Determination Of Sparfloxacin Research Articles

Determination of sparfloxacin and besifloxacin hydrochlorides using gold nanoparticles modified carbon paste electrode in micellar medium

Determination of sparfloxacin and besifloxacin hydrochlorides using gold nanoparticles modified carbon paste electrode in micellar medium AuCPE was used to study the electrochemical behavior of SPAR and BESI using CV and DPV in presence of SDS.

Synthesis and Characterization of Copper-Doped Zinc Selenide Quantum Dots as Fluorescence Probes for Sparfloxacin

Copper-doped zinc selenide quantum dots modified with mercaptopropionic acid were prepared. The fluorescence quenching of the quantum dots was directly proportional to sparfloxacin concentration. A novel method was established to determine sparfloxacin using the copper-doped zinc selenide quantum dots as fluorescent probes. The interaction between the quantum dots and sparfloxacin was investigated by fluorescence and absorption spectroscopies. A linear relationship was obtained between the quenched fluorescence and sparfloxacin concentration from 1 × 10−6 to 1.8 × 10−5 moles per liter in KH2PO4-Na2HPO4 buffer at pH 7.5 using copper-doped zinc selenide quantum dots at 2.9 × 10−6 moles per liter. The limit of detection for sparfloxacin was 2.4 × 10−9 moles per liter. The method was used for the determination of sparfloxacin in tablets and water with satisfactory results.

Highly sensitive fluorescence biosensors for sparfloxacin detection at nanogram level based on electron transfer mechanism of cadmium telluride quantum dots.

A sensitive fluorescence biosensor for determining sparfloxacin (SPF) based on the electron transfer mechanism and the fluorescence quenching effect of SPF to cadmium telluride quantum dots (CdTe QDs) was developed. The mechanism of the interaction between SPF and CdTe QDs was investigated by UV/Vis absorption and fluorescence spectroscopy. The biosensor could be used for the determination of SPF with a high sensitivity. Under optimum conditions, the linear range was from 0.28 to 40 μg SPF ml(-1) with a correlation coefficient of 0.9983, and the detection limit (3δ/k) was 83.7 ng SPF ml(-1). Furthermore, this method has been applied to the determination of SPF in the synthetic environmental water samples and the spiked human serum samples with good results.

Simultaneous determination of sparfloxacin and commonly used H2 receptor antagonists by RP-HPLC: application to in vitro drug interactions

Innovative, simple, economical, and rapid reverse phase HPLC method has been developed and validated for simultaneous determination of sparfloxacin (SPFX) and cimetidine, ranitidine, and famotidine (H2 receptor antagonists) in bulk material, pharmaceutical formulations, and human serum. Chromatographic system was consisting of schemed HPLC system having UV detector set at 232 nm for cimetidine and, 250 nm for famotidine and ranitidine. Purospher STAR C18 (250 × 4.6 mm, 5 μm) column was used, and the mobile phase (methanol, water and ACN 54:41:5 v/v/v pH 2.7 adjusted by phosphoric acid) was delivered at a flow rate of 1.0 ml min−1. The proposed method is specific, accurate (98.02–102.136%), precise (intra-day and inter-day variation 0.501–2.179%) and linear (R 2 > 0.999) with in the desired range 0.3125–25 μg ml−1 concentration. The detection limit and quantification limit were 0.009–0.084 and 0.030–0.255 μg ml−1, respectively. Paired t test was applied to verify the results. The anticipated method is applicable to routine analysis of SPFX and H2 receptor antagonists in pharmaceutical formulations as well as in human serum samples. The proposed method is also applied to study interaction studies of SPFX with H2 receptor antagonists.

Spectrophotometric determination of sparfloxacin in pharmaceutical formulations and urine samples

A simple and sensitive spectrophotometric method has been developed for the determination of sparfloxacin in bulk and pharmaceutical formulations, and in artificial urine. Sparfloxacin was oxidized into a red colored product using ammonium monovanadate in acidic media. The proposed method was successfully applied to the determination of sparfloxacin in different pharmaceutical formulations (tablets) and in a spiked urine sample. The influence of commonly used excipients on the determination of sparfloxacin was studied. Percentage recoveries in the range of 98.0 ± 0.14 % to 100.0 ± 0.20 % were obtained. The observed data have been evaluated statistically which showed high accuracy and precision.

Optimization, Validation and Application of a Capillary Zone Electrophoresis Method for the Assay of Sparfloxacin in Pharmaceutical Formulation

A capillary zone electrophoresis (CZE) method has been developed for the determination of the antibiotic sparfloxacin in tablets. The CZE separation was performed using 75 µm×35 cm fused-silica capillary under the following conditions: 25°C; applied voltage, 12 kV; 25 mM H3PO4-NaOH running buffer (pH 8.5). The detection wavelength was 254 nm. Flumequine was used as internal standard (IS). The method was suitably validated with respect to linearity, limit of detection and quantification, accuracy, precision, specificity, and robustness. The calibration was linear from 10 to 60 µg mL−1 and the limit of detection and quantification were 5.38 and 9.46 µg mL−1, respectively. Recoveries ranging from 95.68%–102.4% were obtained in the determination of sparfloxacin that were spiked to placebos. Excipients in the commercial tablets and degraded products from different stress conditions did not interfere in the assay. The method was successfully applied to the determination of sparfloxacin in pharmaceutical tablets.

