Determination Of Calcium Levels Research Articles

Intra Nasal Use of Ethylene Diamine Tetra Acetic Acid for Improving Olfactory Dysfunction Post COVID-19.

COVID-19 has been associated with olfactory dysfunction in many infected patients. The rise of calcium levels in the nasal secretions plays an essential role in the olfaction process with a desensitization effect on the olfactory receptor neurons and a negative impact on the olfaction transmission. Ethylene diamine tetra acetic acid (EDTA) is a chelating agent that can bind free calcium in the nasal secretions, thereby reducing the adverse effects of calcium on olfactory function. The objective of this work is to demonstrate the effect of intranasal EDTA on improving olfactory dysfunction following COVID-19. Fifty patients with a history of COVID-19 and olfactory dysfunction that persisted for more than 6 months were enrolled in the current prospective randomized clinical trial. Participants were randomized into 2 equal groups. Twenty-five patients were treated with olfactory training only, while the remaining 25 patients received treatment with olfactory training and a topical nasal spray of ethylene diamine tetra acetic acid. The olfactory function was assessed before treatment and 3 months later using the Sniffin' Sticks test. Additionally, the determination of calcium level in the nasal secretions was performed using an ion-selective electrode before treatment and 3 months later. Eighty-eight percent of the patients treated with olfactory training in addition to EDTA exhibited clinical improvement, while 60% showed improvement in patients treated with olfactory training only. Furthermore, a significant decrease in the measured calcium level in the nasal secretions was demonstrated after the use of ethylene diamine tetra compared to patients treated with olfactory training only. Ethylene diamine tetra acetic acid may be associated with an improvement of the olfactory function post-COVID-19.

Penetapan Kadar Kalsium Pada Daun Krokot (Portulaca Oleracea L.) Secara Kompleksometri

Research on the determination of calcium levels in purslane (Portulaca oleracea L.) leaves has been carried out using the indirect complexometric titration method. Fresh purslane leaves were extracted using 3N HC1, dissolved calcium was added to an excess of 0.05 M NazEDTA solution and the excess Na EDTA was titrated with 0.05 M MgSO4 solution using an EBT indicator. Obtained calcium levels in fresh purslane leaves of 0.1503% w/w.

Determination of Calcium Levels with XRF and Literature Review of its Bioavailability in Vitro of Dahlia Tubers Syrop (Dahlia Pinnata Cav.)

Calcium bioavailability (Ca) states the ratio of available calcium to the amount of calcium consumed during metabolic processes. The bioavailability of Ca is calculated by the difference between the amount of Ca in the consumed food product and the excreted feces. In this research, calcium can be obtained from dahlia tuber syrup (Dahlia pinnata Cav.). The purpose of this study was to discuss the determination of Ca levels and a literature review of the Ca bioavailability of dahlia tuber syrup (Dahlia pinnata Cav.) in vitro. The content of Ca in dahlia tubers was determined experimentally using an XRF (X-Ray Fluorescence) instrument. The bioavailability of Ca was determined by predicting the ratio of inulin and Ca content. The results showed that the Ca content of dahlia tuber syrup was 197,57 ppm. The bioavailability of Ca in dahlia tuber syrup in vitro was predicted from a relatively high ratio of inulin content of around 65-86% and FOS levels from hydrolysis of 5% dahlia tuber inulin about 15,41-29,38% or an average of 22,395%, on Ca levels is 81,46-85,33%. The results of the study on the bioavailability of Ca in dahlia tuber syrup (Dahlia pinnata Cav.) have a fairly high level. The high bioavailability of Ca can increase calcium absorption in the body and have health effects, among others, for heart and bone health.

Analysis of calcium levels in beef bones from kaledo waste

Kaledo is one of the special culinary from Central Sulawesi which containing beef bones. The large number of restaurants that provide kaledo in their menus resulted in the amount of beef bone waste that cannot be consumed. This waste can cause environmental problems. However, the bones have the number of minerals, one of them is calcium (Ca). Ca is a mineral that is needed by the body in maintaining body functions. This study aimed to determine the calcium levels in beef bones from kaledo waste which was taken randomly from the kaledo restaurant in Palu. Determination of calcium levels in beef bones from kaledo waste used atomic absorption spectrophotometer. The results of the analysis showed that the water content was 4.5950%, and the ash content was 61.7424%. The analysis of calcium levels measured at a wavelength of 422.7 nm was 19,6800 mg/L. The results of this study are expected to be a source of information about the levels of calcium contained in cow bones from kaledo waste, and it can be used further as a substance in the utilization of beef bones from kaledo waste as a food that can meet the body’s need for calcium intake in preventing stunting.

