Development-related Factors Research Articles

Scenario-based statistical analysis for PM2.5 concentration: A case study of Seoul, South Korea

Rapid urban expansion and active economic development significantly contribute to increased levels of PM2.5, which degrades the urban environment. This study aims to establish a foundation for practical policy guidance on the impact of urban factors on PM2.5 concentrations in Seoul, South Korea. To achieve this objective, we utilized various urban factors, including socio-demographic data, three-dimensional urban geometry, land use, and traffic volume, in conjunction with PM2.5 concentrations estimated by satellite images. According to our statistical models, all urban development-related factors, except for Forest and Open Space (FOS), showed a statistically significant increase in PM2.5 concentration. However, FOS demonstrated an effective mitigation capability, with a 1% increase in FOS corresponding to an approximate 5% decrease in PM2.5 levels. In addition, five scenario analyses based on statistical results were conducted to evaluate the potential reduction of PM2.5 and associated spatial implications resulting from the removal of batching plants, decreased traffic volume, and the substitution of transportation network with green spaces in Seoul. They highlighted the importance of reducing traffic and increasing green spaces in order to lower PM2.5 levels. The findings suggest that a multifaceted strategy is necessary to develop efficient policies for mitigating PM2.5 for a sustainable environment.

Role of regulatory T cells in mouse lung development.

Regulatory T cells (Tregs) constitute a specialized subset of T cells with dual immunoregulatory and modulatory functions. Recent studies have reported that Tregs mediate immune responses and regulate the development and repair processes in non-lymphoid tissues, including bone and cardiac muscle. Additionally, Tregs facilitate the repair and regeneration of damaged lung tissues. However, limited studies have examined the role of Tregs in pulmonary development. This study aimed to evaluate the role of Tregs in pulmonary development by investigating the dynamic alterations in Tregs and their hallmark cellular factor Forkhead box P3 (Foxp3) at various stages of murine lung development and establishing a murine model of anti-CD25 antibody-induced Treg depletion. During the early stages of murine lung development, especially the canalicular and saccular stages, the levels of Treg abundance and expression of Foxp3 and transforming growth factor-β (TGF-β) were upregulated. This coincided with the proliferation period of alveolar epithelial cells and vascular endothelial cells, indicating an adaptation to the dynamic lung developmental processes. Furthermore, the depletion of Tregs disrupted lung tissue morphology and downregulated lung development-related factors, such as surfactant protein C (SFTPC), vascular endothelial growth factor A (VEGFA) and platelet endothelial cell adhesion molecule-1 (PECAM1/CD31). These findings suggest that Tregs promote murine lung development.

Advances in Physalis molecular research: applications in authentication, genetic diversity, phylogenetics, functional genes, and omics.

The plants of the genus Physalis L. have been extensively utilized in traditional and indigenous Chinese medicinal practices for treating a variety of ailments, including dermatitis, malaria, asthma, hepatitis, and liver disorders. The present review aims to achieve a comprehensive and up-to-date investigation of the genus Physalis, a new model crop, to understand plant diversity and fruit development. Several chloroplast DNA-, nuclear ribosomal DNA-, and genomic DNA-based markers, such as psbA-trnH, internal-transcribed spacer (ITS), simple sequence repeat (SSR), random amplified microsatellites (RAMS), sequence-characterized amplified region (SCAR), and single nucleotide polymorphism (SNP), were developed for molecular identification, genetic diversity, and phylogenetic studies of Physalis species. A large number of functional genes involved in inflated calyx syndrome development (AP2-L, MPF2, MPF3, and MAGO), organ growth (AG1, AG2, POS1, and CNR1), and active ingredient metabolism (24ISO, DHCRT, P450-CPL, SR, DUF538, TAS14, and 3β-HSB) were identified contributing to the breeding of novel Physalis varieties. Various omic studies revealed and functionally identified a series of reproductive organ development-related factors, environmental stress-responsive genes, and active component biosynthesis-related enzymes. The chromosome-level genomes of Physalis floridana Rydb., Physalis grisea (Waterf.) M. Martínez, and Physalis pruinosa L. have been recently published providing a valuable resource for genome editing in Physalis crops. Our review summarizes the recent progress in genetic diversity, molecular identification, phylogenetics, functional genes, and the application of omics in the genus Physalis and accelerates efficient utilization of this traditional herb.

