Development Of Activity Research Articles

N6-Methyladenosine RNA Modification Regulates the Differential Muscle Development in Large White and Ningxiang Pigs.

N6-methyladenosine (m6A) is the most common modification in eukaryotic RNAs. Growing research indicates that m6A methylation is crucial for a multitude of biological processes. However, research on the m6A modifications in the regulation of porcine muscle growth is lacking. In this study, we identified differentially expressed genes in the neonatal period of muscle development between Large White (LW) and NingXiang (NX) pigs and further reported m6A methylation patterns via MeRIP-seq. We found that m6A modification regulates muscle cell development, myofibrils, cell cycle, and phosphatase regulator activity during the neonatal phase of muscle development. Interestingly, differentially expressed genes in LW and NX pigs were mainly enriched in pathways involved in protein synthesis. Furthermore, we performed a conjoint analysis of MeRIP-seq and RNA-seq data and identified 27 differentially expressed and m6A-modified genes. Notably, a typical muscle-specific envelope transmembrane protein, WFS1, was differentially regulated by m6A modifications in LW and NX pigs. We further revealed that the m6A modification accelerated the degradation of WFS1 in a YTHDF2-dependent manner. Noteworthy, we identified a single nucleotide polymorphism (C21551T) within the last exon of WFS1 that resulted in variable m6A methylation, contributing to the differing WFS1 expression levels observed in LW and NX pigs. Our study conducted a comprehensive analysis of the m6A modification on NX and LW pigs during the neonatal period of muscle development, and elucidated the mechanism by which m6A regulates the differential expression of WFS1 in the two breeds.

FgUbiH Is Essential for Vegetative Development, Energy Metabolism, and Antioxidant Activity in Fusarium graminearum

Fusarium head blight in wheat is mainly caused by Fusarium graminearum and results in significant economic losses. Coenzyme Q (CoQ) is ubiquitously produced across organisms and functions as a hydrogen carrier in energy metabolism. While UbiH in Escherichia coli serves as a hydroxylase in CoQ biosynthesis, its role in phytopathogenic fungi is not well understood. This study explored the role of the hydroxylase FgUbiH in F. graminearum. Using a FgUbiH deletion mutant, we observed reduced hyphal growth, conidial production, germination, toxin synthesis, and pathogenicity compared to the wild-type. A transcriptome analysis indicated FgUbiH’s involvement in regulating carbohydrate and amino acid metabolism. Deletion of FgUbiH impaired mitochondrial function, reducing adenosine triphosphate synthesis and increasing reactive oxygen species. Additionally, genes related to terpene skeleton synthesis and aldehyde dehydrogenase were downregulated. Our results underscore the importance of FgUbiH in F. graminearum’s growth, toxin production, and energy metabolism, aiding in the development of strategies for disease management.

Comparative study of the reproductive activity of Calliptamus barbarus (Costa, 1839) and Calliptamus wattenwylianus (Pantel, 1836) in a subhumid bioclimatic region

The reproductive activity of Calliptamus barbarus and Calliptamus wattenwylianus was investigated to understand population dynamics and reproductive potential in a subhumid bioclimatic zone. Specimens were collected from July to October and dissected to assess ovarian development and egg-laying activity. The number of ovarioles, determined at birth, ranged from 62 to 67 for C. wattenwylianus and 48 to 58 for C. barbarus. Higher fertility was observed in C. wattenwylianus, with egg production increasing during the first oviposition for both species. The average reproductive yield was notable for C. barbarus (85.99%) and C. wattenwylianus (86.56%). Oocyte resorption occurred mainly during drought (August-September) and cold periods (October-November). C. wattenwylianus females reached maturity earlier, beginning in August, compared to C. barbarus. Egg production began in July for C. wattenwylianus and in September for C. barbarus. Oocyte retention rates were 5% for C. barbarus and 9% for C. wattenwylianus. These findings provide insights into reproductive patterns in North African Orthoptera, with implications for pest control in subhumid regions.

LIN28 upregulation in primary human T cells impaired CAR T antitumoral activity.

