Determination Of Hyoscyamine Research Articles

RP-HPLC法测定洋金花中莨菪碱的含量

RP-HPLC法测定洋金花中莨菪碱的含量

A non-aqueous solid phase extraction method for alkaloid enrichment and its application in the determination of hyoscyamine and scopolamine

A non-aqueous solid phase extraction (SPE) method utilizing silica based strong cation exchange (SCX) was developed and optimized for the enrichment of alkaloids. In this method, silica based SCX SPE columns were used for the elimination of non-alkaloid compounds and the preconcentration of alkaloids from the extracts. Mass spectrometry was employed to analyze the alkaloid-enriched fraction, and results showed that the SPE method developed in this study was effective for the removal of non-alkaloids. Then, this pretreatment method was combined with high performance liquid chromatography for the quantification of scopolamine and hyoscyamine from Scopolia tangutica Maxim. The recoveries of scopolamine and (-)-hyoscyamine were 98.51% and 91.12%, respectively. Relative standard deviation values were 1.4% for scopolamine and 1.6% for (-)-hyoscyamine. The linearity was good in the 0.01-0.8 mg mL(-1) range for hyoscyamine and 0.01-0.4 mg mL(-1) range for scopolamine.

Determination of Hyoscyamine and Scopolamine in Four Hyoscyamus Species from Iran

The purpose of the present study was to determine the tropane alkaloid content of four Hyoscyamus species from Iran, i.e. H. pusillus L., H. niger L., H. reticulatus L. and H. kurdicus Bornm. Determination of alkaloids was performed by the HPLC method. Samples were extracted with chloroform-methanol-25% ammonium hydroxide 15:5:1 (V/V/V). HPLC separation was performed on Eurospher C 18 reversed phase column. An isocratic mixture of triethylammonium phosphate buffer (30 Mm, pH 6.2) and acetonitrile (75:25), was used as the eluent. Hyoscyamine and scopolamine were determined by the external standard method at 210 nm. All the four mentioned Hyoscyamus species contained hyoscyamine and scopolamine, but in different amounts. Scopolamine was the predominant tropane alkaloid in H. pusillus, H. niger and H. kurdicus, while, H. reticulatus contained a higher amount of hyoscyamine.

Determination of Hyoscyamine and Scopolamine in Serum and Urine of Humans by Liquid Chromatography with Tandem Mass Spectrometry

A simple method was developed for the analysis of hyoscyamine and scopolamine in human serum and urine using liquid chromatography with tandem mass spectrometry (LC/MS/MS). Hyoscyamine and scopolamine in serum and urine were cleaned up with an Oasis HLB cartridge and a PSA cartridge. The LC separation was carried out on an ODS column, using linear gradient elution with 5 mmol/L IPCC-MS3-methanol as the mobile phase. The mass spectral acquisition was done in the positive ion mode by applying selected reaction monitoring (SRM). The recoveries of hyoscyamine and scopolamine were 86.0-105% from human serum and urine fortified at 0.2 ng/mL and 10 ng/mL. The detection limits of hyoscyamine and scopolamine were 0.02 ng/mL. Four serum and three urine samples of humans poisoned by eating Datura innoxia Mill. were analyzed by this method. Hyoscyamine and scopolamine were detected at the levels of 0.45-3.5 ng/mL in all serum samples and 170-670 ng/mL in all urine samples.

Tropine alkaloids production by hairy root cultures of Datura stramonium and Hyoscyamus niger

Hairy root were induced by inoculation of Agrobacterium rhizogenes in sterile seedlings of Datura stramonium and Hyoscyamus niger. The transformed roots cultures were established and subcultured on a Murashige and Skoog [1] hormone free medium. Several strains of hairy root were obtained from different genotypes of D. stramonium. Determination of hyoscyamine and scopolamine was performed by TLC-densitometry. A high variability of alkaloids production was observed between the different strains. Sucrose concentration as well as mineral composition of the culture medium influence the alkaloidal content of the transformed roots; scopolamine concentration expressed on a dry weight basis reached 0.56% in optimum conditions.

Determination of Hyoscyamine and Scopolamine in Datura innoxia Plants by High Performance Liquid Chromatography

For the determination of the hyoscyamine-scopolamine content in small quantities of fresh plant tissue an HP LC assay was developed; with a sensitivity of about 20 ng alkaloid per run. Analysis of the different organs of Datura innoxia plants are in accord with the concept that hyoscyamine is synthesized in the roots, whereas transformation to scopolamine starts in these organs and proceeds in the aerial parts of the plant.