Determination Of Sulfite Research Articles

Rapid and reliable determination of sulfide and sulfite in food by a multianalyte electrochemical sensor based on nanoporous gold

AbstractGiven the increasing food safety problems caused by sulfide and sulfite, there is an urgent need to develop a rapid and accurate method for the detection of sulfide and sulfite in food. In this study, a multianalyte electrochemical sensor was successfully fabricated based on glassy carbon electrode (GCE) modified by nanoporous gold (NPG) for the detection of sulfide and sulfite. Owing to the exceptional oxidation activity of NPG towards sulfite and sulfide, the constructed multianalyte NPG/GCE electrochemical sensor achieved highly sensitive detection of sulfide in the concentration range of 50 μM–5 mM with a sensitivity of 21.33 μA mM−1cm−2 and a limit of detection (LOD) as low as 37.53 μM. For the detection of sulfite, the multianalyte NPG/GCE electrochemical sensor exhibited good linearity in the concentration range of 50 μM–5 mM with a sensitivity of 71.76 μA mM−1cm−2 and a LOD of 5.12 μM. In addition, the multianalyte NPG/GCE electrochemical sensor realized the reliable detection of sulfide and sulfite in complex real food samples (such as milk, pickles, red wine, and tap water) with high sensitivity and anti‐interference ability. The multianalyte NPG/GCE electrochemical sensor exhibited many advantageous properties in practical applications, including easy fabrication, sensitivity, rapidity, cost‐efficient, and flexible adaptability, which made it a promising candidate for the rapid and reliable detection of sulfide and sulfite in food.

Quantification of Total Sulfites in Shrimp by BIOFISH 300/3000 SUL Method, Collaborative Study: Final Action 2021.09.

In December 2021, the BIOFISH 300 SUL method for the determination of total sulfites in shrimp was adopted as First Action Official Method of AnalysisSM by the AOAC INTERNATIONAL. A collaborative study was conducted in February 2023 in order to test the reproducibility of the method. The method is based on the use of a benchtop biosensor device that relates the concentration of sulfite to a quantifiable electric current signal. The sensing element, the Biotest, harbors an enzyme that specifically oxidizes sulfite, and the reaction products are electrochemically detected by the device in less than 3 minutes. The sulfite is extracted from the solid using an aqueous based buffer solution, which ensures that all sulfite is present as a free anion. 11 collaborators participated in the study of nine different shrimp samples. Values of RSDr and RSDR obtained from the statistical analysis of valid data ranged from 2.1-8.1% and 7.5-14.3%, respectively, for shrimp samples above the quantification limit of the method, set at 7 mg/kg. These results showed good repeatability and reproducibility of the method, even at concentrations below the legal threshold for sulfite in food, where the reference Optimised Monier-Williams method shows relatively high imprecision.

A modified pararosaniline hydrochloride method for determination of total and bound sulfur dioxide in wine

An improved pararosaniline hydrochloride (PRA) method for rapid and accurate determination of total and bound sulfur dioxide (SO2) in wine was established. The determination conditions for total SO2 were optimized in model wine systems. The pH of the acidified PRA reagent had a significant effect on the color and the maximum absorption wavelength (λmax) of the reaction system, with its optimum pH 0.48 and a λmax at 549 nm. The addition of ethylene diamine tetraacetic acid (EDTA) could effectively shield the interference of coexisting metal ions present in wine, and a novel approach to double background subtraction was confirmed to remove the matrices interference of red wine color. Under the optimum conditions, the linear range for total SO2 in wine was within 1.00–8.00 mg L−1 (R = 0.9975), with the detection and quantification limits (MDL and MQL) found as 0.02 and 0.06 mg L−1, respectively. The recovery of SO2 ranged from 85.73 % to 105.70 % with the relative standard deviations (RSD) of 0.21 % - 7.78 %, suggesting high sensitivity and accuracy of the method. Combining with the optimized Monier-Williams method, the determination of bound SO2 in wine was also achieved. Sulfite determination can be performed in wine using the rapid, accurate and sensitive method without any pretreatment, and the results were comparable with those of the standard methods.

Modification of the electrochemical sensor with NiCo2O4 decorated graphene composite electrode for sulphite determination in food samples

Modification of the electrochemical sensor with NiCo2O4 decorated graphene composite electrode for sulphite determination in food samples

Determination of Sulfites in Dried Fruits by Paper Spray Ionization Tandem Mass Spectrometry.