Effects of host plant on the development and reproduction of Agrotis ipsilon (Lepidoptera: Noctuidae) on horticultural crops

One of cosmopolitan pest, Agrotis ipsilon (Lepidoptera: Noctuidae), causes serious economic damages in horticultural crops. This pest is difficult to manage and causes irreversible damage because its larvae stay in the ground at day and cut the plant stems at night. Thus, this study compared the host fitness of A. ipsilon among nine major horticultural crops in Korea. Among the nine crops, the population of A. ipsilon failed to complete its development in spinach, cucumber, melon, and kidney bean. The host effects on development and reproduction of A. ipsilon were further investigated in the remained five crops (i.e., napa cabbage, soybean, perilla, corn, and pepper). Host plants significantly (P < 0.05) affected the development-related factors (i.e., developmental time, survivorship, and weight) of A. ipsilon eggs, larvae, and pupae. They also affected the adult reproduction-related factors including preoviposition period, oviposition period and number, and longevity except for the prepupa stage. A positive relationship was found between biological factors (i.e., development- and reproduction-related factors). Among the nine crops in this study, napa cabbage showed the highest suitability for the A. ipsilon populations. These findings in this study would be helpful to understand the ecology and develop the management tactics of A. ipsilon in horticultural crops.

The representation of women on Australian clinical practice guideline panels, 2010-2020.

To assess the composition by gender of Australian clinical practice guideline development panels; to explore guideline development-related factors that influence the composition of panels. Survey of clinical guidelines published in Australia during 2010-2020 that observed the 2016 NHMRC Standards for Guidelines, identified (June 2021) in the NHMRC Clinical Practice Guideline Portal or by searching the Guideline International Network guidelines library, the Trip medical database, and PubMed. The gender of contributors to guideline development was inferred from gendered titles (guideline documents) or pronouns (online biographies). The overall proportion of guideline panel members - the guideline contributors who formally considered evidence and formulated recommendations (ie, guideline panel chairs and members) - who were women. Of 406 eligible guidelines, 335 listed the names of people who contributed to their development (82%). Of 7472 named contributors (including 511 guideline panel chairs [6.8%] and 5039 guideline panel members [67.4%]), 3514 were men (47.0%), 3345 were women (44.8%), and gender could not be determined for 612 (8.2%). A total of 215 guideline panel chairs were women (42.1%), 280 were men (54.8%); 2566 guideline panel members were men (50.9%), 2071 were women (41.1%). The proportion of female guideline panel members was smaller than 40% for 179 guidelines (53%) and larger than 60% for 71 guidelines (21%). The median guideline proportion of female panel members was smaller than 50% for all but two years (2017, 2018). The representation of women in health leadership roles in Australia does not reflect their level of participation in the health care workforce. In particular, clinical guideline development bodies should develop transparent policies for increasing the participation of women in guideline development panels.

PD47 Associations Of Orphan Designation And Other Drug Development-Related Factors On Rollout Times And Health-Technology-Assessment Recommendations Of New-Active-Substances

IntroductionThe orphan designation has been used by the European Medicines Agency to incentivize the development of drugs treating rare diseases with high-unmet medical needs by supporting their development process and economic returns. This study evaluated the impact of the regulatory orphan designation and other drug development-related factors on the rollout times and Health-Technology-Assessment (HTA) recommendations of new active substances (NASs).MethodsA total of 656 HTA appraisals from 6 European countries were collected for NASs that received regulatory approval between 2012 and 2020. Multivariable logistic (positive and positive with restrictions vs. negative HTA recommendation as dependent variable) and linear regression (rollout time as dependent variable) models examined associations with regulatory orphan designation, expedited process, product type (biotechnological vs chemical), and jurisdiction (France, England, Germany, Poland, Scotland and Sweden). Rollout time was defined as months elapsed from regulatory submission to HTA recommendation (mean± standard deviation).ResultsMultivariable logistic regression analysis identified disparities in HTA recommendations between countries. Every month increase in rollout time conferred a 3 percent reduction in the odds of a positive recommendation (p&lt;0.001). Review and product type did not show associations with HTA recommendation. Interestingly, orphan products showed a 99% increase in the odds of obtaining a positive HTA recommendation compared to non-orphan (p-value=0.003). We found 244 appraisals (37%) assessing an orphan product, of which 202 (83%) received a positive HTA recommendation.Multivariable linear regression analysis indicated that orphan products presented a 4.4-month rise in rollout time when compared to non-orphan products (p&lt;0.001). The mean rollout time in months for orphan products were 25±12 in France, 30±15 in England, 21±9.1 in Germany, 37±16 in Poland, 25±12 in Scotland and 27±14 in Sweden.ConclusionsOrphan designated products showed greater odds of receiving a positive HTA recommendation compared with non-orphan. A more detailed review of orphan products could result in their longer rollout time compared with non-orphan counterparts. Considerable differences were found between HTA recommendations and rollout times between jurisdictions.

