Determination Of Hydrazine Research Articles

Micelles Mediated Zone Fluidics Method for Hydrazine Determination in Environmental Samples.

An automated flow method for the determination of hydrazine based on the concept of zone-fluidics has been developed. The analyte reacts under flow conditions with p-dimethylamino benzaldehyde (25 mmol L−1) in micellar medium (100 mmol L−1 SDS) to form a stable derivative (460 nm). Micelles mediated catalysis excludes the use of highly acidic environment typical for this kind of reaction. Following careful examination of chemical and instrumental variables, the method allows the determination of hydrazine at the low micromolar level (0.3–10 μmol L−1) in water samples. Real sample analyses (drinking and boiler feed water) resulted in satisfactory results in terms of accuracy with the percent recoveries being in the range of 82–114%.

Хемилюминесцентный метод определения несимметричного диметилгидразина в водных объектах

Для оперативного контроля качества водных сред предложен хемилюминесцентный метод количественного определения несимметричного диметилгидразина (НДМГ) в водных объектах. В основе метода анализа НДМГ лежит определение расхода окислителя на нейтрализацию исследуемого вещества. В качестве окислителя использован перманганат калия, подкисленный концентрированной серной кислотой до рН = 1-2. На первом этапе перманганат калия реагирует с НДМГ, далее количество непрореагировавшего окислителя измеряется в ходе его реакции с люминолом методом хемилюминесценции. По результатам изменения интенсивности излучения света в реакции хемилюминесценции определяется концентрация окислителя в реакционной смеси, что позволяет количественно определить содержание НДМГ в пробе. Концентрацию НДМГ определяют по максимальной интенсивности свечения в системе люминол - подкисленный перманганат калия. Показано, что предлагаемый метод превосходит по точности измерений традиционный фотоколориметрический метод определения НДМГ. При этом хемилюминесцентная реакция позволяет значительно сократить продолжительность снятия показаний и ускорить обработку данных (продолжительность измерений не превышает 2,5 мин), т.е. обеспечить экспресс-анализ НДМГ в растворах в полевых условиях и в мобильных лабораториях. A chemiluminescence mediated procedure for quantitative determination of unsymmetrical dimethyl hydrazine (UDMH) in water bodies is proposed which can be applied for prompt quality control of aqueous media. The UDMH analysis is based on determining an oxidizing agent uptake required for neutralization of the analyzed compound. Potassium permanganate acidified with concentrated sulfuric acid for pH value adjustment up to 1-2 is used as the oxidizing agent. The first step of the procedure involves interaction of potassium permanganate with UDMH followed by chemiluminescence reaction of unreacted oxidant residues with luminol. Measurements of changes in the intensity of light emission in the chemiluminescence reaction are performed for determination of concentration of the oxidizing agent in the reaction mixture, which provides quantitative determination of UDMH content in the sample. The concentration of UDMH is calculated from maximum luminous intensity in the luminol - acidified potassium permanganate system. The proposed method has demonstrated a superior accuracy as compared to the traditional photocolorimetric method for determining UDMH. Moreover, the chemiluminescent reaction allows to reduce significantly the duration of the analytical procedure and accelerates the data processing (the average time of measurement does not exceed 2.5 minutes), i.e. provides express-analysis of UDMH in aqueous solutions, in particular, in the field conditions and mobile laboratories.

QUANTIFICATION OF HYDRAZINE HYDRATE IN IMATINIB MESYLATE AT GENOTOXIC LEVEL BY CHROMATOGRAPHIC METHOD

Hydrazine hydrate has genotoxic effect in nature and so it should be controlled down as Potential Genotoxic Impurity (PGI). Being polar molecule, hydrazine hydrate (N2H4.H2O) has no chromophores present in structure which can follow Lambert beer law, thus it is difficult to analyze. The present work described an accurate and highly sensitive reversed-phase liquid chromatography-UV derivatization method for determination of hydrazine in imatinib mesylate drug substance. The method of quantification was developed by attaching chromophores to hydrazine with derivatization, which helped to increase sensitivity. The derivatization of hydrazine hydrate was performed using 1% methanolic solution of benzaldehyde which acts as derivatizing agent. The derivatized product 1,2-dibenzylidenehydrazine gives maximum absorbance at 300 nm and at this wavelength no interference of solvents and other impurities are noted. Limit of detection for developed method was 0.002 μg/g. The developed method was validated to determine hydrazine content and can be used in quality control for commercial batch release of imatinib mesylate drug substances with a genotoxic specification limit level 1.87 μg/g by HPLC.