Sulfite, a widely used food additive, is subject to regulated labeling. The extraction of sulfite as the stable hydroxymethylsulfonate (HMS) form and its quantitative analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been recognized for their good sensitivity, selectivity, and versatility across various food materials. This study aimed to develop a cost-effective and simpler method for sulfite quantitation, while maintaining the superior sensitivity and selectivity of mass spectrometry (MS). To achieve this, we introduced paper spray ionization (PSI), an ambient desorption ionization technique that could achieve the direct measurement of analytes without employing separation. We also employed a novel internal standard (IS) structurally similar to the analyte, replacing the more expensive isotopically labeled IS. Although the PSI-MS/MS method developed in this study exhibited slightly lower analytical performance compared to the conventional LC-MS/MS, it remained effective for sulfite analysis in dried fruits.

CeO2 nanoparticles modified screen-printed carbon electrode: Electrochemical sensing platform for sulfite determination in water samples

In the present work, the CeO2 nanoparticles (CeO2 NPs) were prepared by hydrothermal method and the crystal structure and morphology analysis were studied by using a series of characterizations. Then, the screen-printed carbon electrode (SPCE) modified with CeO2 NPs was employed to develop a simple and novel sensor for sulfite determination in water samples. The CeO2 NPs/SPCE showed significant current response for sulfite oxidation. The use of the developed sensor with differential pulse voltammetry (DPV) method provides a wide linear range for sulfite determination (0.08–870.0 µM) with a low limit of detection (LOD) (0.023 µM). Finally, the results determination of sulfite in water samples showed good recoveries between 96.7% and 102.7% with a relative standard deviation (RSD) less than 3.6%.

Development of a fast and simple electrochemical sensor for trace determination of sulfite using copper(II) triazole complexes

A glassy carbon electrode (GCE) was modified with copper triazole complexes (Cu(L)2) containing three different substituents (OMe, Br, and NO2), and the fabricated electrochemical sensors were used to detect sulfite. The materials were characterized by cyclic voltammetry and scanning electron microscopy (SEM/EDX). The copper(II) 5-methyl-1-(4-nitrophenyl)-1H-1,2,3-triazole-4-carboxylate (Cu(LNO2)2) complex exhibited the best electrochemical performance toward the sulfite electron transfer process regarding onset potential and peak current. Best results were obtained at pH 4, and the following analytical parameters for sulfite detection were determined by amperometry at 0.90 V, as follows: reproducibility (1.9 %), repeatability (1.6 %), limit of detection (0.75 µmol/L), limit of quantification (2.27 µmol/L), dynamic concentration range (10 µmol/L to 5.00 mmol/L, R2 = 0.995), and sensitivity (7.43x10-3 µA µmol−1 L). Precision was evaluated in terms of repeatability, and a relative standard deviation < 15 % was found at three concentration levels. The method́s accuracy was assessed by determining sulfite in commercial wine samples, and acceptable errors were obtained compared to values reported by an external analytical laboratory. The selectivity towards interfering species (Ca2+, Zn2+, Mg2+, NO3−, glucose, sucrose, NaCl, and KCl) and the stability was also evaluated. The practical application of the Cu(LNO2)2/GCE sensor was examined by analyzing the sulfite content in white and red commercial wines.

Determination of free sulfite in food samples with a novel fluorescent probe as gas-diffusion microextraction acceptor phase

Excessive residues of sulfite (SO32−) have negative effects on human health; thus, it is important to monitor SO32− levels in food samples and living cells. In this study, a novel fluorescent probe (E)-4-(2-(2-(7-(diethylamino)-2-oxo-2H-chromen-3-yl) vinyl) −1,1-dimethyl-1H-benzo[e]indol-3-ium-3-yl) butane-1-sulfonate (probe 1) was prepared. This probe was based on a conjugated coumarin–benzindolium system that was detected to exhibit high-response speed and selectivity and sensitivity towards free bisulfite/sulfite (HSO3−/SO32−) anions. Probe 1 was also highly compatible with gas-diffusion microextraction (GDME) because of its good water solubility, reaction rapidness and ratiometric signal response. Extraction and detection tasks could be completed within 30 min under the selected experimental conditions. The developed GDME-FL method showed adequate limits of detection (4.9 μM) and quantification (16.2 μM) for food analysis. The applications of this method for the detection of free HSO3− in product samples were investigated thoroughly. In addition, we successfully applied probe 1 to image lens cleaning paper, onion, Daphnia magna and HeLa cells. Therefore, probe 1 has a promising application potential for measuring the HSO3−/SO32− content of real food samples.

