive oral species, Streptococcus mutans and Lactobacillus acidophilus. The MICs for both amoxicillin and doxycycline were determined by broth dilution method. Individual cells of S . mutans increased in length after incubation with sub-MIC of amoxicillin, whereas its chain length increased in response to a sub-MIC of doxycycline. The lengths of individual bacterial cells of L. acidophilus decreased after incubation with sub-MICs of either amoxicillin or doxycycline. These results of suggest that sub- MICs of amoxicillin and doxycycline induce morphological changes in both S. mutans and L. acidophilus. Further studies are required to determine the significance of these findings for host-pathogen interactions and development of dental diseases.

Taking responsibility for the tooth: A semiotic and thematic analysis of oral health and disease in the TV show 'Embarrassing Bodies'.

Oral health and dentistry are seldom the subject of medical reality TV. This study investigates whether the dental segments within the British medical reality show, 'Embarrassing Bodies', may contribute to the anthropological understanding of oral health and social status, through semiotic and thematic analysis. This methodology involves close examination of both the visual and narrative themes within the programme. The show presents mouths afflicted by oral disease as traumascapes, the framing of which provides voyeuristic appeal. The portrayal of dental disease as negatively affecting human flourishing through shame and the inhibition of intimacy was common across the analysed cases. The key themes of intimacy and social distance; discipline, blame and personal responsibility; carnography; disciplining gaze and authority; and redemption and rebirth were identified through analysis. The cases also present a strong correlation between a lack of personal responsibility and the development of dental disease within the wider context of social class, with the dentist as a disciplining authority, enforcing professional and societal norms.

COMPARATIVE ANALYSIS OF DENTAL DISEASES AND ANTIOXIDANT DEFENSE OF MOUTH LIQUID IN CHILDREN FROM KRASNODAR HAVING CONGENITAL CLEFTS OF LIP AND/OR PALATE AND NEUROPSYCHIATRIC DISORDERS

The degree of antioxidant defense of mouth liquid is studied in children with cleft lip and/or palate (N=46) and in children with neuropsychiatric disorders (N=76) in connection with development of dental diseases. Control group is formed by somatically healthy children (N=60). Inspected children were subdivided into two subgroups dependant on their age: from 8 to 12 years (mixed bite) and from 13 to 15 years (permanent bite). All children were on clinical account in children dental office clinic of Kubanskii State Medical University and constantly lived in Krasnodar. In children with defects of maxillofacial area and with neuropsychiatric disorders in comparison with control, reduced indices of hygiene of mouth cavity, higher indices of distribution and intensity of caries, more heavy flow of periodontal diseases and increase of indices of «oxidative stress» in mouth cavity were observed. Obtained results evidence the display of «oxidative stress» on a background of low level of hygiene in two studied children groups with pathology, which by no means support the development of dental diseases (caries and periodontal diseases), and might be used in practical health protection for the prognosis of the flow of dental diseases.