The major challenges to fabricate quantum processors and future nano-solid-state devices are material modification techniques with nanometer resolution and suppression of statistical fluctuations of dopants or qubit carriers. Based on a segmented ion trap with mK laser-cooled ions we have realized a deterministic single-ion source which could operate with a huge range of sympathetically cooled ion species, isotopes or ionic molecules. We have deterministically extracted a predetermined number of ions on demand and have measured a longitudinal velocity uncertainty of 6.3 m/s and a spatial beam divergence of 600 microrad. We show in numerical simulations that if the ions are cooled to the motional ground state (Heisenberg limit) nanometer spatial resolution can be achieved.