Determination Of Chloramphenicol In Milk Research Articles

Automated high-performance liquid chromatographic determination of chloramphenicol in milk and swine muscle tissue using on-line immunoaffinity sample clean-up

An on-line high-performance liquid immunoaffinity chromatographic (HPLIAC) system for the direct determination of chloramphenicol in milk and swine muscle tissue is described. The system consisted of a dual-column system in which an HPLIAC column was directly coupled to an RP-8 high-performance liquid chromatographic column. Skimmed and deproteinated milk or aqueous muscle tissue extract was directly injected into the HPLIAC column. After a washing step with phosphate-buffered saline, chloramphenicol was desorbed by a glycine—NaCl buffer (pH 2.8) and directly concentrated on the RP-8 column. Next, chromatography was carried out using acetonitrile—sodium acetate buffer as the mobile phase. Chloramphenicol was detected at 280 nm. Mean recoveries from spiked raw milk were 70 ± 2% (1–50 μg/kg) and from spiked swine muscle tissue 64 ± 2% (10–50 μg/kg). The calibration curves were linear in the range 1–200 μg/kg spiking levels. Limits of determination were 1 μg/kg for milk and 10 μg/kg for muscle tissue.

Method for the isolation and liquid chromatographic determination of chloramphenicol in milk

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMethod for the isolation and liquid chromatographic determination of chloramphenicol in milkAustin R. Long, Lily C. Hsieh, Ada C. Bello, Marsha S. Malbrough, Charles R. Short, and Steven A. BarkerCite this: J. Agric. Food Chem. 1990, 38, 2, 427–429Publication Date (Print):February 1, 1990Publication History Published online1 May 2002Published inissue 1 February 1990https://doi.org/10.1021/jf00092a019RIGHTS & PERMISSIONSArticle Views155Altmetric-Citations24LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (440 KB) Get e-Alerts Get e-Alerts

Gaschromatographische Bestimmung von Chloramphenicol‐Rückständen in tierischem Material

On the basis of the relevant literature and of their own experimental work, the authors describe extraction, cleaning and derivatization procedures for the determination of chloramphenicol in milk, muscular substance, urine and bile. Chloramphenicol was determined as bis-(trimethylsilyl)-ether using a gas chromatograph fitted with an electron capture detector. To evaluate the analytical techniques, the authors estimated the sensitivity, accuracy and precision for residue amounts of 0.1 and 0.05 mg of chloramphenicol/kg of material under investigation. The limit of detection was 0.02 mg/kg for meat, and 0.01 mg/kg for milk, urine and bile.

Polarographic determination of chloramphenicol

The electroreduction of chloramphenicol has been studied by polarography, cyclic voltammetry, chronopotentiometry and coulometry. The experimental results lead to the conclusion that the drug undergoes a slow 2-electron reduction of the nitro group which is followed by a fast 2-electron reduction to hydroxylamine. At more negative potentials the hydroxylamine group is further reduced to the amine. In acetate buffer pH 4.7 with 0.003 % decylamine present, the drug produces a very well-defined, diffusion-controlled 4-electron polarographic wave. The current is proportional to the concentration and permits the drug to be determined by polarography in the concentration range 0.3–600 μg ml -1. A rapid polarographic method for the determination of chloramphenicol in milk is proposed.