The storage time of Auricularia auricula mycelium was an important factor influencing its growth activity. The rapid detection of storage time had important theoretical significance and practical value for evaluating, cultivating, and scientifically managing Auricularia auricula germplasm resources. Due to the problem of mapping the relationship between the complex near-infrared (NIR) spectral characteristics of Auricularia auricula mycelium and its storage period, it was difficult to characterize using mathematical models accurately. In this paper, a method for detecting the storage period of Auricularia auricula mycelium based on NIR spectral characteristics and deep learning was proposed. First, five different storage periods of Auricularia auricula mycelium were taken as the research object, and the de-trending (DT) algorithm was used to pre-process the spectral data. Then, the Competitive Adaptive Reweighted Sampling (CARS) algorithm was applied to extract the NIR spectral characteristics of the mycelium. The reduction rate of the spectral variables reached 94.64 %. Further, a one-dimensional convolutional neural network (T-CNN) was constructed, and the SGD-Adam algorithm was used to calculate the optimal network parameters. Finally, a method for detecting the storage period of Auricularia auricula mycelium was implemented, achieving a simulation accuracy of 98.33 %. The experimental results revealed that this method outperformed traditional machine learning models such as partial least squares-discriminant analysis (PLS-DA), back propagation (BP), support vector machine (SVM), and k-nearest neighbors (KNN) by an average of 19.56 % in detection accuracy. Additionally, the detection speed was only 0.07 s. This achievement enabled rapid and accurate detection of the storage time of Auricularia auricula mycelium, providing a dependable foundation for identifying the growth activity of Auricularia auricula mycelium. It provided technical support for the scientific management of the edible fungus mycelium, including quality monitoring, evaluation, and screening.