LIN28, a highly conserved RNA-binding protein that acts as a posttranscriptional modulator, plays a vital role in the regulation of T-cell development, reprogramming, and immune activity in infectious diseases and T-cell-based immunotherapies. LIN28 inhibit the expression of let-7 miRNAs, the most prevalent family of miRNAs in lymphocytes. Recently it has been suggested that let-7 enhances murine anti-tumor immune responses. Here, we investigated the impact of LIN28 upregulation on human T cell functions, focusing on its influence on CAR T cell therapy. LIN28 lentiviral transduction of human T cells led to a stable expression of LIN28 that significantly downregulated the let-7 miRNA family without affecting cell viability or expansion potential. LIN28 overexpression maintained human T cell phenotype markers and functionality but impaired the antitumoral cytotoxicity of NKG2D-CAR T cells both in vitro and in vivo. These findings highlight the intricate relationship between LIN28/let-7 axis and human T cell functionality, including in CAR T cell therapy.

Physico-chemical characteristics and quality assessment of soil in parts of the Banganga Basin in northeastern Rajasthan, NW India.

Soil is a vital natural resource that has a bearing on any developmental activity. A database comprising primary physicochemical characteristics and soil suitability would assist the planners in devising an appropriate land use policy and any intervention strategy for soil quality improvement. The physicochemical characteristics of soils in parts of the Banganga Basin, located in the semi-arid terrain of NW India were evaluated mainly through surface samples and sub-surface ones wherever sections were exposed in stream banks. The soil in the studied terrain is a Quaternary age, pale brown to dark brown and occasionally red, alluvial soil. Sandy loam is the dominant soil type, while sandy soil and loamy sand types also occur. The soil maintains a depth-wise consistency in physicochemical characteristics. The average soil pH value is 8.55, indicating a slightly alkaline and healthy soil. The soil has a high available nitrogen content (0.53 to 2.24%), adequate available phosphorus (23.58 and 62.18mg/kg), and low potassium (22.5 and 200mg/kg) contents. The organic carbon, Mg, and Ca levels are generally on the lower side. A consistency in overall soil characteristics is evident in the narrow range of the Soil Quality Index, varying between 10 and 14, and soil can be categorized into three categories with overlapping soil qualities.

The Impacts of Coaching on Innovative Food Business Performance: A Study of Indonesian Entrepreneurship Development Program

Business coaching has emerged as a prominent tool for enhancing organizational performance and individual development. Relying on the Goal-setting theory, this article uses qualitative method to provide an empirical study about the impacts of the GROW (Goals, Reality, Options, and Will) model of business coaching on product development, managerial improvement, and fulfilment of legal aspects on top of financial aspects for Indonesian micro and small food businesses. The study was conducted based on the results of the Indonesian Food Innovation (IFI) coaching program; an entrepreneurship development activity initiated by the Indonesian Ministry of Industry. The data in this study was gathered from the tracery study carried out by the IFI committee. Conducting an experiment method using comparative of pre- and post-activities, this research compares the participants’ business performance before vs. after coaching intervention, this study finds evidence of the significant positive impacts of business coaching on enhancing business performance, such as sales value and business valuation. The success of an entrepreneurship development program is determined by the insight into vital issues that are specifically often faced in a particular industry. Therefore, entrepreneurship development programs need to be customized according to the industry.

Fighting the Machine: Co-constructing Team Based Evaluation for Non-Formal Learning.

Museum educators increasingly face demands to evaluate the ‘value’ of non-formal learning (NF-L). This paper offers a unique international and multi-educator perspective on how informants from Portugal, Italy and the United Kingdom navigated these demands. Analysis of interview data highlighted that, although working in three different countries, most of these educators had experienced evaluation as accountability (and disciplinary) focused; employing methodologies inappropriate for evaluating NF-L and rarely team based. Drawing on a composite theoretical framework, these data led to co-constructing the Team Based Evaluation (TBE) model. Two case studies map how TBE was enacted and recommendations concerning organizational change are made. The paper concludes that, whilst set within the museum education space, TBE can be applied across evaluation contexts and micro and macro scales. Locally, TBE can mediate rich evidence and develop team working practices. Nationally and internationally, it can contribute to resetting evaluation from an accountability and disciplinary ‘machine’ to a dialogic, democratic and developmental activity.

Chemical synthesis of a trisaccharide fragment of a fucosylated glucuronomannan isolated from brown algae

Fucose is abundant in marine organisms, primarily as fucoidan and fucosylated chondroitin sulfate. Our previous study characterized a sulfated and fucosylated glucuronomannan (SFGM) isolated from marine brown algae, exhibiting notable antiviral activity and potential for drug development. Herein, a trisaccharide fragment of this SFGM in the form of [α-L-Fuc(2, 4-diS)]-(1→3)-α-D-Man-(1→4)-α-D-GlcA-OMP was synthesized for the first time. Stereoselective formation of the α-1,2-cis-fucosidic bond was achieved by leveraging solvent effects. The sulfated trisaccharide as the final product, featuring a 2,4-diS-fucose unit and the p-methoxyphenyl group as a pendant at the anomeric position, was successfully accomplished. The synthetic strategy developed in this study offers a foundation for the pharmaceutical development of these SFGM oligosaccharides.

