Determination Of Triamterene Research Articles

Instantaneous determination of antihypertensive drugs utilizing TLC: Densitometric methods

An accurate, sensitive and time saving thin layer chromatography (TLC) - densitometric method has been developed and validated for determination of two Antihypertensive drug mixtures. Mixture one contains Spironolactone, Furosemide and Anthranilic acid while, mixture two contains Triamterene, Hydrochlorothiazide and chlorothiazide. Mixture 1 uses developing system consist of Hexane, Ethyl acetate and Glacial acetic acid (5ml – 5ml – 0.1ml ) as developing system and 240 nm as scanning wavelength for separation and determination of Spironolactone, Furosemide and Anthranilic acid (metabolite of Furosemide) and mixture 2 uses developing system consist of Ethyl acetate, Methanol, Glacial acetic acid and Ammonium hydroxide (8ml - 1ml - 0.2ml - 0.3 ml) as developing system and 300nm as scanning wave length for separation and determination of Triamterene, Hydrochlorothiazide and Chlorothiazide ( impurity of Hydrochlorothiazide). This method was validated and shown to demonstrate good accuracy and precision according to the International Council for Harmonisation (ICH) guidelines.

Simultaneous determination of Xipamide and Triamterene by zero order, first derivative, derivative ratio and ratio difference spectrophotometric methods.

Validated, rapid and sensitive spectrophotometric techniques were established for simultaneous determination of Xipamide and Triamterene. The first technique based on determination of Triamterene with zero-crossing of Xipamide using zero order method at 367.0 nm. The second technique based on determination of both Xipamide and Triamterene by first derivative method with zero-crossing of Triamterene and Xipamide respectively, at 265.6 and 388.6 nm. Third technique is ratio difference spectrophotometric method depending on obtaining peak amplitude difference at 256.0 and 273.0 nm for Xipamide and 288.0, 302.0 nm for Triamterene. Fourth method is derivative ratio spectrophotometric method depending on obtaining the first derivative of the ratio spectrum with zero-crossing of Xipamide and Triamterene at 365.2 and 308.6 nm; respectively. Linear relationship was obtained upon using concentration range (1.0-10.0 µg/mL) for Xipamide and (1.0-16.0 µg/mL) for Triamterene with LOD less than 0.3 µg/mL for both drugs. The suggested spectrophotometric techniques showed Lower LOD and more sensitivity other than any reported spectrophotometric methods and were applied in pure and dosage form (Epitens®).

Stability-indicating HPLC-DAD method development, validation, and stress degradation studies for triamterene and xipamide in their combined tablet dosage form

A simple stability-indicating high-performance liquid chromatography-diode array detection (HPLC-DAD) method has been developed for the simultaneous determination of triamterene (TRI) and xipamide (XIP) in presence of the degradation products generated in studies of forced decomposition. Drugs were subjected to stress by hydrolysis (acidic, alkaline, and neutral), oxidation, photolysis (254 and 365 nm), and dry and wet heat treatments. Degradation occurs under acidic and alkaline conditions (TRI only), oxidative stress (TRI and XIP), and by photolysis (XIP only), but both drugs were stable under other stress conditions investigated. Separation of the two drugs from all the degradant peaks was achieved within 11 min using C8 column (250 × 4.6 mm, 5 μm) and mobile phase consisting of acetonitrile and 0.05 M phosphate buffer adjusted to pH 4 delivered at a flow rate of 1 mL min−1 using gradient elution system. The drugs were quantified at 220 nm using photodiode array detector, based on peak area. Peak homog...

Spectrofluorimetric Determination of Triamterene in Different Body Fluids and Pharmaceutical Tablets

A spectrofluorimetric method is used to determine 6-phenylpteridine-2,4,27-triamine inpharmaceutical dosage form, Urine and human serum samples. The method is simple, sensitive, and accurateand based on the fluorescence enhancement with increasing concentration of the highly fluorescent triamterenecontaining three amino groups in methanol at pH 6.7. The dynamic range for the determination of triamtereneis 5×10-10 – 9.5×10-7 mol L-1 with detection limit and quantitative detection limit (LOD and LOQ) of 2x10-10 and 6.6x10-10, mol L-1, respectively. The low values of LOD and LOQ indicate the high sensitivity of theproposed method if compared to previous methods for the determination of triamterene. Percentage recoverieswere found to be 99.30 ± 0.65, 98.6 ± 0.75, and 96.60 ± 0.85 for the pharmaceutical tablets, urine, and serumsamples, respectively. The results demonstrated the accuracy as well as the precision of the proposed method.

Determination of triamterene and leucovorin in biological fluids by UV derivative-spectrophotometry and partial least-squares (PLS-1) calibration.

The resolution of binary mixtures of triamterene (TAT) and leucovorin (LV) by application of first-derivative spectrophotometry and by application of Partial Least Squares calibration (PLS-1) was performed. Triamterene is determined in presence of leucovorin directly in the absorption spectra at 358 nm, and leucovorin is determined in the first-derivative spectra at 305.6 nm, zero-crossing of the triamterene. The mean recovery values in urine samples were 102 and 97% for TAT and LV, respectively. Partial Least Squares calibration (PLS-1) multivariate calibration of spectrophotometric data, have been applied to the determination of these compounds in serum and in urine without pretreatment of the samples. The absorption spectra of samples of serum or urine, spiked with triamterene and/or leucovorin, were used to perform the optimization of the calibration matrices by PLS-1 method. Mean recovery values were of 107 and 108% for TAT and LV in serum samples, and 98 and 91% for TAT and LV in urine samples.

Determination of Hydrochlorothiazide and Triamterene in Combined Tablets by Derivative Spectrophotometry

A first-derivative spectrophotometric method for the determination of triamterene, and a fourth-derivative spectrophotometric method for the determination of hydrochlorothiazide in combined tablets is described. Triamterene was analysed using a zero-crossing method by measurement of the first-derivative amplitude of the methanolic solution of the mixture at 241 nm. Hydrochlorothiazide determination was based on the fourth-derivative amplitude at 227 nm with respect to the baseline. The methods were applied to synthetic mixtures and a commercial preparation, and were found to be linear, precise and accurate.