Here in, we investigate an alternative approach to synthesis of the copper/zeolitic imidazolate framework-8 integrated by boron nitride, Cu/ZIF-8/BN, nanocatalyst on the glassy carbon electrode (Cu/ZIF-8/BN/GCE) by the electrochemical and chemical methods. The structural properties, surface morphology and electrochemical behavior of the Cu/ZIF-8/BN/GCE were studied by X-ray diffraction, scanning electron microscopy, energy dispersive X-ray spectroscopy, Brunauer-Emmett-Teller analyzer and electrochemical impedance spectroscopy techniques. In other electrochemical studies, Cu/ZIF-8/BN/GCE shows enhanced electrocatalytic activity toward oxidation of clopidogrel (CPG) in phosphate buffer solution [catalytic rate constant (kcat = 15.6 × 105 M−1s−1)]. So that, the proposed sensor; Cu/ZIF-8/BN/GCE demonstrates high electroanalytical performances in determination of CPG through the cyclic voltammetry (CV) and amperometry methods under optimum conditions. In amperometric determination, the present sensor offered two linear ranges for CPG from 5 to 39 μM and from 39 to 107 μM, with a detection limit of 3.5 μM. Preliminary real analytical applicability of the developed sensor was investigated in blood serum and pharmaceuticals samples. Our results show that the modified electrode; Cu/ZIF-8/BN/GCE, can serve as an alternative quantitative tool for the rapid detection of CPG in clinical analysis.