A Novel Double Fluorescence‐Suppressed Probe for the Detection of Hydrazine

AbstractHydrazine is an important raw material in medicine and chemical industry. However, N2H4 is very harmful to the environment and human body. Therefore, the detection of hydrazine is particularly important. In this report, a new type of fluorescence probe based on synergistic effect was developed for the detection of N2H4. The fluorescence of the probe was doubly inhibited by the PET (photo‐induced electron transfer) process and the bridled ESIPT (excited‐state intramolecular proton transfer) process. Upon addition of hydrazine, the interrupted PET process and restored ESIPT worked together leading to the fluorescence enhancement. The probe has good specificity for hydrazine. The LOD (limit of detection) was calculated to be 1.45 ppb (0.045 μM) and the linear range was 0.2‐40.0 μM. The contrast experiment showed that the synergistic effect could improve the sensitivity of the probe to hydrazine. In addition, the probe could be used for the quantitative determination of hydrazine in tap water.

A New Fluorescent Turn-on Dual Interaction Position Probe for Determination of Hydrazine.

Hydrazine is an important catalyst and chemical raw material. But it is highly toxic and potentially carcinogenic. We designed a new hydrazine probe based on a synergistic effect by introducing acetate and phthalimide into 2-phenyl-benzimidazole (PBI). Comparative experiments proved that "the dual position interaction" had a "synergistic effect" on fluorescence enhancement. The fluorescence enhancement caused by the probe (15.0 fold) is much larger than the sum of the fluorescence enhancement of the two monomer compounds (2.6 and 1.4 folds, respectively). A theoretical calculation showed an inhibition of the PET process and a recovery of the ICT process led to a fluorescence enhancement. The probe was specific to hydrazine and showed a linear response to it in the concentrations range of 0.2 - 200 μM with a LOD of 0.062 μM (1.99 ppb). Moreover, the probe could detect hydrazine in tap water; the recovery of hydrazine from the tap water was between 98.86 - 103.28%.

Electrochemical preparation of Pt nanoparticles modified nanoporous gold electrode with highly rough surface for efficient determination of hydrazine

A highly sensitive electrochemical sensing platform for hydrazine was developed based on a highly surface-roughened nanoporous gold electrode decorated with Pt nanoparticles (Pt/HNPG). The self-supporting composite electrode is binder-free, which was fabricated via anodization of a commercial AuSn alloy in HCl electrolyte and subsequent electrodeposition of H2PtCl6 precursor in aqueous solution. The morphology and composition of Pt/HNPG electrode were characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectrometer (EDS), high-resolution transmission electron microscopy (HRTEM) and X-ray photoelectron spectroscopy (XPS). The catalytic activity of Pt/HNPG towards electrooxidation of hydrazine was examined. It is found that both Pt nanoparticles loadings and solution pH have strong influence on the electrocatalytic activity. At the potential of –0.1 V (vs. SCE), the fabricated composite electrode showed excellent sensing performance for hydrazine with a wide linear range of 5 μM to 6.105 mM, a low detection limit of 1.03 μM (signal-to-noise ratio (S/N) of 3) and a high sensitivity of 3449.68 μA mM−1 cm−2. Moreover, the sensor exhibited good selectivity, repeatability and stability. It was also successfully applied for the highly sensitive determination of hydrazine in real water samples with satisfactory results.

Facile synthesis of Co3O4/N-doped carbon nanocomposites as efficient electrode material for sensitive determination of hydrazine

Hydrazine non-enzymatic electrochemical determination is regarded as a promising and simple approach which shows great significance in the field of environmental monitoring and medical analysis. By employment of Co3O4/N-doped carbon nanocomposites as electrochemical sensor, the goal of constructing highly sensitive non-enzymatic determination of hydrazine in low concentration level can be achieved. In this work, chitosan is utilized as the nitrogen and carbon source to prepare Co3O4/N-doped carbon nanocomposites via a precipitation method and subsequent thermal treatment. The chemical composition and morphology of Co3O4/N-doped carbon are explored via Raman spectroscopy, XPS, XRD, FESEM and HRTEM. The electrochemical performances of Co3O4/N-doped carbon modified electrode towards hydrazine detection are studied via cyclic voltammetry and amperometry techniques. The fabricated Co3O4/N-doped carbon based sensor exhibited a rather wide linear range from 0.50 to 977.40 μM, an excellent sensitivity of 56.63 μA mM−1 and a very low detection limit of 0.11 μM (S/N = 3). The fabricated sensor also possessed good repeatability, reproducibility, electivity, as well as pretty good stability.