3-MCPD exposure enhances ovarian fibrosis and reduces oocyte quality in mice

3-monochloro-1,2-propanediol (3-MCPD) is a food contaminant believed to be harmful to human health. Previous studies showed that 3-MCPD exerts toxic effects in multiple tissues, but whether 3-MCPD affects female reproductive function remained unknown. Here, using mouse gastric lavage models, we report that 3-MCPD exposure for four weeks affected body growth, decreased the ovary/body weight ratio, and increased atretic follicle numbers. Expression levels of follicular development-related factors decreased. Further studies found that ovaries from 3-MCPD exposed mice had activated the Transforming Growth Factor-β (TGF-β) signaling pathway and promoted ovarian fibrosis. Increased TNF-α, IL-1 and NF-κB expression also indicated the occurrence of ovarian inflammation. Exposure to 3-MCPD stimulated the caspase pathway and enhanced granulosa cell apoptosis. Consistent with disrupted ovarian homeostasis, 3-MCPD exposure interfered with mitochondrial function, generated more reactive oxygen species, increased ferrous ion and lipid peroxidation levels, and resulted in decreased oocyte development potential. Collectively, these findings indicated that 3-MCPD exposure induced ovarian inflammation and fibrosis, and caused disorders of mitochondrial function and ferrous ion homeostasis in oocytes, which consequently disturbed follicle maturation and reduced oocyte quality.

SLL1-ZH Regulates Spikelets Architecture and Grain Yield in Rice

The spikelet developmental processes that control structure and floral organ identity play critical roles in rice grain yield formation. In this study, we characterized a novel rice mutant, SLL1-ZH, which exhibits a variety of defective agronomic characters, including semi-dwarf, rolling leaf, deformed panicles, and reduced grains production. Morphological analysis also revealed that the SLL1-ZH mutant shows numerous defects of floral organs, such as cracked glumes, hooked and thin lemmas, shrunken but thickened paleas, an indeterminate number of stamens and stigmas, and heterotopic ovaries. Map-based cloning identified a single nucleotide substitution (C to G) in the first exon of LOC_Os09g23200 that is responsible for the SLL1-ZH phenotype. In addition, qPCR analysis showed a significant change in the relative expression of SLL1-ZH in the mutant during inflorescence differentiation and in the different floral organs. Transcription of rice floral organ development-related factors also changed significantly in the mutant. Therefore, our results suggested that SLL1-ZH plays a great role in plant growth, spikelet development, and grain yield in rice.

Water security in Central Asia and Southern Caucasus

The dissolution of the Soviet Union, and the emergence of new States in Central Asia and Southern Caucasus has created new political and security dynamics in these regions. The latter was affected by the Nagorno-Karabakh conflict and the former is confronted by the threat of conflict over shared water resources. More than twenty years later, the protracted conflict in Southern Caucasus over Nagorno-Karabakh remains unresolved and overshadows water-related problems; despite this, the dispute over water is a very important factor in the conflict, and key to overcoming it. In Central Asia, although the threat of conflict over water resources did not materialize, the region remains under the spotlight of water-conflict literature. In the present paper, the complex nature of water security is reviewed through an in-depth analysis of security and development-related factors in their connection to water and vice versa, which leads to the conclusion that policy interventions should be based on a more holistic approach that includes a wider set of issues.

HelPredictor models single-cell transcriptome to predict human embryo lineage allocation.