Electrochemical Removal of Dissolved Oxygen for the on-Site Sensing of Free Sulfites in Wines

Sulfites are a commonly used anti-oxydants, food preservatives and anti-browning agents in the food industry (dried fruits and vegetables, pickles and beverages). Sulfites play also an important role in the preservation of wines as they are used at various stages of the winemaking process to protect the wine from oxidation and help the preservation of its flavors, aromas, and color. Sulfites are present in wines as ‘free’ or they can be bound to carbohydrates, lipids, or proteins. Only the free form is active in the wine protection. Winemakers must check, the ‘free’ and total sulfite content of their wine to make sure that the concentration of sulfite is sufficiently high to protect the wine and that the total sulfite concentration is not above the maximum authorized concentration. Samples are thus sent regularly to specialized laboratories for analysis. We report here the development of an electrochemical sensor for the on-site detection of free sulfites in wine.The electrochemical determination of free sulfite can be achieved by either oxidation or reduction. The presence of many interferents (ascorbates, polyphenols) complexifies the determination based on its oxidation, while determination by reduction is simpler as dissolved oxygen is the sole interferent. However, the electrochemical signal of sulfite reduction may be hidden by the reduction of dissolved oxygen. In laboratory conditions, the interference of dissolved oxygen is usually eliminated by displacement through the bubbling of an inert gas (e.g. Ar or N2). However, as sulfite is a gas under acidic conditions, nitrogen bubbling may also remove it from the samples. We report here the principle of the electrochemical removal of dissolved oxygen in the vicinity of a sensing electrode, making on-site detection possible [1]. The local elimination of dissolved oxygen was achieved at a platinum mesh electrode distant from the sensing electrode by 250 µm. The reduction potential was carefully optimized to allow the diffusion of sulfite through the mesh and its detection at the sensor electrode. With such an experimental set-up, the amperometric response for free sulfite in wines was linear in the 7.5 – 200 mg L-1 concentration range and with a limit of detection of 7.5 mg L-1. The samples were analyzed in less than 5 minutes without the need for solution purging with an inert gas, confirming the advantage of local elimination of dissolved oxygen for on-site analysis. A series of 27 wines (14 reds and 13 whites) from various regions of France was analyzed and the results were validated by conventional iodometric titration. Our amperometric sensor demonstrated that there were no interferences other than the one from dissolved oxygen, which is removed. Even ascorbic acid, which is a major interferent for the iodometric method, did not alter the amperometric response [2].The electrochemical system was tested in an independent winery (Dahlenheim, France) to confirm the ability to perform on-site analysis in a representative working environment. The on-site results were validated by both amperometric and iodometric analysis in the lab. The ease of use and versatility of the sensor open possibilities to use this method for the detection of other food additives (e.g. nitrite), which determination is hindered by the presence of dissolved oxygen, in other types of foodstuff.[1] M. Etienne, T.X.H. Le, T. Nasir, G. Herzog, Electrochemical Filter To Remove Oxygen Interference Locally, Rapidly, and Temporarily for Sensing Applications, Anal. Chem. 92 (2020) 7425–7429. https://doi.org/10.1021/acs.analchem.0c00395.[2] J. Gajdár, G. Herzog, M. Etienne, Amperometric Sensor for Selective On-Site Analysis of Free Sulfite in Wines, ACS Sensors. 7 (2022) 2209–2217. https://doi.org/10.1021/acssensors.2c00611.

A practical chromogenic and fluorogenic dual-mode sensing platform for rapid quantification of sulfite in food

Sulfur dioxide (SO2) and its derivatives (HSO3− and SO32−) are widely used in food-processing. Whereas excessive consumption of sulfur dioxide and its derivatives (>0.70 mg·kg−1day−1) severely endangers human health. In this work, we rationally constructed a practical dual-mode probe (dicyanomethylene)-1-methyl-1,4-dihydroquinolin-2-yl)vinyl)-1-methylquinolinium (QMN), which underwent a specific 1, 4-Michael addition with sulfite to afford a noticeable color change from pale yellow to red along with a high-contrast fluorescence turn-on response at 598 nm. QMN has the advantages of rapid response, high signal-to-noise ratio, excellent selectivity, good water-solubility, large Stokes shift and low detection limit (LOD = 31.9 nM). QMN has been successfully used to on-site visually determine sulfite in a diversity of foods with satisfactory recoveries (91.33–111.33 %) and high accuracy (93.74–98.71 %). Furthermore, a portable smartphone-based fluorescence sensing platform was fabricated for on-site determination of sulfite in food with good performance.

