Detergent Types Research Articles

Experimental investigation of surfactant adsorption during EOR and soil pollution control processes: Influence of surfactant concentration, water salinity, and clay type

Surfactant injection is a well-established method of chemical EOR processes. Surfactant adsorption into clay layers can prevent their proper performance and thus reduce the oil recovery factor. On the other hand, this adsorption property of clay materials can be used to prevent surface and underground water pollution and reduce soil pollution. In this experimental study, the effect of surfactant concentration, electrolyte type (NaCl and MgCl2), and the solution salinity on fluid adsorption into the interlayer space of different clay types (bentonite and kaolinite) was investigated. XRF analysis was conducted on two relevant clay samples, and immersion and Washburn tests were performed on the desired samples with the Sigma 700 setup. Then, according to the clay type, the most optimal conditions were introduced for the surfactant solution used in the two areas of EOR and environmental processes related to reducing soil pollution. In the EOR processes, the optimal condition for the lowest adsorption amount is C (with 1 CMC concentration and salinity of 100,000 ppm for NaCl salt). This fluid works better in kaolinite formations. In the environmental field related to the reduction of soil pollution, if the pollutants we are looking for are R and S (with alkyl benzene sulfonic acid as the dominant agent), bentonite has a better performance than kaolinite in terms of adsorption and subsequently pollution control. If the polluting fluid contains MgCl2 ions in the exact salinity values, the adsorption amount and soil pollution control will be higher for both adsorbent clays than if our fluid has NaCl salinity. The study's findings have a wide range of applications in surfactant flooding designs, surfactant adsorption optimization, and can be generalized to other detergent types.

Adaptive Neuro-Fuzzy Inference System to Predict the Release of Microplastic Fibers during Domestic Washing

Abstract Microplastic particles are a burgeoning population crisis in marine environments. This work is to predict the release of microplastic fibers from jeans made from polyester during domestic washing by using of adaptive neuro-fuzzy inference system (ANFIS) model. The advantage of the ANFIS model is to predict the variations between the randomly chosen parameters. This prediction model can be cost-effective and slowed down to study behavior more closely. The consequence of washing duration, temperature, spin speed, detergent types, and conditioner usage was investigated against the microplastic fiber release. The washing temperature, washing duration, spin speed, detergent types, and addition of conditioner are the main factors for this research work. The forecast presentations have been exposed by having a considerably lowered root mean square error value of 3.23 compared with the variant of the experiment as exposed by its standard deviation for the ANFIS version. This ANFIS model will be able to provide a theoretical understanding to enhance and inhibit microplastic fiber release from jeans.

Assessing Detergent Residuals for Reusable Device Cleaning Validations.

Cleaning chemistries are detergent-based formulations that are used during the processing of reusable medical devices. Manufacturers are responsible for demonstrating the safety of cleaning formulations when they are used during a device processing cycle, including the risk of device-associated cytotoxicity over the concentration ranges for recommended use and rinsing during cleaning. However, no regulation currently exists requiring manufacturers to demonstrate such safety. Although manufacturers' safety data sheets (SDSs) provide information on the safe use of chemicals for users, this information may not provide sufficient detail to determine the risks of residual chemicals on device surfaces. SDSs are not required to contain a comprehensive list of chemicals used, only those of risk to the user. They should be supplemented with information on the correct concentrations that should be used for cleaning, as well as instructions on the rinsing required to reduce the levels of chemicals to safe (nontoxic) levels prior to further processing. Supporting data, such as toxicity profiles or cytotoxicity data that support the instructions for use, would provide medical device manufacturers and healthcare personnel with the necessary information to make informed decisions about selection and correct use of detergents. In the current work, cytotoxicity profiles for eight commonly used cleaning formulations available internationally were studied. Although all of these products are indicated for use in the cleaning of reusable medical devices, results vary across the serial dilution curves and are not consistent among detergent types. The information presented here can be leveraged by both medical device manufacturers and processing department personnel to properly assess residual detergent risks during processing. This work also serves as a call to cleaning formulation manufacturers to provide this information for all chemistries.

