Aim and Objective:The aim of this study is to evaluate and compare the effect of one shape, Neolix rotary single-file systems and WaveOne, Reciproc reciprocating single-file systems on pericervical dentin (PCD) using cone-beam computed tomography (CBCT).Materials and Methods:A total of 40 freshly extracted mandibular premolars were collected and divided into two groups, namely, Group A – Rotary: A1 – Neolix and A2 – OneShape and Group B – Reciprocating: B1 – WaveOne and B2 – Reciproc. Preoperative scans of each were taken followed by conventional access cavity preparation and working length determination with 10-k file. Instrumentation of the canal was done according to the respective file system, and postinstrumentation CBCT scans of teeth were obtained. 90 μm thick slices were obtained 4 mm apical and coronal to the cementoenamel junction. The PCD thickness was calculated as the shortest distance from the canal outline to the closest adjacent root surface, which was measured in four surfaces, i.e., facial, lingual, mesial, and distal for all the groups in the two obtained scans.Results:There was no significant difference found between rotary single-file systems and reciprocating single-file systems in their effect on PCD, but in Group B2, there was most significant loss of tooth structure in the mesial, lingual, and distal surface (P < 0.05).Conclusion:Reciproc single-file system removes more PCD as compared to other experimental groups, whereas Neolix single file system had the least effect on PCD.