The in-depth understanding of cellular fate decision of human preimplantation embryos has prompted investigations on how changes in lineage allocation, which is far from trivial and remains a time-consuming task by experimental methods. It is desirable to develop a novel effective bioinformatics strategy to consider transitions of coordinated embryo lineage allocation and stage-specific patterns. There are rapidly growing applications of machine learning models to interpret complex datasets for identifying candidate development-related factors and lineage-determining molecular events. Here we developed the first machine learning platform, HelPredictor, that integrates three feature selection methods, namely, principal components analysis, F-score algorithm and squared coefficient of variation, and four classical machine learning classifiers that different combinations of methods and classifiers have independent outputs by increment feature selection method. With application to single-cell sequencing data of human embryo, HelPredictor not only achieved 94.9% and 90.9% respectively with cross-validation and independent test, but also fast classified different embryonic lineages and their development trajectories using less HelPredictor-predicted factors. The above-mentioned candidate lineage-specific genes were discussed in detail and were clustered for exploring transitions of embryonic heterogeneity. Our tool can fast and efficiently reveal potential lineage-specific and stage-specific biomarkers and provide insights into how advanced computational tools contribute to development research. The source code is available at https://github.com/liameihao/HelPredictor.

Role of the kisspeptin/KISS1 receptor system in the testicular development of mice.

Kisspeptin and its receptor KISS1R have been found to be essential regulators of reproductive function. Previous data have revealed the presence of Kiss1 and Kiss1r mRNAs in the hypothalamus and the testis of humans and rodents. However, the precise location and possible physiological role of the kisspeptin/KISS1R system in the testis remain ambiguous. We first produced an anti-KISS1R immunoglobulin Y antibody for KISS1R identification. To detect the exact sites of KISS1R and kisspeptin expression in the testis, we conducted immunohistochemistry assays on sections of testes. We used real-time polymerase chain reactions to identify Kiss1r in mice and to determine the expression levels of testicular genes. Finally, to verify the upstream regulation on the Kisspeptin/KISS1 receptor system, we treated primary mouse Leydig cells and MA-10 cells with luteinizing hormone (LH) and Br-cAMP, respectively, and examined Kiss1 and Kiss1r mRNA expression. Immunohistochemistry assays revealed that kisspeptin was expressed in Leydig cells and KISS1R was localized in the seminiferous tubules. With real-time polymerase chain reactions, we found Kiss1r mRNA was constitutively expressed in the mouse testis from birth until the postnatal fourth week. Furthermore, mRNA expression of Kiss1 was synchronized with that of Insl3 and Cyp19a. However, the expression of the LH receptor-encoding gene increased 1 week earlier than did Kiss1 expression. This indicated that the kisspeptin/KISS1R system in the testis may be controlled by LH and cAMP signaling pathways. Finally, we confirmed that Kiss1 mRNA expression was increased in both LH-treated primary Leydig cells and Br-cAMP-treated MA-10 cells (p < 0.05). On the other hand, cotreatment of both cell lines with Br-cAMP and a protein kinase A inhibitor RP-cAMP significantly suppressed 50% of Br-cAMP-induced Kiss1 expression (p < 0.05). We discovered that Kiss1 expression in mouse Leydig cells was induced by LH through the cAMP/PKA pathway. Based on the presence of kisspeptin receptors on spermatids, we inferred that kisspeptin- and development-related factors have synergistic effects on spermatogenesis. Nevertheless, more studies are required to elaborate the role of the kisspeptin/KISS1R system in testicular development.

Role of the Kisspeptin/KISS1 Receptor System in the Testicular Development of Mice.