Determination of Calcium Level in Milk Powder and Its Relation with Nutrition Rickets by Use FE- SEM With EDS

Determination of Calcium Level in Milk Powder and Its Relation with Nutrition Rickets by Use FE- SEM With EDS

Clinical observation of generalized sarcoidosis with damage of bones

Sarcoidosis is a multisystem disease of unknown etiology, at the basis of which there is an inflammatory granulation epithelioid process without a pronounced exudative reaction and without caseous necrosis. This disease has various manifestations. The aim of the study was to investigate the clinical case of generalized sarcoidosis with bone lesion in a patient M., born in 1987. There were performed X-ray examinations, computed tomography (CT), fibrobronchoscopy, determination of calcium levels, angiotensin converting enzyme (ACE), soluble IL-2 receptor in the blood. Dynamic observation was performed for 10 months. The patient started methylprednisolone therapy at a dose of 20 mg/day. Significant positive effect of this treatment was noted. Thus, when patients complain of the bone and joint system problems, examination plan should include radiological examination of thoracic organs, which will allow us to avoid late diagnosis of sarcoidosis or other multisystem diseases that may have similar clinical manifestations.

Determination of Calcium Level (Ca) and Iron (Fe) on Red and Green Spinaches at Parigi Moutong

This research aims to determine calcium level (Ca) and iron (Fe) on red and green spinaches at Parigi Moutong. The method used in this research is spectrodirect method. The research result shows that the calcium level (Ca) and iron (Fe) on green spinach was 0.97 mg/kg and red spinach was 58.33 mg/kg at Pelawa village, while the calcium level (Ca) and iron (Fe) on green spinach was 0.62 mg/kg and red spinach was 50.39 mg/kg. From the research results obtained, it can be seen that the level of calcium and iron on red and green spinach in where the red spinach levels higher than the green spinach levels.

Synthesis and Determination Level of Calcium-Phosphate Hydroxyapatite (HAp) from Chicken Eggshell

<p>The purpose of this study was to synthesize bio-ceramics hydroxyapatite (HAp) from local chicken eggshell and broiler chicken eggshell. The synthesis was done by using precipitation method with sintering temperature 1000ºC. In addition, to determine the levels of calcium-phosphate contained in HAp bioceramics AAS and Spectrophotometer UV-Vis were used. The results show that of 10 grams of each eggshell powder can produce 8.8-9.8 g of HAp powder. Determination of calcium level in HAp by AAS was about ± 71.23% from local chicken eggshell and ± 78.62% from broiler chicken eggshell. Whereas determination of phosphate level in HAp using Spectrophotometer UV-Vis was about ± 67.22% from local chicken and ± 73.75% from broiler chicken eggshell.</p>

Magnevist ® (gadopentetate dimeglumine) does not interfere with the determination of calcium levels in plasma or serum

Evaluation of the possible influence of Magnevist® (Gd- DTPA, gadopentetate dimeglumine, Schering) on the in vitro measurement of plasma calcium level with the commercially available colorimetric and photometric techniques.

Effects of epinephrine on the myocardial performance and haemodynamics of the isolated rat heart during moderate hypothermia — importance of calcium homeostasis

Controversies exist concerning myocardial performance in hypothermia. We have studied the effects of epinephrine on myocardial function at various calcium concentrations in moderate hypothermia (28°C) and normothermia (37°C) using an isolated antegrade perfused rat heart. The maximum pressure velocity (d P/d t max) developed was significantly higher in normothermia compared with hypothermia and was improved by the addition of calcium in both circumstances. Peak negative pressure velocity (d P/d t min) was significantly higher at 37°C compared with 28°C, and was increased by the addition of calcium in normothermia; in contrast to hypothermia, in which no change of d P/d t min was observed. A reduction in cardiac output and stroke volume by hypercalcaemia was observed in hypothermia. The addition of epinephrine improved d P/d t max and d P/d t min, but had a depressive effect on stroke volume and cardiac output at normal and elevated calcium concentrations. Myocardial efficiency was significantly higher during hypothermia compared with normothermia, but was impaired by epinephrine during hypothermia. The variable or even paradoxical effects of epinephrine suggest the need for careful haemodynamic monitoring and determination of calcium levels in hypothermia. The impairment of myocardial performance may be explained by impaired diastolic relaxation and calcium overload.

Autofluorescence as a confound in the determination of calcium levels in hippocampal slices using fura-2AM dye

Recent publications have reported calcium level determinations in slices of brain using imaging techniques and the dye fura-2AM. In general these studies ignore or deal only perfunctorily with the problem of autofluorescence in slices. This confound, which is a result of the pyridine nucleotides that are normally present in tissue, has been previously reported to interfere with Ca 2+ measurements in slices. Because these pyridine compounds are involved in cell metabolism, the fluorescence intensity is labile over time following experimental manipulations. We were studying Ca 2+ levels in hippocampal slices using standard imaging techniques. We found significant and variable autofluorescence at the wavelengths used for calcium determination which interfered with data interpretation in fura-treated slices. The intensity of this autofluorescence is an additive effect and is not large enough to be observed when imaging monolayers. In this paper we present a method for conducting experiments and analyzing data that decreases interference from autofluorescence. Experiments were carried out on both slices bulk loaded with fura-2AM and slices loaded with control buffer. A point to point subtraction of the control slice values gave representative calcium fluorescence values. Hippocampal slices were challenged with sodium cyanide or kainic acid. The metabolic response, seen in the fura-free slices, and the calcium response varied within and between these two treatments. Regional differences in the hippocampal sub fields were also demonstrated in response to the two treatments. These corresponded to known regional vulnerabilities to cyanide and kainate. We conclude that autofluorescence in slices need be considered when determining calcium concentrations using fura-2AM.