Assessing Detergent Residuals for Reusable Device Cleaning Validations

Cleaning chemistries are detergent-based formulations that are used during the processing of reusable medical devices. Manufacturers are responsible for demonstrating the safety of cleaning formulations when they are used during a device processing cycle, including the risk of device-associated cytotoxicity over the concentration ranges for recommended use and rinsing during cleaning. However, no regulation currently exists requiring manufacturers to demonstrate such safety. Although manufacturers' safety data sheets (SDSs) provide information on the safe use of chemicals for users, this information may not provide sufficient detail to determine the risks of residual chemicals on device surfaces. SDSs are not required to contain a comprehensive list of chemicals used, only those of risk to the user. They should be supplemented with information on the correct concentrations that should be used for cleaning, as well as instructions on the rinsing required to reduce the levels of chemicals to safe (nontoxic) levels prior to further processing. Supporting data, such as toxicity profiles or cytotoxicity data that support the instructions for use, would provide medical device manufacturers and healthcare personnel with the necessary information to make informed decisions about selection and correct use of detergents. In the current work, cytotoxicity profiles for eight commonly used cleaning formulations available internationally were studied. Although all of these products are indicated for use in the cleaning of reusable medical devices, results vary across the serial dilution curves and are not consistent among detergent types. The information presented here can be leveraged by both medical device manufacturers and processing department personnel to properly assess residual detergent risks during processing. This work also serves as a call to cleaning formulation manufacturers to provide this information for all chemistries.

Evaluation of microfiber release from jeans: the impact of different washing conditions

Microplastic particles are a burgeoning population crisis in the marine environment. This research examines the emission of microfibers from three different jeans (garments) during domestic washing. The jeans types, washing temperature, washing duration, spin speed, detergent types, and addition of conditioner are the main factors for this research work. The average length and diameter of the microfibers for the 100% PET jeans (jeans-P) has 7800 ± 4000 μm and 11.9±3.2 μm and for polyester/cotton jeans (jeans-PB) has 4900 ± 2200 μm 17.4±4.8 μm, respectively. The maximum microfiber released was observed in the rigorous washing treatment (90 min, 60°C, 1400 rpm, powder detergent with the presence of conditioner). The surmised number of microfibers discharged from the 1 kg wash load of jeans-P was calculated within the extent of 2300000–4900000 microfibers, and it is varied by the washing treatments.

Effect of detergent type on the performance of liver decellularized extracellular matrix-based bio-inks

Decellularized extracellular matrix-based bio-inks (dECM bio-inks) for bioprinting technology have recently gained attention owing to their excellent ability to confer tissue-specific functions and 3D-printing capability. Although decellularization has led to a major advancement in bio-ink development, the effects of detergent type, the most important factor in decellularization, are still unclear. In this study, the effects of various detergent types on bio-ink performance were investigated. Porcine liver-derived dECM bio-inks prepared using widely used detergents, including sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC), Triton X-100 (TX), and TX with ammonium hydroxide (TXA), were characterized in detail. SDS and SDC severely damaged glycosaminoglycan and elastin proteins, TX showed the lowest rate of decellularization, and TXA-based dECM bio-ink possessed the highest ECM content among all bio-inks. Differences in biochemical composition directly affected bio-ink performance, with TXA-dECM bio-ink showing the best performance with respect to gelation kinetics, intermolecular bonding, mechanical properties, and 2D/3D printability. More importantly, cytocompatibility tests using primary mouse hepatocytes also showed that the TXA-dECM bio-ink improved albumin secretion and cytochrome P450 activity by approximately 2.12- and 1.67-fold, respectively, compared with the observed values for other bio-inks. Our results indicate that the detergent type has a great influence on dECM damage and that the higher the dECM content, the better the performance of the bio-ink for 3D bioprinting.

Development and effects of naphthol dyes in the washing processes

This study aimed to: describe the value of color fastness to washing by using five types of detergent namely cream, powder, bar, liquid, and Sapindusrarak on Tan written batik cloth with naphthol dyes; determine the effect of the detergent types on the color fastness; and find out the best type of detergent according to its composition for washing batik with naphthol dyes. This was true experimental research with the AXB factorial design in which A is the Tan batik cloth, and B is the factor of washing treatment with five detergent types. To collect the data, the researchers employed the probability sampling technique, which is asimple random sampling technique. Also, they tested the color fastness in the washing processes carried out by the examining team at the Batik and Craft Center with procedures and calibrated tools.The data were analyzed using a non-parametric analysis, namely, a Kruskal-Wallis test. The color fastness test of Tan batik cloth washed using five types of detergent produces a good average value of 4- 4.5. The result of the difference test on thecolor fastness to washing and staining is p 0.406> 0.05. It means there is no significant difference becausethe detergents have the same composition, i.e., an anionic surfactant.The detergent with the best composition is Sapindusrarak, because it contains saponin as a natural surfactant. People can use detergents to wash batik by selectingthe composition of lauryl alkyl benzene sulfonate as a soft substance.

