The daily and annual temperature differences in mid and high latitudes areas vary greatly, which leads to thermal fatigue deterioration of concrete. The deterioration of concrete properties can also be generated by the cyclic change of humidity in concrete. In the experiment, the thermal fatigue tests of concrete with two strength grades were carried out at the temperature difference of 20 °C, 30 °C, and 40 °C (the initial temperature is 20 °C) under the condition of constant ambient humidity. The macroscopic properties change of concrete such as compressive strength and splitting tensile strength were measured. The microstructure changes of concrete were determined by ultrasonic nondestructive testing technique and mercury intrusion test. The results indicated that thermal fatigue has obvious deterioration effect on concrete. With the increase of temperature difference and cycle number, the strength of concrete decreased dramatically and the decrease of C40 concrete was greater than that of C25 concrete. The splitting tensile strength was more sensitive to thermal fatigue than compressive strength. The ultrasonic wave velocity gradually decreased and the damage factor increased, indicating the internal crack defects of concrete increased. At the same temperature difference, the porosity, total pore volume, average pore diameter, medium pore diameter, and the most probable pore diameter of concrete increased gradually with the increase of the cycle number, while the total pore surface area decreased. The pore structure showed the characteristics of coarsening and the trend of deterioration. The porosity of C40 concrete was less than that of C25 concrete, but the relative change value of porosity was larger, which reveals the internal reason for the strength damage of concrete under thermal fatigue at the microscopic level.
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