Occupational exposure assessment of heavy metals among construction workers in Rawalpindi Pakistan

Construction is one of the primary drivers of development and economic activity in Pakistan. Many activities are performed at the construction site including cement mixing, carpentry, and painting. Workers working in these activities are exposed to toxic heavy metals. Due to exposure to these heavy metals, subjects are at high risk of developing diseases mainly respiratory diseases. Human hair and nail samples of construction workers in Rawalpindi, Pakistan were collected to assess the heavy metals. Heavy metals such as Al, As, Cd, Cr, Ni, K, Pb, and Zn were analyzed in the hair and fingernails of the construction workers. To determine the level of selected heavy metals, Inductively Coupled Plasma optical emission spectrometry was used. Results revealed that Al was the highest with a mean concentration of 7.54 μg/g in all three groups. Whereas Cd was below the detection limit. Heavy metal concentration was high in hair (1.77 μg/g) in two groups including cement mixing and carpentry. However, in the carpentry group heavy metal concentration was high in nails (1.19 μg/g). Additionally, the workers of the carpentry group have higher metal concentrations when compared to the other two groups and this group was at high risk of toxic metal exposure. After the correlation analysis, the results revealed that age was significantly correlated to body burden of metals (r = 0.496, p ≤ 0.05). Moreover, some of the heavy metals in hair and nails were significantly correlated with each other. Since all groups, especially carpenters, show high levels of heavy metals, there is a need for regular monitoring of heavy metals at construction sites. Most importantly, the implementation of training programs is needed to raise awareness among construction workers.

Evaluation of the Energy Management System in Water and Wastewater Utilities in the Context of Sustainable Development—A Case Study

Energy management in enterprises is an important issue in the context of improving energy efficiency, energy use, and energy consumption. This is consistent with the Sustainable Development Goals. The purpose of this study was to evaluate the energy management system of water and wastewater utility in the context of sustainable development based on the opinions of managers and employees. The results indicate the involvement of the surveyed enterprise in energy management system development activity. This demonstrates the orientation of the surveyed enterprise to support activities to improve energy performance in line with the implementation of sustainable development. The added value is that the developed research tool can be used in studies of other enterprises to assess the level of energy management.

The consequence of the digital economy on energy efficiency in Chinese provincial and regional contexts: Unleashing the potential

The digital economy, driven by advanced technologies, is becoming a new force for economic growth and sustainable development. This study investigates how the digital economy influences energy efficiency and its spatial spillover effects across 30 Chinese provinces from 2011 to 2020 using spatial econometric models and Moran's index analysis. While prior research focused on industrial value and micro-mechanisms, this work examines geographical impacts and regional synergies overlooked previously.The findings reveal urbanization and digital economy growth significantly enhance energy efficiency. However, the effects of economic structure, education, infrastructure development, and patent activity exhibit more nuanced patterns. Notably, the spatial spillover effects of the digital economy on energy efficiency operate through a "market + geographical comprehensive mechanism" rather than direct geographic transmission between neighbors. This study contributes to the literature by uncovering the complex relationships between the digital economy, technological innovation, industrial structure, and energy efficiency while accounting for spatial dynamics. The results underscore the importance of focused policies supporting sustainable energy practices based on regional contexts. By providing insights into the intricate interplay of factors influencing energy efficiency, this research informs strategies for optimizing energy utilization and transitioning towards a sustainable, secure, and affordable energy future aligned with net-zero objectives.

Air and Surface Temperatures Differently Drive Terrestrial Carbon and Water Cycles in the High Latitudes

AbstractHigh‐latitude vegetation experience different temperatures than the ambient air temperature. While lacking a regional plant temperature product, we drove the dynamic ecosystem model, LPJ‐GUESS, with widely used ERA5‐land surface temperature (Tsurf, at radiative equilibrium) and air temperature to understand ecosystem process responses to these two temperatures. We show that tundra plants' growth is stimulated by warmer Tsurf in the summer, but in the boreal forests, colder Tsurf in the non‐summer months constrains leaf development and enzyme activity the following growing season. Tsurf drives higher productivity of tundra plant individuals but leads to less productive individuals in the boreal forest, although with compensatory changes (almost 68%) in vegetation structure. We demonstrate the importance of forcing temperature in simulating high‐latitude ecosystem processes and call for a community effort to measure plant temperatures across canopy heights and seasons to reduce uncertainties in estimating high‐latitude plant responses and feedback to climate.