Silver nanocluster-based colorimetric/fluorimetric dual-mode sensor for the detection of bromide and sulfite in waters and wastewaters

In this work, the development of a fluorimetric/colorimetric dual-mode nanosensor for the determination of sulfite and fluorimetric determination of bromide involving silver nanoclusters (AgNCs) is reported. SO2 and Br2 were found to significantly modify the optical properties of AgNCs. Particularly, both volatiles weakened the fluorescence of AgNCs, whereas a color change from nearly colorless to yellowish/brown upon exposure of AgNCs to SO2. Accordingly, three smartphone-based optical assays were devised for sulfite and bromide determination, involving in situ volatile generation and enrichment/trapping of the selectively formed volatiles by AgNCs confined in a droplet and exposed to the headspace above the sample. A hydrophobized cellulose substrate acting as drop holder enabled integrating both the enrichment and the subsequent smartphone-based optical detection in a straightforward manner. Smartphone-based digitization of the enriched AgNCs microdrops and subsequent image processing using a smartphone and its integrated App, respectively, were used for quantitative purposes. Under optimal conditions, limits of detection (LODs) of 1.1 µM and 1.5 µM were achieved for the fluorimetric determination of sulfite and bromide, respectively, whereas sulfite was alternatively determined by colorimetric readout, yielding a LOD of 37.0 µM. The repeatability, expressed as relative standard deviation, was found to be in the range of 5.1–5.9 % in all cases (N = 8). The applicability of the method was demonstrated in aqueous samples of increasing complexity, with recoveries in the range 91–109 %. In addition, the responsiveness of AgNCs to SO2 and Br2 rendered them suitable for the monitoring of bromide and sulfite in increasingly relevant advanced reduction processes such as the UV/sulfite system, as demonstrated in this work.

Layered Architectural Fabrication of a Novel Sulfite Nanobiosensor by Encapsulation of Sulfite Oxidase on a Polypyrrole-Multiwalled Carbon Nanotubes Composite Decorated with Platinum Nanoparticles.

The fabrication of a highly selective and ultrasensitive sulfite nanobiosensor based on a layered architectural fabrication aided by the encapsulation of sulfite oxidase (SOx) in Nafion (Naf) matrix on a multiwalled carbon nanotubes-polypyrrole (MWCNTs-PPy) composite decorated with platinum nanoparticles (PtNPs) is described. The MWCNTs are deposited in the inner layer on a Pt electrode during electropolymerization of pyrrole (Py), followed by decoration with a PtNPs layer and subsequent encapsulation of SOx with Naf in the third layer capped with a fourth thin PtNPs layer. Images obtained by field emission scanning electron microscopy (FESEM) reveal that high-density PtNPs are deposited onto the 3D nanostructured inner MWCNTs-PPy layer and the electrochemical behavior is investigated. A large surface area provided by the incorporation of MWCNTs in the composite and decoration with PtNPs enables increased SOx loading, SOx retention, and substantial improvement in sensing performance. The resulting layered PtNPs/SOx-Naf/PtNPs/MWCNTs-PPy nanobiosensor exhibits a fast response time (within 3 s), a linear calibration range of 20nmm - 6m with an excellent sensitivity of 71µA mm-1cm-2 and a detection limit of 5.4nm. The nanobiosensor was effective in discriminating against common interferants and was successfully applied to sulfite determination in real samples.

Biofunctionalisation of Polypyrrole Nanowires Array with Sulfite Oxidase Coupled with the Integration of Platinum Nanoparticles for Ultrasensitive Amperometric Detection of Sulfite