Kisspeptin and its receptor KISS1R have been found to be essential regulators of reproductive function. Previous data have revealed the presence of Kiss1 and Kiss1r mRNAs in the hypothalamus and the testis of humans and rodents. However, the precise location and possible physiological role of the kisspeptin/KISS1R system in the testis remain ambiguous. We first produced an anti-KISS1R immunoglobulin Y antibody for KISS1R identification. To detect the exact sites of KISS1R and kisspeptin expression in the testis, we conducted immunohistochemistry assays on sections of testes. We used real-time polymerase chain reactions (PCR) to identify Kiss1r in mice and to determine the expression levels of testicular genes. Finally, to verify the upstream regulation on the Kisspeptin/KISS1 receptor system, we treated primary mouse Leydig cells and MA-10 cells with luteinizing hormone (LH) and Br-cAMP, respectively and examined Kiss1 and Kiss1r mRNA expression. Immunohistochemistry assays revealed that kisspeptin was expressed in Leydig cells and KISS1R was localized in the seminiferous tubules. With real-time PCR, we found Kiss1r mRNA was constitutively expressed in the mouse testis from birth until the postnatal fourth week. Furthermore, mRNA expression of Kiss1 was synchronized with that of Insl3 and Cyp19a. However, the expression of the LH receptor-encoding gene increased 1 week earlier than did Kiss1 expression. This indicated that the kisspeptin/KISS1R system in the testis may be controlled by LH and cAMP signaling pathways. Finally, we confirmed that Kiss1 mRNA expression was increased in both LH-treated primary Leydig cells and Br-cAMP-treated MA-10 cells (p < 0.05). On the other hand, cotreatment of both cell lines with Br-cAMP and a protein kinase A inhibitor RP-cAMP significantly suppressed 50% of Br-cAMP-induced Kiss1 expression (p < 0.05). We discovered that Kiss1 expression in mouse Leydig cells was induced by LH through the cAMP/PKA pathway. Based on the presence of kisspeptin receptors on spermatids, we inferred that kisspeptin and development-related factors have synergistic effects on spermatogenesis. Nevertheless, more studies are required to elaborate the role of the kisspeptin/KISS1R system in testicular development.

Injury to dopaminergic neurons development via the Lmx1a/Wnt1 autoregulatory loop induced by simazine

Simazine is a kind of persistent organic pollutant that is detected in both ground and water and has several routes of exposure. Here, we explored the mechanisms underlying simazine-related effects on dopaminergic neurons via development-related factors using mouse embryos and embryonic mesencephalic hybrid cell line (MN9D cells). We treated pregnant mice with 50 μg/kg bw, 200 μg/kg bw simazine from the 0.5 day to the 10.5 day of embryonic phase and MN9D cells with 600 μM simazine for 24 h to research the mechanism of dopaminergic neurons acute respond to simazine through preliminary experiments. Protein expressions of LIM homeobox transcription factor 1-alpha (Lmx1a) and LIM homeobox transcription factor 1-beta (Lmx1b) displayed a dose- and time-dependent increase after the exposure to simazine. In the 200 μg/kg bw of embryos and the 24h-600 μM of MN9D cells, protein levels of dopaminergic developmental factors were significantly upregulated, and dopaminergic function was significantly damaged for the abnormal expression of Dyt5b. We demonstrated simazine induced the injury to dopaminergic neurons via the Lmx1a/wingless-related integration site 1 (Wnt1) and Lmx1b pathways. In the transfection experiments, we knocked down Lmx1a and Lmx1b of cells to verify the potential target of simazine-induced injury to dopaminergic neurons, respectively. We detected the protein and mRNA levels of development-related genes of dopaminergic neurons and intracellular dopamine levels in different treatment groups. Based on our experiments’ results, we demonstrated an acute response to 24 h–600 μM simazine treatment, the simazine-induced injury to dopaminergic neuronal which leads to abnormal dopamine levels and dopaminergic impairment is via the activation of the Lmx1a/Wnt1 autoregulatory loop. Lmx1a is a promising target in the search for the mechanisms underlying simazine-induced dopaminergic injury.

Facilitators and barriers to improved cookstove adoption: a community-based cross-sectional study in Northwest Ethiopia