Influence of detergents types and clean method on PV cells performance: An experimental study

Accumulation the dust and dirt soiling on photovoltaic (PV) panels’ surfaces represents a major problem which inevitably undermines their operational performance. Thereby, removing the soiling and cleaning the panel surfaces is necessary to upgrade the PV cells performance. In this study the influence of detergent types as a cleaning material on PV panels’ outcomes has been investigated experimentally. Three detergents types that widely materials using in the home cleaning include: Tap water, mixture of dishwashing liquid with water and glass cleaner were tested. Furthermore, manual cleaning by using a piece of cloth or wiper were utilized as a cleaning tools were also evaluated. The experimental results appeared that enhancement the PV panel performance after using home detergents materials, where the performance increased by 25.35% and 19% when used a glasses cleaner and mixture of water and dishwashing liquid respectively, compared with 7.34% when used tap water only. In addition, using the manual wiper as a cleaning technique was achieved a better PV panel performance than piece of cloth for all detergents materials.

Activity-dependent changes in synaptic protein complex composition are consistent in different detergents despite differential solubility

At the post-synaptic density (PSD), large protein complexes dynamically form and dissociate in response to synaptic activity, comprising the biophysical basis for learning and memory. The use of detergents to isolate the PSD and release its membrane-associated proteins complicates studies of these activity-dependent protein interaction networks, because detergents can simultaneously disrupt the very interactions under study. Despite widespread recognition that different detergents yield different experimental results, the effect of detergent on activity-dependent synaptic protein complexes has not been rigorously examined. Here, we characterize the effect of three detergents commonly used to study synaptic proteins on activity-dependent protein interactions. We first demonstrate that SynGAP-containing interactions are more abundant in 1% Deoxycholate (DOC), while Shank-, Homer- and mGluR5-containing interactions are more abundant in 1% NP-40 or Triton. All interactions were detected preferentially in high molecular weight complexes generated by size exclusion chromatography, although the detergent-specific abundance of proteins in high molecular weight fractions did not correlate with the abundance of detected interactions. Activity-dependent changes in protein complexes were consistent across detergent types, suggesting that detergents do not isolate distinct protein pools with unique behaviors. However, detection of activity-dependent changes is more or less feasible in different detergents due to baseline solubility. Collectively, our results demonstrate that detergents affect the solubility of individual proteins, but activity-dependent changes in protein interactions, when detectable, are consistent across detergent types.

Cell-free expression, purification and characterization of Drosophila melanogaster odorant receptor OR42a and its co-receptor

Olfactory receptors (OR), a group of classic membrane proteins, plays a vital role in insect reproduction and acclimatization. Deciphering the molecular mechanism of insect olfaction could enhance pest control and environmental protection. Studies on ORs have faced a major bottleneck due to the notoriously strong hydrophobicity of ORs, which results in difficult expression in heterologous cell systems. Here, we demonstrated that insect ORs could be functionally produced using the E. coli cell-free protein synthesis system (CFPS), in which the highest yield of total ORs is 350 μg per 1 ml reaction. We tested the effects of detergent types and concentrations on soluble expression of ORs. The ORs showed a classic α-helical infrared spectrum. Quartz crystal microbalance (QCM) was used to demonstrate that ORs fold correctly and respond to their ligands. This is the first report that insect OR42a could be functionally produced in vitro. This approach may facilitate the development of biomimetic olfactory biosensors and may also be utilized for drug positioning and development, environmental protection and agriculture.

Large-scale in vitro production, refolding and dimerization of PsbS in different microenvironments

Plants adapt to fluctuating light conditions by a process called non-photochemical quenching (NPQ), where membrane protein PsbS plays a crucial role and transforms a change in the pH-gradient across the thylakoid membrane under excess light conditions into a photoprotective state, leading to de-excitation of antenna chlorophylls. The PsbS activation mechanism is elusive and has been proposed to involve a monomerization step and protonation of specific residues. To elucidate its function, it is essential to produce PsbS in large quantities, stabilize PsbS in a membrane-mimicking environment and analyze its pH-dependent conformational structure. We present an approach for large-scale in-vitro production and spectroscopic characterization of PsbS under controlled, non-crystalline conditions. We produced PsbS of the moss Physcomitrella patens in milligram quantities in E. coli, refolded PsbS in several detergent types and analyzed its conformation at neutral and low pH by Dynamic Light Scattering and NMR spectroscopy. Our results reveal that at both pH conditions, PsbS exist as dimers or in apparent monomer-dimer equilibria. Lowering of the pH induces conformational changes, destabilizes the dimer state and shifts the equilibria towards the monomeric form. In vivo, a similar response upon thylakoid lumen acidification may tune PsbS activity in a gradual manner.