A-090 Performance of GFAP and UCH-L1 Biomarkers for Traumatic Brain Injury in the ARCHITECT i1000SR TBI Test

Abstract Background Traumatic Brain Injury (TBI) has been recognized as an important cause of death and disability and is a growing public health problem. An estimated 69 million people globally experience a TBI annually. The vast majority (80%) of patients evaluated for TBI in the Emergency Department will receive head CT scans, however less than 10% will have findings of acute traumatic abnormalities. This highlights the need for objective, rapid, accurate tools to help clinicians evaluate and triage patients with suspected TBI and reduce unnecessary radiation exposure. Blood based biomarkers such as glial fibrillary acidic protein (GFAP) and ubiquitin C-terminal hydrolase L1 (UCH-L1) have shown utility to predict acute traumatic intracranial injury on CT scan after TBI. Methods The ARCHITECT TBI test is a panel of in vitro diagnostic chemiluminescent microparticle immunoassays for the measurement of GFAP and UCH-L1 in plasma and serum. A method comparison study was performed to compare the GFAP and UCH-L1 assays on ARCHITECT i1000SR to the FDA cleared Alinity i assays. Performance characteristics such as detection limits, imprecision, linearity, and measuring interval were established following CLSI guidance. Results The analytical measuring interval (AMI) extends from the limit of quantitation (LoQ) to the upper LoQ and is determined by the range that demonstrates acceptable performance for linearity, imprecision, and bias. The AMI is 6.1 to 42,000 pg/mL for GFAP and 26.3 to 25,000 pg/mL for UCH-L1. Within-laboratory imprecision ranged from 2.2 to 6.2% CV for GFAP and 2.2 to 4.5% CV for UCH-L1. The Overall Reproducibility (includes repeatability, between-run, between-day, between-instrument variance components) results ranged from 2.7 to 6.8% CV for GFAP and 2.4 to 3.9 %CV for UCH-L1. The method comparison study evaluated 123 specimens across the range of 6.5-32,548.6 pg/mL for GFAP, and 51.5-24,127.7 for UCH-L1. The correlation coefficients were 1.0, with a slope of 1.03 and 1.06, an intercept of -0.6 and -6.0, for GFAP and UCH-L1, respectively. The results support use of the established GFAP and UCH-L1 cutoffs and clinical performance findings in a multi-site pivotal study which enrolled 1899 mild TBI (Glasgow Coma Scale score 13 to 15) subjects. 116 of the 120 specimens with a positive CT scan had a positive TBI interpretation (Sensitivity 96.7%). Of the 1779 subjects with negative CT scan results, 713 had a negative TBI interpretation (Specificity 40.1%). The negative predictive value (NPV) of the test was 99.4% (713/717). Conclusions The ARCHITECT TBI test performance was evaluated compared to the Alinity i TBI test across a broad concentration range of GFAP and UCH-L1. The results support equivalence of the ARCHITECT TBI test to the Alinity i TBI test. The established cutoffs were evaluated in a prospective cohort of mild TBI subjects and demonstrated high sensitivity and NPV relative to head CT scan results, supporting the utility to rule out the need for CT scan in mild TBI subjects presenting to the emergency department. Funding This work is in collaboration with the US Army Medical Materiel Development Activity, US Army Medical Research and Development Command CRADA 20-1266-CRA.

Exploring research topics and trends in early childhood education using structural topic modeling

Early childhood education plays a key role in fostering child development and preparing children for school and life. The aim of the present paper is to give an overview of research topics in early childhood education research based on the analysis of 39,926 scientific articles published between 2000 and 2021 and to explore research trends over time. Therefore, a structural topic modelling approach was used. The analyses show a strong increase in publication activity over the last years as well as the diversity of research topics, which provide important insights for achieving the UN Sustainable Development Goals in various areas (cultural diversity, design of inclusive learning environments, educational institutions, professional development issues, educational policy and reforms, etc.). Beside topics like cultural diversity, and inclusive learning environments more specific research topics, for example self-regulation, executive functions, numeracy, language development and physical activity have been increasingly addressed in publications over the years.