BackgroundAmong the environmental risk factors, household air pollution exposure from traditional cooking practices is one of the biggest killers globally, which mainly impacts developing countries where many families rely on traditional cooking practices. Although improved cookstove adoption is central to tackle this public health issue, the efforts to disseminate cookstove technologies have faced challenges, and the adoption rates are reported to be very low in many developing countries including Ethiopia. Therefore, this study aimed to determine the magnitude and identify potential factors that may act as facilitators or barriers to adoption from users’ point of view.MethodsAs part of the wider stove trial project, a cross-sectional study was conducted among a total of 5830 households under randomly selected clusters. The required data were collected through face-to-face interviews, and a backward stepwise logistic regression analysis technique was applied to evaluate the effect of potential predictor variables on adoption using adjusted odds ratio (AOR) as measures of effect.ResultsThe prevalence of adoption was found to be 12.3% (95% CI 11.5–13.2), and households headed by females (AOR 1.96; 95% CI 1.24–3.10), private house ownership (AOR 4.58; 95% CI 3.89–6.19), separate cooking location (AOR 1.84; 95% CI 1.49–2.78), fuel purchasing (AOR 2.13; 95% CI 1.64–2.76), health benefit (AOR 1.76; 95% CI 1.15–2.70), optimistic social interaction (AOR 1.81; 95% CI 1.46–2.26), traditional suitability (AOR 1.58; 95% CI 1.28–1.95), stove use demonstration experience (AOR 2.47; 95% CI 1.98–3.07), cheap price (AOR 2.48; 95% CI 1.91–3.21), availability (AOR 1.81; 95% CI 1.5–1, 2.17), fuel-saving benefit (AOR 1.63; 95% CI 1.18–2.24), and more durable stove (AOR 1.71; 95% CI 1.30–2.26) of cookstove played a significant role as facilitators to adoption. In addition, lower educational level of head (AOR 0.31; 95% CI 0.23–0.42) and fuel processing requirement (AOR 0.55; 95% CI 0.44–0.70) of cookstove were found to be barriers for adoption.ConclusionsExtremely lower improved cookstove adoption was observed due to household- and setting-related, cookstove technology-related, user knowledge- and perception-related, and financial- and market development-related factors. Therefore, to gain successful adoption, implementers and policymakers should consider those important factors in the implementation of clean cooking solutions to the community.

On the performance of method-level bug prediction: A negative result

Bug prediction is aimed at identifying software artifacts that are more likely to be defective in the future. Most approaches defined so far target the prediction of bugs at class/file level. Nevertheless, past research has provided evidence that this granularity is too coarse-grained for its use in practice. As a consequence, researchers have started proposing defect prediction models targeting a finer granularity (particularly method-level granularity), providing promising evidence that it is possible to operate at this level. Particularly, models mixing product and process metrics provided the best results.We present a study in which we first replicate previous research on method-level bug-prediction, by using different systems and timespans. Afterwards, based on the limitations of existing research, we (1) re-evaluate method-level bug prediction models more realistically and (2) analyze whether alternative features based on textual aspects, code smells, and developer-related factors can be exploited to improve method-level bug prediction abilities. Key results of our study include that (1) the performance of the previously proposed models, tested using the same strategy but on different systems/timespans, is confirmed; but, (2) when evaluated with a more practical strategy, all the models show a dramatic drop in performance, with results close to that of a random classifier. Finally, we find that (3) the contribution of alternative features within such models is limited and unable to improve the prediction capabilities significantly. As a consequence, our replication and negative results indicate that method-level bug prediction is still an open challenge.

Contribution of adipocyte precursors in the phenotypic specificity of intra-articular adipose tissues in knee osteoarthritis patients

BackgroundIntra-articular adipose tissues (IAATs) are involved in osteoarthritis (OA) pathophysiology. We hypothesize that mesenchymal cells residing in IAATs may account for the specific inflammatory and metabolic patterns in OA patients.MethodsAdipocyte precursors (preadipocytes and dedifferentiated fat cells (DFATc)) from IAATs (infrapatellar and suprapatellar fat pads) and autologous subcutaneous adipose tissues (SCATs) were isolated from knee OA patients. The ability of these precursors to differentiate into adipocytes was assessed by oil red O staining after 14 days of culture in adipogenic medium. The gene expression of adipocyte-related transcription factors (C/EBP-α and PPAR-γ) and development-related factors (EN1 and SFRP2) were analyzed. The inflammatory pattern was assessed by RT-qPCR and ELISA (interleukin 6 (IL-6), IL-8, Cox2, and prostaglandin E2 (PGE2)) after a 24-h stimulation by IL-1β (1 ng/mL) and by conditioned medium from OA synovium.ResultsIAAT preadipocytes displayed a significantly higher ability to differentiate into adipocytes and expressed significantly more C/EBP-α mRNA than SCAT preadipocytes. IAAT preadipocytes expressed significantly less EN-1 and SFRP2 mRNA than SCAT preadipocytes. Unstimulated IAAT preadipocytes displayed a less inflammatory pattern (IL-6, IL-8, and Cox2/PGE2) than SCAT preadipocytes. In contrast, the response of IAAT preadipocytes to an inflammatory stimulus (IL-1β and conditioned media of OA synovium) was exacerbated compared to that of SCAT preadipocytes. Similar results were obtained with DFATc.ConclusionIAAT adipocyte precursors from OA patients have a specific phenotype, which may account for the unique phenotype of OA IAATs. The exacerbated response of IAAT preadipocytes to inflammatory stimulation may contribute to OA pathophysiology.

