Effects of epinephrine on the myocardial performance and haemodynamics of the isolated rat heart during moderate hypothermia — importance of calcium homeostasis

Abstract

Controversies exist concerning myocardial performance in hypothermia. We have studied the effects of epinephrine on myocardial function at various calcium concentrations in moderate hypothermia (28°C) and normothermia (37°C) using an isolated antegrade perfused rat heart. The maximum pressure velocity (d P/d t max) developed was significantly higher in normothermia compared with hypothermia and was improved by the addition of calcium in both circumstances. Peak negative pressure velocity (d P/d t min) was significantly higher at 37°C compared with 28°C, and was increased by the addition of calcium in normothermia; in contrast to hypothermia, in which no change of d P/d t min was observed. A reduction in cardiac output and stroke volume by hypercalcaemia was observed in hypothermia. The addition of epinephrine improved d P/d t max and d P/d t min, but had a depressive effect on stroke volume and cardiac output at normal and elevated calcium concentrations. Myocardial efficiency was significantly higher during hypothermia compared with normothermia, but was impaired by epinephrine during hypothermia. The variable or even paradoxical effects of epinephrine suggest the need for careful haemodynamic monitoring and determination of calcium levels in hypothermia. The impairment of myocardial performance may be explained by impaired diastolic relaxation and calcium overload.