Development and Implementation of a Geriatric Oncology Interdisciplinary Case-Based Educational Intervention for Cancer Care Providers.

To develop and implement a continuing professional development (CPD) activity focused on geriatric assessment (GA) in oncology for oncologists and geriatricians. We evaluated the impact of this activity on knowledge, skills, and performance regarding GA in oncology, as well as its feasibility and acceptability. We included teams composed of an oncologist and a geriatrician working in Mexico. Curriculum content was selected from geriatric oncology guidelines. We used Project Extension for Community Healthcare Outcome (ECHO)'s model to create a 12-week online course. A one-group pretest post-test quasi-experimental design was used to evaluate the intervention's effectiveness. At baseline, participants answered a multiple-choice knowledge assessment, a survey on self-perceived competence in GA, and an adaptation of the Association for Community Cancer Centers Geriatric Oncology Gap Assessment Tool, evaluating self-perceived performance in conducting geriatric interventions. These assessments and a satisfaction questionnaire were also completed postintervention. Baseline and postintervention scores were compared using paired t-tests. We included 40 participants (20 oncologists and 20 geriatricians). Median attendance was 10 sessions (range 2-12). Thirty-eight participants completed the satisfaction questionnaire, with a median score of 10/10 (range 8-10). The mean baseline and postintervention knowledge scores were 59.5 ± 12.8 and 74.4 ± 9.7, respectively (P < .001, effect size 1.14). The mean baseline and postintervention competence scores were 6.42 ± 2.5 and 9.02 ± 0.8, respectively (P < .001, effect size 1.03). The mean baseline and postintervention performance scores were 2.58 ± 0.65 and 3.29 ± 0.5, respectively (P < .001, effect size 1.64). A CPD activity for oncologists and geriatricians on the basis of the Project ECHO model was feasible and acceptable, leading to increased knowledge, competence, and performance in geriatric oncology. This could represent a novel method for increasing the geriatric competence of the cancer care workforce in Latin America and globally.

Critical analysis of Rwanda’s Digital skills and entrepreneurship training toward solving youth unemployment

This study critically examines the role of digital skills and entrepreneurship training in addressing the persistent issue of youth unemployment in Rwanda. The paper primarily anchors on the Human Capital Theory and Entrepreneurship Theory to provide a solid theoretical framework, tying together education, skill development, and entrepreneurial activity as vital drivers of productivity, job creation, and economic development. By employing a qualitative methods approach that utilises qualitative stakeholder interviews, this research evaluates the effectiveness of the various policies and programs aimed at fostering digital and entrepreneurship skills to young people in Rwanda, to promote employment. The research unveils that these programs hold significant potential in equipping the Rwandan youth with relevant skills and nurturing an innovative and entrepreneurial culture. However, it also highlights key areas requiring attention: the lack of robust follow-up mechanisms and the need for continued support post-training. It advocates for policymakers and program implementers to prioritize these aspects to maximize their initiatives' impact. The implications of this study extend beyond Rwanda's borders as countries worldwide grapple with similar challenges. Therefore, the insights gleaned from Rwanda's approach could guide other nations in leveraging digital skills and entrepreneurship training to combat youth unemployment in the digital age. It concludes by recommending future research directions, including maintaining program relevance in the ever-evolving digital landscape and instituting more effective support mechanisms for venture sustainability.

STUDY OF PHYSICOCHEMICAL AND BIOLOGICAL PROPERTIES OF A NEW BISPIDINE WITH A MORPHOLINE FRAGMENT

The experimental results obtained during the work enrich modern medicinal chemistry with new information about the development and local anesthetic activity of a promising class of chemical compound - O-benzoyloxime of bispidine. The discovered new properties of the molecule can serve as the basis for the creation of a new domestic safe local anesthetic drug that is effective in models of infiltration and conduction anesthesia and has low toxicity. The aim of this work is to synthesize and study the physicochemical and biological properties of a new morpholino-substituted bispidine derivative. Results and discussion. 3-(3-(Ethan-1-yloxy)propyl)-7-[3-(morpholine-4-yl)propyl]-3,7-diazabicyclo[3.3.1]nonan-9-one was constructed in 56.9% yield. Oximation under the influence of a powerful oximylating agent led to the oxime. O-Benzoyloxime was synthesized by benzoylation of the oxime. The complex of O-benzoyloxime with β-cyclodextrin - LA-180 was studied for local anesthetic activity and acute toxicity. Conclusion. It was established that LA-180 manifested itself as a highly active compound in a series of experiments studying infiltration and conduction anesthesia. The results of a study of acute toxicity after a single subcutaneous administration to white outbred mice showed that LA-180 (925 mg/kg) is a low-toxic compound in comparison with standard drugs such as trimecaine, lidocaine and novocaine.