Sensitive electrochemical sensor for hydrazine based on in situ synthesis of Cu3(BTC)2/GO nanocomposite

A new hydrazine electrochemical sensor was fabricated based on in situ synthesis of Cu3(BTC)2/GO nanocomposite. Cu3(BTC)2/GO composite were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier transform infrared spectrometry (FT-IR). Meanwhile, the electrochemical behaviors of hydrazine on the Cu3(BTC)2/GO/GCE were studied by cyclic voltammetry and linear sweep voltammetry. Under the optimized conditions, Cu3(BTC)2/GO/GCE had enhanced electrocatalytic activity toward hydrazine due to synergic catalytic effect between Cu3(BTC)2 and GO. The electrochemical sensor exhibited a wider linear range (1.0 µM to 5000 µM) and a lower detection limit (0.5 μM) as well as good reproducibility and stability. The proposed sensor has also been used for the determination of hydrazine in tap water with satisfactory results.

Fabrication of Zn-MOF@rGO based sensitive nanosensor for the real time monitoring of hydrazine

In the present study, a porous Zn-MOF@rGO (Zinc-Metal organic frameworks and reduced graphene oxide) was prepared via solvothermal technique and utilized for the amperometric determination of hydrazine. Zn-MOF@rGO solution was used to modify gold electrode (AuE) for the superior electrocatalytic oxidation of hydrazine in real water sample. The synthesized material was analyzed using XRD, TEM, FTIR, TGA and BET techniques. TEM studies revealed prismatic shape of Zn-MOF with size ranging between 160 and 180 nm and were well dispersed on the rGO sheets. Also, the incorporation of Zn-MOF into rGO leads to the significant improvement in the performance which can be ascribed to the large surface area, excellent adsorption affinity, chemical stability and high electrical conductivity. This is the first report on the use of Zn-MOF@rGO for the detection of hydrazine. The hydrazine sensor using Zn-MOF@rGO as electrocatalyst displayed a low LOD (8.7 × 10−3 μM), high sensitivity (5.4 × 10−2 μA μM−1 cm−2) and a fast response time (<2s). Moreover, the sensor possessed excellent anti-interference property and reproducibility. Zn-MOF@rGO nanostructures confirmed its great improvement in the electrochemical performance and their realistic applications in sensing devices.

Citrate stabilized gold nanoparticles on graphenic carbon spheres for the selective detection of hydrazine

We have studied the electrocatalytic properties of hydrazine in the alkaline environment by using the stable sensor matrix. Thereby, we have selected the citrate-capped gold nanoparticles (AuNPs) decorated graphenic carbon spheres (GCS). The hydrothermal carbonization method is utilized to prepare the GCS by using reduced graphene oxide (rGO) and glucose. In hydrothermal treatment, the glucose undergoes polycondensation and simultaneously reduced the rGO which results in spherical morphology. These spheres consist of a carbonized nucleus and a hydrophilic surface that facilitates the formation of citrate-capped AuNPs on its surface. This novel composite provides the stable platform to determine the hydrazine in pH 13. Various physicochemical techniques confirm the formation of GCS-AuNPs and also characterized by electrochemical techniques. This GCS-AuNPs modified GCE has showed the good electrochemical performance to the hydrazine oxidation. Also, it revealed the acceptable analytical performance to the determination of hydrazine. The estimated linear concentration range, limit of detection and sensitivity are 0.02 to 530.1 μM, 6 nM and 4.45 μA μM−1 cm−2. Moreover, GCS-AuNPs/GCE shows good reproducibility and good recovery in real sample analysis.

The distinctive sensing performance of cobalt ion in LaBO3 perovskite (B = Fe, Mn, Ni, or Cr) for hydrazine electrooxidation