What can open access data from India tell us about road safety and sustainable development?

Transport contributes to socioeconomic development. In the case of rapidly developing and rapidly motorizing societies like India, increased mobility brings an increased burden associated with road crashes and their social and economic consequences. The Sustainable Development Goals explicitly address road safety as necessary to achieving targets related to good health but also as part of the targets leading towards more sustainable cities and communities. We apply Seemingly Unrelated Regression to investigate development-related factors that potentially contribute to differences among urban and rural road crash outcomes using open data sets for 35 states and territories in India. Results indicate that there are relationships among development and crash parameters common to both urban and rural areas. The results indicate different development indicators driving differences in crash outcomes in rural versus urban areas. Study findings are supportive of international efforts to better understand complex relationships among the goals and targets set out in the Sustainable Development Goals and other high-level development-focused declarations such as the New Urban Agenda. It is hoped this paper adds a new perspective on road safety within sustainable development discussions and encourages interdisciplinary research to further explore these relationships across various regions, countries, and contexts.

Economic development and residential water consumption in Chile

AbstractA better understanding of the relative importance of factors related to climate change and to changes associated with economic growth would serve to inform water policy and to focus scarce public resources on anticipated problems arising from distinct sources of changes in water demand. This article investigates the determinants of residential water consumption in Chile, a developing country that has seen noteworthy changes in incomes, household size, poverty rates and levels of urbanization, and which is projected to experience significant climatic but varied changes, depending on the region of the country. Panel data for 1998-2010 at the municipal level is used to analyze the sensitivity of residential water demand to climate and development-related factors. In the case of Chile, the effect on water consumption of these development-related changes is estimated to be several times that of the changes associated with climate projections for 50 to 80 years in the future.

Enhancing change prediction models using developer-related factors

Continuous changes applied during software maintenance risk to deteriorate the structure of a system and are a threat to its maintainability. In this context, predicting the portions of source code where specific maintenance operations should be focused on may be crucial for developers to prevent maintainability issues. Previous work proposed change prediction models relying on product and process metrics as predictors of change-prone source code classes. However, we believe that existing approaches still miss an important piece of information, i.e., developer-related factors that are able to capture the complexity of the development process under different perspectives. In this paper, we firstly investigate three change prediction models that exploit developer-related factors (e.g., number of developers working on a class) as predictors of change-proneness of classes and then we compare them with existing models. Our findings reveal that these factors improve the capabilities of change prediction models. Moreover, we observed interesting complementarities among the prediction models. For this reason, we devised a novel change prediction model exploiting the combination of developer-related factors and product and evolution metrics. The results show that such a combined model is up to 22% more effective than the single models in the identification of change-prone classes.

Di(2‐ethylhexyl) phthalate (DEHP) influences follicular development in mice between the weaning period and maturity by interfering with ovarian development factors and microRNAs

Although studies have shown that di(2-ethylhexyl) phthalate (DEHP) can disrupt ovarian function, few reports have focused on follicular development in mice between the weaning period and maturity, especially with respect to microRNA (miRNA) expression. In this study, 21-day-old ICR mice were administered DEHP at doses of 0, 100, 400, and 1600 mg/(kg d) for 6 weeks by gavage. After DEHP administration, a significant decrease in the expression of follicle development-related factors (including c-kit, kitl, gdf9, and atm) was observed by quantitative real-time PCR (RT-PCR), but no significant difference in the proteins encoded by these genes was observed by Western blot. Bisulfite sequencing suggested that the total methylation percentages of promoter regions of these genes were not notably altered after DEHP exposure. However, RT-PCR revealed a significantly increased expression of ovarian miRNAs (let-7b, miR-17-5p miR-181a, and miR-151), which inhibit follicular granulosa cell proliferation. Overall, this study showed that DEHP administration from weaning to maturity could suppress the mRNA expression of follicular development factors, and this effect was not achieved through changes in the methylation of DNA in CpG islands of development factors. In addition, DEHP was shown to induce miRNAs to inhibit the proliferation of follicular granulosa cells and the anti-apoptosis function of KITL and GDF9 while increasing bax/bcl2 expression to further promote the apoptosis of granulosa cells.