Determination Of Iron In Urine Research Articles

Direct determination of iron in urine and serum using graphite furnace atomic absorption spectrometry.

A simple, rapid and low-cost method for the routine determination of iron in urine and serum using graphite furnace atomic absorption spectrometry is described which may provide an alternative to the more widespread automated spectrophotometric methods. The urine and serum samples were simply diluted with water prior to analysis. Matrix modification was found to be redundant. The standard additions technique or the use of matrix matched standards (addition calibration) was found to be unnecessary and, therefore, the calibration was performed using aqueous standards. For serum analysis the degree of dilution could be reduced by using the less sensitive 302.0-nm resonance line, yielding more precise determinations, and for urine analysis, interferences were eliminated by means of a L'vov platform. The interferences that exist in the presence of nitric acid are also discussed. Finally, the presence of background absorption was investigated by means of Zeeman effect atomic absorption.

Rapid determination of iron in urine, in the presence of deferoxamine, by inductively coupled plasma emission spectrometry.

Using a spectrometer with an argon plasma source coupled to a high-frequency magnetic field, we developed a direct method for determining iron in urine of patients being treated with deferoxamine. The detection limit for iron was 75 nmol/L; added iron was satisfactorily recovered; and we observed no interference from deferoxamine at its most commonly used concentrations. Values for between-run and within-run precision (CV) was less than 5%. Correlation of results with those obtained with a colorimetric method involving bathophenanthroline was good (r = 0.96).

Determination of iron and transferrin in normal human urines

A simple and reliable method for the determination of iron in urine has been described. The values for iron and transferrin in normal human urine are given and discussed.

Determination of iron in urine with special reference to the desferrioxamine test.

A simple and rapid method for the determination of ferrioxamine iron in urine, in which the colour reagent is added directly to centrifuged urine after reduction with dithionite, has been critically evaluated and compared with a method of iron determination after wet ashing. The coefficient of variation of both methods was less than 5%. A very high correlation was found between the values obtained with the two methods, but the simple method gave consistently lower values, averaging about 85% of those by the wet ashing method. Iron present in the urine sediment or bound in a soluble form which is not split off by dithionite will not be measured with the simple method. Despite its lower values the simple method gives uniform and reliable results, and is considered suitable for the performance of the desferrioxamine test.

Determination of iron in urine

A method, involving wet ashing followed by reaction with dipyridyl, is described for the determination of iron in urine from patients being treated with chelating agents. Some sources of analytical error and of contamination are discussed. The need to re-investigate analytical procedures before they are applied to different biological materials is emphasised.

Practical Considerations in the Determination of Iron in Urine

Practical Considerations in the Determination of Iron in Urine

Determination of Iron in Urine Using 4,7-Diphenyl 1,10-phenanthroline

Determination of Iron in Urine Using 4,7-Diphenyl 1,10-phenanthroline