A single phase rhombohedral LaCoO3 perovskite is prepared by the microwave-assistant citrate method. The as-synthesized perovskite is applied as a facile sensor for hydrazine determination in aqueous 1 M KOH by using electrochemical techniques such as cyclic voltammetry (CV), chronoamperometry (CA), and electrochemical impedance spectroscopy (EIS). The cyclic volyammogram of LaCoO3 modified glassy carbon electrode (GC/LaCoO3) show a well-defined hydrazine oxidation peak of a current, Ip, equals 1.71 mA·cm−2 at an oxidation peak potential, Ep, of +518.8 mV vs. Ag/AgCl. On the other hand, no hydrazine oxidation is observed at modified GC electrodes with other lanthanum-transition metals perovskites, LaBO3 (B is Ni, Mn, Cr, or Fe). EIS data showed a reduction of the charge-transfer resistance value from 220.9 to 3.8 Ω·cm2 by casting LaCoO3 on GC electrode surface, reflecting the powerful electrocatlaytic activity of LaCoO3 towards the hydrazine oxidation. The presented sensor has two linear responses over hydrazine concentration ranges 0.1–15 and 20–60 mM, with sensitivity values of 5.61 mA·cm−2. M−1 and limit of detection (LOD) value of 0.15 nM, respectively. The sensor exhibits a high stability, excellent reproducibility and accepted anti-interferences ability for various inorganic ions and organic compounds.

Colorimetric determination of hydrazine and nitrite using catalytic effect of palladium nanoparticles on the reduction reaction of methylene blue

In this research, hydrazine and nitrite determination was performed based on the reaction of methylene blue with hydrazine in the presence of palladium nanoparticles (PdNPs) as catalyst. The system was monitored spectrophotometrically by measuring the decrease in the absorbance of the methylene blue at 662 nm. Subsequently the inhibitory effect of nitrite on this catalytic reaction was also used for nitrite determination. In fact the depigmentation of methylene blue was postponed by nitrite added to the reaction mixture containing methylene blue, hydrazine and PdNPs. This inhibitory effect enables us to measure nitrite. Several factors influencing the analytical performance of the kinetic method such as concentration of reactants and electrolyte, pH of the sample solution and temperature were studied to achieve the optimum conditions and highest sensitivity. The change in absorption intensity of methylene blue was linearly proportional to the concentration in the range of 6–600 μmol L−1 and 0.3–14.2 μmol L−1 with a limit of detection of 3.85 μmol L−1and 0.21 μmol L−1 for hydrazine and nitrite, respectively. The method was successfully applied to the determination of hydrazine in water samples and nitrite in water, meat and saliva samples.

Ion chromatographic determination of hydrazine in excess ammonia for monitoring graphene oxide reduction reaction

A new ion chromatography method has been developed to study graphene oxide (GO) reduction by monitoring hydrazine concentration in the GO suspension. The method is based on ion chromatographic separation of hydrazine (from excess ammonia) and its selective determination by electrochemical detection. The developed analytical protocol overcame the significant practical challenges of atmospheric hydrazine oxidation and minimised the matrix interference in both separation and detection which result from the excess of ammonium with respect to hydrazine (up to 5.8 × 104 times) in GO reduction experiments. Chromatographic separations were achieved using a high capacity IonPac CS16 cation-exchange column with a 30 mM methanesulfonic acid (MSA) eluent, within an analysis time of less than 20 min. Detection of hydrazine as hydrazinium ion using electrochemical detector was linear between 10 μM and 4 mM, with LOD and LOQ values of 3 μM and 10 μM, respectively. Standard additions confirmed 103 ± 0.8% recovery. The developed method was successfully used to determine the point of complete GO reduction with hydrazine. Reaction curves for GO reduction generated using the method were compared to results from Fourier-transform infrared spectroscopy and Raman spectroscopy to verify the utility of the approach.

A seminaphthorhodafluor-based near-infrared fluorescent probe for hydrazine and its bioimaging in living systems.

Hydrazine (N2H4) has been classified as a potential carcinogen with its high toxicity, which can be readily absorbed through the skin or via breathing directly. Although some fluorescent probes have been developed for imaging of N2H4, very little can be used for imaging of N2H4 in vivo because of its short emission wavelength. In this study, a new colorimetric and near-infrared (NIR) fluorescent probe CF-1 based on a seminaphthorhodafluor dye was successfully designed and used for hydrazine determination. Upon reaction with N2H4, probe CF-1 showed obvious off-on NIR emission spectrum centered at 657 nm, as well as a distinct color change that can be distinguished by the naked eye. The results of fluorescence spectrum experiments indicated that probe CF-1 has high selectivity and low detection limitation (40.6 nM in the solution). Probe CF-1 has low cytotoxicity and was applied to imaging hydrazine in mitochondria of HeLa cells and in zebrafish.

Electrocatalytic oxidation and amperometric determination of hydrazine using a carbon paste electrode modified with β-nickel hydroxide nanoplatelets.

