Abstract
Validated, rapid and sensitive spectrophotometric techniques were established for simultaneous determination of Xipamide and Triamterene. The first technique based on determination of Triamterene with zero-crossing of Xipamide using zero order method at 367.0 nm. The second technique based on determination of both Xipamide and Triamterene by first derivative method with zero-crossing of Triamterene and Xipamide respectively, at 265.6 and 388.6 nm. Third technique is ratio difference spectrophotometric method depending on obtaining peak amplitude difference at 256.0 and 273.0 nm for Xipamide and 288.0, 302.0 nm for Triamterene. Fourth method is derivative ratio spectrophotometric method depending on obtaining the first derivative of the ratio spectrum with zero-crossing of Xipamide and Triamterene at 365.2 and 308.6 nm; respectively. Linear relationship was obtained upon using concentration range (1.0-10.0 µg/mL) for Xipamide and (1.0-16.0 µg/mL) for Triamterene with LOD less than 0.3 µg/mL for both drugs. The suggested spectrophotometric techniques showed Lower LOD and more sensitivity other than any reported spectrophotometric methods and were applied in pure and dosage form (Epitens®).
Highlights
Xipamide shown in (Fig. 1a) [1] is a (4chloro-N-(2, 6-dimethyl phenyl)-2-hydroxy-5sulfamoylbenzamide) (C15H15Cl N2O4S) used for the treatment of high blood pressure
Triamterene showed hyperkalemia as its main side effect. To counter this effect it is used in combination with Xipamide to overcome this side effect [4]
The literature review revealed that many methods were reported for determination of Xipamide including Spectrophotometric [5], Spectroflurometric [6], Voltammetric [7] and HPLC methods [8,9,10], and for determination of Triamterene including spectrophotometric [1115], Spectroflurometric [16,17,18], Electrochemical
Summary
Xipamide shown in (Fig. 1a) [1] is a (4chloro-N-(2, 6-dimethyl phenyl)-2-hydroxy-5sulfamoylbenzamide) (C15H15Cl N2O4S) used for the treatment of high blood pressure. Xipamide has an effective action rather than thiazides and decreases the loss of potassium. Triamterene showed in (Fig. 1b) [2, 3] (6-phenylpteridine-2, 4, 7-triamine) (C12H11N7) is a potassium-sparing diuretic preventing hypokalemia in the body and used for the treatment of edema. Triamterene showed hyperkalemia as its main side effect. To counter this effect it is used in combination with Xipamide to overcome this side effect [4]. The literature review revealed that many methods were reported for determination of Xipamide including Spectrophotometric [5], Spectroflurometric [6], Voltammetric [7] and HPLC methods [8,9,10], and for determination of Triamterene including spectrophotometric [1115], Spectroflurometric [16,17,18], Electrochemical [19, 20], and HPLC methods [21, 22]. Few methods were stated for obtaining both Xipamide and Triamterene in a mixture including Spectrophotometric [23, 24] and HPLC methods [25,26,27]
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