Dodecyl Maltoside Protects Membrane Proteins In Vacuo

Molecular dynamics simulations have been used to characterize the effects of transfer from aqueous solution to a vacuum to inform our understanding of mass spectrometry of membrane-protein-detergent complexes. We compared two membrane protein architectures (an α-helical bundle versus a β-barrel) and two different detergent types (phosphocholines versus an alkyl sugar) with respect to protein stability and detergent packing. The β-barrel membrane protein remained stable as a protein-detergent complex in vacuum. Zwitterionic detergents formed conformationally destabilizing interactions with an α-helical membrane protein after detergent micelle inversion driven by dehydration in vacuum. In contrast, a nonionic alkyl sugar detergent resisted micelle inversion, maintaining the solution-phase conformation of the protein. This helps to explain the relative stability of membrane proteins in the presence of alkyl sugar detergents such as dodecyl maltoside.

RO membrane treatment of domestic grey-water containing different detergent types

Water reclamation from grey-water containing detergent and salinity (∼2,000 ppm) was carried out using indigenously developed brackish water TFC membrane module (4040 size) at 250 psi operating pressure. Reusable water with <300 ppm inorganic solutes and trace amounts of organic content of detergent origin was produced upon reverse osmosis (RO) treatment of grey-water. The RO module performance in terms of product flux was influenced by the detergent type present in the feed water. While the product flux was rather unchanged for the feed containing a commercial linear alkyl benzene-alfa olefin sulfonate-soda ash based detergent, a significant flux decline (∼25%) was observed for the feed containing a commercial C8–C24 primary/secondary ethoxylates based detergent. However, the membrane selectivity in terms of salt rejection was slightly higher for the feed with detergent than that of the detergent-free feed. This can be due to the surface-active agent of the detergent which alters the surface potential of the membrane. This is in agreement with the changes observed by atomic force microscopy (AFM) and the Zeta-potential measurements.

Detergent selection for enhanced extraction of membrane proteins

Generating stable conditions for membrane proteins after extraction from their lipid bilayer environment is essential for subsequent characterization. Detergents are the most widely used means to obtain this stable environment; however, different types of membrane proteins have been found to require detergents with varying properties for optimal extraction efficiency and stability after extraction. The extraction profiles of several detergent types have been examined for membranes isolated from bacteria and yeast, and for a set of recombinant target proteins. The extraction efficiencies of these detergents increase at higher concentrations, and were shown to correlate with their respective CMC values. Two alkyl sugar detergents, octyl-β-d-glucoside (OG) and 5-cyclohexyl-1-pentyl-β-d-maltoside (Cymal-5), and a zwitterionic surfactant, N-decylphosphocholine (Fos-choline-10), were generally effective in the extraction of a broad range of membrane proteins. However, certain detergents were more effective than others in the extraction of specific classes of integral membrane proteins, offering guidelines for initial detergent selection. The differences in extraction efficiencies among this small set of detergents supports the value of detergent screening and optimization to increase the yields of targeted membrane proteins.

Structure formation in binary mixtures of surfactants: vesicle opening-up to bicelles and octopus-like micelles

Micelle formation in binary mixtures of surfactants is studied using a coarse-grained molecular simulation. When a vesicle composed of lipid and detergent types of molecules is ruptured, a disk-shaped micelle, the bicelle, is typically formed. It is found that cup-shaped vesicles and bicelles connected with worm-like micelles are also formed depending on the surfactant ratio and critical micelle concentration. The obtained octopus shape of micelles agree with those observed in the cryo-TEM images reported in [S. Jain and F. S. Bates, Macromol. 37, 1511 (2004).]. Two types of connection structures between the worm-like micelles and the bicelles are revealed.

Expression, purification and in vitro functional reconstitution of the chemokine receptor CCR1