β-Nickel hydroxide nanoplatelets (NPs) were synthesized and used as a modifier on a carbon paste electrode (CPE) for the detection of hydrazine. Synthesis is based on a one-step hydrothermal method using L-arginine that acts as an agent to adjust the pH value and to shape the NPs. They were characterized by field emission scanning electron microscopy, X-ray diffraction and energy-dispersive X-ray spectroscopy. The composition of the modified electrode was optimized by changing the amount of NPs. Best results were achieved using a 10:70:20 weight ratio for nanoparticles, graphite and mineral oil (the binder). The electrochemical properties of the modified CPE were studied by cyclic voltammetry. The surface coverage of the NPs, the electron transfer coefficient and the charge transfer rate constant were calculated. The diffusion coefficient of hydrazine is 1.56 × 10-5cm2s-1. An amperometric method was worked out that has the following figures of merit: (a) A working applied potential of 500mV (vs. Ag/AgCl reference electrode), (b) a linear range that extends from 1 to 1300μmolL-1, (c) a 0.28μmolL-1 detection limit, (d) a relative standard deviation of 1.3% (for n = 3 at a level of 5μmolL-1), and (d) a sensitivity of 1.33μAμmol-1Lcm-2. The performance of the sensor is compared to other nickel-based sensors for hydrazine. The sensor was successfully used to quantify hydrazine in spiked tap water samples, and the recoveries were 97 ± 2.3% (n = 3). Graphical abstract Schematic presentation of hydrazine electrocatalytic oxidation on NP.CPE*. *NP-CPE (β-nickel hydroxide nanoplatelet modified carbon paste electrode).

A sensitive amperometric sensor for hydrazine based on Pt nanoparticles-reduced graphene oxide–multi-walled carbon nanotubes composite

ABSTRACTThe platinum nanoparticles-reduced graphene oxide-multi-walled carbon nanotubes composite (PtNPs-rGO-MWCNTs) has been synthesised by one-step chemical co-reduction strategy in ethylene glycol (EG) system using sodium citrate as reducing agent. The X-ray powder diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), as well as the electrochemical methods have been used for the characterisation of this composite. Benefiting from the large effective surface and good carrier function of rGO-MWCNTs, PtNPs in this nanocomposite have some excellent characteristics such as small particle size, good dispersion, as well as high electrocatalytic activity. Based on this, a new electrochemical sensor for hydrazine has been fabricated using dropping method. Under the optimum conditions, the linear range for the determination of hydrazine by amperometry at 0.20 V (vs. SCE) in phosphate buffer (pH 7.0) is from 2.0 × 10−7 mol L−1 to 2.3 × 10−3 mol L−1. The detection limit and sensitivity is 4.5 × 10−8 mol L−1 (S/N = 3) and 219.7 μA mM−1, respectively. This sensor has some attractive analytical features such as low detection limit, wide linear range, high sensitivity, as well as good stability.

Amperometric sensing of hydrazine by using single gold nanopore electrodes filled with Prussian Blue and coated with polypyrrole and carbon dots.

A nanoprobe for hydrazine sensing is described that is making use of a single gold nanopore electrode (SAuNPEs) that was modified by electro-deposition of Prussian Blue (PB) and then coated with a thin membrane of polypyrrole and carbon dots in order to enhance stability and catalytic activity. Best operated at a low potential of 0.3V vs. Ag/AgCl, the nanosensor display good electrocatalytic activity towards the oxidation of hydrazine, with a linear response in the 0.5-80μM hydrazine concentration range and a 0.18μM detection limit (at S/N = 3). The method was applied to the determination of hydrazine in human urine. Graphical abstract Schematic presentation of the electrocatalytic oxidation of hydrazine using a single gold nanopore electrode that was modified by electro-deposition of Prussian Blue and then coated with a thin membrane of polypyrrole and carbon dots.

Electrochemical study of hydrazine oxidation by leaf-shaped copper oxide loaded on highly ordered mesoporous carbon composite

Hydrazine is a possible human carcinogen because of its hyper toxicity. Therefore, the determination of hydrazine is particularly important for environmental protection and public security. Electrochemical method for the detection of hydrazine has drawn great interests because of its high sensitivity, fast response time, simple operation, and low cost. In this work, a leaf-like copper oxide (CuO) anchored onto wormlike ordered mesoporous carbon (OMC) composite was prepared for the construction of an electrochemical sensing platform for hydrazine. Because of the synergetic catalytic effect and unique structural properties, the obtained nanosized CuO incorporated in OMC exhibited good electrocatalytic performance for the oxidation of hydrazine with a catalytic rate constant (kcat) of 1.28 × 105 M−1s−1. Moreover, the content ratio of CuO: OMC was optimized to improve the electrocatalytic performance. The CuO/OMC hybrids can act as a sensitive and effective sensing platform for the determination of hydrazine, exhibiting wide linear range, high sensitivity, low limit of detection, and good stability. This comprehensive and systematic study may provide great opportunities for the design and construction of electrochemical sensors for environmental monitoring.

