Determination Of Unsaturated Iron-binding Capacity Research Articles

A fully automated method for determining total serum iron and unsaturated iron-binding capacity using a Technicon AA-II AutoAnalyser system

A colorimetric method is described for determining total serum iron and unsaturated iron binding capacity (UIBC) of serum using Technicon AutoAnalyzer II equipment. The determination of UIBC has been fully automated by means of a simple modification to the Sampler II module, whereby 2 double probes permit the simultaneous aspiration of a sample and of a measured volume of the iron saturating solution. No manifold changes are needed when changing from serum iron to UIBC.

A proposed fluorimetric determination of unsaturated iron-binding capacity.

A completely new method for the analysis of uncombined iron-binding capacity (UIBC) in human serum is proposed. The method is based on the discovery by C. K. Luk that the fluorescence of terbium ion (Tb-3+) is enhanced enormously when it binds to an empty Fe-3+ binding site of transferrin. Tb-3+ cannot displace Fe-3+ from an occupied binding site on transferrin. The analytic procedure is to dilute the serum (in order to lower its ultraviolet absorbance), add a solution of Tb-3+, excite with ultraviolet radiation, and measure the intensity of the resulting green fluorescence. With proper calibration this intensity is a measure of UIBC. Analyses of 65 serum samples previously measured at a hospital laboratory show no obvious difficulty with the method. Its absolute accuracy has not been probed in detail.

Semi-automated micromethod for estimating the unsaturated iron-binding capacity of serum using radioactive iron.

A semi-automated micromethod for the determination of the unsaturated iron-binding capacity (UIBC) of serum using radioactive iron and magnesium carbonate as an adsorbing substance is presented. The method has the advantage of simplicity and speed. The determination of UIBC is carried out on 0.1 ml of serum in duplicate, using autodiluters, at the rate of up to 200 sera per day. The reproducibility of the presented method is comparable to the reproducibility of other methods for the determination of iron-binding capacity. As the method is a simple one it can easily be introduced into laboratories with facilities for radioactive counting.

Method for the determination of unsaturated iron-binding capacity of serum using radioactive iron and magnesium carbonate.

A method for measuring the unsaturated iron-binding capacity of serum using radioactive iron with magnesium carbonate as the adsorbing substance is described. The radioactive iron-magnesium carbonate method, compared with the methods of Caraway (1963), Herbert, Gottlieb, Lau, Fisher, Grevirtz, and Wasserman (1966), and Bothwell, Jacobs, and Kamener (1959), was shorter and simpler and equally reproducible.

A simple radioisotopic micromethod for determination of unsaturated iron-binding capacity of serum

A radioisotopic method for determining serum unsaturated iron-binding capacity is presented. Accuracy and precision comparable to chemical methods are obtained using 0.2 ml of serum. Hemoglobin at concentrations as high as 1% does not interfere and the method can be used in large scale routine application.