Sulfite determination in foods and alcoholic beverages is a common requirement by food and drug administration organisations in most countries. In this study, the enzyme, sulfite oxidase (SOx), is used to biofunctionalise a platinum-nanoparticle-modified polypyrrole nanowire array (PPyNWA) for the ultrasensitive amperometric detection of sulfite. A dual-step anodisation method was used to prepare the anodic aluminum oxide membrane used as a template for the initial fabrication of the PPyNWA. PtNPs were subsequently deposited on the PPyNWA by potential cycling in a platinum solution. The resulting PPyNWA-PtNP electrode was then biofuntionalised by adsorption of SOx onto the surface. The confirmation of the adsorption of SOx and the presence of PtNPs in the PPyNWA-PtNPs-SOx biosensor was verified by scanning electron microscopy and electron dispersive X-ray spectroscopy. Cyclic voltammetry and amperometric measurements were used to investigate the properties of the nanobiosensor and to optimise its use for sulfite detection. Ultrasensitive detection of sulfite with the PPyNWA-PtNPs-SOx nanobiosensor was accomplished by use of 0.3 M pyrrole, 10 U mL−1 of SOx, adsorption time of 8 h, a polymerisation period of 900 s, and an applied current density of 0.7 mA cm−2. The response time of the nanobiosensor was 2 s, and its excellent analytical performance was substantiated with a sensitivity of 57.33 μA cm−2 mM−1, a limit of detection of 12.35 nM, and a linear response range from 0.12 to 1200 μM. Application of the nanobiosensor to sulfite determination in beer and wine samples was achieved with a recovery efficiency of 97–103%.

Carbon dot and silver nanoparticle–based fluorescent probe for the determination of sulfite and bisulfite via inner-filter effect and competitive redox reactions

A new sensitive fluorescent probe (CDs-AgNP/H2O2) for detecting sulfite and bisulfite (SO32- and HSO3-) based on theinner-filter effect (IFE) between silver nanoparticles (AgNPs) and carbon dots (CDs) was developed. Because of the spectral overlap between the absorption of AgNPs and the excitation of CDs, the fluorescence of CDs can be quenched by AgNPs owing to the IFE. H2O2 weakens the IFE and restores the fluorescence due to the oxidation of AgNPs by H2O2. However, the existence of SO32-/HSO3- can quench the fluorescence again as a result of redox reaction between SO32-/HSO3- and H2O2. The results showed a broad linear range of 20-200 μM with a low limit of detection (3.02 μM) toward SO32-/HSO3-. Thecombination of IFE and redox reaction led to improvement of the sensitivity and selectivity. Theprobe was implemented to measure SO32-/HSO3- in various agricultural products and foods with acceptable results (80.6 to 118.9%recovery).

Application of biohybrid silsesquioxane based material modified with methylene blue for determination of sulphite

Detecting organic and inorganic contaminants is one of the most important environmental concerns today. Consequently, the search for low-cost materials and efficient technologies for solving such a problem has increased, becoming of the utmost importance.Within this context, the present study applied a new nanostructured bioinorganic hybrid material with great potential in the field of nanotechnology: yeast Saccharomyces cerevisiae chemically modified with cubic silsesquioxane. Based on voltammetric measurements with modified graphite paste electrodes with yeast/silsesquioxane and methylene blue (YSB), the value of anodic peak current decreased linearly as the concentration of sulphite increased. The limit of detection obtained was of 0.60 µmol L−1 and sensitivity amperometric (Sa) of 5.8 × 10−4 µA/mol L−1.

Oxygen vacancies rich Co-Mo metal oxide microspheres as efficient oxidase mimetic for colorimetric detection of sulfite

Modulation of surface oxygen vacancies (Ov) by defect engineering is an attractive strategy for designing high performance transition metal oxide nanozymes. In this work, we reported the fabrication of the CoMoO4 oxidase-like nanozyme, i.e. CoMo-300r, by first pyrolyzing the CoMo layered double hydroxide precursor in an air atmosphere at 300 °C and subsequent NaBH4 reduction strategy. Various characterization outcomes confirm the mesoporous structures of both CoMo-300r and no NaBH4 treated CoMo-300, while the specific surface area of CoMo-300 is significantly increased from 103.54 m2·g−1 to 165.24 m2·g−1 after NaBH4 reduction. Also, NaBH4 reduction treatment leads to the formation of Ov rich CoMoO4 and reduced metal ion species on the surface, promoting the redox reaction kinetics. The CoMo-300r prepared at a molar ratio of Co to Mo of 1:2, pyrolysis temperature of 300 °C and 10 mM NaBH4 exhibited the best oxidase-like activity. The oxidase-like activity of CoMo-300r is 2.6-fold times that of no NaBH4 treated optimal CoMo-300. The CoMo-300r can effectively promote TMB oxidation to produce blue color and has the Michaelis-Menten constant of 0.0428 mM, which was lower than most cobalt-based oxide nanozymes and indicated a higher affinity for TMB. In terms of inhibition of SO32− on the CoMo-300r/TMB system, a sensitive SO32− colorimetric sensing platform was established. The linear range and limit of detection for sulfite determination were 0.2–40 μM and 0.09 μM, respectively. The relative standard deviation for the determination of 10 μM sulfite was 1.41% (n = 11). The method was successfully applied to the detection of sulfite in white wine. This work provides a new strategy to fabricate high activity oxidase mimetic for food analysis.