The geometry of conchiferan shell evolution: origins of coiling and bivalved morphologies

ABSTRACT The unifying theme of conchiferan shell morphology, including near-logarithmic expansion of a coiling cone, has led to the development of numerous descriptive mathematical models. Here, we develop and demonstrate a simple geometric model, based on minimal assumptions of ontogenetic development and mantle activity, that seeks to explain several fundamental features, including the origin of coiling, lateral compression and both pseudobivalved and true bivalved morphologies in the Diasoma. Coiling is hypothesized to have been facilitated by a genetically controlled asymmetry in the rate of mineralization around the growing margin of early Cambrian univalved taxa that adhered to an epibenthic life mode. Coiling would have been advantageous, and still is, for increase of size, internal volume expansion and protection against hydrodynamic and biotic forces. Three hypotheses are tested for the origin of Rostroconchia and Bivalvia. The first is an existing hypothesis of descent from laterally compressed helcionellid taxa. The other two are novel hypotheses, the first involving a bifurcation of the mineralizing asymmetry of the shell field along the anterior–posterior axis of symmetry, and the second proposes a bifurcation of the shell field itself, rendering each bivalve valve homologous with the univalve shell. Both hypotheses generate model shells consistent with rostroconch and bivalve morphologies respectively, and it is suggested that the proliferation of laterally compressed conchiferans in the late Cambrian is at least partially a result of multiple independent, and evolutionarily convergent invasions of organic and algae rich soft sediment environments.

RESEARCH &amp; DEVELOPMENT OF P2O5 DETERMINATION METHOD IN PHOSPHATE FERTILIZERS AND ITS VALIDATION

The validation of chemical measurement for Phosphorus determination in fertilizers followed a chain of experimental procedures to ascertain the performance characteristic (PC), connecting Precision, accuracy, reproducibility, recovery, linearity, limit of detection and of quantification. Performance characteristics are accomplished to ensure that analytical method fits the purpose of particular testing. This method was conducted in three stages those were preparation, testing and data processing. The standard test method for determination of P2O5 content in fertilizers used for regulatory purpose is somewhat lengthy and time taking. A modification was done to the standard method to create a more efficient approach that meets validation criteria and is suitable for analyzing advisory fertilizer samples. During the year 2023-24, comprehensive research and development activity were carried out at Soil and Water Testing Laboratory (SWTL) for Research, Faisalabad and analytical data was generated. The obtained results were processed statistically and compared with the acceptance requirements. The coefficient of determination (R2), precision and accuracy/recovery percentage respectively were 0.9996, 0.3-1.10% and 98.01-102.34%, while LOD and LOQ were 0.84 and 2.25%. Based on the results the study concluded that the modified test method applied to determine P2O5was appropriate for its intended use based on the acceptance criteria of performance characteristics set forth in the validation protocol and can be applied as routine method at laboratory.

Prevalence of Balance Impairment in Physically Active and Sedentary Lifestyle Children Aged 7-10 Years

Background: Balance impairment can significantly affect motor development in children, with lifestyle factors such as physical activity playing a crucial role. Physically active children may develop better balance than those leading sedentary lifestyles.Objective: To evaluate the prevalence of balance impairment in physically active and sedentary children aged 7-10 years.Methods: A cross-sectional study was conducted on 377 children aged 7-10 years from schools in Sheikhupura, Pakistan. The participants were divided into physically active (n=189) and sedentary (n=188) groups based on the International Physical Activity Questionnaire for Children (IPAQ-C). Balance was assessed using the Pediatric Balance Scale (PBS), a 14-item tool with a maximum score of 56. Data were analyzed using IBM SPSS version 25, with chi-square tests to determine statistical significance (p&lt;0.05).Results: In the physically active group, 79.4% (n=150) had good balance scores, while 67.6% (n=127) in the sedentary group showed moderate balance impairment. The difference between the two groups was statistically significant (p&lt;0.001).Conclusion: Physically active children demonstrated better balance compared to their sedentary peers, underscoring the importance of physical activity in childhood development