Spectrophotometric Determination of Sparfloxacin in Pharmaceutical Preparations by Ternary Complex Formation with Pd(II) and Eosin

A simple, sensitive, and direct spectrophotometric method has been developed for the assay of sparfloxacin in bulk and pharmaceutical preparations. The proposed method is based on the formation of ternary complex between an investigated drug, palladium(II) ion and eosin in the presence of methylcellulose as surfactant and acetate buffer of pH 4.2. Spectrophotometrically, under the optimum conditions, the ternary complex showed absorption maximum at 550 nm, with apparent molar absorptivity of 2.69×104 l mol−1 cm−1, Sandell's sensitivity of 0.01458 µg ml−1 and linearity in the concentration range 1.6–16 µg ml−1. The composition of the ternary complex was studied by Job's method of continuous variation and the result indicated that the molar ratio of SPFX: Pd: eosin is 1∶1∶1. The optimum reaction conditions and other analytical parameters are evaluated. The proposed method was successfully applied for the determination of SPFX in its pharmaceutical product with mean percentage recoveries of 99.71%. The observed data has been subjected to statistical analysis, which revealed high accuracy and precision.

Atomic absorption spectroscopic, conductometric and colorimetric methods for determination of some fluoroquinolone antibacterials using ammonium reineckate

Three accurate, rapid and simple atomic absorption spectrometric (AAS), conductometric and colorimetric methods were developed for the determination of gatifloxacin (GTF), moxifloxacin (MXF) and sparfloxacin (SPF). The proposed methods depend upon the reaction of ammonium reineckate with the studied drugs to form stable precipitate of ion-pair complexes, which was dissolved in acetone. The pink coloured complexes were determined either by AAS or colorimetrically at λ max 525 nm directly using the dissolved complex. Using conductometric titration, the studied drugs could be evaluated in 50% (v/v) acetone. The optimizations of various experimental conditions were described. Optimum concentration ranges for the determination of GTF, MXF and SPF were 5.0–150, 40–440 μg mL −1 and 0.10–1.5 mg mL −1 using atomic absorption (AAS), conductometric and colorimetric methods, respectively. Detection and quantification limits are ranges from 1.5 to 2.3 μg mL −1 using AAS method or 30–45 μg mL −1 using colorimetric method. The proposed procedures have been applied successfully to the analysis of these drugs in pharmaceutical formulations and the results are favourably comparable to the reference methods.

Spectrophotometric determination of sparfloxacin in tablets

Spectrophotometric determination of sparfloxacin in tablets

DETERMINATION OF SPARFLOXACIN IN PLASMA BY DIRECT INJECTION HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH COLUMN SWITCHING

High performance liquid chromatography (HPLC) assay was developed for the determination of sparfloxacin in plasma. The plasma samples were directly introduced onto a HPLC column after filtering through a Molcut II® membrane filter, which removes high molecular proteins. The sparfloxacin in filtrate was separated from interfering substances and concentrated on a pre-column using an ODS stationary phase and then introduced to an analytical column with an ODS stationary phase by column switching. Sparfloxacin and 4′-oxo-enoxacin, as an internal standard, were detected by ultraviolet absorbance at 300 nm. Determination of sparfloxacin was possible over the concentration range 50–2000 ng/mL; the limit of detection was 20 ng/mL. The recovery of sparfloxacin added to plasma was 97.5–100.3% with a relative standard deviation of less than 2.2%. This method is applicable to pharmacokinetic studies.

Stability Indicating Hplc Method for the Determination of Sparfloxacin (Spar)

ABSTRACT A rapid, sensitive and stability indicating method for the determination of sparfloxacin (SPAR) by RP - HPLC has been developed on a Merck RP - Select B (5 μm; 12.5 cm x 4.0 mm) column using a mobile phase of water: acetonitrile: triethylamine (80 : 20 : 0.2 v/v) pH of which was adjusted to 2.6 with orthrophosphoric acid. The flow rate was 1 ml / min. and the detection was carried out at 304 nm using Waters 486 variable wavelength detector. The retention time for SPAR was 7.2 min. Linearity range was from 8 - 1000 ppm. The method showed good precision and accuracy when applied to two brands of tablets containing SPAR. In alkaline media SPAR is stable where as it undergoes degradation in acidic and oxidising conditions generating different degradation products the nature of which is required to be established. The proposed method nicely separates the degraded products from SPAR and hence can be used as stability indicating method for the assay of SPAR.

A Simple High-performance Liquid Chromatographic Assay for Sparfloxacin in Human Plasma

Abstract A simple, rapid and selective high-performance liquid chromatographic (HPLC) method for the determination of sparfloxacin in human plasma has been developed and evaluated. Plasma protein was precipitated with acetonitrile. The drug and the internal standard (furosemide) were eluted from a Nova Pak C18 cartridge column at 50°C with a mobile phase consisting of 5% acetic acid:acetonitrile:methanol (70:15:15% v/v). The column eluent was monitored at 364 nm. Each analysis required no longer than 5 min. Quantification was achieved by the measurement of the peak-area ratio of the analyte to the internal standard and the limit of quantification for sparfloxacin in plasma is 25 ng/ml. The intraday coefficient of variation (CV) ranged from 1.71% to 6.1%, and interday CV from 2.60% to 4.28% at four different concentrations. The absolute recoveries ranged from 98.6% to 104%, and the relative recoveries from 93.6% to 116.4% at four different concentrations. Preliminary stability tests showed that sparfloxaci...