Sensitive and Rapid Flow Injection Amperometric Hydrazine Sensor using an Electrodeposited Gold Nanoparticle Graphite Pencil Electrode

A gold (Au) nanoparticle-modified graphite pencil electrode was prepared by an electrodeposition procedure for the sensitive and rapid flow injection amperometric determination of hydrazine (N2H4). The electrodeposited Au nanoparticles on the pretreated graphite pencil electrode surface were characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction spectroscopy, and electrochemical impedance spectroscopy. Cyclic voltammograms showed that the Au nanoparticle-modified pretreated graphite pencil electrode exhibits excellent electrocatalytic activity toward oxidation of hydrazine because the highly irreversibly and broadly observed oxidation peak at +600 mV at the pretreated graphite pencil electrode shifted to −167 mV at the Au nanoparticle pretreated graphite pencil electrode; in addition, a significant enhancement in the oxidation peak current was obtained. Thus, the flow-injection (FI) amperometric hydrazine sensor was constructed based on its electrocatalytic oxidation at the Au nanoparticle-modified pretreated graphite pencil electrode. The Au nanoparticle-modified pretreated graphite pencil electrode exhibits a linear calibration curve between the flow injection amperometric current and hydrazine concentration within the concentration range from 0.01 to 100 µM with a detection limit of 0.002 µM. The flow injection amperometric sensor has been successfully used for the determination of N2H4 in water samples with good accuracy and precision.

Hydrazine. Determination in workplace air with high performance liquid chromatography – spectrophotometric technique

Anhydrous hydrazine in room temperature is colorless fuming oily liquid with ammonia-like odor. It is used in various industries for electrolytic plating of metals on glass and plastics, as a chemical intermediate for the synthesis of pesticides, insecticides, medicines and days. It is used also as water treatment agent in energy industry (corrosion inhibitor), rocket propellant and as explosives material. Long term exposure to hydrazine may cause to skin irritation and allergic reactions. Diluted aqueous solutions of hydrazine may be irritating for skin, eye and respiratory tract. Epidemiologic studies shows that chronic exposure to hydrazine may cause cancer. In European Union hydrazine is classified as a carcinogenic substance (cat. 1B). Experts from International Agency for Research on Cancer (IARC) have classified hydrazine as a compound probably carcinogenic to humans (Group 2A). Due to decreasing of MAC value for hydrazine in Poland it was necessary to develop and validate a sensitive method for determining hydrazine concentrations in the workplace air in the range from 1/10 to 2 MAC values, in accordance with the requirements of Standard No. PN-EN 482. The study was performed using a liquid chromatograph with spectrophotometric detection. All chromatographic analysis were performed with Discovery LC-18 150 × 2,1 mm analytical column, which was eluted with mixture of acetonitrile and water (6:4 v/v). The method is based on the collection of hydrazine on glass fiber filter impregnated with sulfuric acid, extraction with mixture of sodium dihydrogen phosphate and EDTA, derivatization of extracted compound with benzaldehyde and chromatographic determination of resulted solution with HPLC technique. The method is linear (r = 0.9989) within the investigated working range 0.15–3.5 μg/ml (0.00125–0.029 mg/m3 for a 240-L air sample). Calculated limit of detection (LOD) and limit of quantification (LOQ) were 0.0007 μg/ml and 0.0023 μg/ml, respectively. The average extraction efficiency of hydrazine from filters was 97% and samples stored in refrigerator are stable for 14 days. The analytical method described in this paper enables determination of hydrazine in workplace air. The method is precise, accurate and it meets the criteria for procedures for measuring chemical agents listed in Standard No. PN-EN 482. The method can be used for assessing occupational exposure to hydrazine and associated risk to workers’ health. The developed method of determining hydrazine has been recorded as an analytical procedure (see appendix). This article discusses the problems of occupational safety and health, which are covered by health sciences and environmental engineering.