Chemokine receptors are a specific class of G-protein-coupled receptors (GPCRs) that control cell migration associated with routine immune surveillance, inflammation and development. In addition to their roles in normal physiology, these receptors and their ligands are involved in a large number of inflammatory diseases, cancer and AIDS, making them prime therapeutic targets in the pharmaceutical industry. Like other GPCRs, a significant obstacle in determining structures and characterizing mechanisms of activation has been the difficulty in obtaining high levels of pure, functional receptor. Here we describe a systematic effort to express the chemokine receptor CCR1 in mammalian cells, and to purify and reconstitute it in functional form. The highest expression levels were obtained using an inducible HEK293 system. The receptor was purified using a combination of N- (StrepII or Hemagglutinin) and C-terminal (His8) affinity tags. Function was assessed by ligand binding using a novel fluorescence polarization assay with fluorescein-labeled chemokine. A strict dependence of function on the detergent composition was observed, as solubilization of CCR1 in n-dodecyl-β- d-maltopyranoside/cholesteryl hemisuccinate yielded functional receptor with a K d of 21 nM for the chemokine CCL14, whereas it was non-functional in phosphocholine detergents. Differences in function were observed despite the fact that both these detergent types maintained the receptor in a state characterized by monomers and small oligomers, but not large aggregates. While optimization is still warranted, yields of ∼0.1–0.2 mg of pure functional receptor per 10 9 cells will permit biophysical studies of this medically important receptor.

Effects of Laundering on Conductivity of Polypyrrole-Coated Textiles

This study examines the resistance change in conductive polypyrrole-coated PET fabrics under the AS 2001.4.15 – 1994 laundering test conditions. The effects of individual detergent components of a standard detergent, including auxiliary chemicals, at four different temperatures were studied. When the coated fabric was washed under the AS 2001.4.15-1994 conditions, the ECE reference detergent and pure soap flakes (sodium sterate) both decreased the conductivity of the coating at a rate exponentially proportional to the laundering temperature. Detergent types had an influence over the rate of degradation; pH conditions had a large influence on the rate of polymer deterioration with the acidic nonionic detergent giving rise to significantly improved laundering conditions. The auxiliary chemicals, sodium carbonate and sodium perborate were seen to cause large degradation of polymers during laundering. Ethylene diamine tetra acetic acid was seen to have only a slight influence on the reduction of conductivity of polymers.

The impact of detergent chemistry on TBN retention

AbstractHistorically, the characterisation of fresh and used diesel engine lubricants has been based on a limited number of analytical techniques. One of the most important methods of analysis has been total base number (TBN) measurement. Although TBN measurements are informative, easy, and quick, it can be misleading to base the judgement of an oil's performance solely on one criterion.This paper offers some observations from a field test, showing that some detergent types gave unacceptable performance even though the TBNs were at an acceptable level. It is hypothesised that some detergents do not effectively neutralise all acidic species present in the lubricant, thereby reserving their own base, while in fact the oil may no longer provide sufficient protection against bearing corrosion. This hypothesis is supported by bench and engine test data. It is recommended that, at a minimum, total acid number (TAN) measurements be included in any analysis, and where time and cost permit, wear metals content, oxidation, soot content, and viscosity should also be evaluated.

Overbased lubricant detergents – a comparative study

AbstractOverbased detergents are commonly used in automotive and marine lubricants. Their main functions are to neutralise potentially corrosive acids and to contribute to engine cleanliness. The major types of overbased detergents that are produced commercially are phenates, sulphonates and salicylates. This paper describes the chemistry of these additive classes and reviews their properties and performance in bench and engine tests. The paper also describes a new class of overbased detergents, where novel chemistry has been used to produce new types of highly overbased phenate, sulphonate, and salicylate. These new products exhibit significant improvements in properties and performance over conventional detergents. Evidence from bench and engine tests is presented to demonstrate the benefits of the new detergent types. An appendix gives more detail on the results of tribological tests on these new detergents.

Comparison of After-Laundering Residues of Cypermethrin and Cyfluthrin in Fabrics: A Meta-Analytical Approach

This study is a meta-analysis of four experiments with two pyrethroids, cypermethrin and cyfluthrin, in two formulations, emulsifiable concentrates and wettable powders. Contributions of fabric weight, detergent type, and detergent concentration are evaluated for residue remaining in fabrics after laundering. Each of the four experiments is a factorial experiment, with two fabric weights (10 ozlyd2 and 14.5 ozlyd2), two detergent types (heavy-duty liquid and phosphate) and four detergent concentrations ( 0.0x, 0.5x, 1.0x, and 1.5x, where x = package label recommendations). Fabric specimens were contaminated, laundered, and residues remaining after laundering determined gas chromatographically. The heavier the fabric, the more residues were retained. Cypermethrin was more completely removed by laundering than was cyfluthrin. The emulsifiable concentrate formulation was more difficult to remove than the wettable-powder formulation. The cyfluthrin emulsifiable concentrate residue was greater following laundering than other pesticides. Increasing detergent concentration was effective in reducing cyfluthrin residue. Detergent type should be selected according to pesticide formulation: a heavy-duty liquid detergent for the emulsifiable-concentrate and a phosphate, powdered detergent for the wettable-powder.