Determination Of Morin Research Articles

Determination of Morin and Quercetin in Fruit Juice Samples using Air-Assisted Liquid-Liquid Microextraction Based on Solidification of Floating Organic Droplet and HPLC-UV

In this study, a rapid and efficient method has been used for the extraction and determination of morin and quercetin in fruit juice samples based on air-assisted liquid-liquid microextraction based on solidification of floating organic droplet and HPLC-UV. The effects of 7 important parameters on the extraction recovery were examined and were optimized by Plackett-Burman design and Central Composite design. According to the Plackett-Burman design results, ionic strength of the sample solutions, the aspiration/dispersion cycles, and the rate and time of centrifuge did not show significant effects on the extraction of morin and quercetin. The optimized conditions of extraction were as follows; the volume of the extraction solvent of 83.6 µL, pH of 4.34 for the sample, and 1-undecanol as extraction solvent. Under these conditions, the linear calibration curve was in the ranges of 1-1000 ng/mL and 0.5-1000 ng/mL for morin and quercetin, respectively, with the determination coefficient values above 0.99. The limit of detection of morin and quercetin was 0.3 and 0.2 ng/mL, respectively. The extraction recoveries for 10 ng/mL of morin and quercetin were 98.9% and 96.5%, respectively; while, relative standard deviations (n = 3) were lower than 3.2%.

Alcohol-based deep eutectic solvent as a carrier of SiO2 @Fe3 O4 for the development of magnetic dispersive micro-solid-phase extraction method: Application for the preconcentration and determination of morin in apple and grape juices, diluted and acidic extract of dried onion and green tea infusion samples.

In this study, a hydrophilic deep eutectic solvent was synthesized as a carrier and disperser of magnetic nanoparticles based on ferrofluid and used to develop the dispersive micro-solid-phase extraction method. Ethylene glycol/tetramethylammonium chloride deep eutectic solvent and SiO2 @Fe3 O4 were used to provide the highly stable ferrofluid with strong sorbing properties without any additional stabilizer, which was employed to extract and determine morin in apple and grape juices, diluted and acidic extract of dried onion, and green tea infusion samples. The dispersibility of SiO2 @Fe3 O4 and prevention of its aggregation in the sample solution were improved using the deep eutectic solvent-based ferrofluid. Also, it facilitated the fast injection of sorbent into the sample solution that led to an increase of the contact surface between the sorbent and analyte, and reduction of the extraction time and consumption of the sorbent. The important experimental parameters influencing the extraction efficiency of morin were examined. Under the optimal conditions, a linear calibration curve was obtained in the range of 3-500µg/L with a determination coefficient of 0.9994. The limits of detection and quantification were of 0.91 and 2.98µg/L, respectively. While an extraction recovery of 97.7% with relative standard deviation of 3.8% (interday) was obtained via three replicated measurements on a 30µg/L of morin standard solution, the enrichment factor was 39.1. Finally, this method was successfully used to extract and preconcentrate morin in various samples, followed with their determination by high-performance liquid chromatography with ultraviolet detection.

Determination of Morin in Maclura cochinchinensis Heartwood by HPLC.

The HPLC-DAD method was developed to determine morin content in Maclura cochinchinensis Corner heartwood extract. The chromatographic separation was performed using a Hypersil BDS C18 column, isocratic solvent system of 0.5% acetic acid in water:acetonitrile (80:20) with 1.0 mL/min flow rate and detected at 355 nm. The standard curve of morin was linear in the range of 7-905 μg/mL. The method was precise with intra-day relative standard deviation (RSD) of lower than 1% and 2.06% for inter-day RSD. The method accuracy represented by percent recover was 99.58%. The highly efficient HPLC system developed from this study could detect morin contents in M. cochinchinensis heartwood samples collected from various locations in Thailand in the range of 0.74-1.57% w/w. This developed method provided a useful standardization procedure of M. cochincihinesis materials for further application in pharmacy and other commercial developments.

Online Coupling of Lab-on-Valve Format to Amperometry Based on Polyvinylpyrrolidone-Doped Carbon Paste Electrode and Its Application to the Analysis of Morin.

The potential capabilities and analytical performance of lab-on-valve (LOV) manifold as a front end to amperometry have been explored for the on-line determination of morin. Meanwhile, the electrochemical behaviors of morin were investigated based on polyvinylpyrrolidone- (PVP-) doped carbon paste electrode (CPE), which found that PVP can significantly improve its oxidation peak current. The excellent amperometric current response was achieved when the potential difference (ΔE) of 0.6 V was implemented in pH 6.5 phosphate buffer solution (PBS) that served as the supporting electrolyte. A well-defined oxidation peak has been obtained in studies using PVP as a modifier of CPE based on the oxidation of morin. The present work introduces the LOV technique as a useful tool for amperometric measurement, documents advantages of using programmable flow, and outlines means for miniaturization of assays on the basis of PVP modified CPE. The proposed method was applied successfully to the determination of morin in real samples, and the spiked recoveries were satisfactory.