Electrochemical determination of nitrites and sulfites by using waste-derived nanobiochar

Electrochemical determination of nitrites and sulfites by using waste-derived nanobiochar

A fully integrated 3D printed platform for sulfite determination in beverages via gas diffusion membrane extraction and digital video treatment

In this study, we have described a miniaturized, simple, and low-cost device for sulfite determination in beverages by coupling Gas Diffusion Microextraction to paper-based analytical devices. The color change of an acid-base indicator – promoted by the generated gaseous SO2 – impregnated onto the paper surface was monitored in the function of time by video recording using a smartphone. The analytical information was related to the Hue, Saturation, Value (HSV) color space extracted from the video file. The complete analytical platform was built using a 3D printer, allowing the easy fabrication of a low-cost tailored device. Under optimized conditions, a linear relation from 5 to 90 mg L-1 was obtained using 30 µL of the reagent, 1 mL of sample, and 10 min of analysis. The relative standard deviation and the limit of detection were 2.2 % and 1.6 mg L-1, respectively. The method was successfully employed in several beverages, such as juices, soda, and coconut water.

Development of a portable electroanalytical methodology for determination of sulfite in wine using screen-printed carbon electrodes modified with carbon nanotubes

This study describes the development of an electroanalytical methodology applying square wave voltammetry (SWV) to quantify sulfite in wine using a portable system associated with screen-printed carbon electrodes modified with carboxyl functionalized multi-walled carbon nanotubes (SPCE MWCNT-COOH). SWV parameters were optimized using the design of experiments in 0.100 mol L−1 phosphate buffer at pH 4.00 as supporting electrolyte. The sulfite agents showed an oxidation peak around 0.700 V, which corresponds to the species HSO3−, and values of the limit of quantitation and detection of 4.99 × 10−5 and 1.65 × 10−5 mol L−1, respectively, in the linear range of 5.00 × 10−6 to 8.00 × 10−4 mol L−1, and R2 equal to 0.997. The method did not show interference between common molecules found in wine. Furthermore, wine samples were analyzed by the proposed technique and compared with the Brazilian official method (iodometric titration). There was no significant difference between the sulfite concentrations obtained in both procedures. These results show that the proposed methodology is promising for monitoring sulfite agents, employing a low amount of sample per analysis, and the possibility of applying the sulfite determination in loco.

Reagent-less and sub-minute quantification of sulfite in food samples using substrate-integrated hollow waveguide gas sensors coupled to deep-UV LED

The increasing consumption of processed foods demands the usage of chemical preservatives to ensure freshness and extended shelf life. For this purpose, sodium sulfite and its derivatives have been widely used in a variety of food products to inhibit microbial spoilage and for mitigating oxidative decay. However, the excessive consumption of sulfite may cause health problems, thus requiring rapid and accurate analytical methods for the rapid identification of threshold levels. Conventionally, sulfite is volatilized from food samples by acidification followed by trapping of the gaseous SO2 and determination using a suitable analytical technique. Herein, we propose a yet unprecedented reagent-less approach via direct absorbance measurements of gaseous SO2 at 280 nm after sample acidification. The detection system combines a deep-UV LED and a SiC photodiode with a substrate-integrated hollow waveguide (iHWG) gas cell. Absorbance measurements were performed using a log-ratio amplifier circuitry, resulting in noise levels <0.7 mAU. This innovative concept enabled the determination of sulfite in beverages in the range of 25–1000 mg L−1 with suitable linearity (r2 > 0.99) and an analysis time <30 s. The limit of detection (LOD) was calculated at 14.3 mg L−1 (3σ) with an iHWG providing an optical path length of 75 mm. As a proof of concept, this innovative analytical platform was employed for sulfite quantification in concentrated grape juice, coconut water and beer, with suitable accuracy in terms of recovery (83–117%) and favorable comparison with the official Monier-Williams method. Given the inherent modularity and adaptability of the device concept, we anticipate the application of the proposed analytical platform for the in-situ studies addressing sulfite and other volatilized preservatives in a wide variety of food products with tailorable detectability.